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Protein Page:
LST8 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
LST8 Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. Growth factor-stimulated mTORC1 activation involves a AKT1- mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-389', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity. In nutrient- poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum- induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Part of the mammalian target of rapamycin complex 1 (mTORC1) which contains MTOR, MLST8, RPTOR, AKT1S1/PRAS40 and DEPTOR. mTORC1 binds to and is inhibited by FKBP12-rapamycin. Part of the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12- rapamycin. Interacts directly with MTOR and RPTOR. Interacts with RHEB. Broadly expressed, with highest levels in skeletal muscle, heart and kidney. Belongs to the WD repeat LST8 family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Autophagy
Cellular Component: cytoplasm; cytosol
Molecular Function: protein binding
Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of actin filament polymerization; regulation of actin cytoskeleton organization and biogenesis; T cell costimulation; insulin receptor signaling pathway; innate immune response; regulation of Rac GTPase activity; positive regulation of TOR signaling pathway
Reference #:  Q9BVC4 (UniProtKB)
Alt. Names/Synonyms: G protein beta subunit like; G protein beta subunit-like; Gable; GbetaL; GBL; LST8; Mammalian lethal with SEC13 protein 8; MGC111011; MLST8; MTOR associated protein, LST8 homolog (S. cerevisiae); POP3; Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit LST8; WAT1
Gene Symbols: MLST8
Molecular weight: 35,876 Da
Basal Isoelectric point: 5.5  Predict pI for various phosphorylation states
CST Pathways:  AMPK Signaling  |  Autophagy Signaling  |  Insulin Receptor Signaling  |  mTOR Signaling  |  PI3K/Akt Signaling  |  T Cell Receptor Signaling  |  Translation: eIF4E and p70S6K
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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LST8

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 - gap
0 2 S4-p ____MNTsPGTVGSD
0 4 S43-p RTVQHQDsQVNALEV
0 1 S55-p LEVTPDRsMIAAAGy
0 1 Y62-p sMIAAAGyQHIRMYD
0 1 K86-ub ISYDGVNkNIASVGF
0 1 T214-p VTQLIPKtKIPAHTR
0 1 Y222-p KIPAHTRyALQCRFS
0 1 G263 TELSIKSGNPGESSR
  LST8 iso4  
S7-p _MEHAPWsPGASSRA
S23 AGHTMNTSPGTVGSD
S62 RTVQHQDSQVNALEV
S74 LEVTPDRSMIAAAGY
Y81 SMIAAAGYQHIRMYD
K105 ISYDGVNKNIASVGF
- gap
- gap
- gap
  mouse

 
- gap
T4 ____MNTTPGTVGSD
S43 RTVQHQDSQVNALEI
S55 LEITPDRSMIAAAGY
Y62 SMIAAAGYQHIRMYD
K86 ISYDGVSKNIASVGF
T214 VTQLIPKTKIPAHTR
Y222 KIPAHTRYALQCRFS
S263-p TELSIKSsNPGESSR
  rat

 
- gap
T4 ____MNTTPGTVGSD
S43 RTVQHQDSQVNALEI
S55 LEITPDRSMIAATGY
Y62 SMIAATGYQHIRMYD
K86 ISYDGVSKNIASVGF
T214 VTQLIPKTKIPAHTR
Y222 KIPAHTRYALQCRFS
S263 TELSIKSSNPGESSR
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