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TIF1B (human)

Overview TIF1B an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Note: This description may include information from UniProtKB. Protein type: Protein kinase, atypical; Ligase; Kinase, protein; Ubiquitin conjugating system; EC 6.3.2.-; Nuclear receptor co-regulator; Ubiquitin ligase; Protein kinase, ATYPICAL; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription factor activity; transcription corepressor activity; protein kinase activity Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; epithelial to mesenchymal transition; gene expression; negative regulation of transcription, DNA-dependent Reference #:  Q13263 (UniProtKB) Alt. Names/Synonyms: FLJ29029; KAP-1; KAP1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; RNF96; TF1B; TIF1-beta; TIF1B; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; TRIM28; tripartite motif-containing 28; Tripartite motif-containing protein 28 Gene Symbols: TRIM28 Molecular weight: 88,550 Da Basal Isoelectric point: 5.52  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below TIF1B 1FP0 - A=619-679 (human) 2RO1 - A=624-812 (human) 2YVR - A/B=201-250 (human)

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1FP0 2RO1 2YVR  |  Phospho3D  |  DISEASE  |  Source  |  NURSA  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene

	Sites Implicated In
cell cycle regulation: S473‑p chromatin organization, altered: S824‑p DNA repair, induced: S824‑p transcription, altered: S824‑p sumoylation: S824‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		S4-p	____MAAsAAAAsAA
0	2		S9-p	AAsAAAAsAAAAsAA
0	1		S14-p	AAsAAAAsAAsGsPG
0	4		S17-p	AAAAsAAsGsPGPGE
1	19		S19-p	AAsAAsGsPGPGEGs
0	2		P22	AAsGsPGPGEGsAGG
0	5		S26-p	sPGPGEGsAGGEkRS
0	1		K31-a	EGsAGGEkRSTAPSA
0	3		S45-p	AAASASAsAAAssPA
0	7		S49-p	ASAsAAAssPAGGGA
1	19		S50-p	SAsAAAssPAGGGAE
0	2		A96	SACLGPAAPAAANSS
0	1		S103	APAAANSSGDGGAAG
0	2		K127-u	CKQQCFSkDIVENyF
0	1		Y133-p	SkDIVENyFMRDSGS
0	4		K213-u	TVYCNVHkHEPLVLF
0	28		K238-u	DCQLNAHkDHQyQFL
0	4		Y242-p	NAHkDHQyQFLEDAV
0	22		K254-u	DAVRNQRkLLAsLVk
1	2		S258-p	NQRkLLAsLVkRLGD
0	12		K261-u	kLLAsLVkRLGDkHA
0	1		K266-a	LVkRLGDkHATLQkS
0	6		K266-u	LVkRLGDkHATLQkS
0	22		K272-u	DkHATLQkSTkEVRS
0	1		K275-u	ATLQkSTkEVRSSIR
