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Protein Page:
TOP2A (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
TOP2A a DNA topoisomerase. Controls of topological states of DNA by transient breakage and rejoining of DNA. Makes double-strand breaks. Four splice variant isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Isomerase; EC 5.99.1.3
Cellular Component: centriole; nucleoplasm; nuclear chromosome; protein complex; viral integration complex; DNA topoisomerase complex (ATP-hydrolyzing); nucleolus; synaptonemal complex; nucleus
Molecular Function: protein C-terminus binding; DNA topoisomerase (ATP-hydrolyzing) activity; DNA-dependent ATPase activity; ubiquitin binding; protein homodimerization activity; histone deacetylase binding; magnesium ion binding; drug binding; protein binding; enzyme binding; protein kinase C binding; DNA binding; protein heterodimerization activity; chromatin binding; DNA bending activity; ATP binding
Biological Process: ATP catabolic process; mitotic recombination; phosphoinositide-mediated signaling; positive regulation of viral genome replication; DNA topological change; positive regulation of apoptosis; sister chromatid segregation; apoptotic chromosome condensation; DNA repair; embryonic cleavage; positive regulation of retroviral genome replication; DNA ligation; chromosome segregation; resolution of meiotic joint molecules as recombinants; positive regulation of transcription from RNA polymerase II promoter; mitotic cell cycle; DNA-dependent DNA replication; DNA replication; response to DNA damage stimulus
Reference #:  P11388 (UniProtKB)
Alt. Names/Synonyms: DNA topoisomerase 2-alpha; DNA topoisomerase II, 170 kD; DNA topoisomerase II, alpha isozyme; TOP2; TOP2A; topoisomerase (DNA) II alpha 170kDa; TP2A
Gene Symbols: TOP2A
Molecular weight: 174,385 Da
Basal Isoelectric point: 8.82  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

TOP2A

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB  |  ENZYME  |  Phospho3D  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
apoptosis, induced: S1337‑p, S1525‑p
cell cycle regulation: S1337‑p, S1525‑p
chromatin organization, altered: S1525‑p
