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Protein Page:
BRD4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BRD4 an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, atypical; ATYPICAL group; BRD family
Cellular Component: condensed nuclear chromosome; cytoplasm; nuclear chromatin; nucleolus; chromosome; nucleus
Molecular Function: protein binding; DNA binding; p53 binding; chromatin binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; viral reproduction; inner cell mass cell proliferation; protein amino acid phosphorylation; chromosome segregation; G1/S-specific transcription in mitotic cell cycle; chromatin remodeling; positive regulation of RNA elongation from RNA polymerase II promoter; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of DNA binding
Reference #:  O60885 (UniProtKB)
Alt. Names/Synonyms: BRD4; bromodomain containing 4; bromodomain-containing 4; Bromodomain-containing protein 4; CAP; chromosome-associated protein; HUNK1; HUNKI; MCAP; Protein HUNK1
Gene Symbols: BRD4
Molecular weight: 152,219 Da
Basal Isoelectric point: 9.23  Predict pI for various phosphorylation states
CST Pathways:  Crosstalk between PTMs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BRD4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 Y65-p RQTNQLQyLLRVVLK
0 1 K99 LNLPDYYKIIKtPMD
0 1 T103-p DYYKIIKtPMDMGtI
0 1 T109-p KtPMDMGtIkKRLEN
0 1 K111-ac PMDMGtIkKRLENNY
0 1 T294 GVKRKADTTTPTtID
0 1 T296 KRKADTTTPTtIDPI
0 1 T299-p ADTTTPTtIDPIHEP
0 1 T315-p SLPPEPKttKLGQRR
0 1 T316-p LPPEPKttKLGQRRE
0 9 S324-p KLGQRREssRPVKPP
0 4 S325-p LGQRREssRPVKPPK
0 1 K333-ac RPVKPPKkDVPdSQQ
0 1 D337-ca PPKkDVPdSQQHPAP
0 1 K346-ac QQHPAPEkSSKVSEQ
0 1 K362-ac KCCSGILkEMFAkkH
0 1 K367-ac ILkEMFAkkHAAYAW
0 1 K368-ac LkEMFAkkHAAYAWP
0 1 K404-m2 PMDMSTIkSkLEARE
0 1 K406-m2 DMSTIkSkLEAREYR
0 1 A467 EPEEPVVAVssPAVP
0 1 S469-p EEPVVAVssPAVPPP
0 13 S470-p EPVVAVssPAVPPPT
1 0 S484-p TKVVAPPsSSDsSSD
1 0 S488-p APPsSSDsSSDsSsD
1 0 S492-p SSDsSSDsSsDSDss
1 0 S494-p DsSSDsSsDSDssTD