0	3		K289-u	RQVSDVQkRVQVDVk
0	1		K296-u	kRVQVDVkMAILQIM
0	1		K304-a	MAILQIMkELNkRGR
0	5		K304-u	MAILQIMkELNkRGR
0	1		K308-u	QIMkELNkRGRVLVN
0	108		K319-u	VLVNDAQkVTEGQQE
0	1		K337-u	RQHWTMTkIQkHQEH
0	1		K340-a	WTMTkIQkHQEHILR
0	22		K340-u	WTMTkIQkHQEHILR
0	4		K365-u	NTALLLSkkLIyFQL
0	14		K366-u	TALLLSkkLIyFQLH
0	38		Y369-p	LLSkkLIyFQLHRAL
0	1		K377-a	FQLHRALkMIVDPVE
0	9		K377-u	FQLHRALkMIVDPVE
0	1		K390-u	VEPHGEMkFQWDLNA
0	58		K400-u	WDLNAWTkSAEAFGk
0	33		K407-u	kSAEAFGkIVAERPG
0	6		K434-u	RAPGPLSkQGsGssQ
1	5		S437-p	GPLSkQGsGssQPME
0	1		S439-p	LSkQGsGssQPMEVQ
2	4		S440-p	SkQGsGssQPMEVQE
0	2		Y449-p	PMEVQEGyGFGsGDD
0	1		S453-p	QEGyGFGsGDDPyss
0	108		Y458-p	FGsGDDPyssAEPHV
0	6		S459-p	GsGDDPyssAEPHVs
0	11		S460-p	sGDDPyssAEPHVsG
0	2		S466-p	ssAEPHVsGVkRsRs
0	5		K469-a	EPHVsGVkRsRsGEG
0	8		K469-u	EPHVsGVkRsRsGEG
0	12		S471-p	HVsGVkRsRsGEGEV
5	69		S473-p	sGVkRsRsGEGEVsG
0	2		S479-p	RsGEGEVsGLMRKVP
1	10		S489-p	MRKVPRVsLERLDLD
0	2		T498-p	ERLDLDLtADsQPPV
0	1		A499	RLDLDLtADsQPPVF
1	18		S501-p	DLDLtADsQPPVFkV
0	5		K507-u	DsQPPVFkVFPGSTt
0	5		T514-p	kVFPGSTtEDyNLIV
0	135		Y517-p	PGSTtEDyNLIVIER
0	1		T536-p	AATGQPGtAPAGtPG
0	8		T541-p	PGtAPAGtPGAPPLA
4	0		K554-s	LAGMAIVkEEETEAA
1	0		K575	ATEGPETKPVLMALA
0	1		K575-u	ATEGPETkPVLMALA
1	14		S594-p	AEGPRLAsPsGstss
1	4		S596-p	GPRLAsPsGstssGL
0	5		S598-p	RLAsPsGstssGLEV
0	5		T599-p	LAsPsGstssGLEVV
0	5		S600-p	AsPsGstssGLEVVA
0	9		S601-p	sPsGstssGLEVVAP
0	1		T611-p	EVVAPEGtsAPGGGP
0	1		S612-p	VVAPEGtsAPGGGPG
0	1		G616	EGtsAPGGGPGTLDD
0	2		S624-p	GPGTLDDsATICRVC
1	0		K676	CHVLPDLKEEDGsLs
0	1		S681-p	DLKEEDGsLsLdGAD
0	3		S683-p	KEEDGsLsLdGADST
0	1		D685-ca	EDGsLsLdGADSTGV
0	1		T690	sLdGADSTGVVAKLs
0	6		S697-p	TGVVAKLsPANQRKC
0	65		K750-u	IRARLQEkLsPPYss
2	1		K750-s	IRARLQEkLsPPYss
1	20		S752-p	ARLQEkLsPPYssPQ
0	1		Y755	QEkLsPPYssPQEFA
1	9		S756-p	EkLsPPYssPQEFAQ
1	27		S757-p	kLsPPYssPQEFAQD
0	18		K770-a	QDVGRMFkQFNkLTE
0	7		K770-u	QDVGRMFkQFNkLTE
0	1		K774-a	RMFkQFNkLTEDkAD
0	15		K774-u	RMFkQFNkLTEDkAD
0	44		K779-u	FNkLTEDkADVQsII
0	1		K779-a	FNkLTEDkADVQsII
5	2		K779-s	FNkLTEDkADVQsII
0	2		S784-p	EDkADVQsIIGLQRF
4	1		K804-s	NEAFGDTkFSAVLVE
0	1		K804-u	NEAFGDTkFSAVLVE
0	2		S816-p	LVEPPPMsLPGAGLs
2	4		S823-p	sLPGAGLssQELsGG
14	12		S824-p	LPGAGLssQELsGGP
0	1		S828-p	GLssQELsGGPGDGP

	mouse
S4	____MAASAAATAAA
