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Protein Page:
SMG1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
SMG1 Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ. Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2. Interacts with UPF2. Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver. Inhibited by caffeine, LY294002 and wortmannin. Belongs to the PI3/PI4-kinase family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, ATYPICAL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; ATYPICAL group; PIKK family; SMG1 subfamily
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding; protein kinase activity
Biological Process: peptidyl-serine phosphorylation; mRNA export from nucleus; phosphoinositide phosphorylation; RNA metabolic process; protein amino acid autophosphorylation; mRNA catabolic process, nonsense-mediated decay; response to stress; gene expression; DNA repair; mRNA metabolic process
Reference #:  Q96Q15 (UniProtKB)
Alt. Names/Synonyms: 61E3.4; ATX; hSMG-1; KIAA0421; Lambda-interacting protein; Lambda/iota protein kinase C-interacting protein; LIP; phosphatidylinositol 3-kinase-related kinase; phosphatidylinositol 3-kinase-related protein kinase; PI-3-kinase-related kinase SMG-1; Serine/threonine-protein kinase SMG1; SMG-1; SMG1; SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Gene Symbols: SMG1
Molecular weight: 410,501 Da
Basal Isoelectric point: 6.03  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SMG1
Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

 
0 1 S11 RAPGSRLSSGGGGGG
0 1 S12 APGSRLSSGGGGGGt
0 1 T19-p SGGGGGGtKYPRSWN
0 2 T32-p WNDWQPRtDSASADP
0 1 S34 DWQPRtDSASADPDN
0 1 Y44-p ADPDNLKySSSRDRG
0 1 S140-p MRKSQERsMsYSDEs
0 1 S142-p KSQERsMsYSDEsRL
0 5 S147-p sMsYSDEsRLSNLLR
0 19 K173-a LATVKQLkEFIQQPE
0 1 Y252-p KDEVKLLyLCAtyKA
0 1 T256-p KLLyLCAtyKALEtV
0 2 Y257-p LLyLCAtyKALEtVG
0 1 T262-p AtyKALEtVGEKKAF
0 1 K537-u LLFLRYHkEKEVVAV
0 1 Y549-p VAVAHAVyQAVLSLK
0 1 K608-u VFNVDNAkFVVIFDL
0 1 S753-p ETYAPLFsLPSFHKF
0 3 S899 RLDKRDQStIPRNLL
0 3 T900-p LDKRDQStIPRNLLK
0 1 Y995-p ENLEKLMyNAyEGCA
0 1 Y998-p EKLMyNAyEGCANAL
0 1 T1006-p EGCANALtsPPKVIR
