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Protein Page:
nestin (rat)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
nestin an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments. Note: This description may include information from UniProtKB.
Protein type: Cytoskeletal protein
Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament; intracellular
Molecular Function: intermediate filament binding; structural molecule activity; CCR5 chemokine receptor binding
Biological Process: response to drug; response to nutrient levels; positive regulation of intermediate filament depolymerization; neuron differentiation; nervous system development; negative regulation of catalytic activity; negative regulation of neuron apoptosis; cell projection morphogenesis; response to ionizing radiation; brain development; G2/M transition of mitotic cell cycle; negative regulation of protein binding; embryonic camera-type eye development
Reference #:  P21263 (UniProtKB)
Alt. Names/Synonyms: Nes; NEST; Nestin
Gene Symbols: Nes
Molecular weight: 208,797 Da
Basal Isoelectric point: 4.3  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

nestin

Protein Structure Not Found.


STRING  |  BioGPS  |  Scansite  |  Pfam  |  Phospho.ELM  |  Source  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       rat

► Hide Isoforms
 
0 1 Y22 LNRRLEAYLTRVKTL
0 1 K27 EAYLTRVKTLEEQNQ
0 6 K74 VDQRWREKLEAEVQR
0 13 T105 VRLARERTVQEAACS
0 7 K120 RRALEAEKNARGWLS
0 1 A158 LNAQAACAPRRPPAP
0 2 S169 PPAPPHGSPVRAPEV
0 7 K260 DRLQATDKFQLAVEA
0 6 K272 VEALEQEKQGLQSQI
0 1 S277 QEKQGLQSQIAQILE
0 1 S295 QLAHLKMSLSLEVAT
0 3 S312 TLLEAENSRLQTPGR
2 5 T316 AENSRLQTPGRGSQA
0 4 S321 LQTPGRGSQASLGFL
0 10 Q322 QTPGRGSQASLGFLD
0 1 - gap
0 1 S324 PGRGSQASLGFLDPK
0 15 - gap
0 3 K331 SLGFLDPKLKPNFLG
0 21 - gap
0 2 S347 PEDQYLGSVLPALSP
0 5 S353 GSVLPALSPTSFPSP
0 3 S356 LPALSPTSFPSPLPN
0 5 S359 LSPTSFPSPLPNTLE
0 1 T367 PLPNTLETPVTAFLK
0 1 T383 QEFLQARTPTLASTP
0 3 T385 FLQARTPTLASTPIP
0 1 S388 ARTPTLASTPIPPIS
0 3 T389 RTPTLASTPIPPISE
0 1 - gap
0 7 - gap
0 1 S413 RAQEVPLSLLQTQAP
