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Protein Page:
TIF1G (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
TIF1G an atypical protein kinase that functions as a transcriptional repressor. Translocation with RET generates the TRIM33/RET (PTC7) oncogene. The protein is a member of the tripartite motif family. This motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Two splice variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin conjugating system; Protein kinase, atypical; Ubiquitin ligase; EC 6.3.2.-; Ligase; EC 6.3.2.19; Kinase, protein; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family
Cellular Component: nucleoplasm; intracellular membrane-bound organelle; nucleolus; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: transcription initiation from RNA polymerase II promoter; regulation of transforming growth factor beta receptor signaling pathway; transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; protein ubiquitination; gene expression; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of BMP signaling pathway
Reference #:  Q9UPN9 (UniProtKB)
Alt. Names/Synonyms: E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; FLJ32925; KIAA1113; Protein Rfg7; PTC7; RET-fused gene 7 protein; RFG7; TF1G; TIF1-gamma; TIF1G; TIF1GAMMA; TIFGAMMA; Transcription intermediary factor 1-gamma; transcriptional intermediary factor 1 gamma; TRI33; TRIM33; tripartite motif-containing 33; Tripartite motif-containing protein 33
Gene Symbols: TRIM33
Molecular weight: 122,533 Da
Basal Isoelectric point: 6.23  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TIF1G

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K252-ac HQRVKFTkDHLIRKK
0 2 K320-ub EEAFQNQkGAIENLL
0 1 K394-ub VTKERQMkLLQQQND
0 1 R440-m1 RLITFQLrHILKARC
0 1 R440 RLITFQLRHILKARC
0 1 T505-p GTNHISKtPGQINLA
0 9 R515-m1 QINLAQLrLQHMQQQ
0 4 R515-m2 QINLAQLrLQHMQQQ
0 16 Y524-p QHMQQQVyAQKHQQL
0 19 R535-m1 HQQLQQMrMQQPPAP
0 1 R577-m2 ISVQTMQrGNMNCGA
0 8 R591-m1 AFQAHQMrLAQNAAr
0 1 R591-m2 AFQAHQMrLAQNAAr
0 3 R598-m2 rLAQNAArIPGIPrH
0 4 R604-m2 ArIPGIPrHSGPQYS
0 1 S606 IPGIPrHSGPQYSMM
0 1 Y610 PrHSGPQYSMMQPHL
0 1 S611 rHSGPQYSMMQPHLQ
0 1 R619 MMQPHLQRQHSNPGH
0 1 S634 AGPFPVVSVHNTTIN
0 2 S644-p NTTINPTsPTTATMA
0 1 S661-p NRGPTSPsVTAIELI
