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Protein Page:
GIT2 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GIT2 possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Interacts with G protein-coupled receptor kinases. Associates with paxillin. Also interacts with PIX exchange factors. Regulates Golgi and actin cytoskeletal organization. Important in integrin-mediated cell adhesion. Ten alternatively spliced isoforms have been described and additional isoforms seem to exist. Contains 1 Arf-GAP domain and 3 ANK repeats. Note: This description may include information from UniProtKB.
Protein type: GAPs, ARF; GAPs; Motility/polarity/chemotaxis
Cellular Component: nucleoplasm
Molecular Function: protein binding; ARF GTPase activator activity; zinc ion binding
Biological Process: regulation of ARF GTPase activity; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity
Reference #:  Q14161 (UniProtKB)
Alt. Names/Synonyms: ARF GAP GIT2; ARF GTPase-activating protein GIT2; CAT-2; CAT2; cool-associated, tyrosine phosphorylated protein 2; Cool-interacting tyrosine-phosphorylated protein 2; DKFZp686G01261; G protein-coupled receptor kinase interacting ArfGAP 2; G protein-coupled receptor kinase interactor 2; G protein-coupled receptor kinase-interactor 2; GIT2; GRK-interacting protein 2; KIAA0148; MGC760
Gene Symbols: GIT2
Molecular weight: 84,543 Da
Basal Isoelectric point: 6.78  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GIT2

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 4 S7-p _MSKRLRssEVCADC
0 1 S8-p MSKRLRssEVCADCS
0 2 S256 IPQMADSSLDLSELA
0 1 S260 ADSSLDLSELAKAAK
1 0 Y286 EELAMDVYDEVDRRE
0 1 T344 FNAHEFATLVIDILS
0 4 S360 AKRRQQGSSLSGsKD
0 1 S365-p QGSSLSGsKDNVELI
0 1 V382 TINNQHSVESQDNDQ
0 2 S384 NNQHSVESQDNDQPd
0 1 D391-ca SQDNDQPdyDsVAsD
1 29 Y392-p QDNDQPdyDsVAsDE
0 55 S394-p NDQPdyDsVAsDEDt
0 72 S397-p PdyDsVAsDEDtDLE
0 13 T401-p sVAsDEDtDLEttAs
0 1 T405-p DEDtDLEttAsKTNR
0 1 T406-p EDtDLEttAsKTNRQ
0 1 S408-p tDLEttAsKTNRQKs
0 10 S415-p sKTNRQKsLDsDLsD
0 5 S418-p NRQKsLDsDLsDGPV
