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Protein Page:
BRD4 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
BRD4 an atypical protein kinase of the Brd family. Contains a double bromodomain. Binds to chromatin and regulates cell cycle progression at multiple stages. Associates with chromosomes during mitosis. The cognate gene is the chromosome 19 target of translocation t(15;19)(q13;p13.1), resulting in the BRD4-NUT oncogene identified in a lethal carcinoma of young people. Binds to papillomavirus E2 protein and is required for its transforming ability. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, atypical; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; ATYPICAL group; BRD family
Cellular Component: condensed nuclear chromosome; cytoplasm; nuclear chromatin; nucleolus; chromosome; nucleus
Molecular Function: protein binding; DNA binding; p53 binding; chromatin binding
Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; transcription, DNA-dependent; protein amino acid phosphorylation; inner cell mass cell proliferation; chromosome segregation; G1/S-specific transcription in mitotic cell cycle; chromatin remodeling; positive regulation of RNA elongation from RNA polymerase II promoter; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of DNA binding
Reference #:  O60885 (UniProtKB)
Alt. Names/Synonyms: BRD4; bromodomain containing 4; bromodomain-containing 4; Bromodomain-containing protein 4; CAP; chromosome-associated protein; HUNK1; HUNKI; MCAP; Protein HUNK1
Gene Symbols: BRD4
Molecular weight: 152,219 Da
Basal Isoelectric point: 9.23  Predict pI for various phosphorylation states
CST Pathways:  Crosstalk between PTMs
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BRD4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 K99 LNLPDYYKIIKTPMD
0 8 S324-p KLGQRREssRPVKPP
0 4 S325-p LGQRREssRPVKPPK
0 1 D337-ca PPKKDVPdSQQHPAP
0 1 K362-a KCCSGILkEMFAkkH
0 1 K367-a ILkEMFAkkHAAYAW
0 1 K368-a LkEMFAkkHAAYAWP
0 1 S469-p EEPVVAVssPAVPPP
0 11 S470-p EPVVAVssPAVPPPT
1 0 S484-p TKVVAPPsSSDsSSD
1 0 S488-p APPsSSDsSSDsSsD
1 0 S492-p SSDsSSDsSsDSDss
1 0 S494-p DsSSDsSsDSDssTD
1 0 S498-p DsSsDSDssTDDsEE
1 0 S499-p sSsDSDssTDDsEEE
1 0 S503-p SDssTDDsEEERAQR
0 2 S581-p KKNNSSNsNVSKKEP
0 2 T598-p MKSKPPPtyEsEEED
0 2 Y599-p KSKPPPtyEsEEEDK
0 14 S601-p KPPPtyEsEEEDKCK
0 2 K726-a TEMAPKSkkKGHPGR
0 2 K727-a EMAPKSkkKGHPGRE
0 1 S858-p LNQHAVVsPPALHNA
0 2 T942-p LQKVQPPtPLLPSVK
0 4 S1045-p QVIQHHHsPRHHKSD
0 8 S1064-p GHLREAPsPLMIHsP
0 6 S1070-p PsPLMIHsPQMSQFQ
0 2 T1080-p MSQFQSLtHQsPPQQ
0 3 S1083-p FQSLtHQsPPQQNVQ
0 6 K1111-a PQPLVVVkEEkIHsP
0 2 K1114-a LVVVkEEkIHsPIIR
0 29 S1117-p VkEEkIHsPIIRSEP
0 3 S1126-p IIRSEPFsPSLRPEP
0 8 S1201-p LKIKNMGsWAsLVQK
0 1 S1204-p KNMGsWAsLVQKHPT
0 1 T1211 sLVQKHPTtPSsTAK
0 2 T1212-p LVQKHPTtPSsTAKS
