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Protein Page:
YB-1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
YB-1 a nuclear protein that binds to splice sites in pre-mRNA and regulates splice site selection. Binds and stabilizes cytoplasmic mRNA. Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors CCAAT-containing Y-box of HLA class II genes. Component of cytoplasmic messenger ribonucleoprotein particles (mRNPs). Interacts with AKT1, SFRS9, THOC4, MSH2, XRCC5, WRN and NCL. Can bind to DNA as a homomeric form, (EFI-A)n or as a heteromeric form in association with EFI-B. Homodimer in the presence of ATP. Involved in cisplatin resistance. Seems to be a negative regulatory factor. May play a role in both transcriptional and translational regulation of spermatogenesis. Found at very low levels at day 10 and levels increase at day 15 and persist throughout adulthood. Note: This description may include information from UniProtKB.
Protein type: RNA processing; RNA splicing; Transcription factor
Cellular Component: nucleoplasm; nuclear membrane; intracellular membrane-bound organelle; stress granule; cytoplasm; nucleus; ribonucleoprotein complex; U12-dependent spliceosome
Molecular Function: protein binding; DNA binding; RNA binding; double-stranded DNA binding; transcription factor activity; single-stranded DNA binding
Biological Process: nuclear mRNA splicing, via spliceosome; transcription from RNA polymerase II promoter; negative regulation of striated muscle cell differentiation; regulation of transcription, DNA-dependent; in utero embryonic development; positive regulation of cell division; RNA splicing; gene expression; negative regulation of transcription from RNA polymerase II promoter
Reference #:  P67809 (UniProtKB)
Alt. Names/Synonyms: BP-8; CBF-A; CCAAT-binding transcription factor I subunit A; CSDA2; CSDB; DBPB; DNA-binding protein B; EFI-A; Enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP-1; NSEP1; nuclease sensitive element binding protein 1; Nuclease-sensitive element-binding protein 1; Y box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YB-1; YB1; YBOX1; YBX1
Gene Symbols: YBX1
Molecular weight: 35,924 Da
Basal Isoelectric point: 9.87  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

YB-1

Protein Structure Not Found.


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Sites Implicated In
carcinogenesis, altered: S102‑p
cell growth, altered: S102‑p
transcription, altered: S102‑p
activity, induced: S102‑p
intracellular localization: S102‑p, Y281‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 3 S2-p ______MssEAEtQQ
0 2 S3-p _____MssEAEtQQP
0 1 T7-p _MssEAEtQQPPAAP
0 1 S21-p PPAAPALsAADTKPG
0 3 K26 ALsAADTKPGTTGsG
0 1 S32-p TKPGTTGsGAGsGGP
0 1 S36-p TTGsGAGsGGPGGLT
0 1 T43 sGGPGGLTsAAPAGG
0 2 S44-p GGPGGLTsAAPAGGD
0 4 K58-ub DKKVIATkVLGTVkW
0 6 K64-ac TkVLGTVkWFNVRNG
0 12 K64-ub TkVLGTVkWFNVRNG
1 4 T80-p GFINRNDtkEDVFVH
0 1 K81-ac FINRNDtkEDVFVHQ
0 16 K81-ub FINRNDtkEDVFVHQ
0 1 T89-p EDVFVHQtAIkkNNP
0 112 K92-ub FVHQtAIkkNNPRKY
0 42 K93-ub VHQtAIkkNNPRKYL
5 18 S102-p NPRKYLRsVGDGEtV
0 1 T108-p RsVGDGEtVEFdVVE
0 1 D112-ca DGEtVEFdVVEGEkG
0 2 K118-ub FdVVEGEkGAEAANV
0 1 S136-p GGVPVQGskYAADRN
0 2 K137-ac GVPVQGskYAADRNH
0 87 K137-ub GVPVQGskYAADRNH
0 42 Y145-p YAADRNHyRRYPRRR
0 154 Y158-p RRGPPRNyQQNyQNs
0 336 Y162-p PRNyQQNyQNsEsGE
0 70 S165-p yQQNyQNsEsGEkNE
0 46 S167-p QNyQNsEsGEkNEGs
0 55 K170-ub QNsEsGEkNEGsEsA
0 65 S174-p sGEkNEGsEsAPEGQ
0 63 S176-p EkNEGsEsAPEGQAQ
0 1 R185 PEGQAQQRRPyRRRR
1 0 Y188-p QAQQRRPyRRRRFPP
0 1 R189 AQQRRPyRRRRFPPy
0 15 Y196-p RRRRFPPyyMrrPYG
0 9 Y197-p RRRFPPyyMrrPYGR
0 10 R199-m1 RFPPyyMrrPYGRRP
0 1 R199-m2 RFPPyyMrrPYGRRP
0 1 R200-m1 FPPyyMrrPYGRRPQ
0 166 Y208-p PYGRRPQysNPPVQG
0 22 S209-p YGRRPQysNPPVQGE
0 5 Y238-p RPVRQNMyrGYRPRF
0 1 R239-m1 PVRQNMyrGYRPRFR
0 36 K264-ub EDGNEEDkENQGDEt
0 20 T271-p kENQGDEtQGQQPPQ
0 2 R279 QGQQPPQRRyRRNFN
1 0 Y281-p QQPPQRRyRRNFNyR
0 14 Y287-p RyRRNFNyRRRRPEN
0 4 K296-ub RRRPENPkPQDGkET
0 42 K301-ub NPkPQDGkETkAADP
0 65 K304-ub PQDGkETkAADPPAE
0 9 S313-p ADPPAENssAPEAEQ
0 33 S314-p DPPAENssAPEAEQG
2900 : Phospho-YB1 (Ser102) (C34A2) Rabbit mAb
  mouse