enzymatic activity, induced: S1106‑p, S1337‑p, S1525‑p
intracellular localization: S1213‑p, S1337‑p, T1343‑p, S1525‑p
molecular association, regulation: S1106‑p, S1361‑p, S1365‑p, S1525‑p
phosphorylation: S1365‑p
protein degradation: S1361‑p, S1365‑p
ubiquitination: S1361‑p, S1365‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 9 S4-p ____MEVsPLQPVNE
0 8 S29-p EDAKKRLsVERIYQK
0 1 K123-u ISIWNNGkGIPVVEH
0 18 K131-u GIPVVEHkVEKMYVP
0 24 K156-u SNYDDDEkkVTGGRN
0 10 K157-u NYDDDEkkVTGGRNG
0 2 S230-p LSKFKMQsLDkDIVA
0 1 K233-u FKMQsLDkDIVALMV
0 1 K278-u VDMYLKDkLDEtGNs
0 2 T282-p LKDkLDEtGNsLkVI
0 1 S285-p kLDEtGNsLkVIHEQ
0 1 K287-u DEtGNsLkVIHEQVN
0 1 K321-u VNSIATSkGGRHVDy
0 6 Y328-p kGGRHVDyVADQIVT
0 3 K386-u ENMTLQPkSFGSTCQ
0 1 K418-u ILNWVKFkAQVQLNK
0 2 K480-u LGVVGRDkYGVFPLR
0 3 K499-u NVREASHkQIMENAE
0 2 K520-u IVGLQYKkNYEDEDS
0 3 K529-u YEDEDSLkTLRYGKI
0 3 K579-u EFITPIVkVSKNkQE
0 1 K584-u IVkVSKNkQEMAFYS
0 1 T602 FEEWKSSTPNHKKWK
0 2 K611 NHKKWKVKYYkGLGT
0 1 K614-u KWKVKYYkGLGTSTS
0 2 K622-u GLGTSTSkEAKEYFA
0 1 K639-u KRHRIQFkYSGPEDD
0 1 K655-u AISLAFSkkQIDDRK
0 3 K656-u ISLAFSkkQIDDRKE
0 1 K676-u MEDRRQRkLLGLPED
0 1 K723-u PSMVDGLkPGQRKVL
0 6 Y805-p KDSASPRyIFTMLSS
0 1 K827-u PKDDHTLkFLYDDNQ
0 1 K893-u LPMLPSYkNFKGTIE
0 1 K936-u RTWTQTYkEQVLEPM
0 2 K949-u PMLNGTEkTPPLITD
0 1 K1011-u DHVGCLKkYDTVLDI
0 1 K1028-u DFFELRLkYYGLRKE
0 1 K1062-u ILEKIDGkIIIENKP
0 1 K1075-u KPKKELIkVLIQRGY
2 18 S1106-p DEEENEEsDNEKEtE
0 2 T1112-p EsDNEKEtEKSDSVt
0 1 T1119-p tEKSDSVtDSGPTFN
0 1 K1159-u EQELDTLkRKSPSDL
0 1 K1196-u EQVGLPGkGGKAKGK
0 1 K1204-u GGKAKGKktQMAEVL
0 4 T1205-p GKAKGKktQMAEVLP
4 36 S1213-p QMAEVLPsPRGQRVI
0 1 K1228-s PRITIEMkAEAEKKN
0 1 K1240-s KKNKKKIkNENtEGs
0 4 T1244-p KKIkNENtEGsPQED
1 31 S1247-p kNENtEGsPQEDGVE
0 3 G1257 EDGVELEGLkQRLEK
0 1 K1259-u GVELEGLkQRLEKKQ
0 7 S1295-p GKKRNPWsDsEsDRs
0 4 S1297-p KRNPWsDsEsDRssD
0 2 S1299-p NPWsDsEsDRssDES
0 4 S1302-p sDsEsDRssDESNFD
0 4 S1303-p DsEsDRssDESNFDV
0 1 S1306 sDRssDESNFDVPPR
0 3 T1324-p PRRAATKtKFtMDLD
0 6 T1327-p AATKtKFtMDLDsDE
0 32 S1332-p KFtMDLDsDEDFsDF
1 35 S1337-p LDsDEDFsDFDEKtD
4 20 T1343-p FsDFDEKtDDEDFVP
0 9 S1351-p DDEDFVPsDAsPPKT
1 21 S1354-p DFVPsDAsPPKTKTs
2 0 S1361-p sPPKTKTsPKLsNKE