S12	AAATAAASAATAASA
S18	ASAATAASAAsGsPG
S21-p	ATAASAAsGsPGsGE
S23-p	AASAAsGsPGsGEGs
S26-p	AAsGsPGsGEGsAGG
S30-p	sPGsGEGsAGGEKRP
K35	EGsAGGEKRPAASSA
S46-p	ASSAAAAsAAAssPA
S50-p	AAAsAAAssPAGGGG
S51-p	AAsAAAssPAGGGGE
T97-p	SACLGPAtPAAANNs
S104-p	tPAAANNsGDGGSAG
K128-u	CKQQCYSkDIVENYF
Y134	SkDIVENYFMRDSGS
K214	TVYCNVHKHEPLVLF
K239-u	DCQLNAHkDHQYQFL
Y243	NAHkDHQYQFLEDAV
K255-u	DAVRNQRkLLASLVk
S259	NQRkLLASLVkRLGD
K262-u	kLLASLVkRLGDKHA
K267	LVkRLGDKHATLQkN
K267	LVkRLGDKHATLQkN
K273-u	DKHATLQkNTKEVRS
K276	ATLQkNTKEVRSSIR
K290	RQVSDVQKRVQVDVK
K297	KRVQVDVKMAILQIM
K305	MAILQIMKELNKRGR
K305-u	MAILQIMkELNKRGR
K309	QIMkELNKRGRVLVN
K320-u	VLVNDAQkVTEGQQE
K338	RQHWTMTKIQKHQEH
K341	WTMTKIQKHQEHILR
K341	WTMTKIQKHQEHILR
K366	NTALLLSKKLIyFQL
K367	TALLLSKKLIyFQLH
Y370-p	LLSKKLIyFQLHRAL
K378	FQLHRALKMIVDPVE
K378-u	FQLHRALkMIVDPVE
K391	VEPHGEMKFQWDLNA
K401-u	WDLNAWTkSAEAFGk
K408-u	kSAEAFGkIVAERPG
K435-u	RAPGPLSkQGsGSSQ
S438-p	GPLSkQGsGSSQPME
S440	LSkQGsGSSQPMEVQ
S441	SkQGsGSSQPMEVQE
Y450	PMEVQEGYGFGSDDP
S454	QEGYGFGSDDPySSA
Y458-p	GFGSDDPySSAEPHV
S459	FGSDDPySSAEPHVS
S460	GSDDPySSAEPHVSG
S466	SSAEPHVSGMkRsRs
K469-a	EPHVSGMkRsRsGEG
K469-u	EPHVSGMkRsRsGEG
S471-p	HVSGMkRsRsGEGEV
S473-p	SGMkRsRsGEGEVSG
S479	RsGEGEVSGLLRKVP
S489-p	LRKVPRVsLERLDLD
T498	ERLDLDLTsDsQPPV
S499-p	RLDLDLTsDsQPPVF
S501-p	DLDLTsDsQPPVFkV
K507-u	DsQPPVFkVFPGSTT
T514	kVFPGSTTEDYNLIV
Y517	PGSTTEDYNLIVIER
T536	AAAGQAGTVPPGAPG
A541	AGTVPPGAPGAPPLP
K554-s	LPGMAIVkEEETEAA
K575-s	APEGPETkPVLMPLT
K575	APEGPETKPVLMPLT
S594-p	AEGPRLAsPsGstss
S596-p	GPRLAsPsGstssGL
S598-p	RLAsPsGstssGLEV
T599-p	LAsPsGstssGLEVV
S600-p	AsPsGstssGLEVVA
S601-p	sPsGstssGLEVVAP
T611	EVVAPEVTsAPVsGP
S612-p	VVAPEVTsAPVsGPG
S616-p	EVTsAPVsGPGILDD
S624-p	GPGILDDsATICRVC
K676-s	CHVLPDLkEEDGSLS
S681	DLkEEDGSLSLDGAD
S683	kEEDGSLSLDGADST
D685	EDGSLSLDGADSTGV
T690	SLDGADSTGVVAKLs
S697-p	TGVVAKLsPANQRKC
K750-u	IRARLQEkLsPPyss
K750-s	IRARLQEkLsPPyss
S752-p	ARLQEkLsPPyssPQ
Y755-p	QEkLsPPyssPQEFA
S756-p	EkLsPPyssPQEFAQ
S757-p	kLsPPyssPQEFAQD
K770	QDVGRMFKQFNkLTE
K770-u	QDVGRMFkQFNkLTE
K774	RMFkQFNKLTEDkAD
K774-u	RMFkQFNkLTEDkAD
K779-u	FNkLTEDkADVQsII
K779	FNkLTEDKADVQsII
K779-s	FNkLTEDkADVQsII
S784-p	EDkADVQsIIGLQRF
K804-s	NDAFGDTkFSAVLVE
K804	NDAFGDTKFSAVLVE
N816	LVEPPPLNLPSAGLs
S823-p	NLPSAGLssQELSGP
S824-p	LPSAGLssQELSGPG
