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Protein Page:
ATP1A1 (rat)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ATP1A1 the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na and K ions across the plasma membrane. This action creates the electrochemical gradient of Na and K, providing the energy for active transport of various nutrients. Two splice-variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: EC 3.6.3.9; Membrane protein, multi-pass; Transporter, ion channel; Membrane protein, integral; Hydrolase; Transporter
Cellular Component: Golgi apparatus; protein complex; endoplasmic reticulum; basolateral plasma membrane; T-tubule; integral to membrane; caveola; lipid raft; membrane; apical plasma membrane; melanosome; plasma membrane; sodium:potassium-exchanging ATPase complex; sarcolemma; endosome
Molecular Function: potassium ion binding; protein domain specific binding; sodium ion binding; protein binding; chaperone binding; phosphoric monoester hydrolase activity; sodium:potassium-exchanging ATPase activity; phosphoinositide 3-kinase binding; ankyrin binding; ADP binding; protein kinase binding; ATP binding
Biological Process: ATP catabolic process; response to drug; negative regulation of heart contraction; sodium ion transport; membrane hyperpolarization; positive regulation of striated muscle contraction; regulation of sodium ion transport; negative regulation of glucocorticoid biosynthetic process; energy coupled proton transport, against electrochemical gradient; dephosphorylation; potassium ion import; ATP biosynthetic process; regulation of blood pressure; positive regulation of heart contraction; regulation of the force of heart contraction; potassium ion transport
Reference #:  P06685 (UniProtKB)
Alt. Names/Synonyms: AT1A1; Atp1a1; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; Na(+)/K(+) ATPase alpha-1 subunit; Nkaa1b; Sodium pump subunit alpha-1; Sodium/potassium-transporting ATPase subunit alpha-1
Gene Symbols: Atp1a1
Molecular weight: 113,054 Da
Basal Isoelectric point: 5.3  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ATP1A1

Protein Structure Not Found.