0 1 - gap
0 3 - gap
0 13 - gap
0 1 - gap
0 2 - gap
0 4 S475 LEAKDGESSGSRVSS
0 1 S476 EAKDGESSGSRVSSI
0 1 S478 KDGESSGSRVSSIFQ
0 1 S481 ESSGSRVSSIFQEDE
0 4 S482 SSGSRVSSIFQEDEG
0 7 K497 QIWELVEKEADIEVK
0 1 K504 KEADIEVKVENSSAQ
0 1 S508 IEVKVENSSAQKTQE
0 1 - gap
0 1 S526 DTEETQDSQGPLQKE
0 1 T534 QGPLQKETLKALGEE
0 9 - gap
0 1 S562 GKENCNSSTEGHLGT
0 1 - gap
0 1 - gap
0 1 K582 KEKQIPLKSLEEKNV
0 1 K587 PLKSLEEKNVESEKT
0 3 S591 LEEKNVESEKTLENG
0 1 K593 EKNVESEKTLENGVP
0 1 T594 KNVESEKTLENGVPV
0 4 S620-p TEDQELMsPKGTLKR
0 14 S685 EDQSFPRSPEEEDQE
0 1 - gap
0 2 R695 EEDQEACRPLQKENQ
0 2 - gap
0 4 S726 IEKESQESLRsPEEE
0 5 S729-p ESQESLRsPEEEDQE
0 3 S814 IEKESQESLRsPEEE
0 4 S817-p ESQESLRsPEEEDQE
0 2 S855 ERLIEKESQESLKSP
0 2 S858 IEKESQESLKSPEEN
0 9 S861 ESQESLKSPEENQRI
0 15 S903 RNQRPLRSLEVEEEE
0 2 - gap
0 1 - gap
0 1 S921 VKPLEKVSQDSLGsL
0 6 S924 LEKVSQDSLGsLAEE
0 3 S927-p VSQDSLGsLAEENVQ
0 4 P935 LAEENVQPLRYLEED
0 2 S960-p KTHKSLGsLEDRNGD
0 2 S999 VNHLEKESQEFSRSS
0 4 S1005 ESQEFSRSSEEEERV
0 5 S1016 EERVMERSLEGENHE
0 5 S1049-p ESQDSGKsLEDESQE
0 10 - gap
0 13 S1071 ENAESLRSLAGQDQE
0 1 - gap
0 1 - gap
0 3 - gap
0 3 K1144 EKGIETVKSLETEII
0 2 S1145 KGIETVKSLETEIIE
0 2 T1148 ETVKSLETEIIEPLE
0 5 - gap
0 1 - gap
0 3 T1156 EIIEPLETAEEDLER
0 3 R1163 TAEEDLERRKsIDTQ
0 18 S1166-p EDLERRKsIDTQEPL
0 9 T1169 ERRKsIDTQEPLWST
0 3 S1175 DTQEPLWSTEVARET
0 3 - gap
0 3 T1205 DENRETLTSLEKESQ
0 1 S1216 KESQELSSLGKWNVE
0 7 - gap
0 1 - gap
0 2 S1229 VETRVEDSQQCLQVE
0 6 - gap
0 8 H1244 EGLQEEQHQESLREV
0 2 S1247 QEEQHQESLREVKQE
0 10 - gap
0 1 S1258 VKQELPSSGNQQRWE
0 1 - gap
0 3 - gap
0 2 S1322 GEAWSLGSSEPKEQR
0 1 S1369 AQAGEQDSIEVTLGL
0 2 S1457 ECQGHEESESMEGWE
0 3 S1510 PKLLEPCSPIPILTD
0 1 - under review  
0 1 - gap
0 1 - gap
0 7 S1570 WEEGREESEADDLGE
2 0 T1583 GETLPDSTPLGLYLR
0 4 S1591 PLGLYLRSPAsPKWD
0 6 S1594-p LYLRSPAsPKWDLAG
0 5 - gap
0 4 S1686 CWDQGGESDGFADEE
0 12 S1695 GFADEEESGEEGEEE
0 1 - gap
0 1 S1721 QWWGSGASGGGCKVQ
0 2 - gap
0 2 - gap
0 1 - gap
0 2 - gap
0 1 S1765 VPALEMVSQDSAEPS
0 1 S1768 LEMVSQDSAEPSGSE
0 4 S1772 SQDSAEPSGSEESES
0 3 S1774 DSAEPSGSEESESAS