1 0 S498-p DsSsDSDssTDDsEE
1 0 S499-p sSsDSDssTDDsEEE
1 0 S503-p SDssTDDsEEERAQR
0 2 S578-p KKTKKNNssNsNVsK
0 2 S579-p KTKKNNssNsNVsKK
0 3 S581-p KKNNssNsNVsKKEP
0 1 S584-p NssNsNVsKKEPAPM
0 2 T598-p MKSKPPPtyEsEEED
0 2 Y599-p KSKPPPtyEsEEEDK
0 16 S601-p KPPPtyEsEEEDKCK
0 1 S619-p YEEKRQLsLDINKLP
0 1 S673-p ELERYVTsCLRKKRK
0 1 S701 KMKGFSSSESEsSSE
0 1 S705-p FSSSESEsSSESSSS
0 1 S706 SSSESEsSSESSSSD
0 1 S711 EsSSESSSSDSEDSE
0 1 S714 SESSSSDSEDSETEM
0 1 S717 SSSDSEDSETEMAPK
0 2 K726-ac TEMAPKSkkKGHPGR
0 2 K727-ac EMAPKSkkKGHPGRE
0 1 S858-p LNQHAVVsPPALHNA
0 2 T942-p LQKVQPPtPLLPSVK
0 5 S1045-p QVIQHHHsPRHHKSD
0 1 Y1054-p RHHKSDPySTGHLRE
0 9 S1064-p GHLREAPsPLMIHsP
0 7 S1070-p PsPLMIHsPQMsQFQ
0 1 S1074-p MIHsPQMsQFQSLtH
0 3 T1080-p MsQFQSLtHQsPPQQ
0 4 S1083-p FQSLtHQsPPQQNVQ
0 7 K1111-ac PQPLVVVkEEkIHsP
0 2 K1114-ac LVVVkEEkIHsPIIR
0 33 S1117-p VkEEkIHsPIIRSEP
0 5 S1126-p IIRSEPFsPSLRPEP
0 9 S1201-p LKIKNMGsWAsLVQK
0 2 S1204-p KNMGsWAsLVQKHPt
0 2 T1211-p sLVQKHPttPSsTAK
0 3 T1212-p LVQKHPttPSsTAKS
0 1 S1215-gl KHPttPSsTAKSSSD
0 1 S1319 AQSSQPQSMLDQQRE
  BRD4 iso2  
Y65 RQTNQLQYLLRVVLK
K99 LNLPDYYKIIKTPMD
T103 DYYKIIKTPMDMGTI
T109 KTPMDMGTIKKRLEN
K111 PMDMGTIKKRLENNY
T294 GVKRKADTTTPTTID
T296 KRKADTTTPTTIDPI
T299 ADTTTPTTIDPIHEP
T315 SLPPEPKTTKLGQRR
T316 LPPEPKTTKLGQRRE
S324 KLGQRRESSRPVKPP
S325 LGQRRESSRPVKPPK
K333 RPVKPPKKDVPDSQQ
D337 PPKKDVPDSQQHPAP
K346 QQHPAPEKSSKVSEQ
K362 KCCSGILKEMFAKKH
K367 ILKEMFAKKHAAYAW
K368 LKEMFAKKHAAYAWP
K404 PMDMSTIKSKLEARE
K406 DMSTIKSKLEAREYR
A467 EPEEPVVAVSSPAVP
S469 EEPVVAVSSPAVPPP
S470 EPVVAVSSPAVPPPT
S484 TKVVAPPSSSDSSSD
S488 APPSSSDSSSDSSSD
S492 SSDSSSDSSSDSDSS
S494 DSSSDSSSDSDSSTD
S498 DSSSDSDSSTDDSEE
S499 SSSDSDSSTDDSEEE
S503 SDSSTDDSEEERAQR
S578 KKTKKNNSSNSNVSK
S579 KTKKNNSSNSNVSKK
S581 KKNNSSNSNVSKKEP
S584 NSSNSNVSKKEPAPM
T598 MKSKPPPTYESEEED
Y599 KSKPPPTYESEEEDK
S601 KPPPTYESEEEDKCK
S619 YEEKRQLSLDINKLP
S673 ELERYVTSCLRKKRK
S701-p KMKGFSSsESESsSE
S705 FSSsESESsSESSsS
S706-p SSsESESsSESSsSD
S711-p ESsSESSsSDSEDSE
S714 SESSsSDSEDSETGP
