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Protein Page:
GRF-1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GRF-1 the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: GAPs, Rac/Rho; Transcription factor; GAPs; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19q13.3
Cellular Component: cytosol; nucleus; actin cytoskeleton
Molecular Function: GTP binding; DNA binding; Rho GTPase activator activity; transcription corepressor activity
Biological Process: regulation of cell shape; axon guidance; regulation of small GTPase mediated signal transduction; camera-type eye development; transcription, DNA-dependent; small GTPase mediated signal transduction; forebrain development; neural tube closure; negative regulation of vascular permeability; negative regulation of transcription, DNA-dependent; positive regulation of Rho GTPase activity
Reference #:  Q9NRY4 (UniProtKB)
Alt. Names/Synonyms: ARHGAP35; glucocorticoid receptor DNA binding factor 1; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; GRF1; GRLF1; KIAA1722; MGC10745; p190-A; P190A; p190ARhoGAP; p190RhoGAP; Rho GAP p190A
Gene Symbols: ARHGAP35
Molecular weight: 170,514 Da
Basal Isoelectric point: 6.2  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

GRF-1

Protein Structure Not Found.


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Sites Implicated In
cytoskeletal reorganization: S1221‑p, T1226‑p
activity, induced: Y1105‑p
activity, inhibited: S1150‑p, T1173‑p, S1174‑p, T1226‑p, S1236‑p
molecular association, regulation: S1150‑p, T1173‑p, S1174‑p, S1221‑p, T1226‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 3 Y14 QDVRIPTYNISVVGL
0 1 S46-p CNRFVRPsADEFHLD
0 1 Y133-p ASAEKLMyFCtDQLG
0 1 T136-p EKLMyFCtDQLGLEQ
0 1 K193-ac NQLAKTKkPIVVVLT
0 1 Y209-p CDEGVERyIRDAHTF
0 1 S296 NENWLSVSRKMQASP
1 0 Y308 ASPEYQDYVYLEGTQ
0 1 K368 LSCIKAKKLLETKPE
0 1 S575-p AKIEHLIssRFIRPS
0 1 S576-p KIEHLIssRFIRPSD
0 21 S589-p SDRNQKNsLSDPNID
0 1 S667-p GCLCLYNsKESLSYV
0 1 T685 IEKSRESTLGRRDNH
0 2 S769-p KRNLNLVsstAsIKD
0 5 S770-p RNLNLVsstAsIKDL
0 3 T771-p NLNLVsstAsIKDLA
0 11 S773-p NLVsstAsIKDLADV
0 1 Y855-p KSRLVHGyIVFYSAK
0 1 T963-p NAAEACStTEEVFNs
0 20 S970-p tTEEVFNsPRAGsPL
0 27 S975-p FNsPRAGsPLCNsNL
0 3 S980-p AGsPLCNsNLQDsEE
0 14 S985-p CNsNLQDsEEDIEPS
0 4 S1001-p SLFREDTsLPsLsKD
0 1 S1004-p REDTsLPsLsKDHSK
0 1 S1006-p DTsLPsLsKDHSKLS
0 5 S1057-p EITKGDLsYLDQGHR
0 86 S1070-p HRDGQRKsVsSsPWL
0 38 S1072-p DGQRKsVsSsPWLPQ
0 5 S1074-p QRKsVsSsPWLPQDG
0 35 S1085-p PQDGFDPsDyAEPMD
1 1074 Y1087-p DGFDPsDyAEPMDAV
0 1 K1096-ub EPMDAVVkPRNEEEN
2 3242 Y1105-p RNEEENIysVPHDst
0 180 S1106-p NEEENIysVPHDstQ
0 3 S1111-p IysVPHDstQGKIIT
0 1 T1112-p ysVPHDstQGKIITI
0 1 S1127-p RNINKAQsNGsGNGs
0 3 S1130-p NKAQsNGsGNGsDsE
0 18 S1134-p sNGsGNGsDsEMDtS
0 1 S1136-p GsGNGsDsEMDtSsL
0 1 T1140-p GsDsEMDtSsLERGR
0 3 S1142-p DsEMDtSsLERGRKV
1 76 S1150-p LERGRKVsIVsKPVL
0 1 S1153-p GRKVsIVsKPVLyRT
0 1 Y1158-p IVsKPVLyRTRCTRL
1 2 T1173-p GRFASYRtsFsVGsD
1 1 S1174-p RFASYRtsFsVGsDD
0 4 S1176-p ASYRtsFsVGsDDEL
0 60 S1179-p RtsFsVGsDDELGPI
0 1 S1195-p KKEEDQAsQGyKGDN
0 2 Y1198-p EDQAsQGyKGDNAVI
0 2 Y1207-p GDNAVIPyETDEDPR
1 2 S1221-p RRRNILRsLRRNtKK
2 1 T1226-p LRsLRRNtKKPKPKP
2 0 S1236-p PKPKPRPsITKATWE
0 1 K1259 TTVVTPEKPIPIFIE
0 1 Y1271 FIERCIEYIEATGLS
0 1 Y1283 GLSTEGIYRVSGNks
0 1 K1289-ub IYRVSGNksEMESLQ
0 2 S1290-p YRVSGNksEMESLQR
  mouse

