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Protein Page:
GIT1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GIT1 a multidomain, adaptor protein that regulates cellular processes, such as migration and protrusive activity, by bringing together various signaling molecules, including PIX, PAK, and paxillin. Phosphorylation on Ser-709 increases its binding to paxillin and regulates cellular protrusions. Possesses ADP-ribosylation factor (ARF) GTPase-activating protein (GAP) activity. Functions as part of a multi-molecular signaling complex that regulates membrane protrusions and cell migration. Regulates PLC-gamma activation by c-Src and integrates signal transduction by G protein coupled receptors (GPCR)s and tyrosine kinase receptors (TKRs). Note: This description may include information from UniProtKB.
Protein type: GAPs; Motility/polarity/chemotaxis; GAPs, ARF
Cellular Component: focal adhesion; membrane; cytoplasm
Molecular Function: protein binding; ARF GTPase activator activity; zinc ion binding
Biological Process: regulation of ARF GTPase activity; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity
Reference #:  Q9Y2X7 (UniProtKB)
Alt. Names/Synonyms: ARF GAP GIT1; ARF GTPase-activating protein GIT1; CAT-1; CAT1; Cool-associated and tyrosine-phosphorylated protein 1; G protein-coupled receptor kinase interacting ArfGAP 1; G protein-coupled receptor kinase interactor 1; G protein-coupled receptor kinase-interactor 1; GIT1; GRK-interacting protein 1
Gene Symbols: GIT1
Molecular weight: 84,341 Da
Basal Isoelectric point: 6.33  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GIT1

Protein Structure Not Found.


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Sites Implicated In
cell adhesion, induced: S46‑p
cell motility, altered: Y383‑p
cell motility, induced: S46‑p
cytoskeletal reorganization: Y383‑p, S700‑p
intracellular localization: S46‑p, Y383‑p
molecular association, regulation: S700‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 1 S46-p RSLGRHIsIVKHLRH
1 0 S54-p IVKHLRHsAWPPTLL
0 2 Y111-p SEFIRAKyQMLAFVH
0 6 T168-p FFHPEKGttPLHVAA
0 6 T169-p FHPEKGttPLHVAAK
0 1 T202-p SPDVNGRtPIDYARQ
0 12 Y224-p ERLVECQyELTDRLA
1 1 Y246-p PDHKNGHyIIPQMAD
0 1 - gap
0 1 - gap
0 1 S272 KKKLQALSNRLFEEL
4 1 Y284-p EELAMDVyDEVDRRE
2 0 Y321-p FLPVNPEySATRNQG
0 2 S359 KRRQQGKSLssPtDN
0 12 S361-p RQQGKSLssPtDNLE
0 32 S362-p QQGKSLssPtDNLEL
0 6 T364-p GKSLssPtDNLELsL
0 7 S370-p PtDNLELsLRsQsDL
0 12 S373-p NLELsLRsQsDLDDQ
0 30 S375-p ELsLRsQsDLDDQHD
5 188 Y383-p DLDDQHDyDsVAsDE
0 155 S385-p DDQHDyDsVAsDEDt
0 257 S388-p HDyDsVAsDEDtDQE
0 55 T392-p sVAsDEDtDQEPLRS
1 24 S410-p TRSNRARsMDssDLs
