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Protein Page:
MASTL (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MASTL Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2). Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; MAST family
Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cleavage furrow
Molecular Function: protein serine/threonine kinase activity; protein phosphatase 2A binding; kinase activity; ATP binding
Biological Process: mitosis; regulation of cell cycle; mitotic cell cycle; negative regulation of protein phosphatase type 2A activity; G2/M transition of mitotic cell cycle; response to DNA damage stimulus; protein amino acid phosphorylation
Reference #:  Q96GX5 (UniProtKB)
Alt. Names/Synonyms: FLJ14813; greatwall; GW; GWL; MASTL; microtubule associated serine/threonine kinase-like; Microtubule-associated serine/threonine-protein kinase-like; RP11-85G18.2; THC2
Gene Symbols: MASTL
Molecular weight: 97,319 Da
Basal Isoelectric point: 5.67  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MASTL

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S31-p IAVPKPPsIEEFSIV
0 1 K39-ub IEEFSIVkPISRGAF
0 1 K66-ub YAVKVVKkADMINKN
0 1 K158-ub GIIHRDLkPDNMLIS
0 1 K179-ub LTDFGLSkVTLNRDI
2 3 T194-p NMMDILTtPSMAkPR
0 1 K199-ub LTtPSMAkPRQDYSR
1 1 T207-p PRQDYSRtPGQVLSL
0 1 S216-p GQVLSLIssLGFNtP
0 1 S217-p QVLSLIssLGFNtPI
0 2 T222-p IssLGFNtPIAEKNQ
0 1 T261-p SVDQKDTtPYssKLL
0 2 S264-p QKDTtPYssKLLKsC
0 1 S265-p KDTtPYssKLLKsCL
0 1 S270-p YssKLLKsCLEtVAs
0 1 T274-p LLKsCLEtVAsNPGM
0 2 S277-p sCLEtVAsNPGMPVK
0 1 S288-p MPVKCLTsNLLQsRK
0 2 S293-p LTsNLLQsRKRLATS
0 1 S303-p RLATSSAsSQSHTFI
0 1 S311-p SQSHTFIsSVESECH
0 1 S319-p SVESECHssPKWEKD
0 1 S320-p VESECHssPKWEKDC
0 1 S330-p WEKDCQEsDEALGPT
0 1 N353 KLCAKSANAIETKGF
0 1 K363 ETKGFNKKDLELALs
0 10 S370-p KDLELALsPIHNssA
0 2 S375-p ALsPIHNssALPtTG
0 2 S376-p LsPIHNssALPtTGR
0 1 T380-p HNssALPtTGRsCVN
0 1 S384-p ALPtTGRsCVNLAKK
0 1 S398 KCFSGEVSWEAVELD
0 2 S442-p EEHLGKRsLKRNFEL
0 4 S452-p RNFELVDssPCKKII
0 7 S453-p NFELVDssPCKKIIQ
0 4 S512-p NKENIVNsFtDKQQt
0 2 T514-p ENIVNsFtDKQQtPE
0 3 T519-p sFtDKQQtPEKLPIP
0 1 S550-p NSKRDYLsssFLCsD