S717 SsSDSEDSETGPA__
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  BRD4 iso3  
Y65 RQTNQLQYLLRVVLK
K99 LNLPDYYKIIKTPMD
T103 DYYKIIKTPMDMGTI
T109 KTPMDMGTIKKRLEN
K111 PMDMGTIKKRLENNY
T294 GVKRKADTTTPTTID
T296 KRKADTTTPTTIDPI
T299 ADTTTPTTIDPIHEP
T315 SLPPEPKTTKLGQRR
T316 LPPEPKTTKLGQRRE
S324 KLGQRRESSRPVKPP
S325 LGQRRESSRPVKPPK
K333 RPVKPPKKDVPDSQQ
D337 PPKKDVPDSQQHPAP
K346 QQHPAPEKSSKVSEQ
K362 KCCSGILKEMFAKKH
K367 ILKEMFAKKHAAYAW
K368 LKEMFAKKHAAYAWP
K404 PMDMSTIKSKLEARE
K406 DMSTIKSKLEAREYR
A467 EPEEPVVAVSSPAVP
S469 EEPVVAVSSPAVPPP
S470 EPVVAVSSPAVPPPT
S484 TKVVAPPSSSDSSSD
S488 APPSSSDSSSDSSSD
S492 SSDSSSDSSSDSDSS
S494 DSSSDSSSDSDSSTD
S498 DSSSDSDSSTDDSEE
S499 SSSDSDSSTDDSEEE
S503 SDSSTDDSEEERAQR
S578 KKTKKNNSSNSNVSK
S579 KTKKNNSSNSNVSKK
S581 KKNNSSNSNVSKKEP
S584 NSSNSNVSKKEPAPM
T598 MKSKPPPTYESEEED
Y599 KSKPPPTYESEEEDK
S601 KPPPTYESEEEDKCK
S619 YEEKRQLSLDINKLP
S673 ELERYVTSCLRKKRK
S701 KMKGFSSSESESSSE
S705 FSSSESESSSESSSS
S706 SSSESESSSESSSSD
S711 ESSSESSSSDSEDSE
S714 SESSSSDSEDSETAF
S717 SSSDSEDSETAFCTS
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- gap
- gap
- gap
  mouse

 
Y65 RQTNQLQYLLRVVLK
K99-ub LNLPDYYkIIKTPMD
T103 DYYkIIKTPMDMGTI
T109 KTPMDMGTIKKRLEN
K111 PMDMGTIKKRLENNY
T295-p GVKRKADtTtPTTID
T297-p KRKADtTtPTTIDPI
T300 ADtTtPTTIDPIHEP
T316 SLAPEPKTAKLGPRR
A317 LAPEPKTAKLGPRRE
S325-p KLGPRREssRPVKPP
S326-p LGPRREssRPVKPPK
K334 RPVKPPKKDVPDSQQ
D338 PPKKDVPDSQQHPGP
K347 QQHPGPEKSSKISEQ
K363 KCCSGILKEMFAKKH
K368 ILKEMFAKKHAAYAW
K369 LKEMFAKKHAAYAWP
K405 PMDMSTIKSKLESRE
K407 DMSTIKSKLESREYR
T468-p EPEEPVVtVSsPAVP
S470 EEPVVtVSsPAVPPP
S471-p EPVVtVSsPAVPPPT
S485 TKVVAPPSSSDSSSD
S489 APPSSSDSSSDSSSD
S493 SSDSSSDSSSDSDSS
S495 DSSSDSSSDSDSSTD
S499 DSSSDSDSSTDDSEE
S500 SSSDSDSSTDDSEEE
S504 SDSSTDDSEEERAQR
S579 KKTKKNNSSNSNVSK
S580 KTKKNNSSNSNVSKK
S582 KKNNSSNSNVSKKEP
S585 NSSNSNVSKKEPVPT
T599-p TKTKPPPtYEsEEED
Y600 KTKPPPtYEsEEEDK
S602-p KPPPtYEsEEEDKCK
S620 YEEKRQLSLDINKLP
S674 ELERYVTSCLRKKRK
S702 KMKGFSSSESESTSE
S706 FSSSESESTSESSSS
T707 SSSESESTSESSSSD
