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Protein Page:
SMG1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
SMG1 Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ. Component of the SMG1C complex composed of SMG1, SMG8 and SMG9; the recruitment of SMG8 to SMG1 N-terminus induces a large conformational change in the SMG1 C-terminal head domain containing the catalytic domain. Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. Interacts with PRKCI. Interacts with TELO2 and TTI1. Interacts with RUVBL1 and RUVBL2. Interacts with UPF2. Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver. Inhibited by caffeine, LY294002 and wortmannin. Belongs to the PI3/PI4-kinase family. 4 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; SMG1 subfamily
Chromosomal Location of Human Ortholog: 16p12.3
Cellular Component: cytoplasm; nucleus; cytosol
Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; metal ion binding; ATP binding; protein kinase activity
Biological Process: mRNA export from nucleus; peptidyl-serine phosphorylation; phosphoinositide phosphorylation; protein amino acid autophosphorylation; mRNA catabolic process, nonsense-mediated decay; response to stress; gene expression; double-strand break repair via homologous recombination
Reference #:  Q96Q15 (UniProtKB)
Alt. Names/Synonyms: 61E3.4; ATX; hSMG-1; KIAA0421; Lambda-interacting protein; Lambda/iota protein kinase C-interacting protein; LIP; phosphatidylinositol 3-kinase-related kinase; phosphatidylinositol 3-kinase-related protein kinase; PI-3-kinase-related kinase SMG-1; Serine/threonine-protein kinase SMG1; SMG-1; SMG1; SMG1 homolog, phosphatidylinositol 3-kinase-related kinase (C. elegans)
Gene Symbols: SMG1
Molecular weight: 410,501 Da
Basal Isoelectric point: 6.03  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

SMG1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S11 RAPGSRLSSGGGGGG
0 1 S12 APGSRLSSGGGGGGt
0 1 T19-p SGGGGGGtKYPRSWN
0 2 T32-p WNDWQPRtDsASADP
0 3 S34-p DWQPRtDsASADPDN
0 1 Y44-p ADPDNLKySSSRDRG
0 1 S94-p GYSVNGGsGENTYGR
0 1 S103-p ENTYGRKsLGQELRV
0 2 S115-p LRVNNVTsPEFTSVQ
0 1 S140-p MRKSQERsMsysDEs
0 3 S142-p KSQERsMsysDEsRL
0 1 Y143-p SQERsMsysDEsRLS
0 1 S144-p QERsMsysDEsRLSN
0 5 S147-p sMsysDEsRLSNLLR
0 1 T157-p SNLLRRItREDDRDR
0 21 K173-ac LATVKQLkEFIQQPE
0 1 Y252-p KDEVKLLyLCAtyKA
0 1 T256-p KLLyLCAtyKALEtV
0 2 Y257-p LLyLCAtyKALEtVG
0 1 T262-p AtyKALEtVGEKKAF
0 1 K529-ub KLFIPSSkLLFLRYH
