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Protein Page:
A6 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
A6 Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down- regulated. Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Motility/polarity/chemotaxis
Cellular Component: myofibril; perinuclear region of cytoplasm; cytoplasm; intercellular junction; filopodium; actin cytoskeleton
Molecular Function: actin monomer binding; phosphatidylinositol-4,5-bisphosphate binding; protein-tyrosine kinase activity; ATP binding
Biological Process: sequestering of actin monomers; negative regulation of actin filament polymerization; regulation of actin phosphorylation; barbed-end actin filament capping
Reference #:  Q12792 (UniProtKB)
Alt. Names/Synonyms: A6; A6 protein tyrosine kinase; MGC23788; MGC41876; Protein A6; Protein tyrosine kinase 9; PTK9; PTK9 protein tyrosine kinase 9; TWF1; twinfilin, actin-binding protein, homolog 1 (Drosophila); Twinfilin-1
Gene Symbols: TWF1
Molecular weight: 40,283 Da
Basal Isoelectric point: 6.48  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

A6

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y134-p EDVSLHGyKKYLLSQ
0 10 S143-p KYLLSQSsPAPLTAA
0 1 K192 EAFQALEKLNNRQLN
0 140 Y309-p ELTADFLyEEVHPKQ
0 2 K325-a AHKQSFAkPkGPAGK
0 2 K327-a KQSFAkPkGPAGKRG
0 16 T349-p PAETEATtD______
  mouse

 
Y134 EDVSLHGYKKYLLSQ
S143-p KYLLSQSsPAPLTAA
K192-u DAFQALEkLSKKQLN
Y309-p ELTADFLyDEVHPKQ
K325 AHKQSFAKPKGPAGK
K327 KQSFAKPKGPAGKRG
T349-p PAEAEATtD______
  rat

 
Y134 EDVSLHGYRKYLLSQ
S143-p KYLLSQSsPAPLTAA
K192 DAFQALEKLSKRQLN
Y309-p ELTADFLyDEVHPKQ
K325 AHKQSFAKPKGPAGK
K327 KQSFAKPKGPAGKRG
T349 PAEAEATTD______
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