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Protein Page:
APS (human)

Overview
APS an adapter protein involved in coupling immunoreceptors to Ras signaling. Acts as a negative regulator of cytokine signaling in collaboration with CBL. Binds to EPOR and suppresses EPO-induced STAT5 activation, possibly through a masking effect on STAT5 docking sites in EPOR. Suppresses PDGF-induced mitogenesis. Interacts with several proteins including KIT/c-KIT, SHC, GRB2, EPOR, CBL/c-CBL, PDGFR, VAV1 and VAV3. Interacts with SHC through its N-terminal region, with GRB2 via the phosphorylated C-terminus and with EPOR and KIT via its SH2 domain. Interacts with GRB2 after B-cell antigen receptor stimulation. Interacts with VAV3 via its PH domain, inducing cytoskeletal reorganization. Expressed in spleen, prostrate, testis, uterus, small intestine and skeletal muscle. Among haematopoietic cell lines, expressed exclusively in B-cells. Not expressed in most tumor cell lines. Tyrosine phosphorylated by JAK2, KIT and other kinases activated by B-cell receptor in response to stimulation with cytokines, IL3, IL5, PDGF, IGF1, IGF2, CSF2/GM-CSF and cross-linking of the B-cell receptor complex. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Cellular Component: cytoplasm; plasma membrane; cytosol
Molecular Function: protein binding; signal transducer activity; SH3/SH2 adaptor activity; JAK pathway signal transduction adaptor activity
Biological Process: regulation of JAK-STAT cascade; positive regulation of signal transduction; insulin receptor signaling pathway; blood coagulation; signal transduction
Reference #:  O14492 (UniProtKB)
Alt. Names/Synonyms: Adapter protein with pleckstrin homology and Src homology 2 domains; adaptor protein with pleckstrin homology and src homology 2 domains; APS; SH2 and PH domain-containing adapter protein APS; SH2B adapter protein 2; SH2B adaptor protein 2; SH2B2
Gene Symbols: SH2B2
Molecular weight: 67,738 Da
Basal Isoelectric point: 5.85  Predict pI for various phosphorylation states
CST Pathways:  Insulin Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

APS

Protein Structure Not Found.


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Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 3 S117-p APYGHSRsSEDVSTH
0 1 S136-p ARVRKGFsLRNMSLC
0 1 S141 GFsLRNMSLCVVDGV
0 2 S157-p DMWHRRAsPEPDAAA
0 1 S310-p GCVDPGDsEEDTELS
0 2 S596 QLSARSRSNSAERLL
1 1 S598 SARSRSNSAERLLEA
0 1 A599 ARSRSNSAERLLEAV
0 1 A607 ERLLEAVAATAAEEP
2 7 Y629-p ARAVENQySFY____
  mouse

 
S106-p ATYGHSRsSEDVSAH
S125 ARVRKGFSLRNMsLC
S130-p GFSLRNMsLCVVDGV
S146-p DLWHRRSsPEPDGGA
S303-p GCVDPGDsEEDTGLS
S586-p PLSAHSRsNstEHLL
S588-p SAHSRsNstEHLLDA
T589-p AHSRsNstEHLLDAA
S597-p EHLLDAAsGATEEPT
Y618-p ARAVENQySFY____
  rat

 
S106 ATYGHSRSSEDVSAH
S125 ARVRKGFSLRNMSLC
S130 GFSLRNMSLCVVDGV
S146 DLWHRRASPEPEGGA
S303 GCVDPGDSEEDTGLS
S586 PLSARSRSNsTEHLL
S588-p SARSRSNsTEHLLEA
T589 ARSRSNsTEHLLEAA
S597 EHLLEAASGATEEPA
Y618-p ARAVENQySFY____
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