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Protein Page:
TIF1B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
TIF1B an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, atypical; Nuclear receptor co-regulator; Ubiquitin ligase; Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Kinase, protein; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family
Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus
Molecular Function: protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription factor activity; transcription corepressor activity; ligase activity; protein kinase activity
Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; positive regulation of transcription, DNA-dependent; protein amino acid autophosphorylation; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; innate immune response; epithelial to mesenchymal transition; gene expression; negative regulation of transcription, DNA-dependent
Reference #:  Q13263 (UniProtKB)
Alt. Names/Synonyms: FLJ29029; KAP-1; KAP1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; RNF96; TF1B; TIF1-beta; TIF1B; Transcription intermediary factor 1-beta; transcriptional intermediary factor 1-beta; TRIM28; tripartite motif-containing 28; Tripartite motif-containing protein 28
Gene Symbols: TRIM28
Molecular weight: 88,550 Da
Basal Isoelectric point: 5.52  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TIF1B

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: S473‑p
chromatin organization, altered: S824‑p
DNA repair, induced: Y449‑p, Y458‑p, S473‑p, Y517‑p, S824‑p
transcription, induced: S824‑p
molecular association, regulation: Y449‑p, Y458‑p, Y517‑p
phosphorylation: Y449‑p, Y458‑p, Y517‑p
sumoylation: S824‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S4-p ____MAAsAAAAsAA
0 3 S9-p AAsAAAAsAAAAsAA
0 1 S14-p AAsAAAAsAAsGsPG
0 5 S17-p AAAAsAAsGsPGPGE
1 24 S19-p AAsAAsGsPGPGEGs
0 3 P22 AAsGsPGPGEGsAGG
0 7 S26-p sPGPGEGsAGGEkRS
0 2 K31-ac EGsAGGEkRStAPsA
0 1 T34-p AGGEkRStAPsAAAS
0 1 S37-p EkRStAPsAAASASA
0 5 S45-p AAASASAsAAAssPA
0 10 S49-p ASAsAAAssPAGGGA
1 25 S50-p SAsAAAssPAGGGAE
0 2 A96 SACLGPAAPAAANSS
0 1 S103 APAAANSSGDGGAAG
0 2 K127-ub CKQQCFSkDIVENyF
0 1 Y133-p SkDIVENyFMRDsGS
0 2 S138-p ENyFMRDsGSKAATD
0 4 K213-ub TVYCNVHkHEPLVLF
0 28 K238-ub DCQLNAHkDHQyQFL
0 4 Y242-p NAHkDHQyQFLEDAV
0 22 K254-ub DAVRNQRkLLAsLVk
1 5 S258-p NQRkLLAsLVkRLGD
0 12 K261-ub kLLAsLVkRLGDkHA
0 1 K266-ac LVkRLGDkHATLQkS
0 6 K266-ub LVkRLGDkHATLQkS
0 22 K272-ub DkHATLQkSTkEVRS