0 10 S421-p KsLDsDLsDGPVTVQ
0 1 T480-p SNLRKQAttNVyQVQ
0 2 T481-p NLRKQAttNVyQVQt
0 202 Y484-p KQAttNVyQVQtGsE
0 9 T488-p tNVyQVQtGsEytDt
0 16 S490-p VyQVQtGsEytDtsN
0 158 Y492-p QVQtGsEytDtsNHS
0 5 T493-p VQtGsEytDtsNHSs
0 2 T495-p tGsEytDtsNHSsLK
0 3 S496-p GsEytDtsNHSsLKR
0 18 S500-p tDtsNHSsLKRRPsA
0 17 S506-p SsLKRRPsARGSRPM
0 2 S510 RRPsARGSRPMsMyE
0 7 S514-p ARGSRPMsMyEtGSG
0 5 Y516-p GSRPMsMyEtGSGQK
0 1 T518-p RPMsMyEtGSGQKPy
0 2 Y525-p tGSGQKPyLPMGEAs
0 1 S532-p yLPMGEAsRPEESRM
0 1 T555 IGRSALVTSsssLPs
0 1 S556 GRSALVTSsssLPsF
0 2 S557-p RSALVTSsssLPsFP
0 2 S558-p SALVTSsssLPsFPs
0 15 S559-p ALVTSsssLPsFPsT
0 2 S562-p TSsssLPsFPsTLsW
0 2 S565-p ssLPsFPsTLsWsRD
0 6 S568-p PsFPsTLsWsRDESA
0 13 S570-p FPsTLsWsRDESARR
0 5 S579 DESARRASRLEKQNs
0 16 S586-p SRLEKQNstPEsDyD
0 5 T587-p RLEKQNstPEsDyDN
0 25 S590-p KQNstPEsDyDNTPN
1 233 Y592-p NstPEsDyDNTPNDM
0 29 S614-p SRKGRQRsMVWPGDG
0 1 S634 AEPHVAPSPTLPSTE
0 1 T636 PHVAPSPTLPSTEDV
0 1 T647 TEDVIRKTEQITKNI
0 1 S668-p AQENKHDsYIPCsER
0 1 S673-p HDsYIPCsERIHVAV
0 1 S693-p LFPKKPKsDMVRtsL
0 1 T698-p PKsDMVRtsLRLLTS
0 1 S699-p KsDMVRtsLRLLTSS
11873 : Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
13167 : Phospho-GIT2 (Tyr592) (D6L1J) Rabbit mAb
  GIT2 iso10  
S7 _MSKRLRSSEVCADC
S8 MSKRLRSSEVCADCS
S258 QMADRRLSLDLsELA
S262-p RRLSLDLsELAKAAK
Y288 EELAMDVYDEVDRRE
T346 FNAHEFATLVIDILS
S362 AKRRQQGSSLSGSKD
S367 QGSSLSGSKDNVELI
V384 TINNQHSVESQDNDQ
S386 NNQHSVESQDNDQPD
D393 SQDNDQPDYDSVASD
Y394 QDNDQPDYDSVASDE
S396 NDQPDYDSVASDEDT
S399 PDYDSVASDEDTDLE
T403 SVASDEDTDLETTAS
T407 DEDTDLETTASKTNR
T408 EDTDLETTASKTNRQ
S410 TDLETTASKTNRQKL
- gap
- gap
- gap
T432 SNLRKQATTNVYQVQ
T433 NLRKQATTNVYQVQT
Y436 KQATTNVYQVQTGSE
T440 TNVYQVQTGSEYTDT
S442 VYQVQTGSEYTDTSN
Y444 QVQTGSEYTDTSNHS
T445 VQTGSEYTDTSNHSS
T447 TGSEYTDTSNHSSLK
S448 GSEYTDTSNHSSLKR
S452 TDTSNHSSLKRRPSA
S458 SSLKRRPSARGSRPM
S462 RRPSARGSRPMSMYE
S466 ARGSRPMSMYETGSG
Y468 GSRPMSMYETGSGQK
T470 RPMSMYETGSGQKPY
Y477 TGSGQKPYLPMGEAS
S484 YLPMGEASRPEESRM
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S501 QPFPAHASRLEKQNS