1 2 S413-p NRARsMDssDLsdGA
1 2 S414-p RARsMDssDLsdGAV
0 2 S417-p sMDssDLsdGAVTLQ
0 1 D418-ca MDssDLsdGAVTLQE
1 2 S448-p QQLMKVNssLSDELR
1 1 S449-p QLMKVNssLSDELRR
0 8 T480-p QPPGPVPtPPLPSER
0 1 T491-p PSERAEHtPMAPGGs
1 2 S498-p tPMAPGGstHRRDRQ
1 1 T499-p PMAPGGstHRRDRQA
1 28 S508-p RRDRQAFsMyEPGsA
1 162 Y510-p DRQAFsMyEPGsALK
0 8 S514-p FsMyEPGsALKPFGG
0 1 K517 yEPGsALKPFGGPPG
0 17 S536-p TRLQPFHstELEDDA
1 14 T537-p RLQPFHstELEDDAI
3 741 Y545-p ELEDDAIysVHVPAG
0 60 S546-p LEDDAIysVHVPAGL
0 231 Y554-p VHVPAGLyRIRKGVs
0 5 S561-p yRIRKGVsASAVPFt
0 1 S561 yRIRKGVSASAVPFt
0 2 S563 IRKGVsASAVPFtPs
0 1 A564 RKGVsASAVPFtPss
0 2 T568-p sASAVPFtPssPLLs
0 1 S570-p SAVPFtPssPLLsCs
0 16 S571-p AVPFtPssPLLsCsQ
0 1 S575-p tPssPLLsCsQEGSR
0 2 S577-p ssPLLsCsQEGSRHT
0 1 S585-p QEGSRHTsKLSRHGs
0 175 S592-p sKLSRHGsGADsDyE
0 153 S596-p RHGsGADsDyENtQs
1 197 Y598-p GsGADsDyENtQsGD
0 16 T601-p ADsDyENtQsGDPLL
0 1 S603-p sDyENtQsGDPLLGL
0 3 S630-p DFHPELEsLDGDLDP
0 1 K677-ac SFVPCSEkIHLAVTE
1 1 S700-p PALEPVRsSLRLLNA
0 1 S701 ALEPVRsSLRLLNAS
0 2 T753-p KAAKQLVtITTREKK
  GIT1 iso3  
S50 RSLGRHISIVKHLRH
S58 IVKHLRHSAWPPTLL
Y115 SEFIRAKYQMLAFVH
T172 FFHPEKGTTPLHVAA
T173 FHPEKGTTPLHVAAK
T206 SPDVNGRTPIDYARQ
Y228 ERLVECQYELTDRLA
Y250-p PDHKNGHyIIPQMAD
S265-p RSRQKCMsQsLDLSE
S267-p RQKCMsQsLDLSELA
S285 KKKLQALSNRLFEEL
Y297-p EELAMDVyDEVDRRE
Y334 FLPVNPEYSATRNQG
S372 KRRQQGKSLSsPTDN
S374 RQQGKSLSsPTDNLE
S375-p QQGKSLSsPTDNLEL
T377 GKSLSsPTDNLELSL
S383 PTDNLELSLRsQsDL
S386-p NLELSLRsQsDLDDQ
S388-p ELSLRsQsDLDDQHD
Y396-p DLDDQHDyDsVAsDE
S398-p DDQHDyDsVAsDEDt
S401-p HDyDsVAsDEDtDQE
T405-p sVAsDEDtDQEPLRS
S423-p TRSNRARsMDssDLS
S426-p NRARsMDssDLSDGA
S427-p RARsMDssDLSDGAV
S430 sMDssDLSDGAVTLQ
D431 MDssDLSDGAVTLQE
S461-p QQLMKVNssLSDELR
S462-p QLMKVNssLSDELRR
T493-p QPPGPVPtPPLPSER
T504 PSERAEHTPMAPGGs
S511-p TPMAPGGstHRRDRQ
T512-p PMAPGGstHRRDRQA
S521 RRDRQAFSMyEPGSA
Y523-p DRQAFSMyEPGSALK
S527 FSMyEPGSALKPFGG
K530 yEPGSALKPFGGPPG
S549-p TRLQPFHstELEDDA
T550-p RLQPFHstELEDDAI
Y558-p ELEDDAIysVHVPAG
S559-p LEDDAIysVHVPAGL
Y567-p VHVPAGLyRIRKGVS
S574 yRIRKGVSAsAVPFT
S574 yRIRKGVSAsAVPFT
S576-p IRKGVSAsAVPFTPS
A577 RKGVSAsAVPFTPSS
T581 SAsAVPFTPSSPLLS
S583 sAVPFTPSSPLLSCS
S584 AVPFTPSSPLLSCSQ
S588 TPSSPLLSCSQEGSR
S590 SSPLLSCSQEGSRHT
S598 QEGSRHTSKLSRHGs
S605-p SKLSRHGsGADsDyE
S609-p RHGsGADsDyENtQS
Y611-p GsGADsDyENtQSGD
T614-p ADsDyENtQSGDPLL
S616 sDyENtQSGDPLLGL
S643-p DFHPELEsLDGDLDP
K690 SFVPCSEKIHLAVTE
S713-p PALEPVRssLRLLNA
S714-p ALEPVRssLRLLNAS
T766 KAAKQLVTITTREKK
  mouse

 
S46 RSLGRHISIVKHLRH
S54 IVKHLRHSAWPPTLL
Y111 SEFIRAKYQMLAFVH
T168 FFHPEKGTTPLHVAA
T169 FHPEKGTTPLHVAAK
T202 SPDVNGRTPIDYARQ
Y224-p ERLVECQyELTDRLA
Y246 PDHKNGHYIIPQMAD
S261 RSRQKCMSQSLDLSE