 
S2 ______MSSEAETQQ
S3 _____MSSEAETQQP
T7 _MSSEAETQQPPAAP
S19 AAPAAALSAADTkPG
K24-ub ALSAADTkPGSTGSG
S30 TkPGSTGSGAGSGGP
S34 STGSGAGSGGPGGLT
T41 SGGPGGLTSAAPAGG
S42 GGPGGLTSAAPAGGD
K56-ub DKKVIATkVLGTVkW
K62 TkVLGTVKWFNVRNG
K62-ub TkVLGTVkWFNVRNG
T78-p GFINRNDtkEDVFVH
K79 FINRNDtKEDVFVHQ
K79-ub FINRNDtkEDVFVHQ
T87 EDVFVHQTAIkkNNP
K90-ub FVHQTAIkkNNPRKY
K91-ub VHQTAIkkNNPRKYL
S100-p NPRKYLRsVGDGETV
T106 RsVGDGETVEFDVVE
D110 DGETVEFDVVEGEKG
K116 FDVVEGEKGAEAANV
S134 GGVPVQGSkYAADRN
K135-ac GVPVQGSkYAADRNH
K135-ub GVPVQGSkYAADRNH
Y143-p YAADRNHyRRYPRRR
Y156-p RRGPPRNyQQNyQNs
Y160-p PRNyQQNyQNsEsGE
S163-p yQQNyQNsEsGEkNE
S165-p QNyQNsEsGEkNEGs
K168-ub QNsEsGEkNEGsEsA
S172-p sGEkNEGsEsAPEGQ
S174-p EkNEGsEsAPEGQAQ
R183 PEGQAQQRRPYrRRR
Y186 QAQQRRPYrRRRFPP
R187-m1 AQQRRPYrRRRFPPY
Y194 rRRRFPPYYMrRPYA
Y195 RRRFPPYYMrRPYAR
R197-m1 RFPPYYMrRPYARRP
R197 RFPPYYMRRPYARRP
R198 FPPYYMrRPYARRPQ
Y206-p PYARRPQysNPPVQG
S207-p YARRPQysNPPVQGE
Y236 RPVRQNMYrGYRPRF
R237-m1 PVRQNMYrGYRPRFR
K262-ub EDGNEEDkENQGDEt
T269-p kENQGDEtQGQQPPQ
R277 QGQQPPQRRYRRNFN
Y279 QQPPQRRYRRNFNYR
Y285 RYRRNFNYRRRRPEN
K294-ub RRRPENPkPQDGkET
K299-ub NPkPQDGkETkAADP
K302-ub PQDGkETkAADPPAE
S311-p ADPPAENssAPEAEQ
S312-p DPPAENssAPEAEQG
2900 : Phospho-YB1 (Ser102) (C34A2) Rabbit mAb
  rat

 
S2-p ______MsSEAETQQ
S3 _____MsSEAETQQP
T7 _MsSEAETQQPPAAP
S19 AAPAAALSAADTKPG
K24 ALSAADTKPGSTGSG
S30 TKPGSTGSGAGSGGP
S34 STGSGAGSGGPGGLt
T41-p SGGPGGLtsAAPAGG
S42-p GGPGGLtsAAPAGGD
K56 DKKVIATKVLGTVKW
K62 TKVLGTVKWFNVRNG
K62 TKVLGTVKWFNVRNG
T78 GFINRNDTkEDVFVH
K79-ac FINRNDTkEDVFVHQ
K79 FINRNDTKEDVFVHQ
T87 EDVFVHQTAIKKNNP
K90 FVHQTAIKKNNPRKY
K91 VHQTAIKKNNPRKYL
S100-p NPRKYLRsVGDGETV
T106 RsVGDGETVEFDVVE
D110 DGETVEFDVVEGEKG
K116 FDVVEGEKGAEAANV
S134 GGVPVQGSkYAADRN
K135-ac GVPVQGSkYAADRNH
K135 GVPVQGSKYAADRNH
Y143 YAADRNHYRRYPRRR
Y156 RRGPPRNYQQNYQNs
Y160 PRNYQQNYQNsEsGE
S163-p YQQNYQNsEsGEKNE
S165-p QNYQNsEsGEKNEGs
K168 QNsEsGEKNEGsEsA
S172-p sGEKNEGsEsAPEGQ
S174-p EKNEGsEsAPEGQAQ
R183-m1 PEGQAQQrRPYRRRR
Y186 QAQQrRPYRRRRFPP
R187 AQQrRPYRRRRFPPY
Y194 RRRRFPPYYMRRPYA
Y195 RRRFPPYYMRRPYAR
R197 RFPPYYMRRPYARRP
R197 RFPPYYMRRPYARRP
R198 FPPYYMRRPYARRPQ
Y206 PYARRPQYsNPPVQG
S207-p YARRPQYsNPPVQGE
Y236 RPVRQNMYRGYRPRF
R237 PVRQNMYRGYRPRFR
K262 EDGNEEDKENQGDET
T269 KENQGDETQGQQPPQ
R277-m1 QGQQPPQrRYRRNFN
Y279 QQPPQrRYRRNFNYR
Y285 RYRRNFNYRRRRPEN
K294 RRRPENPKPQDGKET
K299 NPKPQDGKETKAADP
K302 PQDGKETKAADPPAE
S311 ADPPAENSsAPEAEQ
S312-p DPPAENSsAPEAEQG
2900 : Phospho-YB1 (Ser102) (C34A2) Rabbit mAb
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