1 1 S1365-p TKTsPKLsNKELKPQ
0 1 - gap
0 28 S1374-p KELKPQKsVVsDLEA
0 1 - gap
2 47 S1377-p KPQKsVVsDLEADDV
0 2 - gap
0 6 S1387-p EADDVKGsVPLsssP
0 11 S1391-p VKGsVPLsssPPAtH
0 7 S1392-p KGsVPLsssPPAtHF
1 21 S1393-p GsVPLsssPPAtHFP
0 5 T1397-p LsssPPAtHFPDEtE
0 2 T1403-p AtHFPDEtEITNPVP
0 2 T1430-p SQSSTSTtGAKKRAA
0 1 R1443 AAPKGTKRDPALNsG
0 4 S1449-p KRDPALNsGVsQKPD
0 3 S1452-p PALNsGVsQKPDPAK
1 117 S1469-p NRRKRKPstsDDsDs
0 78 T1470-p RRKRKPstsDDsDsN
0 88 S1471-p RKRKPstsDDsDsNF
0 7 D1473 RKPstsDDsDsNFEK
0 89 S1474-p KPstsDDsDsNFEKI
0 36 S1476-p stsDDsDsNFEKIVS
0 3 S1491-p KAVTSKKsKGEsDDF
0 6 S1495-p SKKsKGEsDDFHMDF
0 9 S1504-p DFHMDFDsAVAPRAK
0 3 Y1521-p RAKKPIKyLEEsDED
4 25 S1525-p PIKyLEEsDEDDLF_
13072 : Phospho-Topoisomerase II┬┐ (Ser1469) (D4F5) Rabbit mAb
  TOP2A iso2  
S4 ____MEVSPLQPVNE
S29 EDAKKRLSVERIYQK
K123 ISIWNNGKGIPVVEH
K131 GIPVVEHKVEKMYVP
K156 SNYDDDEKKVTGGRN
K157 NYDDDEKKVTGGRNG
S230 LSKFKMQSLDKDIVA
K233 FKMQSLDKDIVALMV
K278 VDMYLKDKLDETGNS
T282 LKDKLDETGNSLKVI
S285 KLDETGNSLKVIHEQ
K287 DETGNSLKVIHEQVN
K321 VNSIATSKGGRHVDY
Y328 KGGRHVDYVADQIVT
K386 ENMTLQPKSFGSTCQ
K444 ILNWVKFKAQVQLNK
K506 LGVVGRDKYGVFPLR
K525 NVREASHKQIMENAE
K546 IVGLQYKKNYEDEDS
K555 YEDEDSLKTLRYGKI
K605 EFITPIVKVSKNKQE
K610 IVKVSKNKQEMAFYS
T628 FEEWKSSTPNHKKWK
K637 NHKKWKVKYYKGLGT
K640 KWKVKYYKGLGTSTS
K648 GLGTSTSKEAKEYFA
K665 KRHRIQFKYSGPEDD
K681 AISLAFSKKQIDDRK
K682 ISLAFSKKQIDDRKE
K702 MEDRRQRKLLGLPED
K749 PSMVDGLKPGQRKVL
Y831 KDSASPRYIFTMLSS
K853 PKDDHTLKFLYDDNQ
K919 LPMLPSYKNFKGTIE
K962 RTWTQTYKEQVLEPM
K975 PMLNGTEKTPPLITD
K1037 DHVGCLKKYDTVLDI
K1054 DFFELRLKYYGLRKE
K1088 ILEKIDGKIIIENKP
K1101 KPKKELIKVLIQRGY
S1132 DEEENEESDNEKETE
T1138 ESDNEKETEKSDSVT
T1145 TEKSDSVTDSGPTFN
K1185 EQELDTLKRKSPSDL
K1222 EQVGLPGKGGKAKGK
K1230 GGKAKGKKTQMAEVL
T1231 GKAKGKKTQMAEVLP
S1239-p QMAEVLPsPRGQRVI
K1254 PRITIEMKAEAEKKN
K1266 KKNKKKIKNENTEGS
T1270 KKIKNENTEGSPQED
S1273 KNENTEGSPQEDGVE
G1283 EDGVELEGLKQRLEK
K1285 GVELEGLKQRLEKKQ
S1321 GKKRNPWSDSESDRS
S1323 KRNPWSDSESDRSSD
S1325 NPWSDSESDRSSDES
S1328 SDSESDRSSDESNFD
S1329 DSESDRSSDESNFDV
S1332 SDRSSDESNFDVPPR
T1350 PRRAATKTKFTMDLD
T1353 AATKTKFTMDLDSDE
S1358 KFTMDLDSDEDFSDF
S1363 LDSDEDFSDFDEKTD
T1369 FSDFDEKTDDEDFVP
S1377 DDEDFVPSDASPPKT
