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Protein Page:
IFI16 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
IFI16 a nuclear protein that may function as a transcriptional repressor. Strongly induced by gamma interferon and, to a lesser extent, by alpha interferon. Binds double-stranded DNA and cell cycle regulatory factors including p53 and Rb. Loss of IFI16 activates p53 checkpoint through NBS1-DNAPK pathway. Inhibits cell growth in the Ras/Raf signaling pathway. May be involved in the senescence of prostate epithelial cells. Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prostate, ovary and breast. Four alternatively spliced isoforms have been described. Isoforms-1, -2 and -3 can homo- and hetero-dimerize. Note: This description may include information from UniProtKB.
Protein type: Transcription factor
Chromosomal Location of Human Ortholog: 1q22
Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nuclear speck; nucleus; cytosol
Molecular Function: identical protein binding; protein binding; double-stranded DNA binding; transcription factor binding
Biological Process: positive regulation of cytokine production; transcription, DNA-dependent; monocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of DNA binding; cellular response to glucose starvation; positive regulation of interleukin-1 beta production; cell proliferation; negative regulation of viral genome replication; negative regulation of innate immune response; positive regulation of interferon type I production; autophagy; innate immune response; myeloid cell differentiation; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; regulation of autophagy; negative regulation of transcription, DNA-dependent; inflammatory response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; defense response to virus; activation of innate immune response
Reference #:  Q16666 (UniProtKB)
Alt. Names/Synonyms: Gamma-interferon-inducible protein 16; IF16; Ifi-16; IFI16; IFNGIP1; interferon, gamma-inducible protein 16; interferon-gamma induced protein IFI 16; Interferon-inducible myeloid differentiation transcriptional activator; MGC9466; PYHIN2
Gene Symbols: IFI16
Molecular weight: 88,256 Da
Basal Isoelectric point: 9.31  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

IFI16

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K34-ub SLLSNDLkLNLKMRE
1 0 K45-ac KMREEYDkIQIADLM
0 4 K64-ub RGDAGLGkLIkIFED
0 1 K67-ub AGLGkLIkIFEDIPT
0 3 K83-ub EDLAETLkKEkLKVk
0 2 K86-ac AETLkKEkLKVkGPA
0 1 K90-ub kKEkLKVkGPALsRK
1 7 S95-p KVkGPALsRKRkkEV
1 0 K99-ac PALsRKRkkEVDATs
0 3 K99-ub PALsRKRkkEVDATs
0 6 K100-ub ALsRKRkkEVDATsP
1 24 S106-p kkEVDATsPAPsTsS
0 2 S110-p DATsPAPsTsSTVKt
0 2 S112-p TsPAPsTsSTVKtEG
0 1 T117-p sTsSTVKtEGAEAtP
0 5 T123-p KtEGAEAtPGAQkRK
1 11 K128-ac EAtPGAQkRKKsTKE
0 2 K128-ub EAtPGAQkRKKsTKE
1 1 K131 PGAQkRKKsTKEKAG
1 4 S132-p GAQkRKKsTKEKAGP
0 2 S142-p EKAGPKGsKVSEEQt
0 3 T149-p sKVSEEQtQPPsPAG
1 37 S153-p EEQtQPPsPAGAGMs
0 1 S160-p sPAGAGMstAMGRsP
0 4 T161-p PAGAGMstAMGRsPs
0 16 S166-p MstAMGRsPsPKTSL
1 8 S168-p tAMGRsPsPKTSLsA
0 1 T171 GRsPsPKTSLsAPPN
0 1 L173 sPsPKTSLsAPPNSS
1 0 S174-p PsPKTSLsAPPNSSS
0 1 K190-ub ENPKTVAkCQVtPRR
0 3 T194-p TVAkCQVtPRRNVLQ
1 1 K214-ac VKVLSTTkPFEYETP
0 1 T232-p KKIMFHAtVATQtQF
0 1 T237-p HAtVATQtQFFHVKV
0 3 K250-ub KVLNTSLkEKFNGKK
0 1 Y317-p QASGNIVyGVFMLHK
0 2 T428-p FPESHLRtPQMPPTt
0 12 T435-p tPQMPPTtPssSFFT
0 1 S437-p QMPPTtPssSFFTKk
0 1 S438-p MPPTtPssSFFTKkS
1 0 K444-ac ssSFFTKkSEDTISk
1 0 K451-ac kSEDTISkMNDFMRM
0 3 S480-p TSIGPAEsHPHtPQM
0 4 T484-p PAEsHPHtPQMPPst
0 1 S490-p HtPQMPPstPSSSFL
0 1 T491-p tPQMPPstPSSSFLT
0 3 S536-p TSIGPAEsHPHtPQM
0 9 T540-p PAEsHPHtPQMPPst
0 6 S546-p HtPQMPPstPsSSFL
0 1 T547-p tPQMPPstPsSSFLT
0 1 S549-p QMPPstPsSSFLTTL
1 1 K561-ac TTLKPRLktEPEEVs
0 5 K561-ub TTLKPRLktEPEEVs
1 2 K561-sm TTLKPRLktEPEEVs
0 7 T562-p TLKPRLktEPEEVsI
0 1 S568-p ktEPEEVsIEDSAQs
0 2 S575-p sIEDSAQsDLKEVMV
1 0 K598-ac YEPKEQKkMFHATVA
1 0 K614-ac ENEVFRVkVFNIDLk
0 3 K614-ub ENEVFRVkVFNIDLk
0 3 K621-ub kVFNIDLkEKFTPKK
0 4 S670-p KGLIRSAsVTPkINQ
0 4 K674-ub RSAsVTPkINQLCSQ
0 2 K695-ub NGVFEVHkKNVRGEF
0 2 Y704-p NVRGEFTyYEIQDNT
0 1 K732-ub INCEEGDkLKLTCFE
0 17 K743-ub TCFELAPkSGNTGEL
0 3 S775-p KDILNPDssMEtsPD
0 3 S776-p DILNPDssMEtsPDF
0 1 T779-p NPDssMEtsPDFFF_
1 16 S780-p PDssMEtsPDFFF__
  IFI16 iso2  
K34 SLLSNDLKLNLKMRE
K45 KMREEYDKIQIADLM
K64 RGDAGLGKLIKIFED
K67 AGLGKLIKIFEDIPT
K83 EDLAETLKKEKLKVK
K86 AETLKKEKLKVKGPA
K90 KKEKLKVKGPALSRK
S95 KVKGPALSRKRKKEV
K99 PALSRKRKKEVDATS
K99 PALSRKRKKEVDATS
K100 ALSRKRKKEVDATSP
S106 KKEVDATSPAPSTSS
S110 DATSPAPSTSSTVKT
S112 TSPAPSTSSTVKTEG
T117 STSSTVKTEGAEATP
T123 KTEGAEATPGAQKRK
K128 EATPGAQKRKKSTKE
K128 EATPGAQKRKKSTKE
K131 PGAQKRKKSTKEKAG
S132 GAQKRKKSTKEKAGP
S142 EKAGPKGSKVSEEQT
T149 SKVSEEQTQPPSPAG
S153 EEQTQPPSPAGAGMS
S160 SPAGAGMSTAMGRSP
T161 PAGAGMSTAMGRSPS
S166 MSTAMGRSPSPKTSL
S168 TAMGRSPSPKTSLSA
T171 GRSPSPKTSLSAPPN
L173 SPSPKTSLSAPPNSS
S174 PSPKTSLSAPPNSSS
K190 ENPKTVAKCQVTPRR
T194 TVAKCQVTPRRNVLQ
K214 VKVLSTTKPFEYETP
T232 KKIMFHATVATQTQF
T237 HATVATQTQFFHVKV
K250 KVLNTSLKEKFNGKK
Y317 QASGNIVYGVFMLHK
T428 FPESHLRTPQMPPTT
T435 TPQMPPTTPSSSFFT
S437 QMPPTTPSSSFFTKK
S438 MPPTTPSSSFFTKKS
K444 SSSFFTKKSEDTISK
K451 KSEDTISKMNDFMRM
- gap
- gap
- gap
- gap
S480 TSIGPAESHPHTPQM
T484 PAESHPHTPQMPPST
S490 HTPQMPPSTPSSSFL
T491 TPQMPPSTPSSSFLT
S493 QMPPSTPSSSFLTTL
K505 TTLKPRLKTEPEEVS
K505 TTLKPRLKTEPEEVS
K505 TTLKPRLKTEPEEVS
T506 TLKPRLKTEPEEVSI
S512 KTEPEEVSIEDSAQS
S519 SIEDSAQSDLKEVMV
K542 YEPKEQKKMFHATVA
K558 ENEVFRVKVFNIDLK
K558 ENEVFRVKVFNIDLK
K565 KVFNIDLKEKFTPKK
S614 KGLIRSASVTPKINQ
K618 RSASVTPKINQLCSQ
K639 NGVFEVHKKNVRGEF
Y648 NVRGEFTYYEIQDNT
K676 INCEEGDKLKLTCFE
K687 TCFELAPKSGNTGEL
S719 KDILNPDSSMETSPD
S720 DILNPDSSMETSPDF
T723 NPDSSMETSPDFFF_
S724 PDSSMETSPDFFF__
  mouse

