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Protein Page:
UBE1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
UBE1 an enzyme of the ubiquitin-activating E1 family. Adenylates ubiquitin at its carboxy-terminal glycine residue and thereafter links this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester. There are two active sites within the E1 molecule, allowing it to accomodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. Complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. Note: This description may include information from UniProtKB.
Protein type: EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: Xp11.23
Cellular Component: heterochromatin; desmosome; mitochondrion; lysosomal membrane; cytoplasm; endosome membrane; rough endoplasmic reticulum membrane; cytosol; nucleus
Molecular Function: protein binding; ubiquitin-protein ligase activity; ubiquitin activating enzyme activity; ATP binding
Biological Process: modification-dependent protein catabolic process; cell death; protein ubiquitination; response to DNA damage stimulus
Reference #:  P22314 (UniProtKB)
Alt. Names/Synonyms: A1S9; A1S9T; A1S9T and BN75 temperature sensitivity complementing; A1ST; AMCX1; GXP1; MGC4781; POC20; POC20 centriolar protein homolog; Protein A1S9; SMAX2; UBA1; UBA1, ubiquitin-activating enzyme E1 homolog A; UBA1A; UBE1; UBE1X; Ubiquitin-activating enzyme E1; ubiquitin-like modifier activating enzyme 1; Ubiquitin-like modifier-activating enzyme 1
Gene Symbols: UBA1
Molecular weight: 117,849 Da
Basal Isoelectric point: 5.49  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

UBE1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
2 3 S4-p ____MSSsPLSKKRR
0 36 S13-p LSKKRRVsGPDPKPG
0 8 S21-p GPDPKPGsNCsPAQs
0 8 S24-p PKPGsNCsPAQsVLs
0 4 S28-p sNCsPAQsVLsEVPS
0 3 S31-p sPAQsVLsEVPSVPT
0 36 S46-p NGMAKNGsEADIDEG
0 386 Y55-p ADIDEGLysRQLyVL
0 2 S56-p DIDEGLysRQLyVLG
0 11 Y60-p GLysRQLyVLGHEAM
0 2 K68-ac VLGHEAMkRLQTSsV
0 3 K68-ub VLGHEAMkRLQTSsV
0 1 K68-sc VLGHEAMkRLQTSsV
0 1 S74-p MkRLQTSsVLVSGLR
0 9 K89-ac GLGVEIAkNIILGGV
0 2 K89-ub GLGVEIAkNIILGGV
