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Protein Page:
Sin1 (human)

Overview
Sin1 a member of a conserved family of proteins that have an essential roles in signal transduction from yeast to mammals. An essential component of the mTORC2 complex, which consists of mTOR, LST8, Sin1, Protor (PRR5), and RICTOR, the Rapamycin Insensitive Companion of mTOR. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 regulates the phosphorylation of SGK1 S422 and PKCA S657. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Contains a Ras-binding (RBD) and a pleckstrin homology (PH) domains. Plays an important role in the SAPK signaling pathway by binding directly to both ATF-2 and p38. A genetic knockout of Sin1 abolished Akt-S473 phosphorylation and disrupts the RICTOR-mTOR interaction, but Akt-Thr308 phosphorylation is maintained. Six alternatively-spliced isoforms of the human protein have been reported. All isoforms except 4 can be incorporated into the the mTORC2 complex. Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold
Cellular Component: Golgi apparatus; cytoplasmic membrane-bound vesicle; plasma membrane; nucleus; cytosol
Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; protein binding; Ras GTPase binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylinositol-3,4-bisphosphate binding
Biological Process: epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; substantia nigra development; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; T cell costimulation; negative regulation of Ras protein signal transduction; innate immune response
Reference #:  Q9BPZ7 (UniProtKB)
Alt. Names/Synonyms: JC310; MAPKAP1; MEKK2-interacting protein 1; MGC2745; MIP1; Mitogen-activated protein kinase 2-associated protein 1; mitogen-activated protein kinase associated protein 1; mSIN1; ras inhibitor MGC2745; SAPK-interacting protein 1; SIN1; SIN1b; SIN1g; stress-activated map kinase interacting protein 1; Stress-activated map kinase-interacting protein 1; stress-activated protein kinase-interacting 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1
Gene Symbols: MAPKAP1
Molecular weight: 59,123 Da
Basal Isoelectric point: 7.24  Predict pI for various phosphorylation states
CST Pathways:  Insulin Receptor Signaling  |  mTOR Signaling  |  PI3K/Akt Signaling  |  Translation: eIF4E and p70S6K
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Sin1

Protein Structure Not Found.


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Sites Implicated In
cell growth, induced: S260‑p
protein stabilization: S260‑p

Modification Sites and Domains  

Modification Sites in Parent Protein, Orthologs, and Isoforms  
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S84-p DFGIRRRsNtAQRLE
1 11 T86-p GIRRRsNtAQRLERL
0 22 S128-p KSLFEKKsLKEKPPI
0 1 S185-p DVYLPLHssQDRLLP
0 1 S186-p VYLPLHssQDRLLPM
1 0 S260-p PIHKFGFsTLALVEK
0 6 S270-p ALVEKYSsPGLTSKE
0 1 Y497-p ASTARADyFAQKQRK
0 1 T509 QRKLNRRTsFsFQKE
0 64 S510-p RKLNRRTsFsFQKEK
0 4 S512-p LNRRTsFsFQKEKKS
  mouse

 
S84 DFGIRRRSNTAQRLE
T86 GIRRRSNTAQRLERL
S128-p KSLFEKKsLKEKPPS
S185 DVYLPLHSSQDRLLP
S186 VYLPLHSSQDRLLPM
S260 PIHKFGFSTLALVEK
S270-p ALVEKYSsPGLTSKE
Y497 ASTARADYLAQKQRK
T509-p QRKLNRRtsFsFQKE
S510-p RKLNRRtsFsFQKEK
S512-p LNRRtsFsFQKEKKS
  rat

 
S84 DFGIRRRSNTAQRLE
T86 GIRRRSNTAQRLERL
S128 KSLFEKKSLKEKPPS
S185 DVYLPLHSSQDRLLP
S186 VYLPLHSSQDRLLPM
S260 PIHKFGFSTLALVEK
S270 ALVEKYSSPGLTSKE
Y497 ASTARADYFAQKQRK
T509 QRKLNRRTsFSFQKE
S510-p RKLNRRTsFSFQKEK
S512 LNRRTsFSFQKEKKS
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