S712 ESTSESSSSDsEDsE
S715-p SESSSSDsEDsETEM
S718-p SSSDsEDsETEMAPK
K727 TEMAPKSKKKGHTGR
K728 EMAPKSKKKGHTGRD
S860 LNQHAVVSPPALHNA
T944 LQKVQPPTPLLPSVK
S1048-p QVIQHHPsPRHHKSD
Y1057 RHHKSDPYSAGHLRE
S1067-p GHLREAPsPLMIHsP
S1073-p PsPLMIHsPQMPQFQ
P1077 MIHsPQMPQFQSLtH
T1083-p MPQFQSLtHQsPPQQ
S1086-p FQSLtHQsPPQQNVQ
K1147 PQPLVVVKEEKIHsP
K1150 LVVVKEEKIHsPIIR
S1153-p VKEEKIHsPIIRSEP
S1162 IIRSEPFSTSLRPEP
S1237-p LKIKNMGsWASLVQK
S1240 KNMGsWASLVQKHPt
T1247-p SLVQKHPtTPSSTAK
T1248 LVQKHPtTPSSTAKS
S1251 KHPtTPSSTAKSSSD
S1357-p AQSSQPQsMLDQQRE
  rat

 
Y65 RQTNQLQYLLRVVLK
K99 LNLPDYYKIIKTPMD
T103 DYYKIIKTPMDMGTI
T109 KTPMDMGTIKKRLEN
K111 PMDMGTIKKRLENNY
T295 GVKRKADTTTPTTID
T297 KRKADTTTPTTIDPI
T300 ADTTTPTTIDPIHEP
T316 SLAPEPKTAKLGPRR
A317 LAPEPKTAKLGPRRE
S325 KLGPRRESSRPVKPP
S326 LGPRRESSRPVKPPK
K334 RPVKPPKKDVPDSQQ
D338 PPKKDVPDSQQHPGP
K347 QQHPGPEKSSKISEQ
K363 KCCSGILKEMFAKKH
K368 ILKEMFAKKHAAYAW
K369 LKEMFAKKHAAYAWP
K405 PMDMSTIKSKLESRE
K407 DMSTIKSKLESREYR
T468 EPEEPVVTVSSPAVP
S470 EEPVVTVSSPAVPPP
S471 EPVVTVSSPAVPPPT
S485 TKVVAPPSSSDSSSD
S489 APPSSSDSSSDSSSD
S493 SSDSSSDSSSDSDSS
S495 DSSSDSSSDSDSSTD
S499 DSSSDSDSSTDDSEE
S500 SSSDSDSSTDDSEEE
S504 SDSSTDDSEEERAQR
S579 KKTKKNNSSNSNVSK
S580 KTKKNNSSNSNVSKK
S582 KKNNSSNSNVSKKEP
S585 NSSNSNVSKKEPAPT
T599 TKTKPPPTYESEEED
Y600 KTKPPPTYESEEEDK
S602 KPPPTYESEEEDKCK
S620 YEEKRQLSLDINKLP
S674 ELERYVTSCLRKKRK
S702 KMKGFSSSESESTSE
S706 FSSSESESTSESSSS
T707 SSSESESTSESSSSD
S712 ESTSESSSSDSEDSE
S715 SESSSSDSEDSETEM
S718 SSSDSEDSETEMAPK
K727 TEMAPKSKKKGHTGR
K728 EMAPKSKKKGHTGRD
S864 LNQHAVVSPPALHNA
T948 LQKVQPPTPLLPSVK
S1052 QVIQHHPSPRHHKSD
Y1061 RHHKSDPYSAGHLRE
S1071 GHLREAPSPLMIHSP
S1077 PSPLMIHSPQMPQFQ
P1081 MIHSPQMPQFQSLTH
T1087 MPQFQSLTHQSPPQQ
S1090 FQSLTHQSPPQQNVQ
K1151 PQPLVVVKEEKIHsP
K1154 LVVVKEEKIHsPIIR
S1157-p VKEEKIHsPIIRSEP
S1166 IIRSEPFSTSLRPEP
S1241 LKIKNMGSWASLVQK
S1244 KNMGSWASLVQKHPT
T1251 SLVQKHPTTPSSTAK
T1252 LVQKHPTTPSSTAKS
S1255 KHPTTPSSTAKSSSD
S1360 AQSSQPQSMLDQQRE
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