0 1 K537-ub LLFLRYHkEKEVVAV
0 1 Y549-p VAVAHAVyQAVLSLK
0 1 K598-ub EIKHEAFkNHVFNVD
0 1 K608-ub VFNVDNAkFVVIFDL
0 1 S708-p TATKKHFsIILNLLG
0 1 S753-p ETYAPLFsLPSFHKF
0 3 S899 RLDKRDQStIPRNLL
0 3 T900-p LDKRDQStIPRNLLK
0 1 Y995-p ENLEKLMyNAyEGCA
0 1 Y998-p EKLMyNAyEGCANAL
0 1 T1006-p EGCANALtsPPKVIR
0 1 S1007-p GCANALtsPPKVIRT
0 1 T1047-p LAGQPAVtVRHGFDL
0 1 S1218-p HDLKKSTsSTsLNLK
0 1 S1221-p KKSTsSTsLNLKADF
0 1 K1232-ub KADFNYIkSLSSFES
0 1 K1287-ac PDPRELQkSIEVQLL
0 1 K1287-ub PDPRELQkSIEVQLL
0 1 K1513-ac SCAISFCkSVkAEyA
0 1 K1516-ac ISFCkSVkAEyAVAK
0 1 Y1519-p CkSVkAEyAVAKSIL
0 1 T1527-p AVAKSILtLAKWIQA
0 1 K1632-ub WAYRWGRkVVDNAsQ
0 1 S1638-p RkVVDNAsQGEGVRL
0 1 T1660-p VQNLLPDtITEEEKE
0 1 T1789-p DDMIVMAtLRLLRLL
0 2 S1897-p QGEELLVsECEGGsP
0 5 S1903-p VsECEGGsPPAsQDs
0 5 S1907-p EGGsPPAsQDsNKDE
0 5 S1910-p sPPAsQDsNKDEPKs
0 1 S1917-p sNKDEPKsGLNEDQA
0 1 K1989-ub QQLEDEVkRVQNNNT
0 3 S2087-p QRAQKRAsYILRLEE
0 1 K2138-ac LPTKTKPkkLLFLGS
0 1 K2139-ac PTKTKPkkLLFLGSD
0 1 K2148-ac LFLGSDGksyPyLFK
0 1 S2149-p FLGSDGksyPyLFKG
0 1 Y2150-p LGSDGksyPyLFKGL
0 1 Y2152-p SDGksyPyLFKGLED
0 1 S2171 ERIMQFLSIVNTMFA
0 1 T2175 QFLSIVNTMFATINR
0 1 Y2193-p PRFHARHySVTPLGT
0 3 T2239-p KAQDSYQtPQNPGIV
0 1 Y2253-p VPRPSELyysKIGPA
0 1 Y2254-p PRPSELyysKIGPAL
0 1 S2255-p RPSELyysKIGPALK
0 2 S2364 VCFEKGKSLRVPEKV
0 1 Y2536-p SHTLQHRysEHTQLQ
0 1 S2537-p HTLQHRysEHTQLQT
0 1 Y2713-p TEMTLQRyAADINSR
0 2 T2776-p ISALCTLtRRNLMME
0 1 S2955-p VDETPKMsAGQMLLV
0 1 T3181-p DLVRRLEtSISSCKT
0 1 K3434 HLLKAMAKDEEAALA
0 1 T3529 TNECSSPTSSATYQP
0 1 S3544-p SFAAAVRsNtGQKtQ
0 10 T3546-p AAAVRsNtGQKtQPD
0 92 T3550-p RsNtGQKtQPDVMsQ
0 86 S3556-p KtQPDVMsQNARKLI
0 3 T3569-p LIQKNLAtsADtPPs
0 31 S3570-p IQKNLAtsADtPPst
0 34 T3573-p NLAtsADtPPstVPG
0 10 S3576-p tsADtPPstVPGTGK
0 10 T3577-p sADtPPstVPGTGKS
0 1 S3588 TGKSVACSPKKAVRD
  SMG1 iso2  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S2 ______MSYSDESRL
Y3 _____MSYSDESRLS
S4 ____MSYSDESRLSN
S7 _MSYSDESRLSNLLR
T17 SNLLRRITREDDRDR
K33 LATVKQLKEFIQQPE
Y112 KDEVKLLYLCATYKA
T116 KLLYLCATYKALETV
Y117 LLYLCATYKALETVG
T122 ATYKALETVGEKKAF
K389 KLFIPSSKLLFLRYH
K397 LLFLRYHKEKEVVAV
Y409 VAVAHAVYQAVLSLK
K458 EIKHEAFKNHVFNVD
K468 VFNVDNAKFVVIFDL
S568 TATKKHFSIILNLLG
S613 ETYAPLFSLPSFHKF
S759 RLDKRDQSTIPRNLL
T760 LDKRDQSTIPRNLLK
Y855 ENLEKLMYNAYEGCA
Y858 EKLMYNAYEGCANAL
T866 EGCANALTSPPKVIR
S867 GCANALTSPPKVIRT
T907 LAGQPAVTVRHGFDL
S1078 HDLKKSTSSTSLNLK
S1081 KKSTSSTSLNLKADF
K1092 KADFNYIKSLSSFES
K1147 PDPRELQKSIEVQLL