0 1 S131 GKNRAEVSQPRLAEL
0 1 S140-p PRLAELNsYVPVTAY
0 19 K185-ub FCHNRGIkLVVADtR
0 1 T191-p IkLVVADtRGLFGQL
0 2 Y273-p EIKVLGPytFSICDT
0 2 T274-p IKVLGPytFSICDTS
0 1 S281 tFSICDTSNFsDyIR
0 1 S284-p ICDTSNFsDyIRGGI
0 1 Y286-p DTSNFsDyIRGGIVs
0 1 S293-p yIRGGIVsQVkVPKK
0 2 K296-ub GGIVsQVkVPKKISF
0 1 K299 VsQVkVPKKISFksL
0 3 K304-ub VPKKISFksLVAsLA
0 3 S305-p PKKISFksLVAsLAE
0 2 S309-p SFksLVAsLAEPDFV
0 1 T318-p AEPDFVVtDFAkFSR
0 2 K322-ub FVVtDFAkFSRPAQL
0 1 Q374 ARALPAVQQNNLDED
0 1 N377 LPAVQQNNLDEDLIR
0 3 K385-ub LDEDLIRkLAyVAAG
0 17 Y388-p DLIRkLAyVAAGDLA
0 2 K411-ub LAAQEVMkACSGkFM
0 1 K416-ub VMkACSGkFMPIMQW
0 2 K443-ub KEVLTEDkCLQRQNR
0 1 S460-p GQVAVFGsDLQEkLG
0 1 K465-ac FGsDLQEkLGkQkYF
0 10 K465-ub FGsDLQEkLGkQkYF
0 3 K468-ub DLQEkLGkQkYFLVG
0 1 K470-ac QEkLGkQkYFLVGAG
0 16 K470-ub QEkLGkQkYFLVGAG
0 2 K526-ub FRPWDVTkLkSDTAA
0 9 K528-ub PWDVTkLkSDTAAAA
0 1 K528-sc PWDVTkLkSDTAAAA
0 7 Y560-p GPDTERIyDDDFFQN
0 3 Y590-p YMDRRCVyYRkPLLE
0 1 K593-ac RRCVyYRkPLLESGT
0 71 K593-ub RRCVyYRkPLLESGT
0 1 T603-p LESGTLGtkGNVQVV
0 1 K604-ac ESGTLGtkGNVQVVI
0 33 K604-ub ESGTLGtkGNVQVVI
0 1 T615-p QVVIPFLtESYSSSQ
0 1 K627-ac SSQDPPEksIPICTL
0 35 K627-ub SSQDPPEksIPICTL
0 1 S628-p SQDPPEksIPICTLk
0 114 K635-ub sIPICTLkNFPNAIE
0 30 K657-ub DEFEGLFkQPAENVN
0 1 P670 VNQYLTDPkFVERTL
0 3 K671-ac NQYLTDPkFVERTLR
0 17 K671-ub NQYLTDPkFVERTLR
0 1 K671-sc NQYLTDPkFVERTLR
0 1 T682-p RTLRLAGtQPLEVLE
0 73 K746-ub APFWSGPkRCPHPLT
0 1 S781-p QTYGLTGsQDRAAVA
0 1 T789-p QDRAAVAtFLQsVQV
0 1 S793-p AVAtFLQsVQVPEFt
0 6 T800-p sVQVPEFtPksGVkI
0 8 K802-ub QVPEFtPksGVkIHV
0 1 S803-p VPEFtPksGVkIHVs
0 11 K806-ub FtPksGVkIHVsDQE
0 17 S810-p sGVkIHVsDQELQsA
0 12 S816-p VsDQELQsANAsVDD
0 12 S820-p ELQsANAsVDDsRLE
0 1 S824-p ANAsVDDsRLEELKA
0 1 T832-p RLEELKAtLPsPDkL
0 24 S835-p ELKAtLPsPDkLPGF
0 3 K838-ub AtLPsPDkLPGFkMY
0 2 K843-ub PDkLPGFkMYPIDFE
0 1 S866-p MDFIVAAsNLRAENy
0 15 Y873-p sNLRAENyDIPSADR
0 2 P876 RAENyDIPSADRHKS
0 1 K882 IPSADRHKSKLIAGk
0 1 S883 PSADRHKSKLIAGkI
0 28 K889-ub KSKLIAGkIIPAIAT
0 4 K923-ub HRQLDSYkNGFLNLA
0 1 T971 QPNGEEMTLKQFLDY
0 1 K980-ac KQFLDYFkTEHKLEI