STRING  |  Reactome  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  UCSD-Nature  |  UniProtKB  |  Entrez-Gene


Sites Implicated In
exocytosis, altered: Y542‑p
activity, induced: Y10‑p
activity, inhibited: S943‑p
enzymatic activity, induced: S16‑p, S23‑p, Y542‑p
enzymatic activity, inhibited: S16‑p
intracellular localization: S16‑p, S23‑p
molecular association, regulation: S16‑p, S23‑p
protein conformation: S16‑p, S23‑p
protein degradation: S16‑p
receptor internalization, induced: S23‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       rat

 
0 14 K9 GKGVGRDKyEPAAVs
1 180 Y10-p KGVGRDKyEPAAVsE
12 9 S16-p KyEPAAVsEHGDkks
1 5 K21-ub AVsEHGDkkskkAKK
1 1 K22-ub VsEHGDkkskkAKKE
19 1 S23-p sEHGDkkskkAKKER
1 0 K24-ub EHGDkkskkAKKERD
1 0 K25-ub HGDkkskkAKKERDM
0 1 K36 ERDMDELKKEVSMDD
0 2 K37 RDMDELKKEVSMDDH
0 1 S40 DELKKEVSMDDHKLs
0 2 K45 EVSMDDHKLsLDELH
0 8 S47-p SMDDHKLsLDELHRK
0 2 K54 sLDELHRKyGTDLSR
1 66 Y55-p LDELHRKyGTDLSRG
0 1 T57 ELHRKyGTDLSRGLT
0 1 S60 RKyGTDLSRGLTPAR
0 2 K156 YQEAKSSKIMESFkN
0 22 K162-ub SKIMESFkNMVPQQA
0 14 K177-ub LVIRNGEkMSINAED
0 2 K194 VGDLVEVKGGDRIPA
0 1 S207 PADLRIISANGCkVD
0 11 K212-ub IISANGCkVDNSsLT
0 2 S216 NGCkVDNSsLTGEsE
0 22 S217-p GCkVDNSsLTGEsEP
0 1 T219 kVDNSsLTGEsEPQT
0 7 S222-p NSsLTGEsEPQTRSP
0 3 T226 TGEsEPQTRSPDFTN
0 9 S228 EsEPQTRSPDFTNEN
0 1 S246 TRNIAFFSTNCVEGT
1 1313 Y260-p TARGIVVyTGDRTVM
0 15 T261 ARGIVVyTGDRTVMG
0 2 T265 VVyTGDRTVMGRIAT
0 1 S275 GRIATLASGLEGGQT
0 2 K359 ARKNCLVKNLEAVET
0 1 K377 TSTICSDKTGTLTQN
0 6 K377 TSTICSDKTGTLTQN
0 1 S407-p ADTTENQsGVSFDKT
0 1 K413 QsGVSFDKTSATWFA
0 17 K444-ub QENLPILkRAVAGDA
0 3 S452 RAVAGDASESALLKC
0 1 S454 VAGDASESALLKCIE
0 11 M468 EVCCGSVMEMREKyT
1 0 Y474-p VMEMREKyTKIVEIP
0 2 K476 EMREKyTKIVEIPFN
0 2 S484 IVEIPFNSTNKYQLS
0 1 T485 VEIPFNSTNKYQLSI
0 1 K487 IPFNSTNKYQLSIHK
0 3 K487 IPFNSTNKYQLSIHK
0 2 K494 KYQLSIHKNPNASEP
0 2 K494 KYQLSIHKNPNASEP
0 1 A498 SIHKNPNASEPKHLL
0 2 S499 IHKNPNASEPKHLLV
0 1 K502 NPNASEPKHLLVMKG
0 1 K502 NPNASEPKHLLVMKG
0 3 K508 PKHLLVMKGAPERIL
0 3 K526-ub SSILLHGkEQPLDEE
0 5 K535 QPLDEELKDAFQNAy
0 1 K535 QPLDEELKDAFQNAy
1 120 Y542-p KDAFQNAyLELGGLG
0 64 K605-ub AVPDAVGkCRSAGIK
0 3 T617 GIKVIMVTGDHPITA
0 3 K625 GDHPITAKAIAKGVG
0 4 K629 ITAKAIAKGVGIISE
0 3 S635 AKGVGIISEGNETVE
0 1 - gap