0 1 S551-p SKRDYLsssFLCsDD
0 2 S552-p KRDYLsssFLCsDDD
0 2 S556-p LsssFLCsDDDRASK
0 1 T618-p TPDCQEKtSPKGVEN
0 4 S631-p ENPAVQEsNQKMLGP
0 1 K644-ac GPPLEVLkTLASKRN
0 1 K644-ub GPPLEVLkTLASKRN
0 5 S657-p RNAVAFRsFNsHINA
0 2 S660-p VAFRsFNsHINAsNN
0 3 S665-p FNsHINAsNNsEPsR
0 4 S668-p HINAsNNsEPsRMNM
0 2 S671-p AsNNsEPsRMNMTSL
0 1 - under review  
0 3 T710-p SCMPHQQtPNQIKSG
0 2 Y720-p QIKSGTPyRtPKsVR
0 3 T722-p KSGTPyRtPKsVRRG
0 3 S725-p TPyRtPKsVRRGVAP
0 2 T741-p DDGRILGtPDYLAPE
0 5 S875-p TAQHLTVsGFsL___
0 4 S878-p HLTVsGFsL______
  MASTL iso2  
S31 IAVPKPPSIEEFSIV
K39 IEEFSIVKPISRGAF
K66 YAVKVVKKADMINKN
K158 GIIHRDLKPDNMLIS
K179 LTDFGLSKVTLNRDI
T194 NMMDILTTPSMAKPR
K199 LTTPSMAKPRQDYSR
T207 PRQDYSRTPGQVLSL
S216 GQVLSLISSLGFNTP
S217 QVLSLISSLGFNTPI
T222 ISSLGFNTPIAEKNQ
T261 SVDQKDTTPYSSKLL
S264 QKDTTPYSSKLLKSC
S265 KDTTPYSSKLLKSCL
S270 YSSKLLKSCLETVAS
T274 LLKSCLETVASNPGM
S277 SCLETVASNPGMPVK
S288 MPVKCLTSNLLQSRK
S293 LTSNLLQSRKRLATS
S303 RLATSSASSQSHTFI
S311 SQSHTFISSVESECH
S319 SVESECHSSPKWEKD
S320 VESECHSSPKWEKDC
S330 WEKDCQESDEALGPT
N353 KLCAKSANAIETKGF
K363 ETKGFNKKDLELALS
S370 KDLELALSPIHNSSA
S375 ALSPIHNSSALPTTG
S376 LSPIHNSSALPTTGR
T380 HNSSALPTTGRSCVN
S384 ALPTTGRSCVNLAKK
S398 KCFSGEVSWEAVELD
S442 EEHLGKRSLKRNFEL
S452 RNFELVDSSPCKKII
S453 NFELVDSSPCKKIIQ
S512 NKENIVNSFTDKQQT
T514 ENIVNSFTDKQQTPE
T519 SFTDKQQTPEKLPIP
S550 NSKRDYLSSSFLCSD
S551 SKRDYLSSSFLCSDD
S552 KRDYLSSSFLCSDDD
S556 LSSSFLCSDDDRASK
T618 TPDCQEKTSPKGVEN
S631 ENPAVQESNQKMLGP
K644 GPPLEVLKTLASKRN
K644 GPPLEVLKTLASKRN
S657 RNAVAFRSFNSHINA
S660 VAFRSFNSHINASNN
S665 FNSHINASNNSEPSR
S668 HINASNNSEPSRMNM
S671 ASNNSEPSRMNMTSL
S703-p TPTQKRRsCMPHQtP
T709-p RsCMPHQtPNQIKSG
Y719 QIKSGTPYRTPKSVR
T721 KSGTPYRTPKSVRRG
S724 TPYRTPKSVRRGVAP
T740 DDGRILGTPDYLAPE
S836 TAQHLTVSGFSL___
S839 HLTVSGFSL______
  mouse

 
S30 IPVPRPPSIEEFTIV
K38 IEEFTIVKPISRGAF
K65 YAVKVVKKADMINKN
K157 GIIHRDLKPDNMLIS
K178 LTDFGLSKVTLNRDI
T193-p NMMDILTtPSMSKPK
K198 LTtPSMSKPKQDYSR
T206-p PKQDYSRtPGQVLSL
S215 GQVLSLISSLGFFTP
S216 QVLSLISSLGFFTPV
T221 ISSLGFFTPVGEKDQ
T259 SVDQKEPTSYSSKLL
S262 QKEPTSYSSKLLKSC
S263 KEPTSYSSKLLKSCF
S268 YSSKLLKSCFETLSS
T272 LLKSCFETLSSNPEI
S275 SCFETLSSNPEIPVK
S286 IPVKCLTSNLLQCRK
