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Protein Page:
PIAS3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PIAS3 Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Involved in regulating STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Monomer. Binds SUMO1 and UBE2I. Interacts with BCL11A, HMGA2, IRF1, MITF and NCOA2. Interacts with STAT5; the interaction occurs on stimulation by PRL. Interacts with GFI1; the interaction relieves the inhibitory effect of PIAS3 on STAT3- mediated transcriptional activity. Interacts with AR, PLAG1 and ZFHX3. Interacts with STAT3; the interaction occurs on stimulation by IL6, CNTF or OSM and inhibits the DNA binding activity of STAT3. By dihydrotestosterone (DHT) in prostate cancer cells. Isoform 1 is expressed in most tissues except thymus and small intestine. Isoform 3 is expressed only in brain, heart, thymus, muscle, lung, testis, lactating breast and embryonic stem cells. Belongs to the PIAS family. Note: This description may include information from UniProtKB.
Protein type: SUMO conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-
Cellular Component: cytoplasm; dendrite; synapse; nucleus
Molecular Function: protein C-terminus binding; protein binding; enzyme binding; potassium channel regulator activity; nucleic acid binding; zinc ion binding; protein N-terminus binding; SUMO ligase activity
Biological Process: positive regulation of protein sumoylation; protein sumoylation; transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to hormone stimulus; positive regulation of membrane potential
Reference #:  Q9Y6X2 (UniProtKB)
Alt. Names/Synonyms: E3 SUMO-protein ligase PIAS3; FLJ14651; PIAS3; Protein inhibitor of activated STAT protein 3; protein inhibitor of activated STAT, 3; zinc finger, MIZ-type containing 5; ZMIZ5
Gene Symbols: PIAS3
Molecular weight: 68,017 Da
Basal Isoelectric point: 7.81  Predict pI for various phosphorylation states
CST Pathways:  IL6 Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PIAS3

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K307-ub SRALIKEkLTADPDS
0 1 S404 IQFMEDGSWCPMKPK
0 1 A602 SIVAPGGALREGHGG
  mouse

 
K307 SRALIKEKLTADPDS
S404-p IQFMEDGsWCPMKPK
S602 SIVAPGSSLREGHGG
  rat

 
K307 SRALIKEKLTADPDS
S404 IQFMEDGSWCPMKPK
S602-p SIVAPGSsLREGHGG
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