0 1 - gap
0 4 T640 IISEGNETVEDIAAR
0 6 N653 ARLNIPVNQVNPRDA
0 10 K661-ub QVNPRDAkACVVHGS
0 2 S668 kACVVHGSDLkDMTS
0 6 K671-ub VVHGSDLkDMTSEEL
0 2 S675 SDLkDMTSEELDDIL
0 12 R683 EELDDILRyHTEIVF
1 27 Y684-p ELDDILRyHTEIVFA
0 3 T686 DDILRyHTEIVFART
0 2 K698 ARTSPQQKLIIVEGC
0 4 S722 TGDGVNDSPALkkAD
0 3 K726-ac VNDSPALkkADIGVA
0 5 K726-ub VNDSPALkkADIGVA
0 1 K727-ac NDSPALkkADIGVAM
0 7 K727-ub NDSPALkkADIGVAM
0 2 K773 RLIFDNLKKSIAYTL
0 28 K833 QAESDIMKRQPRNPK
0 24 K840 KRQPRNPKTDkLVNE
0 8 K843-ub PRNPKTDkLVNERLI
6 1 S943-p ICKTRRNsVFQQGMK
0 5 K950 sVFQQGMKNKILIFG
0 4 K1019 RPGGWVEKETYY___
3060 : Phospho-Na,K-ATPase alpha 1 (Tyr10) Antibody
13566 : Phospho-Na,K-ATPase alpha1 (Tyr10) (E1Y9C) Rabbit mAb
4020 : Phospho-Na,K-ATPase alpha1 (Ser16) Antibody
4006 : Phospho-Na,K-ATPase alpha1 (Ser23) Antibody
  human

► Hide Isoforms
 
K9-ub GKGVGRDkyEPAAVs
Y10-p KGVGRDkyEPAAVsE
S16-p kyEPAAVsEQGDkKG
K21-ub AVsEQGDkKGKKGKK
K22 VsEQGDkKGKKGKKD
G23 sEQGDkKGKKGKKDR
K24 EQGDkKGKKGKKDRD
K25 QGDkKGKKGKKDRDM
K36-ub DRDMDELkkEVSMDD
K37-ub RDMDELkkEVSMDDH
S40 DELkkEVSMDDHkLs
K45-ub EVSMDDHkLsLDELH
S47-p SMDDHkLsLDELHRk
K54-ub sLDELHRkyGTDLsR
Y55-p LDELHRkyGTDLsRG
T57 ELHRkyGTDLsRGLT
S60-p RkyGTDLsRGLTSAR
K156-ub YQEAKSSkIMESFkN
K162-ub SkIMESFkNMVPQQA
K177-ub LVIRNGEkMSINAEE
K194 VGDLVEVKGGDRIPA
S207-p PADLRIIsANGCkVD
K212-ub IIsANGCkVDNssLT
S216-p NGCkVDNssLTGEsE
S217-p GCkVDNssLTGEsEP
T219 kVDNssLTGEsEPQt
S222-p NssLTGEsEPQtRsP
T226-p TGEsEPQtRsPDFTN
S228-p EsEPQtRsPDFTNEN
S246-p TRNIAFFsTNCVEGT
Y260-p TARGIVVytGDRtVM
T261-p ARGIVVytGDRtVMG
T265-p VVytGDRtVMGRIAT
S275 GRIATLASGLEGGQT
K359-ub ARKNCLVkNLEAVET
K377 TSTICSDKTGTLTQN
K377-ub TSTICSDkTGTLTQN
S407 ADTTENQSGVSFDKT
K413 QSGVSFDKTSATWLA
K444-ub QENLPILkRAVAGDA
S452 RAVAGDASESALLKC
S454 VAGDASESALLKCIE
K468-ub ELCCGSVkEMRERYA
Y474 VkEMRERYAkIVEIP
K476-ub EMRERYAkIVEIPFN
S484-p IVEIPFNsTNkYQLS
T485 VEIPFNsTNkYQLSI
K487-ac IPFNsTNkYQLSIHk
K487-ub IPFNsTNkYQLSIHk
K494-ub kYQLSIHkNPNtsEP
K494 kYQLSIHKNPNtsEP
T498-p SIHkNPNtsEPQHLL
S499-p IHkNPNtsEPQHLLV
Q502 NPNtsEPQHLLVMkG
Q502 NPNtsEPQHLLVMkG
K508-ub PQHLLVMkGAPERIL
K526 SSILLHGKEQPLDEE
K535-ub QPLDEELkDAFQNAy
K535 QPLDEELKDAFQNAy
Y542-p kDAFQNAyLELGGLG
K605-ub AVPDAVGkCRSAGIK
