Rab effector protein acting as linker between gamma- adaptin, RAB4A or RAB5A. Involved in endocytic membrane fusion and membrane trafficking of recycling endosomes. Stimulates nucleotide exchange on RAB5A. Can act as a ubiquitin ligase. Interacts with RGS14; the interaction is GTP-dependent. Heterodimer with RABEP1. The heterodimer binds RAB4A and RAB5A that have been activated by GTP-binding. Interacts with RAB21, and with 100-fold lower affinity also with RAB22. Binds TSC2, GGA1, GGA2, GGA3, AP1G1 and AP1G2. Interacts with ubiquitinated EGFR. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: GAPs, Rab; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 7q11.21
Cellular Component: recycling endosome; early endosome; vesicle
Molecular Function: protein binding; DNA binding; zinc ion binding; ubiquitin-protein ligase activity; Rab guanyl-nucleotide exchange factor activity; Rab GTPase binding
Biological Process: negative regulation of protein amino acid phosphorylation; negative regulation of mast cell degranulation; negative regulation of inflammatory response; protein ubiquitination; negative regulation of Ras protein signal transduction; endocytosis; negative regulation of receptor-mediated endocytosis; positive regulation of Rab GTPase activity; protein targeting to membrane; negative regulation of leukocyte migration
SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.