S508 SRLEKQNSTPESDYD
T509 RLEKQNSTPESDYDN
S512 KQNSTPESDYDNTPN
Y514 NSTPESDYDNTPNDM
S536 SRKGRQRSMVWPGDG
S556 AEPHVAPSPTLPSTE
T558 PHVAPSPTLPSTEDV
T569 TEDVIRKTEQITKNI
S590 AQENKHDSYIPCSER
S595 HDSYIPCSERIHVAV
S615 LFPKKPKSDMVRTSL
T620 PKSDMVRTSLRLLTS
S621 KSDMVRTSLRLLTSS
11873 : Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
  mouse

► Hide Isoforms
 
S7-p _MSKRLRsSDVCADC
S8 MSKRLRsSDVCADCN
S255 LIPQRADSLDLSELA
S259 RADSLDLSELAKAAK
Y285 EELAMDVYDEVDRRE
T343-p FNAHEFAtLVIDILS
S359-p AKRRQQGsPLSRSKD
S364 QGsPLSRSKDNVELI
T381-p TVSTQHStEsQDNDQ
S383-p STQHStEsQDNDQPD
D390 sQDNDQPDyDsVAsD
Y391-p QDNDQPDyDsVAsDE
S393-p NDQPDyDsVAsDEDt
S396-p PDyDsVAsDEDtDVE
T400-p sVAsDEDtDVETRAS
T404 DEDtDVETRASKANR
R405 EDtDVETRASKANRQ
S407 tDVETRASKANRQKL
- gap
- gap
- gap
T429 SSLRRQATASACQVQ
A430 SLRRQATASACQVQT
C433 RQATASACQVQTGSD
T437 ASACQVQTGSDHKDT
S439 ACQVQTGSDHKDTAS
H441 QVQTGSDHKDTASHS
K442 VQTGSDHKDTASHSs
T444 TGSDHKDTASHSsLK
A445 GSDHKDTASHSsLKR
S449-p KDTASHSsLKRRPsA
S455-p SsLKRRPsARGsRPM
S459-p RRPsARGsRPMsMyE
S463-p ARGsRPMsMyETGSG
Y465-p GsRPMsMyETGSGQK
T467 RPMsMyETGSGQKPY
Y474 TGSGQKPYLPMGEAS
S481 YLPMGEASHPEESRT
T504-p IGRSALVtssssLPs
S505-p GRSALVtssssLPsF
S506-p RSALVtssssLPsFP
S507-p SALVtssssLPsFPS
S508-p ALVtssssLPsFPST
S511-p tssssLPsFPSTLsW
S514 ssLPsFPSTLsWsRD
S517-p PsFPSTLsWsRDESA
S519-p FPSTLsWsRDESARR
S528-p DESARRAsRLEKQNs
S535-p sRLEKQNstPEsDyD
T536-p RLEKQNstPEsDyDN
S539-p KQNstPEsDyDNTAC
Y541-p NstPEsDyDNTACDP
S563-p TRKGRQRsMLWQGDG
S583-p AEPHSVPsPtLPSTE
T585-p PHSVPsPtLPSTEDV
T596-p TEDVIRKtEQITKNI
S617 AQENKHDSYIPCSER
S622 HDSYIPCSERIHVAV
S642 LFPKKPKSDTVRTSL
T647 PKSDTVRTSLRLLTS
S648 KSDTVRTSLRLLTSS
11873 : Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
  GIT2 iso3  
S7 _MSKRLRSSDVCADC
S8 MSKRLRSSDVCADCN
S256-p IPQRADSsLDLSELA
S260 ADSsLDLSELAKAAK
Y286 EELAMDVYDEVDRRE
T344 FNAHEFATLVIDILS
S360 AKRRQQGSPLSRSKD
S365 QGSPLSRSKDNVELI
T382 TVSTQHSTESQDNDQ
S384 STQHSTESQDNDQPD
D391 SQDNDQPDYDSVASD
Y392 QDNDQPDYDSVASDE
S394 NDQPDYDSVASDEDT
S397 PDYDSVASDEDTDVE
T401 SVASDEDTDVETRAS
