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BRD4 (human)

Overview BRD4 an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB. Protein type: Protein kinase, ATYPICAL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; BRD family Cellular Component: condensed nuclear chromosome; cytoplasm; nuclear chromatin; nucleolus; nucleus Molecular Function: protein binding; DNA binding Biological Process: G1-specific transcription in mitotic cell cycle; positive regulation of RNA elongation from RNA polymerase II promoter; virus-host interaction; inner cell mass cell proliferation; protein amino acid phosphorylation; chromosome segregation; positive regulation of DNA binding Reference #:  O60885 (UniProtKB) Alt. Names/Synonyms: BRD4; bromodomain containing 4; bromodomain-containing 4; Bromodomain-containing protein 4; CAP; chromosome-associated protein; HUNK1; HUNKI; MCAP; Protein HUNK1 Gene Symbols: BRD4 Molecular weight: 152,219 Da Basal Isoelectric point: 9.23  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below BRD4 2I8N - A=352-457 (human) 2LSP - B=333-460 (human) 2NNU - B=1342-1362 (human) 2OSS - A=42-168 (human) 2OUO - A=333-460 (human) 2YEL - A=44-168 (human) 2YEM - A/B=333-460 (human) 3P5O - A=44-168 (human) 3SVF - A=44-168 (human) 3SVG - A=44-168 (human) 3U5J - A=42-168 (human) 3U5K - A/D/C/B=42-168 (human) 3U5L - A=42-168 (human) 3UVW - A=44-168 (human) 3UVX - A=44-168 (human) 3UVY - A=44-168 (human) 3UW9 - A/D/C/B=44-168 (human) 3ZYU - A/B=44-168 (human) 4A9L - A=44-168 (human) 4DON - A=44-166 (human) 4E96 - A=44-168 (human) 4F3I - A=44-168 (human) 4GPJ - A=44-168 (human) 4HBV - A=42-168 (human) 4HBW - A=42-168 (human) 4HBX - A=42-168 (human) 4HBY - A=42-168 (human) 4HXK - A=44-167 (human) 4HXL - A=44-167 (human) 4HXM - A=44-166 (human) 4HXN - A=44-167 (human) 4HXO - A=44-167 (human) 4HXP - A=44-166 (human) 4HXR - A=44-167 (human) 4HXS - A=44-166 (human) 4J0R - A=44-168 (human) 4J0S - A=44-168 (human) 2DWW - A=347-460 (mouse) 2JNS - A=601-683 (mouse) 3JVJ - A=42-168 (mouse) 3JVK - A=42-168 (mouse) 3JVL - A=349-464 (mouse) 3JVM - A=349-464 (mouse) 3MUK - A=42-168 (mouse) 3MUL - A=42-168 (mouse) 3MXF - A=44-168 (mouse)

