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Protein Page:
Oct4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Oct4 Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus
Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; blastocyst development; anatomical structure morphogenesis; regulation of transcription, DNA-dependent; regulation of asymmetric cell division; somatic stem cell maintenance; regulation of gene expression; endodermal cell fate specification; response to wounding; positive regulation of transcription from RNA polymerase II promoter; mRNA transcription from RNA polymerase II promoter
Reference #:  Q01860 (UniProtKB)
Alt. Names/Synonyms: MGC22487; Oct-3; Oct-4; OCT3; OCT3A; OCT4; OCT4A; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor-3; OTF-3; OTF3; OTF4; PO5F1; POU class 5 homeobox 1; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1
Gene Symbols: POU5F1
Molecular weight: 38,571 Da
Basal Isoelectric point: 5.69  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Oct4

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: S12‑p, S111‑p
transcription, inhibited: T235‑p, S236‑p
activity, inhibited: S111‑p
intracellular localization: S111‑p
molecular association, regulation: S12‑p, T235‑p, S236‑p
protein degradation: S111‑p
protein stabilization: S12‑p
ubiquitination: S111‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
1 0 S12-p LASDFAFsPPPGGGG
0 1 T92-p VPQGGLEtsQPEGEA
0 1 S93-p PQGGLEtsQPEGEAG
0 1 S105-p EAGVGVEsNsDGAsP
0 1 S107-p GVGVEsNsDGAsPEP
1 2 S111-p EsNsDGAsPEPCtVt
0 1 T116-p GAsPEPCtVtPGAVk
0 1 T118-p sPEPCtVtPGAVkLE
0 1 K123 tVtPGAVKLEKEKLE
4 0 K123-sm tVtPGAVkLEKEKLE
0 1 K140-ub PEESQDIkALQKELE
0 1 K144 QDIkALQKELEQFAk
0 3 K151-ub KELEQFAkLLKQKRI
0 1 T163-p KRITLGYtQADVGLT
0 1 S180-p VLFGKVFsQTTICRF
0 3 K206-ub KLRPLLQkWVEEADN
0 4 K222 ENLQEICKAETLVQA
1 2 T235-p QARKRKRtsIENRVR
1 2 S236-p ARKRKRtsIENRVRG
0 2 S288-p RRQKGKRssSDYAQR
0 2 S289-p RQKGKRssSDYAQRE
0 1 T352-p FPPVSVTtLGsPMHs
0 1 S355-p VSVTtLGsPMHsN__
0 1 S359-p tLGsPMHsN______
  Oct4 iso2  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K45 KIQSQDIKALQKELE
K49 QDIKALQKELEQFAK
K56 KELEQFAKLLKQKRI
T68 KRITLGYTQADVGLT
S85 VLFGKVFSQTTICRF
K111 KLRPLLQKWVEEADN
K127 ENLQEICKAETLVQA
T140 QARKRKRTSIENRVR
S141 ARKRKRTSIENRVRG
S193 RRQKGKRSSSDYAQR
S194 RQKGKRSSSDYAQRE
T257 FPPVSVTTLGSPMHS
S260 VSVTTLGSPMHSN__
S264 TLGSPMHSN______
  mouse

► Hide Isoforms
 
S12 LASDFAFSPPPGGGD
T87 VPQVGVETLQPEGQA
L88 PQVGVETLQPEGQAG
S100 QAGARVESNSEGTSS
S102 GARVESNSEGTSSEP
S106 ESNSEGTSSEPCADR
A111 GTSSEPCADRPNAVk
R113 SSEPCADRPNAVkLE
K118-ub ADRPNAVkLEKVEPT
K118-sm ADRPNAVkLEKVEPT
K133 PEESQDMKALQkELE
K137-ub QDMKALQkELEQFAk
K144-ub kELEQFAkLLKQKRI
T156 KRITLGYTQADVGLT
S173 VLFGKVFSQTTICRF
K199-ub KLRPLLEkWVEEADN
K215-ub ENLQEICkSETLVQA
T228-p QARKRKRtSIENRVR
S229 ARKRKRtSIENRVRW
S281 RRQKGKRSSIEYSQR
S282 RQKGKRSSIEYSQRE
A344 FPSVPVTALGSPMHS
S347 VPVTALGSPMHSN__
S351 ALGSPMHSN______
  Oct4 iso2  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
K2 ______MKALQKELE
K6 __MKALQKELEQFAK
K13 KELEQFAKLLKQKRI
T25 KRITLGYTQADVGLT
S42 VLFGKVFSQTTICRF
K68 KLRPLLEKWVEEADN
K84 ENLQEICKSETLVQA
T97 QARKRKRTSIENRVR
S98 ARKRKRTSIENRVRW
S150 RRQKGKRSSIEYSQR
S151 RQKGKRSSIEYSQRE
A213 FPSVPVTALGSPMHS
S216 VPVTALGSPMHSN__
S220 ALGSPMHSN______
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