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DIABLO (human)

Overview DIABLO Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance. Homodimer. Interacts with NGFRAP1/BEX3. Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin. Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Mitochondrial; Apoptosis Cellular Component: internal side of plasma membrane; mitochondrion; mitochondrial intermembrane space; cytosol Molecular Function: protein binding Biological Process: caspase activation; induction of apoptosis via death domain receptors; apoptosis; induction of apoptosis; caspase activation via cytochrome c; induction of apoptosis by oxidative stress Reference #:  Q9NR28 (UniProtKB) Alt. Names/Synonyms: 0610041G12Rik; DBLOH; DIABLO; diablo homolog (Drosophila); Diablo homolog, mitochondrial; DIABLO-S; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMAC3 Gene Symbols: DIABLO Molecular weight: 27,131 Da Basal Isoelectric point: 5.68  Predict pI for various phosphorylation states CST Pathways:  Apoptosis  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  Mitochondrial Control of Apoptosis Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below DIABLO 1FEW - A=56-239 (human) 1G3F - B=56-64 (human) 1G73 - A/B=56-217 (human) 1TW6 - C/D=56-64 (human) 1XB0 - G/H/I/J/K/L=56-62 (human) 1XB1 - G/H/I/J/K/L=56-62 (human) 3UIH - Q/P=56-70 (human) 3UIJ - Q/P=56-70 (human)

STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  RCSB PDB 1FEW 1G3F 1G73 1TW6 1XB0 1XB1 3UIH 3UIJ  |  Phospho3D  |  DISEASE  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene

	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			human
0	1		Y114	VYTLTSLYRQYTSLL
0	9		K123-u	QYTSLLGkMNSEEED
0	5		K146-u	ARAEMTSkHQEYLKL
0	18		K191-u	RNHIQLVkLQVEEVH
0	2		K203-u	EVHQLSRkAETkLAE
0	3		K207-u	LSRkAETkLAEAQIE
0	2		E214	kLAEAQIEELRQktQ
0	6		K219-u	QIEELRQktQEEGEE
0	14		T220-p	IEELRQktQEEGEER
0	1		G224	RQktQEEGEERAEsE
0	1		S230-p	EGEERAEsEQEAYLR

	mouse
Y112	VYTLVSLYRQYTSLL
K121	QYTSLLGKMNSQEED
K144	ARVEMTSKQQEYLKL
K189-u	RNHIQLVkSQVQEVR
K201	EVRQLSQKAETKLAE
K205	LSQKAETKLAEAQTk
K212-u	KLAEAQTkELHQkAQ
K217-u	QTkELHQkAQEVsDE
A218	TkELHQkAQEVsDEG
S222-p	HQkAQEVsDEGADQE
Q228	VsDEGADQEEEAYLR

	rat
Y112-p	VYTLVSLyRQYTSLL
K121	QYTSLLGKMNSQEED
K144	ARVEMTSKQQEYLKL
K189	RNHIQLVKSQVQEVR
K201	EVRQLSQKAETKLAE
K205	LSQKAETKLAEVQTQ
Q212	KLAEVQTQELRQKTQ
K217	QTQELRQKTQEASDE
T218	TQELRQKTQEASDEA
S222	RQKTQEASDEAADQE
Q228	ASDEAADQEEEAYLR

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