T617-p GIKVIMVtGDHPITA
K625-ub GDHPITAkAIAkGVG
K629-ub ITAkAIAkGVGIISE
S635 AkGVGIISEGNETVE
- gap
- gap
T640 IISEGNETVEDIAAR
S653-p ARLNIPVsQVNPRDA
K661-ub QVNPRDAkACVVHGS
S668 kACVVHGSDLkDMTS
K671-ub VVHGSDLkDMTSEQL
S675 SDLkDMTSEQLDDIL
K683-ub EQLDDILkyHtEIVF
Y684-p QLDDILkyHtEIVFA
T686-p DDILkyHtEIVFART
K698-ub ARTSPQQkLIIVEGC
S722-p TGDGVNDsPALkkAD
K726 VNDsPALKkADIGVA
K726-ub VNDsPALkkADIGVA
K727 NDsPALkKADIGVAM
K727-ub NDsPALkkADIGVAM
K773-ub RLIFDNLkKSIAYTL
K833-ub QAESDIMkRQPRNPk
K840-ub kRQPRNPkTDkLVNE
K843-ub PRNPkTDkLVNERLI
S943-p ICKTRRNsVFQQGMk
K950-ub sVFQQGMkNKILIFG
K1019-ub RPGGWVEkETYY___
3060 : Phospho-Na,K-ATPase alpha 1 (Tyr10) Antibody
13566 : Phospho-Na,K-ATPase alpha1 (Tyr10) (E1Y9C) Rabbit mAb
  ATP1A1 iso2  
K9 GKGVGRDKYEPAAVS
Y10 KGVGRDKYEPAAVSE
S16 KYEPAAVSEQGDKKG
K21 AVSEQGDKKGKKGKK
K22 VSEQGDKKGKKGKKD
G23 SEQGDKKGKKGKKDR
K24 EQGDKKGKKGKKDRD
K25 QGDKKGKKGKKDRDM
K36 DRDMDELKKEVSMDD
K37 RDMDELKKEVSMDDH
S40 DELKKEVSMDDHKLS
K45 EVSMDDHKLSLDELH
S47 SMDDHKLSLDELHRK
K54 SLDELHRKYGTDLSR
Y55 LDELHRKYGTDLSRG
T57 ELHRKYGTDLSRGLT
S60 RKYGTDLSRGLTSAR
K156 YQEAKSSKIMESFKN
K162 SKIMESFKNMVPQQA
K177 LVIRNGEKMSINAEE
K194 VGDLVEVKGGDRIPA
S207 PADLRIISANGCKVD
K212 IISANGCKVDNSSLT
S216 NGCKVDNSSLTGESE
S217 GCKVDNSSLTGESEP
T219 KVDNSSLTGESEPQT
S222 NSSLTGESEPQTRSP
T226 TGESEPQTRSPDFTN
S228 ESEPQTRSPDFTNEN
S246 TRNIAFFSTNCVEGT
Y260 TARGIVVYTGDRTVM
T261 ARGIVVYTGDRTVMG
T265 VVYTGDRTVMGRIAT
S275 GRIATLASGLEGGQT
K359 ARKNCLVKNLEAVET
K377 TSTICSDKTGTLTQN
K377 TSTICSDKTGTLTQN
S407 ADTTENQSGVSFDKT
K413 QSGVSFDKTSATWLA
K444 QENLPILKRAVAGDA
S452 RAVAGDASESALLKC
S454 VAGDASESALLKCIE
K468 ELCCGSVKEMRERYA
Y474 VKEMRERYAKIVEIP
K476 EMRERYAKIVEIPFN
S484 IVEIPFNSTNKYQLS
T485 VEIPFNSTNKYQLSI
K487 IPFNSTNKYQLSIHK
K487 IPFNSTNKYQLSIHK
K494 KYQLSIHKNPNTSEP
K494 KYQLSIHKNPNTSEP
T498 SIHKNPNTSEPQHLL
S499 IHKNPNTSEPQHLLV
Q502 NPNTSEPQHLLVMKG
Q502 NPNTSEPQHLLVMKG
K508 PQHLLVMKGAPERIL
K526 SSILLHGKEQPLDEE
K535 QPLDEELKDAFQNAY
K535 QPLDEELKDAFQNAY
Y542 KDAFQNAYLELGGLG
K605 AVPDAVGKCRSAGIK
T617 GIKVIMVTGDHPITA
K625 GDHPITAKAIAKGVG
K629 ITAKAIAKGVGIIsE
S635-p AKGVGIIsEGsGPMs
S638-p VGIIsEGsGPMsRGK
S642-p sEGsGPMsRGKSWSS
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
K9-ub GKGVGRDkyEPAAVs
