Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
MCM3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MCM3 a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for DNA replication and cell proliferation. Note: This description may include information from UniProtKB.
Protein type: DNA replication; EC 3.6.4.12
Cellular Component: nucleoplasm; centrosome; alpha DNA polymerase:primase complex; MCM complex; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm; nucleolus; nucleus
Molecular Function: protein binding; DNA helicase activity; DNA binding; ATP binding
Biological Process: DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; DNA duplex unwinding; G1/S transition of mitotic cell cycle
Reference #:  P25205 (UniProtKB)
Alt. Names/Synonyms: cervical cancer proto-oncogene 5; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor MCM3; HCC5; hRlf beta subunit; MCM3; MCM3 minichromosome maintenance deficient 3; MGC1157; minichromosome maintenance complex component 3; minichromosome maintenance deficient 3; P1-MCM3; P1.h; p102; replication licensing factor, beta subunit; RLF subunit beta; RLFB
Gene Symbols: MCM3
Molecular weight: 90,981 Da
Basal Isoelectric point: 5.53  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MCM3

Protein Structure Not Found.


STRING  |  Wikipedia  |  Reactome  |  neXtProt  |  Protein Atlas  |  BioGPS  |  Scansite  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
cell cycle regulation: T722‑p
molecular association, regulation: T722‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 T4-p ____MAGtVVLDDVE
0 2 Y19-p LREAQRDyLDFLDDE
0 2 Y32-p DEEDQGIyQskVREL
0 1 S34-p EDQGIyQskVRELIS
0 3 K35-ub DQGIyQskVRELISD
2 0 S112-p SFGSKHVsPRTLTSC
0 1 K152-ac VHYCPATkKTIERRy
0 1 K152-ub VHYCPATkKTIERRy
0 9 Y159-p kKTIERRysDLttLV
1 30 S160-p KTIERRysDLttLVA
0 3 T163-p ERRysDLttLVAFPs
0 2 T164-p RRysDLttLVAFPss
0 1 S170-p ttLVAFPssSVYPtK
0 1 S171-p tLVAFPssSVYPtKD
0 1 T176-p PssSVYPtKDEENNP
0 1 K207-ub TIQEMPEkAPAGQLP
0 1 R234 DKAKPGDRVQVVGtY
0 1 T240-p DRVQVVGtYRCLPGK
0 1 R242 VQVVGtYRCLPGKKG
0 1 T252-p PGKKGGYtSGtFRTV
0 3 T255-p KGGYtSGtFRTVLIA
0 1 T258 YtSGtFRTVLIACNV
0 1 S269 ACNVKQMSKDAQPsF
0 2 S275-p MSKDAQPsFSAEDIA
0 1 K293-ub KFSKTRSkDIFDQLA
0 1 K301-ub DIFDQLAkSLAPSIH
0 2 K351-ub IGDPSVAkSQLLRYV
0 16 K435-ub QGRVTIAkAGIHARL
0 2 K463-ub YGRYDQYkTPMENIG
0 1 S474-p ENIGLQDsLLSRFDL
1 2 S535-p ATDDPNFsQEDQQDT
0 3 Y545-p DQQDTQIyEKHDNLL
0 1 N550 QIyEKHDNLLHGtkK
0 1 T555-p HDNLLHGtkKKkEKM
0 2 K556-ac DNLLHGtkKKkEKMV
0 1 K556-sc DNLLHGtkKKkEKMV
0 1 K559-ac LHGtkKKkEKMVSAA
0 1 K579-ub IHVAKIIkPVLTQES
0 1 S599-p EEYSRLRsQDSMSSD
1 1 S611-p SSDTARTsPVTARtL
0 1 T617-p TsPVTARtLETLIRL
0 1 Y651-p EAVELVQyAYFKKVL
0 30 S668-p EKKRKKRsEDEsEtE
0 139 S672-p KKRsEDEsEtEDEEE
0 115 T674-p RsEDEsEtEDEEEKs
0 21 S681-p tEDEEEKsQEDQEQK
0 8 S704-p PDAKDGDsyDPyDFs
0 3 Y705-p DAKDGDsyDPyDFsD
0 28 Y708-p DGDsyDPyDFsDtEE
0 101 S711-p syDPyDFsDtEEEMP
0 61 T713-p DPyDFsDtEEEMPQV
0 3 E715 yDFsDtEEEMPQVHt
2 92 T722-p EEMPQVHtPKtADsQ
0 5 T725-p PQVHtPKtADsQETk
1 4 S728-p HtPKtADsQETkEsQ
0 3 - gap
1 6 - gap
0 2 T731 KtADsQETkEsQKVE
0 1 K732-ub tADsQETkEsQKVEL
0 4 S734-p DsQETkEsQKVELsE
0 1 K736 QETkEsQKVELsEsR
0 1 S740-p EsQKVELsEsRLKAF
0 1 S742-p QKVELsEsRLKAFkV
0 1 K748-ub EsRLKAFkVALLDVF
12686 : Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb
  mouse