S1380 DFVPSDASPPKTKTS
S1387 SPPKTKTSPKLSNKE
S1391 TKTSPKLSNKELKPQ
- gap
S1400-p KELKPQKsVVSDLEA
- gap
S1403 KPQKsVVSDLEADDV
- gap
S1413 EADDVKGSVPLSSsP
S1417 VKGSVPLSSsPPATH
S1418 KGSVPLSSsPPATHF
S1419-p GSVPLSSsPPATHFP
T1423 LSSsPPATHFPDETE
T1429 ATHFPDETEITNPVP
T1456 SQSSTSTTGAKKRAA
R1469 AAPKGTKRDPALNSG
S1475 KRDPALNSGVSQKPD
S1478 PALNSGVSQKPDPAK
S1495-p NRRKRKPstsDDsDs
T1496-p RRKRKPstsDDsDsN
S1497-p RKRKPstsDDsDsNF
D1499 RKPstsDDsDsNFEK
S1500-p KPstsDDsDsNFEKI
S1502-p stsDDsDsNFEKIVS
S1517 KAVTSKKSKGESDDF
S1521 SKKSKGESDDFHMDF
S1530 DFHMDFDSAVAPRAK
Y1547 RAKKPIKYLEESDED
S1551 PIKYLEESDEDDLF_
  mouse

 
S4-p ____MELsPLQPVNE
S28-p EDGKKRLsIERIYQK
K122 ISIWNNGKGIPVVEH
K130 GIPVVEHKVEKIYVP
K155-u SNYDDDEkkVTGGRN
K156-u NYDDDEkkVTGGRNG
S229-p LSKFKMQsLDKDIVA
K232 FKMQsLDKDIVALMV
K277 VDLYLKDKVDETGNS
T281 LKDKVDETGNSLKVI
S284 KVDETGNSLKVIHEQ
K286 DETGNSLKVIHEQVN
K320 VNSIATSKGGRHVDy
Y327-p KGGRHVDyVADQIVS
K385 ENMTLQAKSFGSTCQ
K417 ILNWVKFKAQIQLNK
K479 LGVVGRDKYGVFPLR
K498 NVREASHKQIMENAE
K519 IVGLQYKKNYEDEDS
K528 YEDEDSLKTLRYGKI
K578 EFITPIVKVSKNKQE
K583 IVKVSKNKQEIAFYS
T601-p FEEWKSStPNHKKWK
K610-a NHKKWKVkYYKGLGT
K613 KWKVkYYKGLGTSTS
K621-u GLGTSTSkEAKEYFA
K638 KRHRIQFKYSGPEDD
K654 AISLAFSKKQVDDRK
K655 ISLAFSKKQVDDRKE
K675 MEDRRQRKLLGLPED
K722 PSMVDGLKPGQRKVL
Y804 KDSASPRYIFTMLSP
R826 PKDDHTLRFLYDDNQ
K892 LPMLPSYKNFKGTIE
K935 RTWTQTYKEQVLEPM
K948 PMLNGTEKTPSLITD
K1010 DHVGCLKKYDTVLDI
K1027 DFFELRLKYYGLRKE
K1061 ILEKIDGKIVIENKP
K1074 KPKKELIKVLIQRGY
S1105-p DEEENEEsDTETSTS
T1109 NEEsDTETSTSDSAA
A1116 TSTSDSAAEAGPTFN
K1156 EQELNTLKQKSPSDL
K1193 EQVGLPGKAGKAKGK
K1201 AGKAKGKKAQMCADV
- gap
S1211-p MCADVLPsPRGKRVI
K1226 PQVTVEMKAEAEKKI
K1238 KKIRKKIKSENVEGt
- gap
T1245-p KSENVEGtPAEDGAE
S1255-p EDGAEPGsLRQRIEK
R1257 GAEPGsLRQRIEKKQ
S1291-p GRKKNPWsDsEsDVs
S1293-p KKNPWsDsEsDVssN
S1295-p NPWsDsEsDVssNEs
S1298-p sDsEsDVssNEsNVD
S1299-p DsEsDVssNEsNVDV
S1302-p sDVssNEsNVDVPPR
- gap
T1323-p AAAKAKFtVDLDsDE
S1328-p KFtVDLDsDEDFsGL
S1333-p LDsDEDFsGLDEKDE
- gap
- gap
T1350-p DFLPLDAtPPKAKIP
- gap
T1361 AKIPPKNTKKALKtQ
T1367-p NTKKALKtQGssMsV
S1370-p KALKtQGssMsVVDL
S1371-p ALKtQGssMsVVDLE
S1373-p KtQGssMsVVDLEsD
S1379-p MsVVDLEsDVKDsVP
S1384-p LEsDVKDsVPAsPGV