S828	GLssQELSGPGDGP_

	rat
S4	____MAASAAAATAA
S13	AAATAAASAATAASA
S19	ASAATAASAASGSPG
S22	ATAASAASGSPGSGE
S24	AASAASGSPGSGEGS
S27	AASGSPGSGEGSAGG
S31	SPGSGEGSAGGEKRP
K36	EGSAGGEKRPAASSA
S47	ASSAAAASASAssPA
S51-p	AAASASAssPAGGGG
S52-p	AASASAssPAGGGGE
T98	SACLGPATPAAANNS
S105	TPAAANNSGDGGSAG
K129	CKQQCYSKDIVENYF
Y135	SKDIVENYFMRDSGS
K215	TVYCNVHKHEPLVLF
K240	DCQLNAHKDHQYQFL
Y244	NAHKDHQYQFLEDAV
K256	DAVRNQRKLLASLVK
S260	NQRKLLASLVKRLGD
K263	KLLASLVKRLGDKHA
K268	LVKRLGDKHATLQKN
K268	LVKRLGDKHATLQKN
K274	DKHATLQKNTKEVRS
K277	ATLQKNTKEVRSSIR
K291	RQVSDVQKRVQVDVK
K298	KRVQVDVKMAILQIM
K306	MAILQIMKELNKRGR
K306	MAILQIMKELNKRGR
K310	QIMKELNKRGRVLVN
K321	VLVNDAQKVTEGQQE
K339	RQHWTMTKIQKHQEH
K342	WTMTKIQKHQEHILR
K342	WTMTKIQKHQEHILR
K367	NTALLLSKKLIYFQL
K368	TALLLSKKLIYFQLH
Y371	LLSKKLIYFQLHRAL
K379	FQLHRALKMIVDPVE
K379	FQLHRALKMIVDPVE
K392	VEPHGEMKFQWDLNA
K402	WDLNAWTKSAEAFGK
K409	KSAEAFGKIVAERPG
K436	RAPGPLSKQGSGSSQ
S439	GPLSKQGSGSSQPME
S441	LSKQGSGSSQPMEVQ
S442	SKQGSGSSQPMEVQE
Y451	PMEVQEGYGFGTDDP
T455	QEGYGFGTDDPYSSA
Y459	GFGTDDPYSSAEPHV
S460	FGTDDPYSSAEPHVS
S461	GTDDPYSSAEPHVSG
S467	SSAEPHVSGMKRsRs
K470	EPHVSGMKRsRsGEG
K470	EPHVSGMKRsRsGEG
S472-p	HVSGMKRsRsGEGEV
S474-p	SGMKRsRsGEGEVSG
S480	RsGEGEVSGLMRKVP
S490	MRKVPRVSLERLDLD
T499	ERLDLDLTSDSQPPV
S500	RLDLDLTSDSQPPVF
S502	DLDLTSDSQPPVFKV
K508	DSQPPVFKVFPGSTT
T515	KVFPGSTTEDYNLIV
Y518	PGSTTEDYNLIVIER
T537	AAAGQAGTVPPGAPG
A542	AGTVPPGAPGAPPLP
K555	LPGMAIVKEEETEAA
K576	APEGPETKPVLMALT
K576	APEGPETKPVLMALT
S595-p	AEGPRLAsPSGSTSs
S597	GPRLAsPSGSTSsGL
S599	RLAsPSGSTSsGLEV
T600	LAsPSGSTSsGLEVV
S601	AsPSGSTSsGLEVVA
S602-p	sPSGSTSsGLEVVAP
T612	EVVAPEVTSAPVSGP
S613	VVAPEVTSAPVSGPG
S617	EVTSAPVSGPGILDD
S625	GPGILDDSATICRVC
K677	CHVLPDLKEEDGSLS
S682	DLKEEDGSLSLDGAD
S684	KEEDGSLSLDGADSt
D686	EDGSLSLDGADStGV
T691-p	SLDGADStGVVAKLs
S698-p	tGVVAKLsPANQRKC
K751	IRARLQEKLSPPYSS
K751	IRARLQEKLSPPYSS
S753	ARLQEKLSPPYSSPQ
Y756	QEKLSPPYSSPQEFA
S757	EKLSPPYSSPQEFAQ
S758	KLSPPYSSPQEFAQD
K771	QDVGRMFKQFNKLTE
K771	QDVGRMFKQFNKLTE
K775	RMFKQFNKLTEDKAD
K775	RMFKQFNKLTEDKAD
K780	FNKLTEDKADVQSII
K780	FNKLTEDKADVQSII
K780	FNKLTEDKADVQSII
S785	EDKADVQSIIGLQRF
K805	NDAFGDTKFSAVLVE
K805	NDAFGDTKFSAVLVE
N817	LVEPPPLNLPSAGLS
S824	NLPSAGLSSQELSGP
S825	LPSAGLSSQELSGPG
S829	GLSSQELSGPGDGP_

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