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Protein Page:
AP2M1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
AP2M1 a protein of the adaptor complexes medium subunit family. A subunit of the heterotetrameric coat assembly protein complex 2 (AP2) which links clathrin to receptors in coated vesicles. Interacts with the cytoplasmic tails of membrane proteins and polyphosphoinositide-containing lipids. Is not required for clathrin-coated vesicle formation at the plasma membrane, but that it is one of several endocytic adaptors required for the uptake of certain cargo proteins. Is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. AP-2 is a heterotetramer composed of two large chains (alpha and beta), a medium chain (AP50) and a small chain (AP17). Note: This description may include information from UniProtKB.
Protein type: Adaptor/scaffold; Vesicle protein
Cellular Component: clathrin adaptor complex; mitochondrion; lysosomal membrane; plasma membrane; coated pit; cytosol
Molecular Function: protein binding; transporter activity; lipid binding
Biological Process: negative regulation of epidermal growth factor receptor signaling pathway; epidermal growth factor receptor signaling pathway; synaptic transmission; intracellular protein transport; axon guidance; nerve growth factor receptor signaling pathway; viral reproduction; antigen processing and presentation of exogenous peptide antigen via MHC class II; endocytosis; regulation of defense response to virus by virus
Reference #:  Q96CW1 (UniProtKB)
Alt. Names/Synonyms: Adapter-related protein complex 2 mu subunit; Adaptin-mu2; Adaptor protein complex AP-2 subunit mu; adaptor-related protein complex 2, mu 1 subunit; AP-2 complex subunit mu; AP-2 mu 2 chain; AP-2 mu chain; AP-2 mu2; AP2M1; AP50; CLAPM1; clathrin adaptor complex AP2, mu subunit; Clathrin assembly protein complex 2 medium chain; clathrin coat adaptor protein AP50; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; HA2 50 kDa subunit; KIAA0109; mu2; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein
Gene Symbols: AP2M1
Molecular weight: 49,655 Da
Basal Isoelectric point: 9.57  Predict pI for various phosphorylation states
Select Structure to View Below

AP2M1

Protein Structure Not Found.


