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Protein Page:
CUBN (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
CUBN Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected. Note: This description may include information from UniProtKB.
Cellular Component: Golgi apparatus; lysosomal lumen; brush border membrane; lysosomal membrane; endoplasmic reticulum; coated pit; cytosol; extrinsic to external side of plasma membrane; membrane; endocytic vesicle; apical plasma membrane; plasma membrane; endosome membrane
Molecular Function: protein binding; protein homodimerization activity; transporter activity; receptor activity; calcium ion binding; cobalamin binding
Biological Process: steroid metabolic process; receptor-mediated endocytosis; cholesterol metabolic process; vitamin metabolic process; lipoprotein transport; cobalamin metabolic process; cobalamin transport; tissue homeostasis; lipoprotein metabolic process; water-soluble vitamin metabolic process; vitamin D metabolic process
Reference #:  O60494 (UniProtKB)
Alt. Names/Synonyms: 460 kDa receptor; Cubilin; cubilin (intrinsic factor-cobalamin receptor); CUBN; FLJ90055; FLJ90747; gp280; IFCR; Intestinal intrinsic factor receptor; intrinsic factor B12-receptor; Intrinsic factor-cobalamin receptor; Intrinsic factor-vitamin B12 receptor; MGA1
Gene Symbols: CUBN
Molecular weight: 398,736 Da
Basal Isoelectric point: 5.14  Predict pI for various phosphorylation states
Select Structure to View Below

CUBN

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S107-p IGLPQNIssQIyQLN
0 1 S108-p GLPQNIssQIyQLNs
0 1 Y111-p QNIssQIyQLNsKLV
0 1 S115-p sQIyQLNsKLVDLER
0 1 S669-p GKFCTTFsVPPLQTT
0 1 S1016 IPPSLTSSGNSLMLV
0 1 Y1350-p GPRQMGRyCGVDLPP
0 1 T1804 EIREGNATGHLVGRY
0 1 K1909-m2 IQNCYYDkLRIYDGP
0 1 S2075-p YMFIRFTsDssVTRA
0 1 S2077-p FIRFTsDssVTRAGF
0 2 S2078-p IRFTsDssVTRAGFN
0 1 Y2108-p GIITSPKyPETYPSN
0 1 S2538 NVSNEIKSSGNTMKV
0 1 T3008 DEMPAPLTIAGPVLL
0 1 Y3018 GPVLLNFYSNEQITD
0 1 I3023 NFYSNEQITDFGFKF
0 1 S3031 TDFGFKFSYRIISCG
0 1 Y3032 DFGFKFSYRIISCGG
0 1 S3115-p LLGKFCGsKRPPNVK
0 1 T3139-p FKTDSFQtAKGWKMS
0 1 Y3486-p FSQNNELyLRFKsDs
0 1 S3491-p ELyLRFKsDsVtSDR
0 1 S3493-p yLRFKsDsVtSDRGy
0 1 T3495-p RFKsDsVtSDRGyEI
0 1 Y3500-p sVtSDRGyEIIWTSS
0 1 S3509-p IIWTSSPsGCGGTLy
0 3 Y3516-p sGCGGTLyGDRGSFT
  CUBN iso2  
- gap
- gap
- gap
- gap
S540 GKFCTTFSVPPLQTT
S887 IPPSLTSSGNSLMLV
Y1221 GPRQMGRYCGVDLPP
T1675 EIREGNATGHLVGRY
K1780 IQNCYYDKLRIYDGP
S1946 YMFIRFTSDSSVTRA
S1948 FIRFTSDSSVTRAGF
S1949 IRFTSDSSVTRAGFN
Y1979 GIITSPKYPETYPSN
S2409 NVSNEIKSSGNTMKV
T2879 DEMPAPLTIAGPVLL
Y2889 GPVLLNFYSNEQITD
I2894 NFYSNEQITDFGFKF
S2902 TDFGFKFSYRIISCG
Y2903 DFGFKFSYRIISCGG
S2986 LLGKFCGSKRPPNVK
T3010 FKTDSFQTAKGWKMS
Y3357 FSQNNELYLRFKSDS
S3362 ELYLRFKSDSVTSDR
S3364 YLRFKSDSVTSDRGY
T3366 RFKSDSVTSDRGYEI
Y3371 SVTSDRGYEIIWTSS
S3380 IIWTSSPSGCGGTLY
Y3387 SGCGGTLYGDRGSFT
  mouse

 
L104 TGLPQNILSQVHQLN
S105 GLPQNILSQVHQLNS
H108 QNILSQVHQLNSKLV
S112 SQVHQLNSKLVDLER
S669 GKFCTSLSTPPLQTT
S1016-p IPPSLTSsSHSIKLI
Y1350 GPQRIGRYCGENIPP
T1804-p EIREGNAtGHLMGRY
S1909 TTNCFYDSLKIYDGF
S2075 YMYIRFTSDGSVTGA
G2077 YIRFTSDGSVTGAGF
S2078 IRFTSDGSVTGAGFN
Y2108 GIITSPKYPDNYLPN
S2538-p NVTNEFKsSGNTMKV
T3008 SETLRPLTIDGPVML
Y3018 GPVMLNFYSDAYITD
I3023 NFYSDAYITDFGFKI
S3031 TDFGFKISYRVANCG
Y3032 DFGFKISYRVANCGG
S3115 SIGKFCGSTLPQVIK
T3139 FKTDSSQTARGWKVS
Y3486 FSQSNELYLHFHSDD
S3491 ELYLHFHSDDSDTHH
S3494 LHFHSDDSDTHHGYE
T3496 FHSDDSDTHHGYEII
Y3500 DSDTHHGYEIIWASS
T3509 IIWASSPTGCGGTLL
L3516 TGCGGTLLGNEGILA
  rat

 
L104 TGLPQNILSQVHQLN
S105 GLPQNILSQVHQLNS
H108 QNILSQVHQLNSKLV
S112 SQVHQLNSKLVDLER
S669 GKFCTSLSTPPLKTT
N1016 IPPSLTSNSNSIKLI
Y1350 GPQWMGRYCGNNMPP
T1804 EIREGNATGHLIGRY
S1909 TTNCFYDSLKIYDGF
S2075 YMYIRFTSDTSVAGT
T2077 YIRFTSDTSVAGTGF
S2078 IRFTSDTSVAGTGFN
Y2108 GVITSPKYPDTYLPN
S2538 NVTNEFKSSGNTMKV
T3008-p SETLRPLtVDGPVLL
Y3018-p GPVLLNFySDAYtTD
T3023-p NFySDAYtTDFGFKI
S3031-p TDFGFKIsyRAITCG
Y3032-p DFGFKIsyRAITCGG
S3115 SLGKFCGSTRPQTVK
T3139 FKTDSSQTARGWKIF
Y3486 FSQSNELYLHFHSDH
S3491 ELYLHFHSDHSVTNN
S3494 LHFHSDHSVTNNGYE
T3496 FHSDHSVTNNGYEII
Y3500 HSVTNNGYEIIWTSS
A3509 IIWTSSAAGCGGTLL
L3516 AGCGGTLLGDEGIFT
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