0 1 K275-ub ATLQkSTkEVRSSIR
0 3 K289-ub RQVSDVQkRVQVDVk
0 1 K296-ub kRVQVDVkMAILQIM
0 1 K304-ac MAILQIMkELNkRGR
0 5 K304-ub MAILQIMkELNkRGR
0 1 K308-ub QIMkELNkRGRVLVN
0 108 K319-ub VLVNDAQkVTEGQQE
0 1 K337-ub RQHWTMTkIQkHQEH
0 1 K340-ac WTMTkIQkHQEHILR
0 22 K340-ub WTMTkIQkHQEHILR
0 1 S355-p FASWALEsDNNTALL
0 4 K365-ub NTALLLSkkLIyFQL
0 14 K366-ub TALLLSkkLIyFQLH
0 40 Y369-p LLSkkLIyFQLHRAL
0 2 K377-ac FQLHRALkMIVDPVE
0 9 K377-ub FQLHRALkMIVDPVE
0 1 K390-ub VEPHGEMkFQWDLNA
0 58 K400-ub WDLNAWTkSAEAFGk
0 33 K407-ub kSAEAFGkIVAERPG
0 2 S417-p AERPGTNsTGPAPMA
0 6 K434-ub RAPGPLSkQGsGssQ
1 6 S437-p GPLSkQGsGssQPME
0 2 S439-p LSkQGsGssQPMEVQ
2 5 S440-p SkQGsGssQPMEVQE
1 3 Y449-p PMEVQEGyGFGsGDD
0 2 S453-p QEGyGFGsGDDPyss
1 104 Y458-p FGsGDDPyssAEPHV
0 7 S459-p GsGDDPyssAEPHVs
0 12 S460-p sGDDPyssAEPHVsG
0 3 S466-p ssAEPHVsGVkRsRs
0 5 K469-ac EPHVsGVkRsRsGEG
0 8 K469-ub EPHVsGVkRsRsGEG
0 16 S471-p HVsGVkRsRsGEGEV
9 83 S473-p sGVkRsRsGEGEVsG
0 4 S479-p RsGEGEVsGLMRKVP
1 18 S489-p MRKVPRVsLERLDLD
0 4 T498-p ERLDLDLtADsQPPV
0 1 A499 RLDLDLtADsQPPVF
1 23 S501-p DLDLtADsQPPVFkV
0 5 K507-ub DsQPPVFkVFPGSTt
0 5 T514-p kVFPGSTtEDyNLIV
1 129 Y517-p PGSTtEDyNLIVIER
0 1 T531-p RGAAAAAtGQPGtAP
0 3 T536-p AAtGQPGtAPAGtPG
0 11 T541-p PGtAPAGtPGAPPLA
4 0 K554-sm LAGMAIVkEEETEAA
0 1 K575-ac ATEGPETkPVLMALA
1 0 K575 ATEGPETKPVLMALA
0 1 K575-ub ATEGPETkPVLMALA
1 17 S594-p AEGPRLAsPsGstss
1 6 S596-p GPRLAsPsGstssGL
0 7 S598-p RLAsPsGstssGLEV
0 7 T599-p LAsPsGstssGLEVV
0 10 S600-p AsPsGstssGLEVVA
0 14 S601-p sPsGstssGLEVVAP
0 1 T611-p EVVAPEGtsAPGGGP
0 1 S612-p VVAPEGtsAPGGGPG
0 1 G616 EGtsAPGGGPGtLDD
0 2 T620-p APGGGPGtLDDsATI
0 5 S624-p GPGtLDDsATICRVC
1 0 K676 CHVLPDLKEEDGsLs
0 1 S681-p DLKEEDGsLsLdGAD
0 5 S683-p KEEDGsLsLdGADsT
0 1 D685-ca EDGsLsLdGADsTGV
0 1 S689-p LsLdGADsTGVVAKL
0 1 T690 sLdGADsTGVVAKLs
0 8 S697-p TGVVAKLsPANQRKC
0 65 K750-ub IRARLQEkLsPPyss
2 2 K750-sm IRARLQEkLsPPyss
1 22 S752-p ARLQEkLsPPyssPQ
0 2 Y755-p QEkLsPPyssPQEFA
1 9 S756-p EkLsPPyssPQEFAQ
1 30 S757-p kLsPPyssPQEFAQD
0 18 K770-ac QDVGRMFkQFNkLTE
0 7 K770-ub QDVGRMFkQFNkLTE
0 1 K774-ac RMFkQFNkLTEDkAD
0 15 K774-ub RMFkQFNkLTEDkAD
0 44 K779-ub FNkLTEDkADVQsII
0 2 K779-ac FNkLTEDkADVQsII
6 3 K779-sm FNkLTEDkADVQsII
0 3 S784-p EDkADVQsIIGLQRF
4 1 K804-sm NEAFGDTkFSAVLVE
0 1 K804-ub NEAFGDTkFSAVLVE
0 2 S816-p LVEPPPMsLPGAGLs
2 4 S823-p sLPGAGLssQELsGG
18 13 S824-p LPGAGLssQELsGGP
0 1 S828-p GLssQELsGGPGDGP
4127 : Phospho-TIF1beta (Ser824) Antibody
  mouse

 