0 2 S1777 EPSGSEESESASLEG
0 6 - gap
0 2 - gap
0 1 - gap
0 10 S1866-p LKVPLVAsPVHLGPS
0 1 K1877 LGPSQPLKFTLSGVD
0 3 S1887 LSGVDGDSWssGED_
0 10 S1889-p GVDGDSWssGED___
0 9 S1890-p VDGDSWssGED____
  nestin iso2  
Y22 LNRRLEAYLTRVKTL
K27 EAYLTRVKTLEEQNQ
K74 VDQRWREKLEAEVQR
T105 VRLARERTVQEAACS
K120 RRALEAEKNARGWLS
A158 LNAQAACAPRRPPAP
S169 PPAPPHGSPVRAPEV
K260 DRLQATDKFQLAVEA
K272 VEALEQEKQGLQSQI
S277 QEKQGLQSQIAQILE
S295 QLAHLKMSLSLEVAT
S312 TLLEAENSRLQtPGR
T316-p AENSRLQtPGRGSQA
S321 LQtPGRGSQASLGFL
Q322 QtPGRGSQASLGFLD
- gap
S324 PGRGSQASLGFLDPK
- gap
K331 SLGFLDPKLKPNFLG
- gap
S347 PEDQYLGSVLPALSP
S353 GSVLPALSPTSFPSP
S356 LPALSPTSFPSPLPN
S359 LSPTSFPSPLPNTLE
T367 PLPNTLETPVTAFLK
T383 QEFLQARTPTLASTP
T385 FLQARTPTLASTPIP
S388 ARTPTLASTPIPPIS
T389 RTPTLASTPIPPISE
- gap
- gap
S413 RAQEVPLSLLQTQAP
- gap
- gap
- gap
- gap
- gap
S475 LEAKDGESSGSRVSS
S476 EAKDGESSGSRVSSI
S478 KDGESSGSRVSSIFQ
S481 ESSGSRVSSIFQEDE
S482 SSGSRVSSIFQEDEG
K497 QIWELVEKEADIEVK
K504 KEADIEVKVENSSAQ
S508 IEVKVENSSAQKTQE
- gap
S526 DTEETQDSQGPLQKE
T534 QGPLQKETLKALGEE
- gap
S562 GKENCNSSTEGHLGT
- gap
- gap
K582 KEKQIPLKSLEEKNV
K587 PLKSLEEKNVESEKT
S591 LEEKNVESEKTLENG
K593 EKNVESEKTLENGVP
T594 KNVESEKTLENGVPV
S620 TEDQELMSPKGTLKR
S685 EDQSFPRSPEEEDQE
- gap
R695 EEDQEACRPLQKENQ
- gap
S726 IEKESQESLRSPEEE
S729 ESQESLRSPEEEDQE
- gap
- gap
S767 ERLIEKESQESLKSP
S770 IEKESQESLKSPEEN
S773 ESQESLKSPEENQRI
S815 RNQRPLRSLEVEEEE
- gap
- gap
S833 VKPLEKVSQDSLGSL
S836 LEKVSQDSLGSLAEE
S839 VSQDSLGSLAEENVQ
P847 LAEENVQPLRYLEED
S872 KTHKSLGSLEDRNGD
S911 VNHLEKESQEFSRSS
S917 ESQEFSRSSEEEERV
S928 EERVMERSLEGENHE
S961 ESQDSGKSLEDESQE
- gap
S983 ENAESLRSLAGQDQE
- gap
- gap
- gap
K1056 EKGIETVKSLETEII
S1057 KGIETVKSLETEIIE
T1060 ETVKSLETEIIEPLE
- gap
- gap
T1068 EIIEPLETAEEDLER
R1075 TAEEDLERRKSIDTQ
S1078 EDLERRKSIDTQEPL
T1081 ERRKSIDTQEPLWST
S1087 DTQEPLWSTEVARET
- gap
T1117 DENRETLTSLEKESQ
S1128 KESQELSSLGKWNVE
- gap
- gap
S1141 VETRVEDSQQCLQVE
- gap
H1156 EGLQEEQHQESLREV
S1159 QEEQHQESLREVKQE
- gap
S1170 VKQELPSSGNQQRWE
- gap
- gap
S1234 GEAWSLGSSEPKEQR
S1281 AQAGEQDSIEVTLGL