S1388-p VKDsVPAsPGVPAAD
- gap
- gap
- gap
T1400 AADFPAETEQSKPSK
- gap
S1439-p AAPKGTKsDSALSAR
- gap
S1448 SALSARVSEKPAPAK
S1465-p NSRKRKPsssDssDs
S1466-p SRKRKPsssDssDsD
S1467-p RKRKPsssDssDsDF
S1469-p RKPsssDssDsDFER
S1470-p KPsssDssDsDFERA
S1472-p sssDssDsDFERAIS
- gap
- gap
- gap
Y1517 RARKPIKYLEEsDDD
S1521-p PIKYLEEsDDDDDLF
  rat

 
S4 ____MELSPLQPVNE
S28-p EDGKKRLsVERIYQK
K121 ISIWNNGKGIPVVEH
K129 GIPVVEHKVEKMYVP
K154 SNYDDDEKKVTGGRN
K155 NYDDDEKKVTGGRNG
S228 LSKFKMQSLDKDIVA
K231 FKMQSLDKDIVALMV
K276 VDMYLKDKVDETGNA
T280 LKDKVDETGNALKVV
- gap
K285 DETGNALKVVHEQVN
K319 VNSIATSKGGRHVDY
Y326 KGGRHVDYVADQIVS
K384 ENMTLQAKSFGSTCQ
K416 ILNWVKFKAQIQLNK
K478 LGVVGRDKYGVFPLR
K497 NVREASHKQIMENAE
K518 IVGLQYKKNYEDEDS
K527 YEDEDSLKTLRYGKI
K577 EFITPIVKVSKNKQE
K582 IVKVSKNKQEIAFYS
N600 FEEWKSTNPNHKKWK
K609 NHKKWKVKYYKGLGT
K612 KWKVKYYKGLGTSTS
K620 GLGTSTSKEAKEYFA
K637 KRHRIQFKYSGPEDD
K653 AISLAFSKKQVDDRK
K654 ISLAFSKKQVDDRKE
K674 MEDRRQRKLLGLPED
K721 PSMVDGLKPGQRKVL
Y803 KDSASPRYIFTMLSP
R825 SKDDHTLRFLYDDNQ
K891 LPMLPSYKNYKGTIE
K934 RTWTQTYKEQVLEPM
K947 PMLNGTEKTPPLITD
K1009 DHVGCLKKYDTVLDI
K1026 DFFELRLKYYGLRKE
K1060 ILEKIDGKIVIENKP
K1073 KPKKELIKVLIQRGY
N1104 EEEENEENEESESES
S1109 EENEESESESTSPAA
A1116 SESTSPAAESGPTFN
K1156 EQELNTLKKKTPSDL
K1193 EQVGLPGKGVKAKGK
K1201 GVKAKGKKAQISEVL
- gap
S1210-p QISEVLPsPVGKRVI
R1225 PQVTMEMRAEAEKKI
K1237 KKIRRKIKSENVEGt
- gap
T1244-p KSENVEGtPAEDGAE
- gap
R1255 DGAEPGLRQRLEKRQ
S1291 AQKQNPWSDSESDMS
S1293 KQNPWSDSESDMSSN
S1295 NPWSDSESDMSSNES
S1298 SDSESDMSSNESNFD
S1299 DSESDMSSNESNFDV
S1302 SDMSSNESNFDVPPR
- gap
T1323 AATKAKFTADLDsDD
S1328-p KFTADLDsDDDFsGL
S1333-p LDsDDDFsGLDEKDE
- gap
- gap
T1350-p DFFPLDDtPPKTKMP
- gap
T1361 TKMPPKNTKKALKPQ
- gap
S1370 KALKPQKSSTsVDLE
S1371 ALKPQKSSTsVDLEs
S1373-p KPQKSSTsVDLEsDG
S1378-p STsVDLEsDGKDsVP
S1383-p LEsDGKDsVPAsPGA
S1387-p GKDsVPAsPGASAAD
- gap
- gap
- gap
T1399 AADVPAETEPSKPSS
- gap
S1439 AVPKETKSDSALNAH
- gap
S1448 SALNAHVSKKPAPAK
S1465 NSRKRMPSSSDSSDS
S1466 SRKRMPSSSDSSDSE
S1467 RKRMPSSSDSSDSEF
S1469 RMPSSSDSSDSEFEK
S1470 MPSSSDSSDSEFEKA
S1472 SSSDSSDSEFEKAIS
- gap
- gap
- gap
Y1517 RARKPIKYLEESDDD
S1521 PIKYLEESDDDLF__
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