T405 DEDTDVETRASKANR
R406 EDTDVETRASKANRQ
S408 TDVETRASKANRQKL
- gap
- gap
- gap
T430 SSLRRQATASACQVQ
A431 SLRRQATASACQVQT
C434 RQATASACQVQTGSD
T438 ASACQVQTGSDHKDT
S440 ACQVQTGSDHKDTAS
H442 QVQTGSDHKDTASHS
K443 VQTGSDHKDTASHSS
T445 TGSDHKDTASHSSLK
A446 GSDHKDTASHSSLKR
S450 KDTASHSSLKRRPSA
S456 SSLKRRPSARGSRPM
S460 RRPSARGSRPMSMYE
S464 ARGSRPMSMYETGSG
Y466 GSRPMSMYETGSGQK
T468 RPMSMYETGSGQKPY
Y475 TGSGQKPYLPMGEAS
S482 YLPMGEASHPEESRT
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S499 QPFPTHASRLEKQNS
S506 SRLEKQNSTPESDYD
T507 RLEKQNSTPESDYDN
S510 KQNSTPESDYDNTAC
Y512 NSTPESDYDNTACDP
S534 TRKGRQRSMLWQGDG
S554 AEPHSVPSPTLPSTE
T556 PHSVPSPTLPSTEDV
T567 TEDVIRKTEQITKNI
S588 AQENKHDSYIPCSER
S593 HDSYIPCSERIHVAV
S613 LFPKKPKSDTVRTSL
T618 PKSDTVRTSLRLLTS
S619 KSDTVRTSLRLLTSS
11873 : Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
  GIT2 iso4  
S7 _MSKRLRSSDVCADC
S8 MSKRLRSSDVCADCN
S256-p IPQRADSsLDLSELA
S260 ADSsLDLSELAKAAK
Y286 EELAMDVYDEVDRRE
T344 FNAHEFATLVIDILS
S360 AKRRQQGSPLSRSKD
S365 QGSPLSRSKDNVELI
T382 TVSTQHSTESQDNDQ
S384 STQHSTESQDNDQPD
D391 SQDNDQPDYDSVASD
Y392 QDNDQPDYDSVASDE
S394 NDQPDYDSVASDEDT
S397 PDYDSVASDEDTDVE
T401 SVASDEDTDVETRAS
T405 DEDTDVETRASKANR
R406 EDTDVETRASKANRQ
S408 TDVETRASKANRQKs
S415-p SKANRQKsLDsDLsD
S418-p NRQKsLDsDLsDGPV
S421-p KsLDsDLsDGPVTVQ
T480 SSLRRQATASACQVQ
A481 SLRRQATASACQVQT
C484 RQATASACQVQTGSD
T488 ASACQVQTGSDHKDT
S490 ACQVQTGSDHKDTAS
H492 QVQTGSDHKDTASHS
K493 VQTGSDHKDTASHSS
T495 TGSDHKDTASHSSLK
A496 GSDHKDTASHSSLKR
S500 KDTASHSSLKRRPSA
S506 SSLKRRPSARGSRPM
S510 RRPSARGSRPMSMYE
S514 ARGSRPMSMYETGSG
Y516 GSRPMSMYETGSGQK
T518 RPMSMYETGSGQKPY
Y525 TGSGQKPYLPMGEAS
S532 YLPMGEASHPEESRT
T555 IGRSALVTSSSSLPS
S556 GRSALVTSSSSLPSF
S557 RSALVTSSSSLPSFP
S558 SALVTSSSSLPSFPS
S559 ALVTSSSSLPSFPST
S562 TSSSSLPSFPSTLSW
S565 SSLPSFPSTLSWSRD
S568 PSFPSTLSWSRDESA
S570 FPSTLSWSRDESARR
S579 DESARRASRLEKQNS
S586 SRLEKQNSTPESDYD
T587 RLEKQNSTPESDYDN
S590 KQNSTPESDYDNTAC
Y592 NSTPESDYDNTACDP
S614 TRKGRQRSMLWQGDG
S634 AEPHSVPSPTLPSTE
T636 PHSVPSPTLPSTEDV
T647 TEDVIRKTEQITKNI
S668 AQENKHDSYIPCSER
