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Protein Page:
ISG15 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
ISG15 Ubiquitin-like protein that is conjugated to intracellular target proteins after IFN-alpha or IFN-beta stimulation. Its enzymatic pathway is partially distinct from that of ubiquitin, differing in substrate specificity and interaction with ligating enzymes. ISG15 conjugation pathway uses a dedicated E1 enzyme, but seems to converge with the Ub conjugation pathway at the level of a specific E2 enzyme. Targets include STAT1, SERPINA3G/SPI2A, JAK1, MAPK3/ERK1, PLCG1, EIF2AK2/PKR, MX1/MxA, and RIG-1. Deconjugated by USP18/UBP43. Shows specific chemotactic activity towards neutrophils and activates them to induce release of eosinophil chemotactic factors. May serve as a trans-acting binding factor directing the association of ligated target proteins to intermediate filaments. May also be involved in autocrine, paracrine and endocrine mechanisms, as in cell-to-cell signaling, possibly partly by inducing IFN-gamma secretion by monocytes and macrophages. Seems to display antiviral activity during viral infections. Interacts with, and is conjugated to its targets by the UBE1L (E1 enzyme) and UBE2E2 (E2 enzyme) (Probable). Interaction with influenza B NS1 protein inhibits this conjugation. By type I interferons. Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin-like modifier
Chromosomal Location of Human Ortholog: 1p36.33
Cellular Component: extracellular region; cytosol
Molecular Function: protein tag; protein binding
Biological Process: modification-dependent protein catabolic process; ISG15-protein conjugation; viral reproduction; negative regulation of viral genome replication; regulation of interferon-gamma production; positive regulation of erythrocyte differentiation; cytokine and chemokine mediated signaling pathway; defense response to bacterium; innate immune response; negative regulation of protein ubiquitination; negative regulation of interferon type I production; defense response to virus
Reference #:  P05161 (UniProtKB)
Alt. Names/Synonyms: G1P2; hUCRP; IFI15; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein, 15 kDa; ISG15; ISG15 ubiquitin-like modifier; Ubiquitin cross-reactive protein; UCRP
Gene Symbols: ISG15
Molecular weight: 17,888 Da
Basal Isoelectric point: 6.83  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ISG15

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T6 __MGWDLTVKMLAGN
0 1 S22 FQVSLSSSMSVSELK
0 1 S26 LSSSMSVSELKAQIT
0 2 K35-ub LKAQITQkIGVHAFQ
0 1 K108 TQTVAHLKQQVSGLE
0 2 K143 PLGEYGLKPLSTVFM
0 2 M150 KPLSTVFMNLRLRGG
  mouse

 
K6-ub __MAWDLkVKMLGGN
S22 FLVSVTNSMTVSELK
S26 VTNSMTVSELKKQIA
K35-ub LKKQIAQkIGVPAFQ
K106-ub TQTVDTLkKKVSQRE
K141-ub LLGEYGLkPQCTVIk
K148-ub kPQCTVIkHLRLRGG
  rat

 
K6 __MTWNLKVKMLGGK
S22-p FLVSMTNsMMLsELK
S26-p MTNsMMLsELKKQVA
K35 LKKQVAQKSGVPAFQ
M107 TQTVEVLMRQVSQHE
T142 PLGEYGLTPHCTVIM
M149 TPHCTVIMNLRLRGG
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