Y10-p KGVGRDkyEPAAVsE
S16-p kyEPAAVsEHGDkkG
K21-ub AVsEHGDkkGKKAKK
K22-ub VsEHGDkkGKKAKKE
G23 sEHGDkkGKKAKKER
K24 EHGDkkGKKAKKERD
K25 HGDkkGKKAKKERDM
K36 ERDMDELKkEVsMDD
K37-ub RDMDELKkEVsMDDH
S40-p DELKkEVsMDDHkLs
K45-ub EVsMDDHkLsLDELH
S47-p sMDDHkLsLDELHRk
K54-ub sLDELHRkyGtDLSR
Y55-p LDELHRkyGtDLSRG
T57-p ELHRkyGtDLSRGLT
S60 RkyGtDLSRGLTPAR
K156-ub YQEAKSSkIMESFkN
K162-ub SkIMESFkNMVPQQA
K177-ub LVIRNGEkMSINAED
K194-ub VGDLVEVkGGDRIPA
S207-p PADLRIIsANGCkVD
K212-ub IIsANGCkVDNSsLt
S216 NGCkVDNSsLtGEsE
S217-p GCkVDNSsLtGEsEP
T219-p kVDNSsLtGEsEPQt
S222-p NSsLtGEsEPQtRsP
T226-p tGEsEPQtRsPDFTN
S228-p EsEPQtRsPDFTNEN
S246 TRNIAFFSTNCVEGT
Y260-p TARGIVVytGDRtVM
T261-p ARGIVVytGDRtVMG
T265-p VVytGDRtVMGRIAT
S275-p GRIATLAsGLEGGQT
K359-ub ARKNCLVkNLEAVET
K377-ac TSTICSDkTGTLTQN
K377-ub TSTICSDkTGTLTQN
S407-p ADTTENQsGVSFDkT
K413-ub QsGVSFDkTSATWFA
K444-ub QENLPILkRAVAGDA
S452-p RAVAGDAsEsALLKC
S454-p VAGDAsEsALLKCIE
M468 EVCCGSVMEMREKYS
Y474 VMEMREKYSkIVEIP
K476-ub EMREKYSkIVEIPFN
S484-p IVEIPFNstNkYQLS
T485-p VEIPFNstNkYQLSI
K487-ac IPFNstNkYQLSIHk
K487-ub IPFNstNkYQLSIHk
K494-ub kYQLSIHkNPNAsEP
K494-ac kYQLSIHkNPNAsEP
A498 SIHkNPNAsEPkHLL
S499-p IHkNPNAsEPkHLLV
K502-ac NPNAsEPkHLLVMkG
K502-ub NPNAsEPkHLLVMkG
K508-ub PkHLLVMkGAPERIL
K526-ub SSILLHGkEQPLDEE
K535 QPLDEELKDAFQNAy
K535-ac QPLDEELkDAFQNAy
Y542-p kDAFQNAyLELGGLG
K605-ub AVPDAVGkCRSAGIK
T617-p GIKVIMVtGDHPITA
K625-ub GDHPITAkAIAkGVG
K629-ub ITAkAIAkGVGIIsE
S635-p AkGVGIIsEGNEtVE
- gap
- gap
T640-p IIsEGNEtVEDIAAR
N653 ARLNIPVNQVNPRDA
K661-ub QVNPRDAkACVVHGs
S668-p kACVVHGsDLkDMTs
K671-ub VVHGsDLkDMTsEEL
S675-p sDLkDMTsEELDDIL
R683 EELDDILRyHTEIVF
Y684-p ELDDILRyHTEIVFA
T686 DDILRyHTEIVFART
K698-ub ARTSPQQkLIIVEGC
S722-p TGDGVNDsPALkkAD
K726-ac VNDsPALkkADIGVA
K726-ub VNDsPALkkADIGVA
K727 NDsPALkKADIGVAM
K727-ub NDsPALkkADIGVAM
K773-ub RLIFDNLkKSIAYTL
K833-ub QAESDIMkRQPRNPk
K840-ub kRQPRNPkTDkLVNE
K843-ub PRNPkTDkLVNERLI
S943-p ICKTRRNsVFQQGMk
K950-ub sVFQQGMkNKILIFG
K1019-ub RPGGWVEkETYY___
3060 : Phospho-Na,K-ATPase alpha 1 (Tyr10) Antibody
13566 : Phospho-Na,K-ATPase alpha1 (Tyr10) (E1Y9C) Rabbit mAb
4020 : Phospho-Na,K-ATPase alpha1 (Ser16) Antibody
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