0 1 S1215-gl KHPTtPSsTAKSSSD
0 1 S1319 AQSSQPQSMLDQQRE
  BRD4 iso2  
K99 LNLPDYYKIIKTPMD
S324 KLGQRRESSRPVKPP
S325 LGQRRESSRPVKPPK
D337 PPKKDVPDSQQHPAP
K362 KCCSGILKEMFAKKH
K367 ILKEMFAKKHAAYAW
K368 LKEMFAKKHAAYAWP
S469 EEPVVAVSSPAVPPP
S470 EPVVAVSSPAVPPPT
S484 TKVVAPPSSSDSSSD
S488 APPSSSDSSSDSSSD
S492 SSDSSSDSSSDSDSS
S494 DSSSDSSSDSDSSTD
S498 DSSSDSDSSTDDSEE
S499 SSSDSDSSTDDSEEE
S503 SDSSTDDSEEERAQR
S581 KKNNSSNSNVSKKEP
T598 MKSKPPPTYESEEED
Y599 KSKPPPTYESEEEDK
S601 KPPPTYESEEEDKCK
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  BRD4 iso3  
K99 LNLPDYYKIIKTPMD
S324 KLGQRRESSRPVKPP
S325 LGQRRESSRPVKPPK
D337 PPKKDVPDSQQHPAP
K362 KCCSGILKEMFAKKH
K367 ILKEMFAKKHAAYAW
K368 LKEMFAKKHAAYAWP
S469 EEPVVAVSSPAVPPP
S470 EPVVAVSSPAVPPPT
S484 TKVVAPPSSSDSSSD
S488 APPSSSDSSSDSSSD
S492 SSDSSSDSSSDSDSS
S494 DSSSDSSSDSDSSTD
S498 DSSSDSDSSTDDSEE
S499 SSSDSDSSTDDSEEE
S503 SDSSTDDSEEERAQR
S581 KKNNSSNSNVSKKEP
T598 MKSKPPPTYESEEED
Y599 KSKPPPTYESEEEDK
S601 KPPPTYESEEEDKCK
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
K99-u LNLPDYYkIIKTPMD
S325-p KLGPRREssRPVKPP
S326-p LGPRREssRPVKPPK
D338 PPKKDVPDSQQHPGP
K363 KCCSGILKEMFAKKH
K368 ILKEMFAKKHAAYAW
K369 LKEMFAKKHAAYAWP
S470 EEPVVTVSsPAVPPP
S471-p EPVVTVSsPAVPPPT
S485 TKVVAPPSSSDSSSD
S489 APPSSSDSSSDSSSD
S493 SSDSSSDSSSDSDSS
S495 DSSSDSSSDSDSSTD
S499 DSSSDSDSSTDDSEE
S500 SSSDSDSSTDDSEEE
S504 SDSSTDDSEEERAQR
S582 KKNNSSNSNVSKKEP
T599-p TKTKPPPtYEsEEED
Y600 KTKPPPtYEsEEEDK
S602-p KPPPtYEsEEEDKCK
K727 TEMAPKSKKKGHTGR
K728 EMAPKSKKKGHTGRD
S860 LNQHAVVSPPALHNA
T944 LQKVQPPTPLLPSVK
S1048-p QVIQHHPsPRHHKSD
S1067-p GHLREAPsPLMIHsP
S1073-p PsPLMIHsPQMPQFQ
T1083-p MPQFQSLtHQsPPQQ
S1086-p FQSLtHQsPPQQNVQ
K1147 PQPLVVVKEEKIHsP
K1150 LVVVKEEKIHsPIIR
S1153-p VKEEKIHsPIIRSEP
S1162 IIRSEPFSTSLRPEP
S1237-p LKIKNMGsWASLVQK
S1240 KNMGsWASLVQKHPt
T1247-p SLVQKHPtTPSSTAK
T1248 LVQKHPtTPSSTAKS
S1251 KHPtTPSSTAKSSSD
S1357-p AQSSQPQsMLDQQRE
  rat

 
K99 LNLPDYYKIIKTPMD
S325 KLGPRRESSRPVKPP
S326 LGPRRESSRPVKPPK
D338 PPKKDVPDSQQHPGP
K363 KCCSGILKEMFAKKH
K368 ILKEMFAKKHAAYAW
K369 LKEMFAKKHAAYAWP
S470 EEPVVTVSSPAVPPP
S471 EPVVTVSSPAVPPPT
S485 TKVVAPPSSSDSSSD
S489 APPSSSDSSSDSSSD
S493 SSDSSSDSSSDSDSS
S495 DSSSDSSSDSDSSTD
S499 DSSSDSDSSTDDSEE
S500 SSSDSDSSTDDSEEE
S504 SDSSTDDSEEERAQR
S582 KKNNSSNSNVSKKEP
T599 TKTKPPPTYESEEED
Y600 KTKPPPTYESEEEDK
S602 KPPPTYESEEEDKCK
K727 TEMAPKSKKKGHTGR
K728 EMAPKSKKKGHTGRD
S864 LNQHAVVSPPALHNA
T948 LQKVQPPTPLLPSVK
S1052 QVIQHHPSPRHHKSD
S1071 GHLREAPSPLMIHSP
S1077 PSPLMIHSPQMPQFQ
T1087 MPQFQSLTHQSPPQQ
S1090 FQSLTHQSPPQQNVQ
K1151 PQPLVVVKEEKIHsP
K1154 LVVVKEEKIHsPIIR
S1157-p VKEEKIHsPIIRSEP
S1166 IIRSEPFSTSLRPEP
S1241 LKIKNMGSWASLVQK
S1244 KNMGSWASLVQKHPT
T1251 SLVQKHPTTPSSTAK
T1252 LVQKHPTTPSSTAKS
S1255 KHPTTPSSTAKSSSD
S1360 AQSSQPQSMLDQQRE
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