0 1 K980-ub KQFLDYFkTEHKLEI
0 4 K1024-ac EIVSRVSkRKLGRHV
  mouse

 
S4 ____MSSSPLSKKRR
S13-p LSKKRRVsGPDPKPG
S21-p GPDPKPGsNCsPAQs
S24-p PKPGsNCsPAQsALs
S28-p sNCsPAQsALsEVSS
S31-p sPAQsALsEVSSVPT
S46-p NGMAKNGsEADIDES
Y55-p ADIDESLySRQLYVL
S56 DIDESLySRQLYVLG
Y60 SLySRQLYVLGHEAM
K68 VLGHEAMKMLQTSSV
K68 VLGHEAMKMLQTSSV
K68 VLGHEAMKMLQTSSV
S74 MKMLQTSSVLVSGLR
K89 GLGVEIAKNIILGGV
K89 GLGVEIAKNIILGGV
S131-p GKNRAEVsQPRLAEL
S140 PRLAELNSYVPVTAY
K185-ub FCHSRGIkLVVADTR
T191 IkLVVADTRGLFGQL
Y273 EIKVLGPYTFSICDT
T274 IKVLGPYTFSICDTs
S281-p TFSICDTsNFSDYIR
S284 ICDTsNFSDYIRGGI
Y286 DTsNFSDYIRGGIVS
S293 YIRGGIVSQVkVPkK
K296-ub GGIVSQVkVPkKISF
K299-ub VSQVkVPkKISFkSL
K304-ub VPkKISFkSLPASLV
S305 PkKISFkSLPASLVE
S309 SFkSLPASLVEPDFV
T318 VEPDFVMTDFAkYSR
K322-ub FVMTDFAkYSRPAQL
K374-ub ARSPPSVkQNsLDED
S377-p PPSVkQNsLDEDLIR
K385-ub LDEDLIRkLAyVAAG
Y388-p DLIRkLAyVAAGDLA
K411 LAAQEVMKACSGKFM
K416 VMKACSGKFMPIMQW
K443-ub KEALTEEkCLPRQNR
S460 GQVAVFGSDFQEkLS
K465-ac FGSDFQEkLSKQKYF
K465-ub FGSDFQEkLSKQKYF
K468 DFQEkLSKQKYFLVG
K470 QEkLSKQKYFLVGAG
K470 QEkLSKQKYFLVGAG
K526-ub FRPWDVTkLkSDTAA
K528-ub PWDVTkLkSDTAAAA
K528 PWDVTkLKSDTAAAA
Y560 GPDTERIYDDDFFQN
Y590 YMDRRCVYYRkPLLE
K593 RRCVYYRKPLLESGT
K593-ub RRCVYYRkPLLESGT
T603 LESGTLGTkGNVQVV
K604 ESGTLGTKGNVQVVI
K604-ub ESGTLGTkGNVQVVI
T615 QVVIPFLTESYSSSQ
K627 SSQDPPEKSIPICTL
K627-ub SSQDPPEkSIPICTL
S628 SQDPPEkSIPICTLk
K635-ub SIPICTLkNFPNAIE
K657-ub DEFEGLFkQPAENVN
S670-p VNQYLTDskFVERTL
K671-ac NQYLTDskFVERTLR
K671-ub NQYLTDskFVERTLR
K671 NQYLTDsKFVERTLR
T682 RTLRLAGTQPLEVLE
K746-ub APFWSGPkRCPHPLT
S781 QTYGLTGSQDRAAVA
S789 QDRAAVASLLQSVQV
S793 AVASLLQSVQVPEFt
T800-p SVQVPEFtPkSGVkI
K802-ub QVPEFtPkSGVkIHV
S803 VPEFtPkSGVkIHVs
K806-ub FtPkSGVkIHVsDQE
S810-p SGVkIHVsDQELQsA
S816-p VsDQELQsANAsVDD
S820-p ELQsANAsVDDSRLE
S824 ANAsVDDSRLEELKA
T832-p RLEELKAtLPsPDkL
S835-p ELKAtLPsPDkLPGF
K838-ub AtLPsPDkLPGFkMY
K843-ub PDkLPGFkMYPIDFE
S866 MDFIVAASNLRAENY
Y873 SNLRAENYDIsPADR
S876-p RAENYDIsPADRHks
K882-ub IsPADRHksKLIAGk
S883-p sPADRHksKLIAGkI
K889-ub ksKLIAGkIIPAIAT
K923-ub HQQLDSYkNGFLNLA
T971-p QPNGEEMtLKQFLDY