C291 LTSNLLQCRKRLGTS
S301 RLGTSSTSSQSHTFV
S309 SQSHTFVSSVESECH
S317 SVESECHSNPKWERD
N318 VESECHSNPKWERDC
S327 KWERDCQSTESSGCA
S349-p MLYAKSTsAIKTKtE
T355-p TsAIKTKtELELALS
S362 tELELALSPIHDSsA
S367 ALSPIHDSsAIPAAG
S368-p LSPIHDSsAIPAAGS
A372 HDSsAIPAAGSNQVT
N376 AIPAAGSNQVTLPRK
S389-p RKCFREIsWEARDPD
N431 EEHLGKRNYKRNFHL
S441 RNFHLVDSSPCQEIM
S442 NFHLVDSSPCQEIMQ
Y502 NKENIVTYLTDRQTP
T504 ENIVTYLTDRQTPEK
T508 TYLTDRQTPEKLHIP
S539 SSKKDCLSSNSVCSD
S540 SKKDCLSSNSVCSDE
N541 KKDCLSSNSVCSDED
S545 LSSNSVCSDEDRALK
S606 LPKCQENSLPQDDCH
S619 CHACIQDSSQVSAHP
K631 AHPSKAPKALTSKIN
K631 AHPSKAPKALTSKIN
S644 INVVAFRSFNSHINA
S647 VAFRSFNSHINASTN
S652 FNSHINASTNSEPSK
S655 HINASTNSEPSKISI
S658 ASTNSEPSKISITSL
H690 SPTEKGRHYTSHQTP
T696 RHYTSHQTPNQVKLG
Y706 QVKLGTSYRTPKSVR
T708 KLGTSYRTPKSVRRG
S711 TSYRTPKSVRRGAAP
T727 DDGRILGTPDYLAPE
S861-p NAQHLTIsGFSL___
S864 HLTIsGFSL______
  rat

 
S30 IPVPRPPSIEEFTIV
K38 IEEFTIVKPISRGAF
K65 YAVKVVKKADMINKN
K157 GIIHRDLKPDNMLIS
K178 LTDFGLSKVTLNRDI
T193 NMMDILTTPSMSKPK
K198 LTTPSMSKPKQDYSR
T206 PKQDYSRTPGQVLSL
S215 GQVLSLISSLGFFTP
S216 QVLSLISSLGFFTPV
T221 ISSLGFFTPVGEKDQ
I259 SIDQKEPISYSSKLL
S262 QKEPISYSSKLLKSC
S263 KEPISYSSKLLKSCF
S268 YSSKLLKSCFETLAS
T272 LLKSCFETLASNPEI
S275 SCFETLASNPEIPVK
S286 IPVKCLTSNLLQCRK
C291 LTSNLLQCRKRLGTS
S301 RLGTSSTSSQSHTFV
S309 SQSHTFVSSVESECH
S317 SVESECHSSPKWEKD
S318 VESECHSSPKWEKDC
S327 KWEKDCQSAKPSSTM
S349 MPYAKSVSAIETKTD
T355 VSAIETKTDLELALS
S362 TDLELALSPIHDSSN
S367 ALSPIHDSSNIPPTG
S368 LSPIHDSSNIPPTGR
- gap
N376 NIPPTGRNYMTLPRK
S390 KCFSREISWEAREPD
N432 EEHLCKRNCKRNFHL
S442 RNFHLVDSSPCQEII
S443 NFHLVDSSPCQEIIQ
Y503 NKENTVTYLTDRRTP
T505 ENTVTYLTDRRTPEE
T509 TYLTDRRTPEELHIP
H540 SSKKDCLHSNSVCSD
S541 SKKDCLHSNSVCSDE
N542 KKDCLHSNSVCSDED
S546 LHSNSVCSDEDRAPK
S607 LPKCQENSLPKDDWH
S620 WHASIQDSNQMLAPP
K632 APPSKAPKTFASKTN
K632 APPSKAPKTFASKTN
S645 TNVVAFRSFSSHINA
S648 VAFRSFSSHINASSN
S653 FSSHINASSNSEPSK
S656 HINASSNSEPSKVST
S659 ASSNSEPSKVSTTSL
A691 TPTEKGRACVSHQTP
T697 RACVSHQTPSQVKLG
Y707 QVKLGTSYRTPKSVR
T709 KLGTSYRTPKSVRRG
S712 TSYRTPKSVRRGPPP
T728 DDGRILGTPDYLAPE
S862 NAQHLTISGFSL___
S865 HLTISGFSL______
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