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Sites Implicated In
intracellular localization: T156‑p
molecular association, regulation: T156‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 3 S45-p HARQQVRsPVTNIAR
0 1 S61 SFFHVKRSNIWLAAV
0 1 T134 GALKTFITQQGIkSQ
0 2 K139-ub FITQQGIkSQHQTKE
0 1 K145 IkSQHQTKEEQSQIt
0 1 T152-p KEEQSQItsQVtGQI
0 1 S153-p EEQSQItsQVtGQIG
5 42 T156-p SQItsQVtGQIGWRR
0 1 K167 GWRREGIKYRRNELF
0 2 K203-ac VSGRVVMkSYLSGMP
0 2 Y205 GRVVMkSYLSGMPEC
0 1 K219 CKFGMNDKIVIEkQG
0 3 K224-ub NDKIVIEkQGkGTAD
0 2 K227-ub IVIEkQGkGTADETS
0 2 K235-ub GTADETSksGkQsIA
0 1 S236-p TADETSksGkQsIAI
0 11 K238-ub DETSksGkQsIAIDD
0 1 S240-p TSksGkQsIAIDDCT
0 1 K256 HQCVRLSKFDsERsI
0 4 K256-ub HQCVRLSkFDsERsI
0 1 S259-p VRLSkFDsERsISFI
0 1 S262-p SkFDsERsISFIPPD
0 2 K281-ub LMRYRTTkDIILPFR
0 1 K304-ac GRTKLEVkVVIkSNF
0 1 K308-ac LEVkVVIkSNFkPSL
0 4 K312-ub VVIkSNFkPSLLAQK
0 3 S314 IkSNFkPSLLAQKIE
0 2 K339-ub GVQVICMkGKAKykA
0 1 Y344-p CMkGKAKykASENAI
0 2 K345-ub MkGKAKykASENAIV
0 1 S347 GKAKykASENAIVWK
0 1 K354 SENAIVWKIKRMAGM
0 1 K354 SENAIVWKIKRMAGM
0 8 K378-ub ELLPTNDkkkWARPP
0 5 K379-ub LLPTNDkkkWARPPI
0 4 K380-ub LPTNDkkkWARPPIS
0 2 K400 PFAPSGLKVRYLKVF
0 2 K405 GLKVRYLKVFEPkLN
0 3 K410-ub YLKVFEPkLNYSDHD
0 2 K420-ub YSDHDVIkWVRYIGR
0 1 Y431-p YIGRSGIyEtRC___
0 1 T433-p GRSGIyEtRC_____
3843 : Phospho-AP2M1 (Thr156) Antibody
7399 : Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb
  AP2M1 iso2  
S45 HARQQVRSPVTNIAR
S61 SFFHVKRSNIWLAAV
T134-p GALKTFItQQGIKSQ
K139 FItQQGIKSQTKEEQ
K143 QGIKSQTKEEQSQIT
T150 KEEQSQITSQVtGQI
S151 EEQSQITSQVtGQIG
T154-p SQITSQVtGQIGWRR
K165 GWRREGIKYRRNELF
K201 VSGRVVMKSYLSGMP
Y203 GRVVMKSYLSGMPEC
K217 CKFGMNDKIVIEKQG
K222 NDKIVIEKQGKGTAD
K225 IVIEKQGKGTADETS
K233 GTADETSKSGKQSIA
S234 TADETSKSGKQSIAI
K236 DETSKSGKQSIAIDD
S238 TSKSGKQSIAIDDCT
K254 HQCVRLSKFDSERSI
K254 HQCVRLSKFDSERSI
S257 VRLSKFDSERSISFI
S260 SKFDSERSISFIPPD
K279 LMRYRTTKDIILPFR
K302 GRTKLEVKVVIKSNF
K306 LEVKVVIKSNFKPSL
K310 VVIKSNFKPSLLAQK
S312 IKSNFKPSLLAQKIE
K337 GVQVICMKGKAKYKA
Y342 CMKGKAKYKASENAI
K343 MKGKAKYKASENAIV
S345 GKAKYKASENAIVWK
K352 SENAIVWKIKRMAGM
K352 SENAIVWKIKRMAGM
K376 ELLPTNDKKKWARPP
K377 LLPTNDKKKWARPPI
K378 LPTNDKKKWARPPIS
K398 PFAPSGLKVRYLKVF
K403 GLKVRYLKVFEPKLN
K408 YLKVFEPKLNYSDHD
K418 YSDHDVIKWVRYIGR
Y429 YIGRSGIYETRC___
T431 GRSGIYETRC_____
3843 : Phospho-AP2M1 (Thr156) Antibody
7399 : Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb
  mouse