K980 KQFLDYFKTEHKLEI
K980 KQFLDYFKTEHKLEI
K1024 EIVSRVSKRKLGRHV
  rat

 
S4-p ____MSSsPLSKKRR
S13-p LSKKRRVsGPDPKPG
S21 GPDPKPGSNCSSAQS
S24 PKPGSNCSSAQSVLS
S28 SNCSSAQSVLSEVSS
S31 SSAQSVLSEVSSVPT
S46-p NGMAKNGsEADIDES
Y55 ADIDESLYSRQLYVL
S56 DIDESLYSRQLYVLG
Y60 SLYSRQLYVLGHEAM
K68 VLGHEAMKMLQTSSV
K68 VLGHEAMKMLQTSSV
K68 VLGHEAMKMLQTSSV
S74 MKMLQTSSVLVSGLR
K89 GLGVEIAKNIILGGV
K89 GLGVEIAKNIILGGV
S131 GKNRAEVSQPRLAEL
S140 PRLAELNSYVPVTAY
K185 FCHSRGIKLVVADTR
T191 IKLVVADTRGLFGQL
Y273 EIKVLGPYTFSICDT
T274 IKVLGPYTFSICDTS
S281 TFSICDTSNFSDYIR
S284 ICDTSNFSDYIRGGI
Y286 DTSNFSDYIRGGIVS
S293 YIRGGIVSQVKVPKK
K296 GGIVSQVKVPKKISF
K299 VSQVKVPKKISFKSL
K304 VPKKISFKSLPASLA
S305 PKKISFKSLPASLAE
S309 SFKSLPASLAEPDFV
T318 AEPDFVMTDFAKYSR
K322 FVMTDFAKYSRPAQL
Q374 ARSPPAVQQDNVDED
N377 PPAVQQDNVDEDLIR
K385 VDEDLIRKLAyVAAG
Y388-p DLIRKLAyVAAGDLA
K411 LAAQEVMKACSGKFM
K416 VMKACSGKFMPIMQW
K443 KEALTEDKCLPRQNR
S460 GQVAVFGSDLQEkLG
K465-ac FGSDLQEkLGKQKYF
K465 FGSDLQEKLGKQKYF
K468 DLQEkLGKQKYFLVG
K470 QEkLGKQKYFLVGAG
K470 QEkLGKQKYFLVGAG
K526 FRPWDVTKLKSDTAA
K528 PWDVTKLKSDTAAAA
K528 PWDVTKLKSDTAAAA
Y560 GPDTERIYDDDFFQN
Y590 YMDRRCVYYRKPLLE
K593 RRCVYYRKPLLESGT
K593 RRCVYYRKPLLESGT
T603 LESGTLGTKGNVQVV
K604 ESGTLGTKGNVQVVI
K604 ESGTLGTKGNVQVVI
T615 QVVIPFLTESYSSSQ
K627 SSQDPPEKSIPICTL
K627 SSQDPPEKSIPICTL
S628 SQDPPEKSIPICTLK
K635 SIPICTLKNFPNAIE
K657 DEFEGLFKQPAENVN
S670 VNQYLTDSkFVERTL
K671-ac NQYLTDSkFVERTLR
K671 NQYLTDSKFVERTLR
K671 NQYLTDSKFVERTLR
T682 RTLRLAGTQPLEVLE
K746 APFWSGPKRCPHPLT
S781 QTYGLTGSQDRAAVA
S789 QDRAAVASLLQSVQV
S793 AVASLLQSVQVPEFT
T800 SVQVPEFTPKSGVKI
K802 QVPEFTPKSGVKIHV
S803 VPEFTPKSGVKIHVS
K806 FTPKSGVKIHVSDQE
S810 SGVKIHVSDQELQsA
S816-p VSDQELQsANAsVDD
S820-p ELQsANAsVDDSRLE
S824 ANAsVDDSRLEELKA
T832 RLEELKATLPsPDKL
S835-p ELKATLPsPDKLPGF
K838 ATLPsPDKLPGFKMY
K843 PDKLPGFKMYPIDFE
S866 MDFIVAASNLRAENy
Y873-p SNLRAENyDISPADR
S876 RAENyDISPADRHKS
K882 ISPADRHKSKLIAGK
S883 SPADRHKSKLIAGKI
K889 KSKLIAGKIIPAIAT
K923 HQQLDSYKNGFLNLA
T971 QPNGEEMTLKQFLDY
K980 KQFLDYFKTEHKLEI
K980 KQFLDYFKTEHKLEI
K1024 EIVSRVSKRKLGRHV
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