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NANOG (human)

Overview NANOG Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Transcription factor; DNA binding protein; Cell development/differentiation Cellular Component: nucleolus; nucleus Molecular Function: DNA binding; sequence-specific DNA binding; transcription corepressor activity; transcription factor activity Biological Process: cell proliferation; embryonic development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; regulation of gene expression; somatic stem cell maintenance; regulation of cell differentiation; endodermal cell fate specification; positive regulation of transcription from RNA polymerase II promoter; cell differentiation Reference #:  Q9H9S0 (UniProtKB) Alt. Names/Synonyms: hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox Gene Symbols: NANOG Molecular weight: 34,620 Da Basal Isoelectric point: 6.32  Predict pI for various phosphorylation states CST Pathways:  ESC Pluripotency and Differentiation Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below NANOG

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	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
1	0		Y35-p	ICGPEENyPSLQMSS
0	1		Y119	DRFQRQKYLSLQQMQ
0	1		S135	LSNILNLSYKQVKTW
0	2		K137	NILNLSYKQVKTWFQ
0	2		K168	NSNGVTQKASAPTyP
1	0		Y174-p	QKASAPTyPSLYSSY
0	1		T286-p	QTTRYFStPQtMDLF
0	1		T289-p	RYFStPQtMDLFLNY

	mouse
Y35	AVFHPENYSCLQGSA
Y120	DRFQKQKYLSLQQMQ
S136	LSSILNLSYkQVKTW
K138-u	SILNLSYkQVKTWFQ
K169-u	TSNGLIQkGSAPVEY
Y176	kGSAPVEYPSIHCSY
T287	ESHAHFSTPQALELF
A290	AHFSTPQALELFLNY

	rat
Y36	VHLPEENYSCLQVSA
Y122-p	DRFQRQRyLSLQQMQ
S138-p	LSTILNLsYKQVKTW
K140	TILNLsYKQVKTWFQ
K171	TSNGLTQKGSAPVEY
Y178	KGSAPVEYPSIHCSY
T294	ESQAHFSTPQALELF
A297	AHFSTPQALELFLNY

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