 
T4 ____MAGTVVLDDVE
Y19 LREAQRDYLDFLDDE
Y32 DEEDQGIYQNKVREL
N34 EDQGIYQNKVRELIS
K35 DQGIYQNKVRELISD
S112-p SFGSKHVsPRTLTSC
K152 VHYCPATKKTIERRy
K152 VHYCPATKKTIERRy
Y159-p KKTIERRysDLtTLV
S160-p KTIERRysDLtTLVA
T163-p ERRysDLtTLVAFPS
T164 RRysDLtTLVAFPSS
S170 tTLVAFPSSSVYPTK
S171 TLVAFPSSSVYPTKD
T176 PSSSVYPTKDEENNP
K207 TIQEMPEKAPAGQLP
R234-m1 DKVKPGDrIQVVGTY
T240 DrIQVVGTYrCLPGK
R242-m1 IQVVGTYrCLPGKKG
T252 PGKKGCYTSGTFRtV
T255 KGCYTSGTFRtVLIA
T258-p YTSGTFRtVLIACNV
S269-p ACNVKQMsKDIQPAF
A275 MsKDIQPAFSADDIA
K293 KFSKTRSKDVFEQLA
R301 DVFEQLARSLAPSIH
K351 IGDPSVAKSQLLRYV
K435-ub QGRVTIAkAGIHARL
K463 YGRYDQYKTPMENIG
S474 ENIGLQDSLLSRFDL
T535 ATDDPDFTQDDQQDT
Y545 DQQDTRIYEKHDsLL
S550-p RIYEKHDsLLHGTKK
T555 HDsLLHGTKKKKEKM
K556 DsLLHGTKKKKEKMV
K556 DsLLHGTKKKKEKMV
K559 LHGTKKKKEKMVSAA
K579 IHVAKIIKPTLTQES
S599 EEYSRLRSQDSMSSD
S611-p SSDTARTsPVTARTL
T617 TsPVTARTLETLIRL
Y651 EAVELVQYAYFKKVL
S668-p EKKRKKAsEDEsDLE
S672-p KKAsEDEsDLEDEEE
L674 AsEDEsDLEDEEEKs
S681-p LEDEEEKsQEDTEQK
S701-p THAKDGEsYDPyDFs
Y702 HAKDGEsYDPyDFsE
Y705-p DGEsYDPyDFsEAEt
S708-p sYDPyDFsEAEtQMP
A710 DPyDFsEAEtQMPQV
T712-p yDFsEAEtQMPQVHt
T719-p tQMPQVHtPKtDDsQ
T722-p PQVHtPKtDDsQEKt
S725-p HtPKtDDsQEKtDDs
T729-p tDDsQEKtDDsQEtQ
S732-p sQEKtDDsQEtQDsQ
T735-p KtDDsQEtQDsQkVE
Q736 tDDsQEtQDsQkVEL
S738-p DsQEtQDsQkVELSE
K740-ub QEtQDsQkVELSEPR
S744 DsQkVELSEPRLKAF
P746 QkVELSEPRLKAFKA
K752 EPRLKAFKAALLEVF
12686 : Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb
  rat

 
T4 ____MAGTVVLDDVE
Y19 LREAQRDYLDFLDDE
Y32 DEEDQGIYQNKVREL
N34 EDQGIYQNKVRELIS
K35 DQGIYQNKVRELISD
S112 SFGSKHVSPRTLTSC
K152 VHYCPATKKTIERRY
K152 VHYCPATKKTIERRY
Y159 KKTIERRYSDLTTLV
S160 KTIERRYSDLTTLVA
T163 ERRYSDLTTLVAFPS
T164 RRYSDLTTLVAFPSS
S170 TTLVAFPSSSVYPTK
S171 TLVAFPSSSVYPTKD
T176 PSSSVYPTKDEENNP
K207 TIQEMPEKAPAGQLP
R234 DKVKPGDRIQVVGTY
T240 DRIQVVGTYRCLPGK
R242 IQVVGTYRCLPGKKG
T252 PGKKGGYTSGTFRTV
T255 KGGYTSGTFRTVLIA
T258 YTSGTFRTVLIACNV
S269 ACNVKQMSKDIQPAF
A275 MSKDIQPAFSADDIA
K293 KFSKTRSKDVFEQLA
R301 DVFEQLARSLAPSIH
K351 IGDPSVAKSQLLRYV
K436 QGRVTIAKAGIHARL
K464 YGRYDQYKTPMENIG
S475 ENIGLQDSLLSRFDL
T536 ATDDPDFTQDDQQDT
Y546 DQQDTRIYEKHDNLL
N551 RIYEKHDNLLHGskK
S556-p HDNLLHGskKKREKM
K557-ac DNLLHGskKKREKMV
K557 DNLLHGsKKKREKMV
R560 LHGskKKREKMVSAA
K580 IHVAKIIKPTLTQES
S600 EEYSRLRSQDSMSSD
S612 SSDTARTSPVTARTL
T618 TSPVTARTLETLIRL
Y652 EAVELVQYAYFKKVL
S669-p EKKRKKPsEDEsDLE
S673-p KKPsEDEsDLEDEEE
L675 PsEDEsDLEDEEEKS
S682 LEDEEEKSQEEDTEQ
S703 TQAKDGESYDPYDFs
Y704 QAKDGESYDPYDFsE
Y707 DGESYDPYDFsEAER
S710-p SYDPYDFsEAEREMP
A712 DPYDFsEAEREMPQV
R714 YDFsEAEREMPQVHt
T721-p REMPQVHtPKtDDsQ
T724-p PQVHtPKtDDsQEKT
S727-p HtPKtDDsQEKTDES
T731 tDDsQEKTDESQETQ
S734 sQEKTDESQETQESQ
T737 KTDESQETQESQKVE
Q738 TDESQETQESQKVEL
S740 ESQETQESQKVELSE
K742 QETQESQKVELSESR
S746 ESQKVELSESRLKAF
S748 QKVELSESRLKAFKA
K754 ESRLKAFKAALLEVF
12686 : Phospho-MCM3 (Ser112) (D3S4M) Rabbit mAb
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.