S4 ____MAASAAATAAA
S12 AAATAAASAATAASA
S18 ASAATAASAAsGsPG
S21-p ATAASAAsGsPGsGE
S23-p AASAAsGsPGsGEGs
S26-p AAsGsPGsGEGsAGG
S30-p sPGsGEGsAGGEKRP
K35 EGsAGGEKRPAASSA
- gap
- gap
S46-p ASSAAAAsAAAssPA
S50-p AAAsAAAssPAGGGG
S51-p AAsAAAssPAGGGGE
T97-p SACLGPAtPAAANNs
S104-p tPAAANNsGDGGSAG
K128-ub CKQQCYSkDIVENYF
Y134 SkDIVENYFMRDSGS
S139 ENYFMRDSGSKASSD
K214 TVYCNVHKHEPLVLF
K239-ub DCQLNAHkDHQYQFL
Y243 NAHkDHQYQFLEDAV
K255-ub DAVRNQRkLLAsLVk
S259-p NQRkLLAsLVkRLGD
K262-ub kLLAsLVkRLGDKHA
K267 LVkRLGDKHATLQkN
K267 LVkRLGDKHATLQkN
K273-ub DKHATLQkNTKEVRS
K276 ATLQkNTKEVRSSIR
K290 RQVSDVQKRVQVDVK
K297 KRVQVDVKMAILQIM
K305 MAILQIMKELNKRGR
K305-ub MAILQIMkELNKRGR
K309 QIMkELNKRGRVLVN
K320-ub VLVNDAQkVTEGQQE
K338 RQHWTMTKIQKHQEH
K341 WTMTKIQKHQEHILR
K341 WTMTKIQKHQEHILR
S356 FASWALESDNNTALL
K366 NTALLLSKKLIyFQL
K367 TALLLSKKLIyFQLH
Y370-p LLSKKLIyFQLHRAL
K378 FQLHRALKMIVDPVE
K378-ub FQLHRALkMIVDPVE
K391 VEPHGEMKFQWDLNA
K401-ub WDLNAWTkSAEAFGk
K408-ub kSAEAFGkIVAERPG
S418 AERPGTNSTGPGPMA
K435-ub RAPGPLSkQGsGSSQ
S438-p GPLSkQGsGSSQPME
S440 LSkQGsGSSQPMEVQ
S441 SkQGsGSSQPMEVQE
Y450 PMEVQEGYGFGSDDP
S454 QEGYGFGSDDPySSA
Y458-p GFGSDDPySSAEPHV
S459 FGSDDPySSAEPHVS
S460 GSDDPySSAEPHVSG
S466 SSAEPHVSGMkRsRs
K469-ac EPHVSGMkRsRsGEG
K469-ub EPHVSGMkRsRsGEG
S471-p HVSGMkRsRsGEGEV
S473-p SGMkRsRsGEGEVSG
S479 RsGEGEVSGLLRKVP
S489-p LRKVPRVsLERLDLD
T498 ERLDLDLTsDsQPPV
S499-p RLDLDLTsDsQPPVF
S501-p DLDLTsDsQPPVFkV
K507-ub DsQPPVFkVFPGSTT
T514 kVFPGSTTEDYNLIV
Y517 PGSTTEDYNLIVIER
A531 RGAAAAAAGQAGTVP
T536 AAAGQAGTVPPGAPG
A541 AGTVPPGAPGAPPLP
K554-sm LPGMAIVkEEETEAA
K575 APEGPETKPVLMPLT
K575-sm APEGPETkPVLMPLT
K575 APEGPETKPVLMPLT
S594-p AEGPRLAsPsGstss
S596-p GPRLAsPsGstssGL
S598-p RLAsPsGstssGLEV
T599-p LAsPsGstssGLEVV
S600-p AsPsGstssGLEVVA
S601-p sPsGstssGLEVVAP
T611 EVVAPEVTsAPVsGP
S612-p VVAPEVTsAPVsGPG
S616-p EVTsAPVsGPGILDD
I620 APVsGPGILDDsATI
S624-p GPGILDDsATICRVC
K676-sm CHVLPDLkEEDGSLS
S681 DLkEEDGSLSLDGAD
S683 kEEDGSLSLDGADST
D685 EDGSLSLDGADSTGV
S689 LSLDGADSTGVVAKL
T690 SLDGADSTGVVAKLs
S697-p TGVVAKLsPANQRKC
K750-ub IRARLQEkLsPPyss
K750-sm IRARLQEkLsPPyss
S752-p ARLQEkLsPPyssPQ
Y755-p QEkLsPPyssPQEFA
S756-p EkLsPPyssPQEFAQ
S757-p kLsPPyssPQEFAQD
K770 QDVGRMFKQFNkLTE
K770-ub QDVGRMFkQFNkLTE
K774 RMFkQFNKLTEDkAD
K774-ub RMFkQFNkLTEDkAD
K779-ub FNkLTEDkADVQsII
K779 FNkLTEDKADVQsII
K779-sm FNkLTEDkADVQsII
S784-p EDkADVQsIIGLQRF
K804-sm NDAFGDTkFSAVLVE
K804 NDAFGDTKFSAVLVE
N816 LVEPPPLNLPSAGLs
S823-p NLPSAGLssQELSGP
S824-p LPSAGLssQELSGPG
S828 GLssQELSGPGDGP_