S673 HDSYIPCSERIHVAV
S693 LFPKKPKSDTVRTSL
T698 PKSDTVRTSLRLLTS
S699 KSDTVRTSLRLLTSS
11873 : Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
  GIT2 iso5  
S7 _MSKRLRSSDVCADC
S8 MSKRLRSSDVCADCN
S258-p QRADRRLsLDLSELA
S262 RRLsLDLSELAKAAK
Y288 EELAMDVYDEVDRRE
T346 FNAHEFATLVIDILS
S362 AKRRQQGSPLSRSKD
S367 QGSPLSRSKDNVELI
T384 TVSTQHSTESQDNDQ
S386 STQHSTESQDNDQPD
D393 SQDNDQPDYDSVASD
Y394 QDNDQPDYDSVASDE
S396 NDQPDYDSVASDEDT
S399 PDYDSVASDEDTDVE
T403 SVASDEDTDVETRAS
T407 DEDTDVETRASKANR
R408 EDTDVETRASKANRQ
S410 TDVETRASKANRQKL
- gap
- gap
- gap
T432 SSLRRQATASACQVQ
A433 SLRRQATASACQVQT
C436 RQATASACQVQTGSD
T440 ASACQVQTGSDHKDT
S442 ACQVQTGSDHKDTAS
H444 QVQTGSDHKDTASHS
K445 VQTGSDHKDTASHSS
T447 TGSDHKDTASHSSLK
A448 GSDHKDTASHSSLKR
S452 KDTASHSSLKRRPSA
S458 SSLKRRPSARGSRPM
S462 RRPSARGSRPMSMYE
S466 ARGSRPMSMYETGSG
Y468 GSRPMSMYETGSGQK
T470 RPMSMYETGSGQKPY
Y477 TGSGQKPYLPMGEAS
S484 YLPMGEASHPEESRT
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S501 QPFPTHASRLEKQNS
S508 SRLEKQNSTPESDYD
T509 RLEKQNSTPESDYDN
S512 KQNSTPESDYDNTAC
Y514 NSTPESDYDNTACDP
S536 TRKGRQRSMLWQGDG
S556 AEPHSVPSPTLPSTE
T558 PHSVPSPTLPSTEDV
T569 TEDVIRKTEQITKNI
S590 AQENKHDSYIPCSER
S595 HDSYIPCSERIHVAV
S615 LFPKKPKSDTVRTSL
T620 PKSDTVRTSLRLLTS
S621 KSDTVRTSLRLLTSS
11873 : Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
  rat

 
S7 _MSKRLRSNDVCADC
N8 MSKRLRSNDVCADCS
S256 IPQMADSSLDLSELA
S260 ADSSLDLSELAKAAK
Y286 EELAMDVYDEVDRRE
T344 FNAHEFATLVIDILS
S360-p AKRRQQGsPLSRSKD
S365 QGsPLSRSKDNVELI
T382 TVSNQHSTESQDNDQ
S384 SNQHSTESQDNDQPD
D391 SQDNDQPDYDsVAsD
Y392 QDNDQPDYDsVAsDE
S394-p NDQPDYDsVAsDEDt
S397-p PDYDsVAsDEDtDVE
T401-p sVAsDEDtDVETRAS
T405 DEDtDVETRASRTNR
R406 EDtDVETRASRTNRQ
S408 tDVETRASRTNRQKs
S415-p SRTNRQKsLDsDLsD
S418-p NRQKsLDsDLsDGPV
S421-p KsLDsDLsDGPVTVQ
T480 SSLRRQATASACQVQ
A481 SLRRQATASACQVQT
C484 RQATASACQVQTASD
T488 ASACQVQTASDHKDT
S490 ACQVQTASDHKDTVS
H492 QVQTASDHKDTVSHS
K493 VQTASDHKDTVSHSS
T495 TASDHKDTVSHSSLK
V496 ASDHKDTVSHSSLKR
S500 KDTVSHSSLKRRPSA
S506 SSLKRRPSARGSRPM
S510 RRPSARGSRPMSMYE
S514 ARGSRPMSMYETGSG