► Hide Isoforms
 
S45-p HARQQVRsPVTNIAR
S61-p SFFHVKRsNIWLAAV
T134 GALKTFITQQGIkSQ
K139-ub FITQQGIkSQHQTkE
K145-ub IkSQHQTkEEQSQIt
T152-p kEEQSQItsQVtGQI
S153-p EEQSQItsQVtGQIG
T156-p SQItsQVtGQIGWRR
K167-ac GWRREGIkYRRNELF
K203 VSGRVVMKSYLSGMP
Y205 GRVVMKSYLSGMPEC
K219-ub CKFGMNDkIVIEkQG
K224-ub NDkIVIEkQGKGTAD
K227 IVIEkQGKGTADETS
K235 GTADETSKSGkQsIA
S236 TADETSKSGkQsIAI
K238-ub DETSKSGkQsIAIDD
S240-p TSKSGkQsIAIDDCT
K256-ac HQCVRLSkFDSERSI
K256-ub HQCVRLSkFDSERSI
S259 VRLSkFDSERSISFI
S262 SkFDSERSISFIPPD
K281-ub LMRYRTTkDIILPFR
K304 GRTKLEVKVVIKSNF
K308 LEVKVVIKSNFkPsL
K312-ub VVIKSNFkPsLLAQK
S314-p IKSNFkPsLLAQKIE
K339-ub GVQVICMkGKAKYkA
Y344 CMkGKAKYkAsENAI
K345-ub MkGKAKYkAsENAIV
S347-p GKAKYkAsENAIVWk
K354-ac sENAIVWkIKRMAGM
K354-ub sENAIVWkIKRMAGM
K378-ub ELLPTNDkKKWARPP
K379 LLPTNDkKKWARPPI
K380 LPTNDkKKWARPPIS
K400 PFAPSGLKVRYLKVF
K405 GLKVRYLKVFEPkLN
K410-ub YLKVFEPkLNYSDHD
K420-ub YSDHDVIkWVRYIGR
Y431 YIGRSGIYETRC___
T433 GRSGIYETRC_____
3843 : Phospho-AP2M1 (Thr156) Antibody
7399 : Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb
  AP2M1 iso3  
S45 HARQQVRSPVTNIAR
S61 SFFHVKRSNIWLAAV
T134 GALKTFITQQGIKSQ
K139 FITQQGIKSQTKEEQ
K143 QGIKSQTKEEQSQIT
T150 KEEQSQITSQVtGQI
S151 EEQSQITSQVtGQIG
T154-p SQITSQVtGQIGWRR
K165 GWRREGIKYRRNELF
K201 VSGRVVMKSYLSGMP
Y203 GRVVMKSYLSGMPEC
K217 CKFGMNDKIVIEKQG
K222 NDKIVIEKQGKGTAD
K225 IVIEKQGKGTADETS
K233 GTADETSKSGKQSIA
S234 TADETSKSGKQSIAI
K236 DETSKSGKQSIAIDD
S238 TSKSGKQSIAIDDCT
K254 HQCVRLSKFDSERSI
K254 HQCVRLSKFDSERSI
S257 VRLSKFDSERSISFI
S260 SKFDSERSISFIPPD
K279 LMRYRTTKDIILPFR
K302 GRTKLEVKVVIKSNF
K306 LEVKVVIKSNFKPSL
K310 VVIKSNFKPSLLAQK
S312 IKSNFKPSLLAQKIE
K337 GVQVICMKGKAKYKA
Y342 CMKGKAKYKASENAI
K343 MKGKAKYKASENAIV
S345 GKAKYKASENAIVWK
K352 SENAIVWKIKRMAGM
K352 SENAIVWKIKRMAGM
K376 ELLPTNDKKKWARPP
K377 LLPTNDKKKWARPPI
K378 LPTNDKKKWARPPIS
K398 PFAPSGLKVRYLKVF
K403 GLKVRYLKVFEPKLN
K408 YLKVFEPKLNYSDHD
K418 YSDHDVIKWVRYIGR
Y429 YIGRSGIYETRC___
T431 GRSGIYETRC_____
  rat

 
S45 HARQQVRSPVTNIAR
S61 SFFHVKRSNIWLAAV
T134 GALKTFITQQGIKSQ
K139 FITQQGIKSQHQTKE
K145 IKSQHQTKEEQSQIT
T152 KEEQSQITSQVtGQI
S153 EEQSQITSQVtGQIG
T156-p SQITSQVtGQIGWRR
K167 GWRREGIKYRRNELF
K203 VSGRVVMKSyLSGMP
Y205-p GRVVMKSyLSGMPEC
K219 CKFGMNDKIVIEKQG
K224 NDKIVIEKQGKGTAD
K227 IVIEKQGKGTADETS
K235 GTADETSKSGKQSIA
S236 TADETSKSGKQSIAI
K238 DETSKSGKQSIAIDD
S240 TSKSGKQSIAIDDCT
K256 HQCVRLSKFDSERSI
K256 HQCVRLSKFDSERSI
S259 VRLSKFDSERSISFI
S262 SKFDSERSISFIPPD
K281 LMRYRTTKDIILPFR
K304 GRTKLEVKVVIKSNF
K308 LEVKVVIKSNFKPSL
K312 VVIKSNFKPSLLAQK
S314 IKSNFKPSLLAQKIE
K339 GVQVICMKGKAKYKA
Y344 CMKGKAKYKASENAI
K345 MKGKAKYKASENAIV
S347 GKAKYKASENAIVWK
K354 SENAIVWKIKRMAGM
K354 SENAIVWKIKRMAGM
K378 ELLPTNDKKKWARPP
K379 LLPTNDKKKWARPPI
K380 LPTNDKKKWARPPIS
K400-ac PFAPSGLkVRYLkVF
K405-ac GLkVRYLkVFEPKLN
K410 YLkVFEPKLNYSDHD
K420 YSDHDVIKWVRYIGR
Y431 YIGRSGIYETRC___
T433 GRSGIYETRC_____
3843 : Phospho-AP2M1 (Thr156) Antibody
7399 : Phospho-AP2M1 (Thr156) (D4F3) Rabbit mAb
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