0 21 K763-ac SSGRTAEkTSLSFkS
0 25 K769-ac EkTSLSFkSDQVKVk
0 5 K776-ac kSDQVKVkQEPGTED
0 1 K776-sm kSDQVKVkQEPGTED
0 10 K793-ac CSFSGGVkQEKTEDG
0 2 K793-sm CSFSGGVkQEKTEDG
0 18 S803-p KTEDGRRsACMLssP
0 13 S808-p RRsACMLssPEssLt
0 15 S809-p RsACMLssPEssLtP
0 10 S812-p CMLssPEssLtPPLs
0 7 S813-p MLssPEssLtPPLst
0 33 T815-p ssPEssLtPPLstNL
0 5 S819-p ssLtPPLstNLHLES
0 1 T820-p sLtPPLstNLHLESE
0 1 D829-ca LHLESELdALASLEN
0 2 S852-p MNESCKQsGLssLVN
0 2 S855-p SCKQsGLssLVNGks
0 2 S856-p CKQsGLssLVNGksP
0 1 K861-ac LssLVNGksPIRSLM
0 19 S862-p ssLVNGksPIRSLMH
0 3 K950-ac CDNLQHSkkGkTAQG
0 1 K951-ac DNLQHSkkGkTAQGL
0 4 K953-ac LQHSkkGkTAQGLsP
0 1 T954 QHSkkGkTAQGLsPV
0 2 S959-p GkTAQGLsPVDQRKC
0 1 S1004 IKKPMDLSTVKKKLQ
0 1 Y1048-p MMKVVQVyADtQEIN
0 1 T1051-p VVQVyADtQEINLKA
0 1 S1060 EINLKADSEVAQAGK
0 18 T1102-p EEDDGEVtEDsDEDF
0 18 S1105-p DGEVtEDsDEDFIQP
0 95 S1119-p PRRKRLKsDERPVHI
0 1 K1127-ac DERPVHIk_______
  TIF1G iso2  
K252 HQRVKFTKDHLIRKK
K320 EEAFQNQKGAIENLL
K394 VTKERQMKLLQQQND
R440 RLITFQLRHILKARC
R440 RLITFQLRHILKARC
T505 GTNHISKTPGQINLA
R515 QINLAQLRLQHMQQQ
R515 QINLAQLRLQHMQQQ
Y524 QHMQQQVYAQKHQQL
R535 HQQLQQMRMQQPPAP
R577 ISVQTMQRGNMNCGA
R591 AFQAHQMRLAQNAAR
R591 AFQAHQMRLAQNAAR
R598 RLAQNAARIPGIPRH
R604 ARIPGIPRHSGPQYS
S606 IPGIPRHSGPQYSMM
Y610 PRHSGPQYSMMQPHL
S611 RHSGPQYSMMQPHLQ
R619 MMQPHLQRQHSNPGH
S634 AGPFPVVSVHNTTIN
S644 NTTINPTSPTTATMA
S661 NRGPTSPSVTAIELI
K763 SSGRTAEKTSLSFKS
K769 EKTSLSFKSDQVKVK
K776 KSDQVKVKQEPGTED
K776 KSDQVKVKQEPGTED
K793 CSFSGGVKQEKTEDG
K793 CSFSGGVKQEKTEDG
S803 KTEDGRRSACMLSSP
S808 RRSACMLSSPESSLT
S809 RSACMLSSPESSLTP
S812 CMLSSPESSLTPPLS
S813 MLSSPESSLTPPLST
T815 SSPESSLTPPLSTNL
S819 SSLTPPLSTNLHLES
T820 SLTPPLSTNLHLESE
D829 LHLESELDALASLEN
S852 MNESCKQSGLSSLVN
S855 SCKQSGLSSLVNGKS
S856 CKQSGLSSLVNGKSP
K861 LSSLVNGKSPIRSLM
S862 SSLVNGKSPIRSLMH
K950 CDNLQHSKKGKTAQG
K951 DNLQHSKKGKTAQGL
K953 LQHSKKGKTAQGLSP
T954 QHSKKGKTAQGLSPV
S959 GKTAQGLSPVDQRKC
S1004 IKKPMDLSTVKKKLQ
- gap
- gap
S1043-p ERFNEADsEVAQAGK
T1085 EEDDGEVTEDSDEDF
S1088 DGEVTEDSDEDFIQP
S1102 PRRKRLKSDERPVHI
K1110 DERPVHIK_______
  mouse

 
K268 HQRVKFTKDHLIRKK
K336-ub EEAFQNQkGAIENLL
K410 VTKERQMKLLQQQND
R456 RLITFQLRHILKARC
R456-m2 RLITFQLrHILKARC
T521 GTNHISKTPGQINLA
R531-m1 QINLAQLrLQHMQQQ
R531-m2 QINLAQLrLQHMQQQ