K1147 PDPRELQKSIEVQLL
K1373 SCAISFCKSVKAEYA
K1376 ISFCKSVKAEYAVAK
Y1379 CKSVKAEYAVAKSIL
T1387 AVAKSILTLAKWIQA
K1492 WAYRWGRKVVDNASQ
S1498 RKVVDNASQGEGVRL
T1520 VQNLLPDTITEEEKE
T1649 DDMIVMATLRLLRLL
S1757 QGEELLVSECEGGSP
S1763 VSECEGGSPPASQDS
S1767 EGGSPPASQDSNKDE
S1770 SPPASQDSNKDEPKS
S1777 SNKDEPKSGLNEDQA
K1849 QQLEDEVKRVQNNNT
S1947 QRAQKRASYILRLEE
K1998 LPTKTKPKKLLFLGS
K1999 PTKTKPKKLLFLGSD
K2008 LFLGSDGKSYPYLFK
S2009 FLGSDGKSYPYLFKG
Y2010 LGSDGKSYPYLFKGL
Y2012 SDGKSYPYLFKGLED
S2031 ERIMQFLSIVNTMFA
T2035 QFLSIVNTMFATINR
Y2053 PRFHARHYSVTPLGT
T2099 KAQDSYQTPQNPGIV
Y2113 VPRPSELYYSKIGPA
Y2114 PRPSELYYSKIGPAL
S2115 RPSELYYSKIGPALK
S2224 VCFEKGKSLRVPEKV
Y2396 SHTLQHRYSEHTQLQ
S2397 HTLQHRYSEHTQLQT
Y2573 TEMTLQRYAADINSR
T2636 ISALCTLTRRNLMME
S2815 VDETPKMSAGQMLLV
T3041 DLVRRLETSISSCKT
K3294 HLLKAMAKDEEAALA
T3389 TNECSSPTSSATYQP
S3404 SFAAAVRSNTGQKTQ
T3406 AAAVRSNTGQKTQPD
T3410 RSNTGQKTQPDVMSQ
S3416 KTQPDVMSQNARKLI
T3429 LIQKNLATSADTPPS
S3430 IQKNLATSADTPPST
T3433 NLATSADTPPSTVPG
S3436 TSADTPPSTVPGTGK
T3437 SADTPPSTVPGTGKS
S3448 TGKSVACSPKKAVRD
  mouse

 
S11-p RAPGSRLssGGGGTK
S12-p APGSRLssGGGGTKY
T17 LssGGGGTKYPRSWN
T30-p WNDWQPRtDsASADP
S32-p DWQPRtDsASADPDT
Y42 ADPDTLKYSSSRDRG
S92 GYSVNGGSGENTYGR
S101 ENTYGRKSLGQELRI
S113 LRINNVTSPEFTSVQ
S138 MRKSQERSMSYSDES
S140 KSQERSMSYSDESRL
Y141 SQERSMSYSDESRLS
S142 QERSMSYSDESRLSN
S145 SMSYSDESRLSNLLR
T155 SNLLRRITREDDRDR
K171-ac LATVKQLkEFIQQPE
Y250 KDEVKLLYLCATYRA
T254 KLLYLCATYRALETV
Y255 LLYLCATYRALETVG
T260 ATYRALETVGEKKAF
K527 KLFIPSSKLLFLRYH
K535 LLFLRYHKEKEVVAV
Y547 VAVAHAVYQAVLSLK
Q596 EIKHEAFQNHVFNID
N606 VFNIDNANFVVIFDL
S706 TATKKHFSIILNLLG
S751 ETYAPLFSLPSFHKF
S897-p RLDKRDQstIPRNLL
T898-p LDKRDQstIPRNLLK
Y993 ENLEKLMYNAYEGCA
Y996 EKLMYNAYEGCANAL
T1004 EGCANALTSPPKVIR
S1005 GCANALTSPPKVIRT
T1045 LAGQPAVTVRHGFDL
S1216 HDLKKNSSSTSLNLK
S1219 KKNSSSTSLNLKADF
K1230 KADFNYIKSLSSFES
K1285 PDPRELQKSIEVQLL
K1285-ub PDPRELQkSIEVQLL
K1511 SCAISFCKSAKAEYA
K1514 ISFCKSAKAEYAVAK
Y1517 CKSAKAEYAVAKSIL
T1525 AVAKSILTLAKWVQA
K1630-ub WAYRWGRkVVDNASQ
S1636 RkVVDNASQGEGVRL
T1658 VQNLLPDTITEEEKE
T1787 DDVIVMATLRLLRLL
S1895 QGEELLVSECEGGsP
S1901-p VSECEGGsPPAsQDs
S1905-p EGGsPPAsQDsNKDE
S1908-p sPPAsQDsNKDEPKS
S1915 sNKDEPKSGLNEDQA
K1987 QQLEDEVKRVQNNNT
S2085 QRAQKRASYILRLDE
K2136 LPTKTKPKKLLFLGS
K2137 PTKTKPKKLLFLGSD
K2146 LFLGSDGKSYPYLFK
S2147 FLGSDGKSYPYLFKG
Y2148 LGSDGKSYPYLFKGL
Y2150 SDGKSYPYLFKGLED
S2169-p ERIMQFLsIVNtMFA
T2173-p QFLsIVNtMFATINR
Y2191 PRFHARHYSVTPLGT