S1369 ECQGHEESESMEGWE
S1422 PKLLEPCSPIPILTD
- under review  
- gap
- gap
S1482 WEEGREESEADDLGE
T1495-p GETLPDStPLGLYLR
S1503 PLGLYLRSPASPKWD
S1506 LYLRSPASPKWDLAG
- gap
S1598 CWDQGGESDGFADEE
S1607 GFADEEESGEEGEEE
- gap
S1633 QWWGSGASGGGCKVQ
- gap
- gap
- gap
- gap
S1677 VPALEMVSQDSAEPS
S1680 LEMVSQDSAEPSGSE
S1684 SQDSAEPSGSEESES
S1686 DSAEPSGSEESESAS
S1689 EPSGSEESESASLEG
- gap
- gap
- gap
S1778 LKVPLVASPVHLGPS
K1789 LGPSQPLKFTLSGVD
S1799 LSGVDGDSWSSGED_
S1801 GVDGDSWSSGED___
S1802 VDGDSWSSGED____
  human

 
Y22-p LNRRLEAyLARVkAL
K27-ub EAyLARVkALEEQNE
K74-ub VDQRWREkHAAEVAR
T105 LRLARERTTEEVARN
K120-ub RRAVEAEkCARAWLS
A158 LNAQAACAPRCPAPP
P168 CPAPPRGPPAPAPEV
K259-ub ERLRATEkFQLAVEA
K271-ub VEALEQEkQGLQsQI
S276-p QEkQGLQsQIAQVLE
S294-p QLAHLKMsLSLEVAT
S311-p TLLEAENsRLQtPGG
T315-p AENsRLQtPGGGskt
S320-p LQtPGGGsktsLsFQ
K321-ub QtPGGGsktsLsFQD
T322-p tPGGGsktsLsFQDP
S323-p PGGGsktsLsFQDPk
S325-p GGsktsLsFQDPkLE
K330-ub sLsFQDPkLELQFPR
T338-p LELQFPRtPEGRRLG
S346-p PEGRRLGsLLPVLsP
S352-p GsLLPVLsPTsLPsP
S355-p LPVLsPTsLPsPLPA
S358-p LsPTsLPsPLPATLE
T366 PLPATLETPVPAFLK
T382-p QEFLQARtPtLAstP
T384-p FLQARtPtLAstPIP
S387-p ARtPtLAstPIPPtP
T388-p RtPtLAstPIPPtPQ
T393-p AstPIPPtPQAPsPA
S398-p PPtPQAPsPAVDAEI
S413-p RAQDAPLsLLQtQGG
T417-p APLsLLQtQGGRKQA
S459-p QEASTGQsPEDHASL
S471-p ASLAPPLsPDHssLE
S475-p PPLsPDHssLEAKDG
S476-p PLsPDHssLEAKDGE
S484-p LEAKDGEsGGsRVFs
- gap
S487-p KDGEsGGsRVFsICR
- gap
S491-p sGGsRVFsICRGEGE
K507-ub QIWGLVEkETAIEGk
K514-ub kETAIEGkVVSsLQQ
S518-p IEGkVVSsLQQEIWE
K532-ub EEEDLNRkEIQDsQV
S537-p NRkEIQDsQVPLEKE
T545-p QVPLEKEtLKsLGEE
S548-p LEKEtLKsLGEEIQE
- gap
- gap
- gap
K558 EEIQESLKTLENQsH
- gap
S564-p LKTLENQsHEtLERE
- gap
T567-p LENQsHEtLERENQE
- gap
S578-p ENQECPRsLEEDLEt
T585-p sLEEDLEtLksLEKE
K587-ub EEDLEtLksLEKENK
S588-p EDLEtLksLEKENKE
- gap
- gap
- gap
- gap
- gap
- gap
S638-p ENQELMKsLEGNLET
S680-p ENQEPLRsPEVGDEE
S702-p ENQEPLRsLEDENkE
K708-ub RsLEDENkEAFRsLE
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S713-p ENkEAFRsLEKENQE
S746-p ENHKSLRsLEEQDQE
T757-p QDQETLRtLEKETQQ
S768-p ETQQRRRsLGEQDQM
