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Protein Page:
GAK (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
GAK Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Note: This description may include information from UniProtKB.
Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; EC 2.7.11.1; Other group; NAK family
Cellular Component: Golgi apparatus; focal adhesion; membrane; intracellular membrane-bound organelle; perinuclear region of cytoplasm
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding
Biological Process: cell cycle; protein amino acid phosphorylation
Reference #:  O14976 (UniProtKB)
Alt. Names/Synonyms: cyclin G associated kinase; Cyclin-G-associated kinase; FLJ16629; FLJ40395; GAK; MGC99654
Gene Symbols: GAK
Molecular weight: 143,191 Da
Basal Isoelectric point: 5.49  Predict pI for various phosphorylation states
Select Structure to View Below

GAK

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S16-p DFLAGPGsLGGASGR
0 6 S73-p YALKRLLsNEEEKNR
0 2 K90-ub IQEVCFMkKLSGHPN
0 1 K135 GQLVEFLKKMESRGP
0 1 Y153-p DTVLKIFyQTCRAVQ
0 1 K175-ub PIIHRDLkVENLLLS
0 1 Y201-p SATTISHyPDySWSA
0 8 Y204-p TISHyPDySWSAQRR
0 1 Y235-p TPEIIDLySNFPIGE
0 1 Y276-p LRIVNGKysIPPHDT
0 1 S277-p RIVNGKysIPPHDTQ
0 1 Y285-p IPPHDTQyTVFHsLI
0 1 S290-p TQyTVFHsLIRAMLQ
0 1 K325-ub AARNVNPkSPITELL
0 1 Y338-p LLEQNGGyGsATLSR
0 1 S340-p EQNGGyGsATLSRGP
0 26 Y367-p GGLALAEyDQPyGGF
0 29 Y371-p LAEyDQPyGGFLDIL
0 1 K390-ub ERLFTNLkDTSSkVI
0 1 K395-ac NLkDTSSkVIQSVAN
0 17 Y403-p VIQSVANyAkGDLDI
0 3 K405-ub QSVANyAkGDLDISY
0 1 S421-p TSRIAVMsFPAEGVE
0 2 K432-ub EGVESALkNNIEDVR
0 1 S456-p HYAVYNLsPRTYRPS
0 1 S540-p EAAVYMFsMKRCPPG
0 3 Y615-p VASTSQEyDKMRDFK
0 1 K685 ATTVKFAKYDLDACD
0 1 Y764-p QPGSTAQyDAGAGsP
0 2 S770-p QyDAGAGsPEAEPtD
0 4 T776-p GsPEAEPtDsDsPPs
0 1 S778-p PEAEPtDsDsPPsSs
0 4 S780-p AEPtDsDsPPsSsAD
0 2 S783-p tDsDsPPsSsADASR
0 1 S785-p sDsPPsSsADASRFL
0 4 T794-p DASRFLHtLDWQEEK
0 1 S811-p ETGAENAsSKESEsA
0 2 S817-p AsSKESEsALMEDRD
0 24 S826-p LMEDRDEsEVsDEGG
0 25 S829-p DRDEsEVsDEGGsPI
0 7 S834-p EVsDEGGsPIssEGQ
0 1 S837-p DEGGsPIssEGQEPR
0 2 S838-p EGGsPIssEGQEPRA
0 1 P849 EPRADPEPPGLAAGL
0 1 T908-p CKAPSSNtDLLSCLL
0 1 S939-p EDPLLLAsPAPPLSV
0 1 A1039 DLLGGWAAWTETAAS
0 1 S1096-p LSSGLQGsPAGFPPG
0 1 S1118-p TTPKGSSsWQTSRPP
0 1 R1123 SSsWQTSRPPAQGAs
0 2 S1130-p RPPAQGAsWPPQAKP
0 2 Y1149-p CTQPRPNyASNFsVI
0 1 S1154-p PNyASNFsVIGAREE
0 1 S1168 ERGVRAPSFAQKPKV
0 1 S1176-p FAQKPKVsENDFEDL
0 1 S1185-p NDFEDLLsNQGFSSR
0 1 T1213-p KQDLAKDtDPLKLKL
0 1 K1227 LLDWIEGKERNIRAL
  mouse

