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Protein Page:
CAF-1A (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
CAF-1A Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci. The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D- coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR- mediated transrepression of the CYP27B1 gene. Homodimer. Part of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1A binds directly to CHAF1B. Only minor amounts of RBBP4 are complexed with CHAF1A and CHAF1B in G1 phase. Part of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. CHAF1A binds directly to PCNA and to CBX1. Binds MBD1. Interacts directly with CBX5 via the PxVxL motif. During DNA replication, it forms a S phase- specific complex that facilitates DNA methylation and histone H3 'Lys-9' methylation during replication-coupled chromatin assembly and is at least composed of the CHAF1A, MBD1 and SETDB1. Component of the WINAC complex, at least composed of SMARCA2, SMARCA4, SMARCB1, SMARCC1, SMARCC2, SMARCD1, SMARCE1, ACTL6A, BAZ1B/WSTF, ARID1A, SUPT16H, CHAF1A and TOP2B. Interacts with CBX5. Belongs to the CHAF1A family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: DNA replication
Molecular Function: identical protein binding; protein binding; unfolded protein binding; chromatin binding
Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; chromatin assembly; protein complex assembly; DNA replication; cell cycle; DNA repair; DNA replication-dependent nucleosome assembly
Reference #:  Q13111 (UniProtKB)
Alt. Names/Synonyms: CAF; CAF-1; CAF-1 subunit A; CAF-I 150 kDa subunit; CAF-I p150; CAF1; CAF1A; CAF1B; CAF1P150; CHAF1A; Chromatin assembly factor 1 subunit A; chromatin assembly factor 1, subunit A (p150); chromatin assembly factor I (150 kDa); Chromatin assembly factor I p150 subunit; hp150; MGC71229; P150
Gene Symbols: CHAF1A
Molecular weight: 106,926 Da
Basal Isoelectric point: 5.69  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

CAF-1A

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 4 S65-p GTSVQSKsPDLEASL
0 1 T74-p DLEASLDtLENNCHV
0 1 T114-p ETSIGQStVIIDLTE
0 3 S123-p IIDLTEDsNEQPdSL
0 1 D128-ca EDsNEQPdSLVDHNK
0 1 S129 DsNEQPdSLVDHNKL
0 1 S138-p VDHNKLNsEAsPsRE
0 6 S141-p NKLNsEAsPsREAIN
0 2 S143-p LNsEAsPsREAINGQ
0 2 T154-p INGQREDtGDQQGLL
0 3 S199-p GAGRRGDsQECsPRs
0 6 S203-p RGDsQECsPRsCPEL
0 13 S206-p sQECsPRsCPELTSG
0 2 S224-p CPRKEQDsWSEAGGI
0 7 S255-p VRPPQIKsLPAtPQG
0 10 T259-p QIKsLPAtPQGKNMT
0 3 S308-p APPKQHSstsPFPts
0 9 T309-p PPKQHSstsPFPtst
0 10 S310-p PKQHSstsPFPtstP
0 1 T314-p SstsPFPtstPLRRI
0 5 S315-p stsPFPtstPLRRIT
0 8 T316-p tsPFPtstPLRRITK
0 1 T330-p KKFVKGStEKNKLRL
0 3 T454-p RFFQKPKtPQAPKTL
0 3 K467-a TLAGSCGkFAPFEIK
0 3 T722-p LPAQEEQtPKASKRE
0 1 S767-p HCRRGLLsNHtGsPR
0 3 T770-p RGLLsNHtGsPRsPs
0 15 S772-p LLsNHtGsPRsPstt
0 24 S775-p NHtGsPRsPsttyLH
0 8 S777-p tGsPRsPsttyLHtP
0 3 T778-p GsPRsPsttyLHtPT
0 3 T779-p sPRsPsttyLHtPTP
0 3 Y780-p PRsPsttyLHtPTPS
0 9 T783-p PsttyLHtPTPSEDA
0 1 S803-p SRLKRLIsENSVYEK
0 2 S862-p SVPSTGPsQGtPIsL
0 6 T865-p STGPsQGtPIsLKRK
0 2 S868-p PsQGtPIsLKRKsAG
0 5 S873-p PIsLKRKsAGsMCIT
0 1 S876-p LKRKsAGsMCITQFM
0 1 T947-p GVDTGKAtLtssPLG
0 1 T949-p DTGKAtLtssPLGAS
0 1 S950-p TGKAtLtssPLGAS_
0 7 S951-p GKAtLtssPLGAS__
  mouse

 
V60 KAAVESKVPDLQLSL
T69 DLQLSLGTFESQCHT
V107 TIKPVPSVVIIDLTE
C116 IIDLTENCSDIPDsP
D121 ENCSDIPDsPEGHSE
S122-p NCSDIPDsPEGHSEL
S127 PDsPEGHSELSPDTA
S130 PEGHSELSPDTAGVV
D132 GHSELSPDTAGVVTT
A143 VVTTVEGAAKQQEHS
C183 DPKRTGDCQAGSLQS
S187 TGDCQAGSLQSCPEL
S190 CQAGSLQSCPELTPG
S208 CPTKELSSWSKAGDL
S238 ATKPSIASLPMMSLD
M242 SIASLPMMSLDRSVT
G291 TPPEHRGGRssPSTP
R292 PPEHRGGRssPSTPA
S293-p PEHRGGRssPSTPAC
S294-p EHRGGRssPSTPACR
S296 RGGRssPSTPACRVA
T297 GGRssPSTPACRVAK
T311 KNFVKGSTEKGRSKL
T433 RFFQKPKTPQAPKTL
K446 TLAGSCGKFAPFEIK
E701 VASPDEPEPGASRRE
T746 QCRRGLLTLPSPtPH
S749 RGLLTLPSPtPHLQM
T751-p LLTLPSPtPHLQMPN
- gap
- gap
- gap
- gap
H753 TLPSPtPHLQMPNLE
M756 SPtPHLQMPNLEDAV
S776-p ARLKRLIsENSAYEK
G835 SASTEGPGQSTPMLL
T838 TEGPGQSTPMLLKRK
L841 PGQSTPMLLKRKPAA
P846 PMLLKRKPAATMCIT
T849 LKRKPAATMCITQFM
- gap
- gap
- gap
- gap
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