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PRAS40 (human)

Overview PRAS40 plays a role in phosphatidylinositol 3-kinase (PI3K)/AKT survival signaling. PRAS40 is a physiological target of in vivo insulin action. Hyperinsulinemia increased its phosphorylation in human skeletal muscle biopsies. Phosphorylated PRAS40 is predominantly localized to the nucleus. In rats fed a high-fat diet (HFD), phosphorylation of PRAS40 was markedly reduced when compared with low-fat diet-fed animals in all tissues examined. A novel mTOR binding partner that mediates Akt signals to mTOR. Binding of PRAS40 inhibits mTOR activity and suppresses constitutive activation of mTOR in cells lacking TSC2. Phosphorylation by Akt and perhaps related kinases leads to its binding to 14-3-3. Two alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB. Protein type: Apoptosis Cellular Component: cytosolic part; nucleus; cytosol Molecular Function: protein binding Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of TOR signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; regulation of neuron apoptosis; negative regulation of protein kinase activity; negative regulation of cell size Reference #:  Q96B36 (UniProtKB) Alt. Names/Synonyms: AKT1S1; proline-rich AKT1 substrate 1 Gene Symbols: AKT1S1 Molecular weight: 27,383 Da Basal Isoelectric point: 4.65  Predict pI for various phosphorylation states CST Pathways:  Autophagy Signaling  |  Insulin Receptor Signaling  |  mTOR Signaling  |  PI3K/Akt Signaling  |  T Cell Receptor Signaling  |  Translation: eIF4E and p70S6K Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below PRAS40

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	Sites Implicated In
inhibition: S221‑p, T246‑p molecular association, regulation: S183‑p, S221‑p, T246‑p phosphorylation: T246‑p

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human ► Hide Isoforms
0	1		-	gap
0	1		T32-p	GTELVLLtAAPPPPP
0	3		R51-m1	CAYAAHGrGALAEAA
0	1		R51-m2	CAYAAHGrGALAEAA
0	1		T73-p	ALAHRAAtAARPPAP
0	26		S88-p	PPAPQPPsPtPsPPR
0	4		T90-p	APQPPsPtPsPPRPt
0	26		S92-p	QPPsPtPsPPRPtLA
0	4		T97-p	tPsPPRPtLAREDNE
0	4		S116	EPTETETSGEQLGIS
4	47		S183-p	PTQQYAKsLPVSVPV
0	13		T198-p	WGFKEKRtEARssDE
1	100		S202-p	EKRtEARssDEENGP
1	112		S203-p	KRtEARssDEENGPP
0	60		S211-p	DEENGPPssPDLDRI
1	139		S212-p	EENGPPssPDLDRIA
1	3		S221-p	DLDRIAAsMRALVLR
22	756		T246-p	LPRPRLNtsDFQKLK
0	54		S247-p	PRPRLNtsDFQKLKR
0	3		Y256-p	FQKLKRKy_______

	PRAS40 iso3
S2-p	______MsFEGGDGA
T52	GTELVLLTAAPPPPP
R71	CAYAAHGRGALAEAA
R71	CAYAAHGRGALAEAA
T93	ALAHRAATAARPPAP
S108	PPAPQPPSPTPSPPR
T110	APQPPSPTPSPPRPT
S112	QPPSPTPSPPRPTLA
T117	TPSPPRPTLAREDNE
S136	EPTETETSGEQLGIS
S203	PTQQYAKSLPVSVPV
T218	WGFKEKRTEARSSDE
S222	EKRTEARSSDEENGP
S223	KRTEARSSDEENGPP
S231	DEENGPPSSPDLDRI
S232	EENGPPSSPDLDRIA
S241	DLDRIAASMRALVLR
T266	LPRPRLNTSDFQKLK
S267	PRPRLNTSDFQKLKR
Y276	FQKLKRKY_______

	mouse
-	gap
T32	GTELVLLTAAPPPPP
R51	CAYAAHGRGALAEAA
R51	CAYAAHGRGALAEAA
T73	AQAHRAATATRPPGP
S88-p	PPAPQPPsPAPsPPP
A90	APQPPsPAPsPPPRP
S92-p	QPPsPAPsPPPRPAL
A98	PsPPPRPALAREDEE
S117-p	EPTETETsGERLGGS
S184-p	PTQQYAKsLPVSVPV
T199-p	WAFKEKRtEARssDE
S203-p	EKRtEARssDEENGP
S204-p	KRtEARssDEENGPP
S212-p	DEENGPPssPDLDRI
S213-p	EENGPPssPDLDRIA
S222-p	DLDRIAAsMRALVLR
T247-p	LPRPRLNtsDFQKLK
S248-p	PRPRLNtsDFQKLKR
Y257-p	FQKLKRKy_______

	rat
-	gap
T32	GTELVLLTAAPPPPP
R51	CAYAAHGRGALAEAA
R51	CAYAAHGRGALAEAA
T73	AQAHRAATATRPPGP
S88	PPAPQPPSPAPSSPP
A90	APQPPSPAPSSPPRP
S92	QPPSPAPSSPPRPAL
A98	PSSPPRPALAREEDE
S117	EPTETETSGERLGGS
S184-p	PTQQYAKsLPVSVPV
T199	WAFKEKRTEARssDE
S203-p	EKRTEARssDEENGP
S204-p	KRTEARssDEENGPP
S212-p	DEENGPPssPDLDRI
S213-p	EENGPPssPDLDRIA
S222	DLDRIAASMRALVLR
T247-p	LPRPRLNtSDFQKLK
S248	PRPRLNtSDFQKLKR
Y257	FQKLKRKY_______

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