 
S16 DFLAGPGSLGGAAGR
S73 YALKRLLSNEEEKNR
K90-ub IQEVCFLkKLSGHPN
K135-ub GQLVEFLkRVECKGP
Y153 DSILKIFYQTCRAVQ
K175 PIIHRDLKVENLLLS
Y201 SATTISHYPDYSWSA
Y204 TISHYPDYSWSAQKR
Y235 TPEIVDLYSNFPIGE
Y276 LRIVNGKYSIPVNDT
S277 RIVNGKYSIPVNDTR
Y285 IPVNDTRYTVFHDLI
D290 TRYTVFHDLIRAMLK
K325 AARNVNPKAPITELL
Y338 LLEQNGGYGNSGPSR
N340 EQNGGYGNSGPSRAQ
Y365 GVLALAEYDQPYGGF
Y369 LAEYDQPYGGFLDIL
K388 ERLFTNLKDTSSKVI
K393 NLKDTSSKVIQSVAN
Y401 VIQSVANYAKGDLDI
K403 QSVANYAKGDLDISY
S419 TSRIAVMSFPAEGVE
K430 EGVESAIKNNIEDVR
S454 HYAVYNLSPRIYRAS
S538 EAAVYMFSMKRCPPG
Y613 VTTTSQEYDRMKEFK
K683-ac ATTVKFAkYDLDACD
Y762 QPGSTAQYDAEAGSP
S768 QYDAEAGSPEAEITE
T774 GSPEAEITESDSPQS
S776 PEAEITESDSPQSSS
S778 AEITESDSPQSSSTD
S781 TESDSPQSSSTDTNH
S783 SDSPQSSSTDTNHFL
T792 DTNHFLHTLDWQEEK
S809-p ETGLDNTsPKESQSV
S815 TsPKESQSVLIADGD
S824-p LIADGDGsEVsDEEE
S827-p DGDGsEVsDEEEAsF
S833-p VsDEEEAsFPsEERK
P835 DEEEAsFPsEERKPG
S836-p EEEAsFPsEERKPGA
T847-p KPGAGEDtPRLAAGT
T906 CGVPSSNTDLLSCLL
S938 EAPLLLASPVSPLGL
T1038-p DLLGGWDtWADTATP
L1095 LSSSLQGLPAGLPAG
P1117 PTQKSNSPWQANrPT
R1122-m1 NSPWQANrPTAPGTS
S1129 rPTAPGTSWTPQAKP
- gap
S1149 EQLRSHFSVIGAREE
S1163-p ERGVRVPsFAQKPKV
S1171 FAQKPKVSENDFEDL
P1180 NDFEDLLPNQGFSKS
T1207 KQELARDTDPLKLKL
K1221-ub LLDWIEGkERNIRAL
  rat

 
S16 DFLAGPGSLGGAAGR
S73 YALKRLLSNEEEKNR
K90 IQEVCFLKKLSGHPN
R135 GQLVEFLRRVECKGP
Y153 DSILKIFYQTCRAVQ
K175 PIIHRDLKVENLLLS
Y201 SATTISHYPDYSWSA
Y204 TISHYPDYSWSAQKR
Y235 TPEIVDLYSNFPIGE
Y276 LRIVNGKYSIPVNDT
S277 RIVNGKYSIPVNDTR
Y285 IPVNDTRYTVFHDLI
D290 TRYTVFHDLIRGMLK
K325 AARNVNPKAPITELL
Y338 LLEQNGGYGNSGPSR
N340 EQNGGYGNSGPSRAQ
Y365 GVLALAEYDQPYGGF
Y369 LAEYDQPYGGFLDIL
K388 ERLFTNLKDTSSKVI
K393 NLKDTSSKVIQSVAN
Y401 VIQSVANYAKGDLDI
K403 QSVANYAKGDLDISY
S419 TSRIAVMSFPAEGVE
K430 EGVESAIKNNIEDVR
S454 HYAVYNLSPRIYRAS
S538 EAAVYMFSMKRCPPG
Y613 VTTTSQEYDRMKEFK
K683 ATTVKFAKYDLDACD
Y762 QPGSTAQYDAEAGSP
S768 QYDAEAGSPEAEITE
T774 GSPEAEITESDSPQS
S776 PEAEITESDSPQSSS
S778 AEITESDSPQSSSTD
S781 TESDSPQSSSTDTNH
S783 SDSPQSSSTDTNHFL
T792 DTNHFLHTLDWQEEK
S809 ETGVDNTSPKESQSN
S815 TSPKESQSNLIADGD
S824-p LIADGDGsEVsDEEE
S827-p DGDGsEVsDEEEASC
S833 VsDEEEASCPSEERK
P835 DEEEASCPSEERKPG
S836 EEEASCPSEERKPGA
T847 KPGAGEDTPRLAAGT
T906 SGVQSSNTDLLSSLL
S938 ETPLLLASPVSLLGV
T1038 DLLGGWDTWAETALP
L1095 LSSSLQGLPAGLPAG
S1117 TTHKSNSSWQTTRPT
R1122 NSSWQTTRPTAPGTS
S1129 RPTAPGTSWPPQAKP
- gap
S1149 EQLRSHFSVIGAREE
S1163 ERGVRAPSFAQKPKV
S1171 FAQKPKVSENDFEDL
P1180 NDFEDLLPNQGFSKS
T1207 KQELARDTDPFKLKL
K1221 LLDWIEGKERNIRAL
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