0 1 S1007-p GCANALtsPPKVIRT
0 1 S1221-p KKSTSSTsLNLKADF
0 1 K1232-u KADFNYIkSLSSFES
0 1 K1287-a PDPRELQkSIEVQLL
0 1 K1287 PDPRELQKSIEVQLL
0 1 K1513-a SCAISFCkSVkAEyA
0 1 K1516-a ISFCkSVkAEyAVAK
0 1 Y1519-p CkSVkAEyAVAKSIL
0 1 K1632 WAYRWGRKVVDNAsQ
0 1 S1638-p RKVVDNAsQGEGVRL
0 2 S1897-p QGEELLVsECEGGsP
0 4 S1903-p VsECEGGsPPAsQDs
0 4 S1907-p EGGsPPAsQDsNKDE
0 4 S1910-p sPPAsQDsNKDEPKS
0 1 K1989-u QQLEDEVkRVQNNNT
0 3 S2087-p QRAQKRAsYILRLEE
0 1 K2138-a LPTKTKPkkLLFLGS
0 1 K2139-a PTKTKPkkLLFLGSD
0 1 K2148-a LFLGSDGkSYPyLFK
0 1 Y2152-p SDGkSYPyLFKGLED
0 1 S2171 ERIMQFLSIVNTMFA
0 1 T2175 QFLSIVNTMFATINR
0 1 Y2193-p PRFHARHySVTPLGT
0 1 Y2218-p ATPLFGLyKRWQQRE
0 3 T2239-p KAQDSYQtPQNPGIV
0 1 Y2253-p VPRPSELyysKIGPA
0 1 Y2254-p PRPSELyysKIGPAL
0 1 S2255-p RPSELyysKIGPALK
0 1 Y2536-p SHTLQHRysEHTQLQ
0 1 S2537-p HTLQHRysEHTQLQT
0 1 Y2713-p TEMTLQRyAADINSR
0 2 T2776-p ISALCTLtRRNLMME
0 1 S2955-p VDETPKMsAGQMLLV
0 1 T3181-p DLVRRLEtSISSCKT
0 1 K3434 HLLKAMAKDEEAALA
0 1 T3529 TNECSSPTSSATYQP
0 1 S3544-p SFAAAVRsNtGQKtQ
0 11 T3546-p AAAVRsNtGQKtQPD
0 83 T3550-p RsNtGQKtQPDVMsQ
0 77 S3556-p KtQPDVMsQNARKLI
0 2 T3569-p LIQKNLAtsADtPPs
0 21 S3570-p IQKNLAtsADtPPst
0 21 T3573-p NLAtsADtPPstVPG
0 3 S3576-p tsADtPPstVPGTGK
0 7 T3577-p sADtPPstVPGTGKS
  mouse

 
S11-p RAPGSRLssGGGGTK
S12-p APGSRLssGGGGTKY
T17 LssGGGGTKYPRSWN
T30-p WNDWQPRtDsASADP
S32-p DWQPRtDsASADPDT
Y42 ADPDTLKYSSSRDRG
S138 MRKSQERSMSYSDES
S140 KSQERSMSYSDESRL
S145 SMSYSDESRLSNLLR
K171-a LATVKQLkEFIQQPE
Y250 KDEVKLLYLCATYRA
T254 KLLYLCATYRALETV
Y255 LLYLCATYRALETVG
T260 ATYRALETVGEKKAF
K535 LLFLRYHKEKEVVAV
Y547 VAVAHAVYQAVLSLK
N606 VFNIDNANFVVIFDL
S751 ETYAPLFSLPSFHKF
S897-p RLDKRDQstIPRNLL
T898-p LDKRDQstIPRNLLK
Y993 ENLEKLMYNAYEGCA
Y996 EKLMYNAYEGCANAL
T1004 EGCANALTSPPKVIR
S1005 GCANALTSPPKVIRT
S1219 KKNSSSTSLNLKADF
K1230 KADFNYIKSLSSFES
K1285 PDPRELQKSIEVQLL
K1285-u PDPRELQkSIEVQLL
K1511 SCAISFCKSAKAEYA
K1514 ISFCKSAKAEYAVAK
Y1517 CKSAKAEYAVAKSIL
K1630-u WAYRWGRkVVDNASQ
S1636 RkVVDNASQGEGVRL
S1895 QGEELLVSECEGGsP
S1901-p VSECEGGsPPAsQDs
S1905-p EGGsPPAsQDsNKDE
S1908-p sPPAsQDsNKDEPKS
K1987 QQLEDEVKRVQNNNT
S2085 QRAQKRASYILRLDE
K2136 LPTKTKPKKLLFLGS
K2137 PTKTKPKKLLFLGSD