T2237 KAQDSYQTPQNPSIV
Y2251 VPRPSELYYSKIGPA
Y2252 PRPSELYYSKIGPAL
S2253 RPSELYYSKIGPALK
S2362-p VCFEKGKsLRVPEKV
Y2534 SHTLQHRYSEHTQLQ
S2535 HTLQHRYSEHTQLQT
Y2711 TEMTLQRYAADINSR
T2774 ISALCTLTRRNLMME
S2953 VDETPKMSAGQMLLV
T3179 DLVRRLETSISSCKT
K3431-ub HLLKAMAkDEEAALA
T3526-p ANECSSPtSSATYQP
S3541 SFAAAVRSNTGQKtQ
T3543 AAAVRSNTGQKtQPD
T3547-p RSNTGQKtQPDVMsQ
S3553-p KtQPDVMsQNAKKLI
T3566-p LIQKNLAtsADtPPs
S3567-p IQKNLAtsADtPPst
T3570-p NLAtsADtPPstIPG
S3573-p tsADtPPstIPGTGK
T3574-p sADtPPstIPGTGKS
S3585-p TGKSIACsPKKAVRD
  rat

 
S11 RAPGSRLSSGGGGTK
S12 APGSRLSSGGGGTKY
T17 LSSGGGGTKYPRSWN
T30 WNDWQPRTDSASADP
S32 DWQPRTDSASADPDT
Y42 ADPDTLKYSSSRDRG
S92 GYSVNGGSGENTYGR
S101 ENTYGRKSLGQELRI
S113 LRINNVTSPEFTSVQ
S138 MRKSQERSMSYSDES
S140 KSQERSMSYSDESRL
Y141 SQERSMSYSDESRLS
S142 QERSMSYSDESRLSN
S145 SMSYSDESRLSNLLR
T155 SNLLRRITREDDRDR
K171-ac LATVKQLkEFIQQPE
Y250 KDEVKLLYLCATYRA
T254 KLLYLCATYRALETV
Y255 LLYLCATYRALETVG
T260 ATYRALETVGEKKAF
K527 KLFIPSSKLLFLRYH
K535 LLFLRYHKEKEVVAV
Y547 VAVAHAVYQAVLSLK
Q596 EIKHEAFQNHVFNID
N606 VFNIDNANFVVIFDL
S706 TATKKHFSIILNLLG
S751 ETYAPLFSLPSFHKF
S897 RLDKRDQSTIPRNLL
T898 LDKRDQSTIPRNLLK
Y993 ENLEKLMYNAYEGCA
Y996 EKLMYNAYEGCANAL
T1004 EGCANALTSPPKVIR
S1005 GCANALTSPPKVIRT
T1045 LAGQPAVTVRHGFDL
N1216 HDLKKNANSTSLNLK
S1219 KKNANSTSLNLKADF
K1230 KADFNYIKSLSSFES
K1285 PDPRELQKSIEVQLL
K1285 PDPRELQKSIEVQLL
K1511 SCAISFCKSAKAEYA
K1514 ISFCKSAKAEYAVAK
Y1517 CKSAKAEYAVAKSIL
T1525 AVAKSILTLAKWVQA
K1630 WAYRWGRKVVDNASQ
S1636 RKVVDNASQGEGVRL
T1658 VQNLLPDTITEEEKE
T1787 DDVIVMATLRLLRLL
S1895 QGEELLVSECEGGSP
S1901 VSECEGGSPPASQDS
S1905 EGGSPPASQDSNKDE
S1908 SPPASQDSNKDEPKS
S1915 SNKDEPKSGLNEDQA
K1987 QQLEDEVKRVQNNNT
S2085 QRAQKRASYILRLDE
K2136 LPTKTKPKKLLFLGS
K2137 PTKTKPKKLLFLGSD
K2146 LFLGSDGKSYPYLFK
S2147 FLGSDGKSYPYLFKG
Y2148 LGSDGKSYPYLFKGL
Y2150 SDGKSYPYLFKGLED
S2169 ERIMQFLSIVNTMFA
T2173 QFLSIVNTMFATINR
Y2191 PRFHARHYSVTPLGT
T2237 KAQDSYQTPQNPSIV
Y2251 VPRPSELYYSKIGPA
Y2252 PRPSELYYSKIGPAL
S2253 RPSELYYSKIGPALK
S2362 VCFEKGKSLRVPEKV
Y2534 SRTLQHRYSEHTQLQ
S2535 RTLQHRYSEHTQLQT
Y2711 TEMTLQRYAADINSR
T2774 ISALCTLTRRNLMME
S2953 VDETPKMSAGQMLLV
T3179 DLVRRLETSISSCKT
K3431 HLLKAMAKDEEAALA
T3526 ANECSSPTSSATYQP
S3541 SFAAAVRSNTGQKTQ
T3543 AAAVRSNTGQKTQPD
T3547 RSNTGQKTQPDVMSQ
S3553 KTQPDVMSQNAKKLI
T3566-p LIQKNLAtsADtPPs
S3567-p IQKNLAtsADtPPsT
T3570-p NLAtsADtPPsTIPG
S3573-p tsADtPPsTIPGTGK
T3574 sADtPPsTIPGTGKS
S3585 TGKSIACSPKKAIRD
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