4127 : Phospho-TIF1beta (Ser824) Antibody
  rat

 
S4 ____MAASAAAATAA
S13 AAATAAASAATAASA
S19 ASAATAASAASGSPG
S22 ATAASAASGSPGSGE
S24 AASAASGSPGSGEGS
S27 AASGSPGSGEGSAGG
S31 SPGSGEGSAGGEKRP
K36 EGSAGGEKRPAASSA
- gap
- gap
S47 ASSAAAASASAssPA
S51-p AAASASAssPAGGGG
S52-p AASASAssPAGGGGE
T98 SACLGPATPAAANNS
S105 TPAAANNSGDGGSAG
K129 CKQQCYSKDIVENYF
Y135 SKDIVENYFMRDSGS
S140 ENYFMRDSGSKASSD
K215 TVYCNVHKHEPLVLF
K240 DCQLNAHKDHQYQFL
Y244 NAHKDHQYQFLEDAV
K256 DAVRNQRKLLASLVK
S260 NQRKLLASLVKRLGD
K263 KLLASLVKRLGDKHA
K268 LVKRLGDKHATLQKN
K268 LVKRLGDKHATLQKN
K274 DKHATLQKNTKEVRS
K277 ATLQKNTKEVRSSIR
K291 RQVSDVQKRVQVDVK
K298 KRVQVDVKMAILQIM
K306 MAILQIMKELNKRGR
K306 MAILQIMKELNKRGR
K310 QIMKELNKRGRVLVN
K321 VLVNDAQKVTEGQQE
K339 RQHWTMTKIQKHQEH
K342 WTMTKIQKHQEHILR
K342 WTMTKIQKHQEHILR
S357 FASWALESDNNTALL
K367 NTALLLSKKLIYFQL
K368 TALLLSKKLIYFQLH
Y371 LLSKKLIYFQLHRAL
K379 FQLHRALKMIVDPVE
K379 FQLHRALKMIVDPVE
K392 VEPHGEMKFQWDLNA
K402 WDLNAWTKSAEAFGK
K409 KSAEAFGKIVAERPG
S419 AERPGTNSTGPGPMA
K436 RAPGPLSKQGSGSSQ
S439 GPLSKQGSGSSQPME
S441 LSKQGSGSSQPMEVQ
S442 SKQGSGSSQPMEVQE
Y451 PMEVQEGYGFGTDDP
T455 QEGYGFGTDDPYSSA
Y459 GFGTDDPYSSAEPHV
S460 FGTDDPYSSAEPHVS
S461 GTDDPYSSAEPHVSG
S467 SSAEPHVSGMKRsRs
K470 EPHVSGMKRsRsGEG
K470 EPHVSGMKRsRsGEG
S472-p HVSGMKRsRsGEGEV
S474-p SGMKRsRsGEGEVSG
S480 RsGEGEVSGLMRKVP
S490 MRKVPRVSLERLDLD
T499 ERLDLDLTSDSQPPV
S500 RLDLDLTSDSQPPVF
S502 DLDLTSDSQPPVFKV
K508 DSQPPVFKVFPGSTT
T515 KVFPGSTTEDYNLIV
Y518 PGSTTEDYNLIVIER
A532 RGAAAAAAGQAGTVP
T537 AAAGQAGTVPPGAPG
A542 AGTVPPGAPGAPPLP
K555 LPGMAIVKEEETEAA
K576 APEGPETKPVLMALT
K576 APEGPETKPVLMALT
K576 APEGPETKPVLMALT
S595-p AEGPRLAsPSGSTSs
S597 GPRLAsPSGSTSsGL
S599 RLAsPSGSTSsGLEV
T600 LAsPSGSTSsGLEVV
S601 AsPSGSTSsGLEVVA
S602-p sPSGSTSsGLEVVAP
T612 EVVAPEVTSAPVSGP
S613 VVAPEVTSAPVSGPG
S617 EVTSAPVSGPGILDD
I621 APVSGPGILDDSATI
S625 GPGILDDSATICRVC
K677 CHVLPDLKEEDGSLS
S682 DLKEEDGSLSLDGAD
S684 KEEDGSLSLDGADSt
D686 EDGSLSLDGADStGV
S690 LSLDGADStGVVAKL
T691-p SLDGADStGVVAKLs
S698-p tGVVAKLsPANQRKC
K751 IRARLQEKLSPPYSS
K751 IRARLQEKLSPPYSS
S753 ARLQEKLSPPYSSPQ
Y756 QEKLSPPYSSPQEFA
S757 EKLSPPYSSPQEFAQ
S758 KLSPPYSSPQEFAQD
K771 QDVGRMFKQFNKLTE
K771 QDVGRMFKQFNKLTE
K775 RMFKQFNKLTEDKAD
K775 RMFKQFNKLTEDKAD
K780 FNKLTEDKADVQSII
K780 FNKLTEDKADVQSII
K780 FNKLTEDKADVQSII
S785 EDKADVQSIIGLQRF
K805 NDAFGDTKFSAVLVE
K805 NDAFGDTKFSAVLVE
N817 LVEPPPLNLPSAGLS
S824 NLPSAGLSSQELSGP
S825 LPSAGLSSQELSGPG
S829 GLSSQELSGPGDGP_
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