T776-p LGEQDQMtLRPPEKV
S790-p VDLEPLKsLDQEIAR
S814-p LKSLKEEsVEAVksL
K819-ub EEsVEAVksLEtEIL
S820-p EsVEAVksLEtEILE
T823-p EAVksLEtEILEsLk
S828-p LEtEILEsLksAGQE
K830-ub tEILEsLksAGQENL
S831-p EILEsLksAGQENLE
T839-p AGQENLEtLKsPEtQ
S842-p ENLEtLKsPEtQAPL
T845-p EtLKsPEtQAPLWtP
T851-p EtQAPLWtPEEINQG
S891-p LEEENQEsLRsLGAW
- gap
S894-p ENQEsLRsLGAWNLE
S905-p WNLENLRsPEEVDkE
K911-ub RsPEEVDkEsQRNLE
S913-p PEEVDkEsQRNLEEE
K925-ub EEEENLGkGEyQEsL
Y928-p ENLGkGEyQEsLRsL
S931-p GkGEyQEsLRsLEEE
S934-p EyQEsLRsLEEEGQE
S945-p EGQELPQsADVQRWE
D947 QELPQsADVQRWEDT
S965-p DQELAQEsPPGMAGV
S1016-p PGEGHPEsPEPKEQR
S1079 APGGDQASPEVMLGS
S1175-p PREGREEsEAEAPRG
T1224 VLVSPSPTyTPILED
Y1225-p LVSPSPTyTPILEDA
S1260-p AEAGKVEsEQEELGS
S1282-p PQEEGEEsREEsEED
S1286-p GEEsREEsEEDELGE
T1299 GETLPDSTPLGFYLR
S1307 PLGFYLRSPTsPRWD
S1310-p FYLRSPTsPRWDPTG
S1347-p EGLEAPPsEKEEGEE
S1409-p AWDRDGEsDGFADEE
S1418-p GFADEEEsGEEGEED
S1441-p AGRWGPGssVGsLQA
S1442-p GRWGPGssVGsLQAL
S1445-p GPGssVGsLQALsss
S1450-p VGsLQALsssQRGEF
S1451-p GsLQALsssQRGEFL
S1452-p sLQALsssQRGEFLE
S1489-p KTALETEsQDsAEPs
S1492-p LETEsQDsAEPsGsE
S1496-p sQDsAEPsGsEEEsD
S1498-p DsAEPsGsEEEsDPV
S1502-p PsGsEEEsDPVSLER
S1577-p VSEGDRGsPFQEEEG
S1585-p PFQEEEGsALKtSWA
T1589-p EEGsALKtSWAGAPV
A1594 LKtSWAGAPVHLGQG
K1605-ub LGQGQFLkFTQREGD
S1615-p QREGDREsWssGED_
S1617-p EGDREsWssGED___
S1618-p GDREsWssGED____
  mouse

 
Y22 LNRRLEAYLTRVKTL
K27 EAYLTRVKTLEEQNQ
K74 VDQRWREKHEAEVQR
T105-p VRLARERtIEEAACS
K120 RRALEAEKNARGWLS
T158-p LNAQAACtPRRPPAP
S169-p PPAPAHAsPIRAPEV
K260 DRLQATEKFQLAVEA
K272 VEALEQEKQGLQSQI
S277 QEKQGLQSQIAQILE
S295 QLAHLKMSLSLEVAT
S312 TLLEAENSRLQtPGR
T316-p AENSRLQtPGRSSQA
S321 LQtPGRSSQASLGFP
Q322 QtPGRSSQASLGFPD
- gap
S324 PGRSSQASLGFPDPK
- gap
K331 SLGFPDPKLKLHFLG
- gap
S347 PEDQHLGSVLPVLSP
S353 GSVLPVLSPTSFSsP
S356 LPVLSPTSFSsPLPN
S359-p LSPTSFSsPLPNTLE
T367-p PLPNTLEtPVTAFLK
T383 QEFLKARTPtLASTP
T385-p FLKARTPtLASTPIP
S388 ARTPtLASTPIPPMS
T389 RTPtLASTPIPPMSE
M394 ASTPIPPMSEAPyPK
Y399-p PPMSEAPyPKNAEVR
S413 RAQDVPHSLLQGGRQ
- gap
- gap
- gap
S469 PPLNPHHSILEAKDR
- gap