Y516 GSRPMSMYETGSGQK
T518 RPMSMYETGSGQKPY
Y525 TGSGQKPYLPMGEAN
N532 YLPMGEANHPEESRT
T555 IGRSALVTSSSSLPS
S556 GRSALVTSSSSLPSF
S557 RSALVTSSSSLPSFP
S558 SALVTSSSSLPSFPS
S559 ALVTSSSSLPSFPST
S562 TSSSSLPSFPSTLSW
S565 SSLPSFPSTLSWSRD
S568 PSFPSTLSWSRDEST
S570 FPSTLSWSRDESTRR
S579-p DESTRRAsRLEKQNS
S586 sRLEKQNSTPESDYD
T587 RLEKQNSTPESDYDN
S590 KQNSTPESDYDNTAY
Y592 NSTPESDYDNTAYDP
S614-p GRKGRQRsMLWQGDG
S634 AEPHAVPSPALPSTE
A636 PHAVPSPALPSTEDV
T647 TEDVIRKTEQITKNI
S668 AQENKHDSYIPCSER
S673 HDSYIPCSERIHAAV
S693 LFPKKPKSDTVRTSL
T698 PKSDTVRTSLRLLTA
S699 KSDTVRTSLRLLTAS
11873 : Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
  chicken

 
S7 _MSKRLRSSEVCADC
S8 MSKRLRSSEVCADCS
S256 IPQMADSSLDLSELA
S260 ADSSLDLSELAKAAK
Y286-p EELAMYVyDEVDRRE
T344 FNAHEFATLVIDILS
N360 AKRRQQGNPLSGSKE
S365 QGNPLSGSKENVELI
S382 SISNQHSSESQDNDQ
S384 SNQHSSESQDNDQPD
D391 SQDNDQPDyDSVASD
Y392-p QDNDQPDyDSVASDE
S394 NDQPDyDSVASDEDT
S397 PDyDSVASDEDTDLE
T401 SVASDEDTDLETNAA
T405 DEDTDLETNAAKSTR
N406 EDTDLETNAAKSTRQ
A408 TDLETNAAKSTRQKS
S415 AKSTRQKSLDSDLSD
S418 TRQKSLDSDLSDGPV
S421 KSLDSDLSDGPVTAQ
T480 TNLRRQATTNIYQVQ
T481 NLRRQATTNIYQVQT
Y484 RQATTNIYQVQTGSE
T488 TNIYQVQTGSEYTDP
S490 IYQVQTGSEYTDPTN
Y492 QVQTGSEYTDPTNNS
T493 VQTGSEYTDPTNNSS
P495 TGSEYTDPTNNSSLK
T496 GSEYTDPTNNSSLKR
S500 TDPTNNSSLKRRPSA
S506 SSLKRRPSARGSRPM
S510 RRPSARGSRPMSMYE
S514 ARGSRPMSMYETGSG
Y516 GSRPMSMYETGSGQK
T518 RPMSMYETGSGQKPY
Y525 TGSGQKPYLPMGEVT
T532 YLPMGEVTYPEESIT
T555 IGRSAFVTSSSSLPS
S556 GRSAFVTSSSSLPSF
S557 RSAFVTSSSSLPSFP
S558 SAFVTSSSSLPSFPS
S559 AFVTSSSSLPSFPST
S562 TSSSSLPSFPSTLSW
S565 SSLPSFPSTLSWSRD
S568 PSFPSTLSWSRDEST
S570 FPSTLSWSRDESTRR
S579 DESTRRASKLEKQSS
S586 SKLEKQSSVPESDyD
V587 KLEKQSSVPESDyDN
S590 KQSSVPESDyDNTTA
Y592-p SSVPESDyDNTTASL
S614 GRKGRQRSVIWQGEG
S632 EDTDTAPSSSLPSTE
S634 TDTAPSSSLPSTEDV
T645 TEDVIRKTEQITKNI
S666 AQENKHDSYIPCSER
S671 HDSYIPCSERIHVAV
S691 LFPKKPKSELVRTSL
T696 PKSELVRTSLRLLTS
S697 KSELVRTSLRLLTSS
11873 : Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb
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