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Protein Page:
NANOG (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NANOG Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, promotes cells to enter into S phase and proliferation. Interacts with SMAD1 and SALL4. Expressed in testicular carcinoma and derived germ cell tumors. Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. Belongs to the Nanog homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein; Cell development/differentiation
Chromosomal Location of Human Ortholog: 12p13.31
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: DNA binding; chromatin binding; transcription corepressor activity; transcription factor activity
Biological Process: gonad development; response to retinoic acid; transcription, DNA-dependent; somatic stem cell maintenance; stem cell division; endodermal cell fate specification; negative regulation of transcription from RNA polymerase II promoter; positive regulation of mitotic cell cycle; embryonic pattern specification; mesodermal cell fate commitment; negative regulation of cell fate commitment; negative regulation of BMP signaling pathway; cell proliferation; embryonic development; regulation of transcription, DNA-dependent; regulation of gene expression; regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; cell differentiation
Reference #:  Q9H9S0 (UniProtKB)
Alt. Names/Synonyms: hNanog; Homeobox protein NANOG; Homeobox transcription factor Nanog; homeobox transcription factor Nanog-delta 48; NANOG; Nanog homeobox
Gene Symbols: NANOG
Molecular weight: 34,620 Da
Basal Isoelectric point: 6.32  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NANOG

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
1 0 Y35-p ICGPEENyPSLQMSS
1 0 T50-p AEMPHTEtVsPLPss
1 0 S52-p MPHTEtVsPLPssMD
1 0 S56-p EtVsPLPssMDLLIQ
1 0 S57-p tVsPLPssMDLLIQD
1 0 S65-p MDLLIQDsPDSStsP
1 0 T70-p QDsPDSStsPKGKQP
1 0 S71-p DsPDSStsPKGKQPt
1 1 T78-p sPKGKQPtsAEKSVA
2 0 S79-p PKGKQPtsAEKSVAK
0 1 Y119 DRFQRQKYLSLQQMQ
1 1 S135-p LSNILNLsYKQVKTW
0 2 K137 NILNLsYKQVKTWFQ
0 2 K168 NSNGVTQKASAPTyP
1 0 Y174-p QKASAPTyPSLYSSY
1 0 T200-p LPMWSNQtWNNSTWS
1 0 S258-p CMQFQPNsPASDLEA
1 0 T280-p GLNVIQQtTRYFStP
0 1 T286-p QtTRYFStPQtMDLF
0 1 T289-p RYFStPQtMDLFLNY
  mouse

 
Y35 AVFHPENYSCLQGSA
A50 TEMLCTEAASPRPSS
S52 MLCTEAASPRPSSED
S56 EAASPRPSSEDLPLQ
S57 AASPRPSSEDLPLQG
S65 EDLPLQGSPDSSTSP
T70 QGSPDSSTSPKQKLS
S71 GSPDSSTSPKQKLSs
S78-p SPKQKLSsPEADKGP
P79 PKQKLSsPEADKGPE
Y120 DRFQKQKYLSLQQMQ
S136 LSSILNLSYkQVKTW
K138-ub SILNLSYkQVKTWFQ
K169-ub TSNGLIQkGSAPVEY
Y176 kGSAPVEYPSIHCSY
T202 LSMWGSQTWTNPTWS
S266 YIQLQQNSSASDLEV
S281 NLEATRESHAHFSTP
T287 ESHAHFSTPQALELF
A290 AHFSTPQALELFLNY
  rat

 
Y36 VHLPEENYSCLQVSA
T51 TEMLCTETASPPPSS
S53 MLCTETASPPPSSGD
S57 ETASPPPSSGDLPLQ
S58 TASPPPSSGDLPLQD
S66 GDLPLQDSPDSSSNP
S71 QDSPDSSSNPKLKLS
N72 DSPDSSSNPKLKLSG
G79 NPKLKLSGPEADEGP
P80 PKLKLSGPEADEGPE
Y122-p DRFQRQRyLSLQQMQ
S138-p LSTILNLsYKQVKTW
K140 TILNLsYKQVKTWFQ
K171 TSNGLTQKGSAPVEY
Y178 KGSAPVEYPSIHCSY
T204 LPVWGSQTWTNPTWN
F273 YVPLQQNFSASDLEA
S288 NLEATRESQAHFSTP
T294 ESQAHFSTPQALELF
A297 AHFSTPQALELFLNY
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