Oct4 (human)

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Protein Page:

Oct4 (human)

p	Phosphorylation
a	Acetylation
m	Methylation
m1	Mono-methylation
m2	Di-methylation
m3	Tri-methylation
u	Ubiquitination
s	Sumoylation
n	Neddylation
gl	O-GlcNAc
ga	O-GalNAc
h	Palmitoylation
ad	Adenylylation
sn	S-Nitrosylation
ca	Caspase cleavage

Overview

Oct4 Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.

Protein type: DNA binding protein; Transcription factor

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; anatomical structure morphogenesis; blastocyst development; regulation of transcription, DNA-dependent; regulation of asymmetric cell division; somatic stem cell maintenance; regulation of gene expression; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter

Reference #: Q01860 (UniProtKB)

Alt. Names/Synonyms: MGC22487; Oct-3; Oct-4; OCT3; OCT3A; OCT4; OCT4A; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor-3; OTF-3; OTF3; OTF4; PO5F1; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1

Gene Symbols: POU5F1

Molecular weight: 38,571 Da

Basal Isoelectric point: 5.69 Predict pI for various phosphorylation states

CST Pathways: ESC Pluripotency and Differentiation

Protein-Specific Antibodies or siRNAs from Cell Signaling Technology^®

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	Oct4

Sites Implicated In

transcription, altered: S12‑p, S111‑p
transcription, inhibited: T235‑p, S236‑p
inhibition: S111‑p
intracellular localization: S111‑p
molecular association, regulation: S12‑p, T235‑p, S236‑p
protein degradation: S111‑p
protein stabilization: S12‑p
ubiquitination: S111‑p

Modification Sites and Domains

Show Modification Legend

Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend

Show Multiple Sequence Alignment

SS SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.		human ► Hide Isoforms

1	0	S12-p	LASDFAFsPPPGGGG
0	1	T92-p	VPQGGLEtsQPEGEA
0	1	S93-p	PQGGLEtsQPEGEAG
0	1	S105-p	EAGVGVEsNsDGAsP
0	1	S107-p	GVGVEsNsDGAsPEP
1	2	S111-p	EsNsDGAsPEPCtVt
0	1	T116-p	GAsPEPCtVtPGAVk
0	1	T118-p	sPEPCtVtPGAVkLE
0	1	K123	tVtPGAVKLEKEKLE
3	0	K123-s	tVtPGAVkLEKEKLE
0	1	K140-u	PEESQDIkALQKELE
0	1	K144	QDIkALQKELEQFAk
0	3	K151-u	KELEQFAkLLKQKRI
0	1	T163-p	KRITLGYtQADVGLT
0	1	S180-p	VLFGKVFsQTTICRF
0	3	K206-u	KLRPLLQkWVEEADN
0	4	K222	ENLQEICKAETLVQA
1	2	T235-p	QARKRKRtsIENRVR
1	2	S236-p	ARKRKRtsIENRVRG
0	2	S288-p	RRQKGKRssSDYAQR
0	2	S289-p	RQKGKRssSDYAQRE
0	1	T352-p	FPPVSVTtLGsPMHs
0	1	S355-p	VSVTtLGsPMHsN__
0	1	S359-p	tLGsPMHsN______

	Oct4 iso2

-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
K45	KIQSQDIKALQKELE
K49	QDIKALQKELEQFAK
K56	KELEQFAKLLKQKRI
T68	KRITLGYTQADVGLT
S85	VLFGKVFSQTTICRF
K111	KLRPLLQKWVEEADN
K127	ENLQEICKAETLVQA
T140	QARKRKRTSIENRVR
S141	ARKRKRTSIENRVRG
S193	RRQKGKRSSSDYAQR
S194	RQKGKRSSSDYAQRE
T257	FPPVSVTTLGSPMHS
S260	VSVTTLGSPMHSN__
S264	TLGSPMHSN______

	mouse ► Hide Isoforms

S12	LASDFAFSPPPGGGD
T87	VPQVGVETLQPEGQA
L88	PQVGVETLQPEGQAG
S100	QAGARVESNSEGTSS
S102	GARVESNSEGTSSEP
S106	ESNSEGTSSEPCADR
A111	GTSSEPCADRPNAVk
R113	SSEPCADRPNAVkLE
K118-u	ADRPNAVkLEKVEPT
K118-s	ADRPNAVkLEKVEPT
K133	PEESQDMKALQkELE
K137-u	QDMKALQkELEQFAk
K144-u	kELEQFAkLLKQKRI
T156	KRITLGYTQADVGLT
S173	VLFGKVFSQTTICRF
K199-u	KLRPLLEkWVEEADN
K215-u	ENLQEICkSETLVQA
T228-p	QARKRKRtSIENRVR
S229	ARKRKRtSIENRVRW
S281	RRQKGKRSSIEYSQR
S282	RQKGKRSSIEYSQRE
A344	FPSVPVTALGSPMHS
S347	VPVTALGSPMHSN__
S351	ALGSPMHSN______

	Oct4 iso2

-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
K2	______MKALQKELE
K6	__MKALQKELEQFAK
K13	KELEQFAKLLKQKRI
T25	KRITLGYTQADVGLT
S42	VLFGKVFSQTTICRF
K68	KLRPLLEKWVEEADN
K84	ENLQEICKSETLVQA
T97	QARKRKRTSIENRVR
S98	ARKRKRTSIENRVRW
S150	RRQKGKRSSIEYSQR
S151	RQKGKRSSIEYSQRE
A213	FPSVPVTALGSPMHS
S216	VPVTALGSPMHSN__
S220	ALGSPMHSN______

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