S478-p LEAKDREssESRVss
S479-p EAKDREssESRVssI
S481 KDREssESRVssIFQ
S484-p EssESRVssIFQEEE
S485-p ssESRVssIFQEEEG
K500 QIWELVKKEAATEVK
K507 KEAATEVKVENSLAQ
S511 TEVKVENSLAQEIQE
- gap
S529 DTEEIQDSQGPLQME
T537 QGPLQMETLEALGDE
- gap
S565-p GKENCNSsIEENsGT
S570-p NSsIEENsGTVKsPE
S575-p ENsGTVKsPEKEKQT
K585-ac KEKQTPLkSLEEkNV
K590-ac PLkSLEEkNVEAEkT
- gap
K596-ac EkNVEAEkTLENGVL
T597 kNVEAEkTLENGVLE
S623-p MEDQELMsPEHTLET
S688-p EDQRFPRsPEEDQQA
- gap
R697 EEDQQAFRPLEKENQ
- gap
S728-p IEKERQEsLKsPEEE
S731-p ERQEsLKsPEEEDQQ
S772-p IEKERQEsLKsPEEE
S775-p ERQEsLKsPEEEDQQ
S813-p ERLVEKEsQEsLKsP
S816-p VEKEsQEsLKsPEEE
S819-p EsQEsLKsPEEEDQR
S862-p KNQRPLRsLEVEEEE
- gap
- gap
S880-p VKPLEKVsQVsLEsL
S883-p LEKVsQVsLEsLEKE
S886-p VsQVsLEsLEKENVQ
S894-p LEKENVQsPRYLEED
S919-p KTHEILGsLEDRNGE
S957-p VNHLEKEsQEFLRsP
S963-p EsQEFLRsPEAEEEE
S977-p EEQVMVRsLEGENHD
S1010-p ESQDSRKsLEDESQE
S1021-p ESQETFGsLEKENLE
S1032 ENLESLRSLAGQDQE
T1060 AVEDEQMTVNPPEKV
P1074 VDPELPKPLRNDQEV
G1100 LVSLNEGGMETVKSS
K1105 EGGMETVKSSETENI
S1106 GGMETVKSSETENIE
T1109 ETVKSSETENIEsLE
S1114-p SETENIEsLEtVGEC
- gap
T1117-p ENIEsLEtVGECLGR
R1124 tVGECLGRRKsVDtQ
S1127-p ECLGRRKsVDtQEPL
T1130-p GRRKsVDtQEPLWST
S1136 DtQEPLWSTEVTSET
- gap
T1166-p DENQEVLtPLERESQ
S1177 RESQELRSLGKWNPE
S1188-p WNPETVEsPGGVEDS
- gap
S1195 sPGGVEDSQQCLEVE
R1207 EVEEGPEREQHQEsL
H1210 EGPEREQHQEsLRsL
S1213-p EREQHQEsLRsLGEV
S1216-p QHQEsLRsLGEVEWE
S1227-p VEWELPGsGsQQRWE
S1229-p WELPGsGsQQRWEDV
- gap
S1290 GEAWSLGSSEPKEQR
S1342-p AQAGEQDsVEVTLGL
S1430 ECKGWEESGEGWGEE
S1481-p PKLLEPCsPIPILTD
P1482 KLLEPCsPIPILTDA
- gap
- gap
S1541-p WEEGREDsEADELGE
T1554-p GETLPDStPLGLYLK
S1562-p PLGLYLKsPAsPKWE
S1565-p LYLKsPAsPKWEQAG
- gap
S1656 CWDQGGESDGFADEE
S1665 GFADEEESGEEGEEE
- gap
S1692 QWWGPGPSGGGVKVQ
- gap
- gap
- gap
- gap
S1738 VTALETVSQDSAEPS
S1741 LETVSQDSAEPSGSE
S1745 SQDSAEPSGSEGSES
S1747 DSAEPSGSEGSESAS
S1750 EPSGSEGSESASLEG
- gap
- gap
- gap
S1837-p LKAPLVGsPVHLGPS
K1848 LGPSQPLKFTLSGVD
S1858-p LSGVDGDsWssGED_
S1860-p GVDGDsWssGED___
S1861-p VDGDsWssGED____
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