 
Y14 QDVRIPTYNISVVGL
S46 CNRFVRPSADEFHLD
Y133 ASAEKLMYFCTDQLG
T136 EKLMYFCTDQLGLEQ
K193 NQLAKTKKPIVVVLT
Y209 CDEGVERYIRDAHTF
S296-p NENWPSVsRKMQASP
Y308-p ASPEYQDyVYLEGTQ
K368-ub LSCIKAKkLLETKPE
S575 AKIEHLISSRFIRPS
S576 KIEHLISSRFIRPSD
S589-p SDRNQKNsLSDLNID
S667 GCLCLYNSKESLSYV
T685-p IEKSREStLGRRDNH
S769-p KRNLNLVsstAsIKD
S770-p RNLNLVsstAsIKDL
T771-p NLNLVsstAsIKDLA
S773-p NLVsstAsIKDLADV
Y855 KSRLVHGYIVFYSAK
T963 NVAEACSTTEEVFNs
S970-p TTEEVFNsPRAGsPL
S975-p FNsPRAGsPLCNsNL
S980-p AGsPLCNsNLQDsEE
S985-p CNsNLQDsEEDVEPP
T1002 HLFREDATLPSLSKD
S1005 REDATLPSLSKDHSK
S1007 DATLPSLSKDHSKFS
S1057-p FDITKDLsYLDQGHR
S1070-p HREGQRKsMsSSPWM
S1072-p EGQRKsMsSSPWMPQ
S1074 QRKsMsSSPWMPQDG
S1085-p PQDGFDPsDyAEPMD
Y1087-p DGFDPsDyAEPMDAV
K1096 EPMDAVVKPRNEEEN
Y1105-p RNEEENIysVPHDST
S1106-p NEEENIysVPHDSTQ
S1111 IysVPHDSTQGKIIT
T1112 ysVPHDSTQGKIITI
S1127 RNINKAQSNGSGNGs
S1130 NKAQSNGSGNGsDsE
S1134-p SNGSGNGsDsEMDTS
S1136-p GSGNGsDsEMDTSsL
T1140 GsDsEMDTSsLERGR
S1142-p DsEMDTSsLERGRKV
S1150-p LERGRKVsAVSKPVL
S1153 GRKVsAVSKPVLYRT
Y1158 AVSKPVLYRTRCTRL
T1173-p GRFASYRtSFsVGsD
S1174 RFASYRtSFsVGsDD
S1176-p ASYRtSFsVGsDDEL
S1179-p RtSFsVGsDDELGPI
S1195 KKEEDQASQGYKGDN
Y1198 EDQASQGYKGDNAVI
Y1207 GDNAVIPYETDEDPR
S1221 RRRNILRSLRRNTKK
T1226 LRSLRRNTKKPKPKP
S1236 PKPKPRPSITKATWE
K1259 TTVVTPEKPIPIFIE
Y1271 FIERCIEYIEATGLS
Y1283 GLSTEGIYRVSGNKs
K1289 IYRVSGNKsEMESLQ
S1290-p YRVSGNKsEMESLQR
  rat

 
Y14-p QDVRIPTyNISVVGL
S46 CNRFVRPSADEFHLD
Y133 ASAEKLMYFCTDQLG
T136 EKLMYFCTDQLGLEQ
K193 NQLAKTKKPIVIVLT
Y209 CDEGVERYIRDAHTF
S296 NENWLSVSRKMQASP
Y308 ASPEYQDYVYLEGTQ
K368 LSCIKAKKLLETKPE
S575 AKIEHLISSRFIRPS
S576 KIEHLISSRFIRPSD
S589-p SDRNQKNsLSDPNID
S667 GCLCLYNSKESLSYV
T685 IEKSRESTLGRRDNH
S769-p KRNLNLVssTAsIKD
S770-p RNLNLVssTAsIKDL
T771 NLNLVssTAsIKDLA
S773-p NLVssTAsIKDLADV
Y855 KSRLVHGYIVFYSAK
T963 NVAEACSTTEEVFNS
S970 TTEEVFNSPRAGsPL
S975-p FNSPRAGsPLCNsNL
S980-p AGsPLCNsNLQDsEE
S985-p CNsNLQDsEEDVEPP
T1002 HLFREDATLPSLSKD
S1005 REDATLPSLSKDHSK
S1007 DATLPSLSKDHSKFS
S1057 FEITKDLSYLDQGHR
S1070 HREGQRKSMSSSPWM
S1072 EGQRKSMSSSPWMPQ
S1074 QRKSMSSSPWMPQDG
S1085 PQDGFDPSDYAEPMD
Y1087 DGFDPSDYAEPMDAV
K1096 EPMDAVVKPRNEEEN
Y1105-p RNEEENIySVPHDST
S1106 NEEENIySVPHDSTQ
S1111 IySVPHDSTQGKIIT
T1112 ySVPHDSTQGKIITI
S1127 RNINKAQSNGSGNGs
S1130 NKAQSNGSGNGsDSE
S1134-p SNGSGNGsDSEMDTS
S1136 GSGNGsDSEMDTSSL
T1140 GsDSEMDTSSLERGR
S1142 DSEMDTSSLERGRKV
S1150 LERGRKVSAVSKPVL
S1153 GRKVSAVSKPVLYRT
- under review  
T1173 GRFASYRTSFSVGsD
S1174 RFASYRTSFSVGsDD
S1176 ASYRTSFSVGsDDEL
S1179-p RTSFSVGsDDELGPI
S1195 KKEEDQASQGYKGDN
Y1198 EDQASQGYKGDNAVI
Y1207 GDNAVIPYETDEDPR
S1221 RRRNILRSLRRNTKK
T1226 LRSLRRNTKKPKPKP
S1236 PKPKPRPSITKATWE
K1259-ub TTVVTPEkPIPIFIE
Y1271-p FIERCIEyIEATGLS
Y1283-p GLSTEGIyRVSGNKS
K1289 IyRVSGNKSEMESLQ
S1290 yRVSGNKSEMESLQR
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