K2146 LFLGSDGKSYPYLFK
Y2150 SDGKSYPYLFKGLED
S2169-p ERIMQFLsIVNtMFA
T2173-p QFLsIVNtMFATINR
Y2191 PRFHARHYSVTPLGT
Y2216 ATPLFGLYKRWQQRE
T2237 KAQDSYQTPQNPSIV
Y2251 VPRPSELYYSKIGPA
Y2252 PRPSELYYSKIGPAL
S2253 RPSELYYSKIGPALK
Y2534 SHTLQHRYSEHTQLQ
S2535 HTLQHRYSEHTQLQT
Y2711 TEMTLQRYAADINSR
T2774 ISALCTLTRRNLMME
S2953 VDETPKMSAGQMLLV
T3179 DLVRRLETSISSCKT
K3431-u HLLKAMAkDEEAALA
T3526-p ANECSSPtSSATYQP
S3541 SFAAAVRSNTGQKtQ
T3543 AAAVRSNTGQKtQPD
T3547-p RSNTGQKtQPDVMsQ
S3553-p KtQPDVMsQNAKKLI
T3566-p LIQKNLAtsADtPPs
S3567-p IQKNLAtsADtPPst
T3570-p NLAtsADtPPstIPG
S3573-p tsADtPPstIPGTGK
T3574-p sADtPPstIPGTGKS
  rat

 
S11 RAPGSRLSSGGGGTK
S12 APGSRLSSGGGGTKY
T17 LSSGGGGTKYPRSWN
T30 WNDWQPRTDSASADP
S32 DWQPRTDSASADPDT
Y42 ADPDTLKYSSSRDRG
S138 MRKSQERSMSYSDES
S140 KSQERSMSYSDESRL
S145 SMSYSDESRLSNLLR
K171-a LATVKQLkEFIQQPE
Y250 KDEVKLLYLCATYRA
T254 KLLYLCATYRALETV
Y255 LLYLCATYRALETVG
T260 ATYRALETVGEKKAF
K535 LLFLRYHKEKEVVAV
Y547 VAVAHAVYQAVLSLK
N606 VFNIDNANFVVIFDL
S751 ETYAPLFSLPSFHKF
S897 RLDKRDQSTIPRNLL
T898 LDKRDQSTIPRNLLK
Y993 ENLEKLMYNAYEGCA
Y996 EKLMYNAYEGCANAL
T1004 EGCANALTSPPKVIR
S1005 GCANALTSPPKVIRT
S1219 KKNANSTSLNLKADF
K1230 KADFNYIKSLSSFES
K1285 PDPRELQKSIEVQLL
K1285 PDPRELQKSIEVQLL
K1511 SCAISFCKSAKAEYA
K1514 ISFCKSAKAEYAVAK
Y1517 CKSAKAEYAVAKSIL
K1630 WAYRWGRKVVDNASQ
S1636 RKVVDNASQGEGVRL
S1895 QGEELLVSECEGGSP
S1901 VSECEGGSPPASQDS
S1905 EGGSPPASQDSNKDE
S1908 SPPASQDSNKDEPKS
K1987 QQLEDEVKRVQNNNT
S2085 QRAQKRASYILRLDE
K2136 LPTKTKPKKLLFLGS
K2137 PTKTKPKKLLFLGSD
K2146 LFLGSDGKSYPYLFK
Y2150 SDGKSYPYLFKGLED
S2169 ERIMQFLSIVNTMFA
T2173 QFLSIVNTMFATINR
Y2191 PRFHARHYSVTPLGT
Y2216 ATPLFGLYKRWQQRE
T2237 KAQDSYQTPQNPSIV
Y2251 VPRPSELYYSKIGPA
Y2252 PRPSELYYSKIGPAL
S2253 RPSELYYSKIGPALK
Y2534 SRTLQHRYSEHTQLQ
S2535 RTLQHRYSEHTQLQT
Y2711 TEMTLQRYAADINSR
T2774 ISALCTLTRRNLMME
S2953 VDETPKMSAGQMLLV
T3179 DLVRRLETSISSCKT
K3431 HLLKAMAKDEEAALA
T3526 ANECSSPTSSATYQP
S3541 SFAAAVRSNTGQKTQ
T3543 AAAVRSNTGQKTQPD
T3547 RSNTGQKTQPDVMSQ
S3553 KTQPDVMSQNAKKLI
T3566-p LIQKNLAtsADtPPs
S3567-p IQKNLAtsADtPPsT
T3570-p NLAtsADtPPsTIPG
S3573-p tsADtPPsTIPGTGK
T3574 sADtPPsTIPGTGKS
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