STRING  |  Scansite  |  KinBase  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 2I8N 2LSP 2NNU 2OSS 2OUO 2YEL 2YEM 3P5O 3SVF 3SVG 3U5J 3U5K 3U5L 3UVW 3UVX 3UVY 3UW9 3ZYU 4A9L 4DON 4E96 4F3I 4GPJ 4HBV 4HBW 4HBX 4HBY 4HXK 4HXL 4HXM 4HXN 4HXO 4HXP 4HXR 4HXS 4J0R 4J0S  |  Phospho3D  |  DISEASE  |  Source  |  UCSD-Nature  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human ► Hide Isoforms
0	1		Y65-p	RQTNQLQyLLRVVLK
0	1		K99	LNLPDYYKIIKtPMD
0	1		T103-p	DYYKIIKtPMDMGtI
0	1		T109-p	KtPMDMGtIkKRLEN
0	1		K111-a	PMDMGtIkKRLENNY
0	1		T299-p	ADTTTPTtIDPIHEP
0	1		T315-p	SLPPEPKttKLGQRR
0	1		T316-p	LPPEPKttKLGQRRE
0	8		S324-p	KLGQRREssRPVKPP
0	4		S325-p	LGQRREssRPVKPPK
0	1		K333-a	RPVKPPKkDVPdSQQ
0	1		D337-ca	PPKkDVPdSQQHPAP
0	1		K346-a	QQHPAPEkSSKVSEQ
0	1		K362-a	KCCSGILkEMFAkkH
0	1		K367-a	ILkEMFAkkHAAYAW
0	1		K368-a	LkEMFAkkHAAYAWP
0	1		K404-m2	PMDMSTIkSkLEARE
0	1		K406-m2	DMSTIkSkLEAREYR
0	1		S469-p	EEPVVAVssPAVPPP
0	10		S470-p	EPVVAVssPAVPPPT
1	0		S484-p	TKVVAPPsSSDsSSD
1	0		S488-p	APPsSSDsSSDsSsD
1	0		S492-p	SSDsSSDsSsDSDss
1	0		S494-p	DsSSDsSsDSDssTD
1	0		S498-p	DsSsDSDssTDDsEE
1	0		S499-p	sSsDSDssTDDsEEE
1	0		S503-p	SDssTDDsEEERAQR
0	1		S578-p	KKTKKNNssNsNVsK
0	1		S579-p	KTKKNNssNsNVsKK
0	2		S581-p	KKNNssNsNVsKKEP
0	1		S584-p	NssNsNVsKKEPAPM
0	2		T598	MKSKPPPTyEsEEED
0	2		Y599-p	KSKPPPTyEsEEEDK
0	13		S601-p	KPPPTyEsEEEDKCK
0	1		S619-p	YEEKRQLsLDINKLP
0	1		S705-p	FSSSESEsSSESSSS
0	1		S714	SESSSSDSEDSETEM
0	1		S717	SSSDSEDSETEMAPK
0	2		K726-a	TEMAPKSkkKGHPGR
0	2		K727-a	EMAPKSkkKGHPGRE
0	1		S858-p	LNQHAVVsPPALHNA
0	2		T942-p	LQKVQPPtPLLPSVK
0	4		S1045-p	QVIQHHHsPRHHKSD
0	1		Y1054-p	RHHKSDPySTGHLRE
0	8		S1064-p	GHLREAPsPLMIHsP
0	6		S1070-p	PsPLMIHsPQMSQFQ
0	2		T1080-p	MSQFQSLtHQsPPQQ
0	3		S1083-p	FQSLtHQsPPQQNVQ
0	6		K1111-a	PQPLVVVkEEkIHsP
0	2		K1114-a	LVVVkEEkIHsPIIR
0	24		S1117-p	VkEEkIHsPIIRSEP
0	2		S1126-p	IIRSEPFsPSLRPEP
0	7		S1201-p	LKIKNMGsWASLVQK
0	1		T1211	SLVQKHPTtPSsTAK
0	2		T1212-p	LVQKHPTtPSsTAKS
0	1		S1215-g	KHPTtPSsTAKSSSD

	BRD4 iso2
Y65	RQTNQLQYLLRVVLK
K99	LNLPDYYKIIKTPMD
T103	DYYKIIKTPMDMGTI
T109	KTPMDMGTIKKRLEN
K111	PMDMGTIKKRLENNY
T299	ADTTTPTTIDPIHEP
T315	SLPPEPKTTKLGQRR
T316	LPPEPKTTKLGQRRE
S324	KLGQRRESSRPVKPP
S325	LGQRRESSRPVKPPK
K333	RPVKPPKKDVPDSQQ
D337	PPKKDVPDSQQHPAP
K346	QQHPAPEKSSKVSEQ
K362	KCCSGILKEMFAKKH
K367	ILKEMFAKKHAAYAW
K368	LKEMFAKKHAAYAWP
K404	PMDMSTIKSKLEARE
K406	DMSTIKSKLEAREYR
S469	EEPVVAVSSPAVPPP
S470	EPVVAVSSPAVPPPT
S484	TKVVAPPSSSDSSSD
S488	APPSSSDSSSDSSSD
S492	SSDSSSDSSSDSDSS
S494	DSSSDSSSDSDSSTD
S498	DSSSDSDSSTDDSEE
S499	SSSDSDSSTDDSEEE
S503	SDSSTDDSEEERAQR
S578	KKTKKNNSSNSNVSK
S579	KTKKNNSSNSNVSKK
S581	KKNNSSNSNVSKKEP
S584	NSSNSNVSKKEPAPM
T598	MKSKPPPTYESEEED
Y599	KSKPPPTYESEEEDK
S601	KPPPTYESEEEDKCK
S619	YEEKRQLSLDINKLP
S705	FSSSESESSSESSSS
S714	SESSSSDSEDSETGP
S717	SSSDSEDSETGPA__
-	gap
-	gap
-	gap
-	gap
-	gap
-	under review
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap

	BRD4 iso3
Y65	RQTNQLQYLLRVVLK
K99	LNLPDYYKIIKTPMD
T103	DYYKIIKTPMDMGTI
T109	KTPMDMGTIKKRLEN
K111	PMDMGTIKKRLENNY
T299	ADTTTPTTIDPIHEP
T315	SLPPEPKTTKLGQRR
T316	LPPEPKTTKLGQRRE
S324	KLGQRRESSRPVKPP
S325	LGQRRESSRPVKPPK
K333	RPVKPPKKDVPDSQQ
D337	PPKKDVPDSQQHPAP
K346	QQHPAPEKSSKVSEQ
K362	KCCSGILKEMFAKKH
K367	ILKEMFAKKHAAYAW
K368	LKEMFAKKHAAYAWP
K404	PMDMSTIKSKLEARE
K406	DMSTIKSKLEAREYR
S469	EEPVVAVSSPAVPPP
S470	EPVVAVSSPAVPPPT
S484	TKVVAPPSSSDSSSD
S488	APPSSSDSSSDSSSD
S492	SSDSSSDSSSDSDSS
S494	DSSSDSSSDSDSSTD
S498	DSSSDSDSSTDDSEE
S499	SSSDSDSSTDDSEEE
S503	SDSSTDDSEEERAQR
S578	KKTKKNNSSNSNVSK
S579	KTKKNNSSNSNVSKK
S581	KKNNSSNSNVSKKEP
S584	NSSNSNVSKKEPAPM
T598	MKSKPPPTYESEEED
Y599	KSKPPPTYESEEEDK
S601	KPPPTYESEEEDKCK
S619	YEEKRQLSLDINKLP
S705	FSSSESESSSESSSS
S714	SESSSSDSEDSETAF
S717	SSSDSEDSETAFCTS
-	gap
-	gap
-	gap
-	gap
-	gap
-	under review
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap
-	gap

	mouse
Y65	RQTNQLQYLLRVVLK
K99-u	LNLPDYYkIIKTPMD
T103	DYYkIIKTPMDMGTI
T109	KTPMDMGTIKKRLEN
K111	PMDMGTIKKRLENNY
T300	ADTTTPTTIDPIHEP
T316	SLAPEPKTAKLGPRR
A317	LAPEPKTAKLGPRRE
S325-p	KLGPRREssRPVKPP
S326-p	LGPRREssRPVKPPK
K334	RPVKPPKKDVPDSQQ
D338	PPKKDVPDSQQHPGP
K347	QQHPGPEKSSKISEQ
K363	KCCSGILKEMFAKKH
K368	ILKEMFAKKHAAYAW
K369	LKEMFAKKHAAYAWP
K405	PMDMSTIKSKLESRE
K407	DMSTIKSKLESREYR
S470	EEPVVTVSsPAVPPP
S471-p	EPVVTVSsPAVPPPT
S485	TKVVAPPSSSDSSSD
S489	APPSSSDSSSDSSSD
S493	SSDSSSDSSSDSDSS
S495	DSSSDSSSDSDSSTD
S499	DSSSDSDSSTDDSEE
S500	SSSDSDSSTDDSEEE
S504	SDSSTDDSEEERAQR
S579	KKTKKNNSSNSNVSK
S580	KTKKNNSSNSNVSKK
S582	KKNNSSNSNVSKKEP
S585	NSSNSNVSKKEPVPT
T599-p	TKTKPPPtYEsEEED
Y600	KTKPPPtYEsEEEDK
S602-p	KPPPtYEsEEEDKCK
S620	YEEKRQLSLDINKLP
S706	FSSSESESTSESSSS
S715-p	SESSSSDsEDsETEM
S718-p	SSSDsEDsETEMAPK
K727	TEMAPKSKKKGHTGR
K728	EMAPKSKKKGHTGRD
S860	LNQHAVVSPPALHNA
T944	LQKVQPPTPLLPSVK
S1048-p	QVIQHHPsPRHHKSD
Y1057	RHHKSDPYSAGHLRE
S1067-p	GHLREAPsPLMIHsP
S1073-p	PsPLMIHsPQMPQFQ
T1083-p	MPQFQSLtHQsPPQQ
S1086-p	FQSLtHQsPPQQNVQ
K1147	PQPLVVVKEEKIHsP
K1150	LVVVKEEKIHsPIIR
S1153-p	VKEEKIHsPIIRSEP
S1162	IIRSEPFSTSLRPEP
S1237-p	LKIKNMGsWASLVQK
T1247-p	SLVQKHPtTPSSTAK
T1248	LVQKHPtTPSSTAKS
S1251	KHPtTPSSTAKSSSD

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