S263 RQKCMSQSLDLSELA
S281-p KKKLQALsNRLFEEL
Y293-p EELAMDVyDEVDRRE
Y330 FLPVNPEYSATRNQG
S368-p KRRQQGKsLssPtDN
S370-p RQQGKsLssPtDNLE
S371-p QQGKsLssPtDNLEL
T373-p GKsLssPtDNLELsA
S379-p PtDNLELsARsQsEL
S382-p NLELsARsQsELDDQ
S384-p ELsARsQsELDDQHD
Y392-p ELDDQHDyDsVAsDE
S394-p DDQHDyDsVAsDEDt
S397-p HDyDsVAsDEDtDQE
T401-p sVAsDEDtDQEPLPS
S419-p TRNNRARsMDssDLs
S422-p NRARsMDssDLsDGA
S423-p RARsMDssDLsDGAV
S426-p sMDssDLsDGAVTLQ
D427 MDssDLsDGAVTLQE
S457-p QQLMKVNsSLSDELR
S460 MKVNsSLSDELRRLQ
P489 QPPGPVPPPSLPSER
T500 PSERAEHTLMGPGGS
S507 TLMGPGGSTHRRDRQ
T508 LMGPGGSTHRRDRQA
S517-p RRDRQAFsMyEPGsA
Y519-p DRQAFsMyEPGsALK
S523-p FsMyEPGsALKPFGG
K526 yEPGsALKPFGGTPG
S545-p TRLQPFHstELEDDA
T546-p RLQPFHstELEDDAI
Y554-p ELEDDAIysVHVPAG
S555-p LEDDAIysVHVPAGL
Y563-p VHVPAGLyRIRKGVs
S570-p yRIRKGVsAssVPFT
S570-gl yRIRKGVsAssVPFT
S572-p IRKGVsAssVPFTPS
S573-p RKGVsAssVPFTPSs
T577 sAssVPFTPSsPLLS
S579 ssVPFTPSsPLLSCS
S580-p sVPFTPSsPLLSCSQ
S584 TPSsPLLSCSQEGSR
S586 SsPLLSCSQEGSRHA
S594 QEGSRHASKLSRHGs
S601-p SKLSRHGsGADsDyE
S605-p RHGsGADsDyENtQS
Y607-p GsGADsDyENtQSGD
T610-p ADsDyENtQSGDPLL
S612 sDyENtQSGDPLLGL
S639-p ELHPELEsLDGDLDP
K686 SFVPCSEKIHLAVTE
S709 PALEPVRSSLRLLNA
S710 ALEPVRSSLRLLNAS
T762 KAAKQLVTITTREKK
  rat

 
S46 RSLGRHISIVKHLRH
S54 IVKHLRHSAWPPTLL
Y111 SEFIRAKYQMLAFVH
T168 FFHPEKGTTPLHVAA
T169 FHPEKGTTPLHVAAK
T202 SPDVNGRTPIDYARQ
Y224-p ERLVECQyELTDRLA
Y246 PDHKNGHYIIPQMAD
S261 RSRQKCMSQSLDLSE
S263 RQKCMSQSLDLSELA
S281 KKKLQALSNRLFEEL
Y293-p EELAMDVyDEVDRRE
Y330 FLPVNPEYSATRNQG
S368 KRRQQGKSLSSPTDN
S370 RQQGKSLSSPTDNLE
S371 QQGKSLSSPTDNLEL
T373 GKSLSSPTDNLELSA
S379 PTDNLELSARNQsDL
N382 NLELSARNQsDLDDQ
S384-p ELSARNQsDLDDQHD
Y392-p DLDDQHDyDsVAsDE
S394-p DDQHDyDsVAsDEDt
S397-p HDyDsVAsDEDtDQE
T401-p sVAsDEDtDQEPLPS
S419 TRNNRARSMDSSDLS
S422 NRARSMDSSDLSDGA
S423 RARSMDSSDLSDGAV
S426 SMDSSDLSDGAVTLQ
D427 MDSSDLSDGAVTLQE
S457 QQLMKVNSSLSDELR
S458 QLMKVNSSLSDELRK
V489 QPPGPVPVPSLPSER
T500 PSERAEHTLMGPGGS
S507 TLMGPGGSTHRRDRQ
T508 LMGPGGSTHRRDRQA
S517 RRDRQAFSMyEPGSA
Y519-p DRQAFSMyEPGSALk
S523 FSMyEPGSALkPFGG
K526-ub yEPGSALkPFGGAPG
S545 TRLQPFHSTELEDDA
T546 RLQPFHSTELEDDAI
Y554-p ELEDDAIySVHVPAG
S555 LEDDAIySVHVPAGL
Y563 VHVPAGLYRIRKGVS
S570 YRIRKGVSASSVTFT
S570 YRIRKGVSASSVTFT
S572 IRKGVSASSVTFTPS
S573 RKGVSASSVTFTPSS
T577 SASSVTFTPSSPLLS
S579 SSVTFTPSSPLLSSS
S580 SVTFTPSSPLLSSSQ
S584 TPSSPLLSSSQEGSR
S586 SSPLLSSSQEGSRHA
S594 QEGSRHASKLSRHGs
S601-p SKLSRHGsGAESDyE
S605 RHGsGAESDyENtQs
Y607-p GsGAESDyENtQsGE
T610-p AESDyENtQsGEPLL
S612-p SDyENtQsGEPLLGL
S639 ELHAELESLDGDPDP
K686 SFVPCSEKIHLAVTE
S709 PALEPVRSSLRLLNA
S710 ALEPVRSSLRLLNAS
T762 KAAKQLVTITTREKK
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