 
N34 SLLARDLNLERDNQE
T45 DNQEQYTTIQIANMM
K64 PADSGLGKLIEFCEE
E67 SGLGKLIEFCEEVPA
K83 RKRAEILKKERSEVT
R86 AEILKKERSEVTGET
- gap
S94-p SEVTGETsLEKNGQE
G99 ETsLEKNGQEAGPAt
G99 ETsLEKNGQEAGPAt
Q100 TsLEKNGQEAGPAtP
T106-p GQEAGPAtPTStTsH
T110-p GPAtPTStTsHMLAS
S112-p AtPTStTsHMLASER
T164 TSTAQARTSTAQEGT
T171 TSTAQEGTSTAQkRK
K176-ac EGTSTAQkRKsMREE
K176 EGTSTAQKRKsMREE
S179-p STAQkRKsMREEETG
M180 TAQkRKsMREEETGV
S190 EETGVKKSKAAKEPD
D197 SKAAKEPDQPPCCEE
C201 KEPDQPPCCEEPTAM
- under review  
T206 PPCCEEPTAMCQsPI
S211-p EPTAMCQsPILHsSs
I213 TAMCQsPILHsSsSA
S216-p CQsPILHsSsSASSN
S218-p sPILHsSsSASSNIP
S219 PILHsSsSASSNIPS
Q235 KNQKSQPQNQNIPRG
I239 SQPQNQNIPRGAVLH
D259 VMVLTATDPFEYESP
T277 VKNMLHATVATVSQY
S282 HATVATVSQYFHVKV
K295 KVFNINLKEKFTKKN
Y362 GTSGAVFYGVFTLHK
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
A444 GNVPKEPAKEEDHHH
- gap
R474 YDLKEDKRMFHATVA
K490 ETEFFRVKVFDTALK
K490 ETEFFRVKVFDTALK
K497 KVFDTALKSKFIPRN
N546 NTLRQRANATPKISY
K550 QRANATPKISYLFSQ
K571 SGEYLVNKKTERNKF
Y580 TERNKFIYYGIGDDT
K608 VRCEPGSKLRLVCFE
- gap
- gap
- gap
- gap
- gap
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