Y540 QHMQQQVYAQKHQQL
R551-m1 HQQLQQMrLQQPPAP
R593-m2 ISVQTMQrGNMNCGA
R607-m1 AFQAHQMrLAQNAAr
R607-m2 AFQAHQMrLAQNAAr
R614-m2 rLAQNAArIPGIPrH
R620-m2 ArIPGIPrHsAPQys
S622-p IPGIPrHsAPQysMM
Y626-p PrHsAPQysMMQPHL
S627-p rHsAPQysMMQPHLQ
R635-m2 MMQPHLQrQHSNPGH
S650-gl AGPFPVVsAHNPINP
S659 HNPINPTSPTTATMA
S676 NRGPTSPSVTAIELI
K778-ac SSGRTAEkSAHSFKS
K784 EkSAHSFKSDQVKVK
K791 KSDQVKVKQEPGTEE
K791 KSDQVKVKQEPGTEE
K808 CSFSGAVKQEKTEDG
K808 CSFSGAVKQEKTEDG
S818-p KTEDGRRsACMLssP
S823-p RRsACMLssPEssLt
S824-p RsACMLssPEssLtP
S827-p CMLssPEssLtPPLs
S828-p MLssPEssLtPPLsT
T830-p ssPEssLtPPLsTNL
S834-p ssLtPPLsTNLHLES
T835 sLtPPLsTNLHLESE
D844 LHLESELDTLTGLEN
S867 ISESCKQSGLsNLVN
S870-p SCKQSGLsNLVNGKs
N871 CKQSGLsNLVNGKsP
K876 LsNLVNGKsPIRNLM
S877-p sNLVNGKsPIRNLMH
K965 CDNMQHSKKGktAQG
K966 DNMQHSKKGktAQGL
K968-ac MQHSKKGktAQGLsP
T969-p QHSKKGktAQGLsPV
S974-p GktAQGLsPVDQRKC
S1019-p IKKPMDLsTVKKKLQ
Y1063 MMKVVQVYADTQEIN
T1066 VVQVYADTQEINLKG
S1075 EINLKGDSEVAKAGK
T1117-p DEDDGEVtEDsDEDF
S1120-p DGEVtEDsDEDFIQP
S1134-p PRRKRLKsDERPVHI
K1142 DERPVHIK_______
  rat

 
K174 HQRVKFTKDHLIRKK
K242 EEAFQNQKGAIENLL
K316 VTKERQMKLLQQQND
R362 RLITFQLRHILKARC
R362 RLITFQLRHILKARC
T427 GTNHISKTPGQINLA
R437 QINLAQLRLQHMQQQ
R437 QINLAQLRLQHMQQQ
Y446 QHMQQQVYAQKHQQL
R457-m1 HQQLQQMrLQQPPAP
R499 ISVQTMQRGNMNCGA
R513-m1 AFQAHQMrLAQNAAR
R513 AFQAHQMRLAQNAAR
R520 rLAQNAARIPGIPRH
R526 ARIPGIPRHSGPQYS
S528 IPGIPRHSGPQYSMM
Y532 PRHSGPQYSMMQPHL
S533 RHSGPQYSMMQPHLQ
R541 MMQPHLQRQHSNPGH
S556 AGPFPVVSAHNPINP
S565 HNPINPTSPTTATMA
S582 NRGPTSPSVTAIELI
K684 SSGRTAEKSAHSFKS
K690 EKSAHSFKSDQVKVK
K697 KSDQVKVKQEPGTEE
K697 KSDQVKVKQEPGTEE
K714 CSFSGAVKQEKTEDG
K714 CSFSGAVKQEKTEDG
S724 KTEDGRRSACMLSSP
S729 RRSACMLSSPESSLT
S730 RSACMLSSPESSLTP
S733 CMLSSPESSLTPPLS
S734 MLSSPESSLTPPLST
T736 SSPESSLTPPLSTNL
S740 SSLTPPLSTNLHLES
T741 SLTPPLSTNLHLESE
D750 LHLESELDTLTSLEN
S773-p ISESCKQsGLSSLVN
S776 SCKQsGLSSLVNGKS
S777 CKQsGLSSLVNGKSP
K782 LSSLVNGKSPIRNLM
S783 SSLVNGKSPIRNLMH
K871 CDNMQHSKKGKTAQG
K872 DNMQHSKKGKTAQGL
K874 MQHSKKGKTAQGLSP
T875 QHSKKGKTAQGLSPV
S880 GKTAQGLSPVDQRKC
S925 IKKPMDLSTVKKKLQ
- gap
- gap
S964 ERFNEADSEVAQAGK
T1006-p EEDDGEVtEDsDEDF
S1009-p DGEVtEDsDEDFIQP
S1023 PRRKRLKSDERPVHI
K1031 DERPVHIK_______
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