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Protein Page:
BUB1B (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitylation
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
BUB1B Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, Other; EC 2.7.11.1; Other group; BUB family
Chromosomal Location of Human Ortholog: 15q15
Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity
Biological Process: mitosis; cell proliferation; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; apoptosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition
Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait
Reference #:  O60566 (UniProtKB)
Alt. Names/Synonyms: Bub1A; BUB1B; BUB1beta; BUBR1; budding uninhibited by benzimidazoles 1 homolog beta (yeast); hBUBR1; MAD3/BUB1-related protein kinase; MAD3L; Mitotic checkpoint kinase MAD3L; Mitotic checkpoint serine/threonine-protein kinase BUB1 beta; Protein SSK1; SSK1
Gene Symbols: BUB1B
Molecular weight: 119,545 Da
Basal Isoelectric point: 5.2  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

BUB1B

Protein Structure Not Found.


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Sites Implicated In
cell cycle regulation: T620‑p, S670‑p, S676‑p, T680‑p, T792‑p, T1008‑p, S1043‑p
cytoskeletal reorganization: T620‑p, S670‑p, S676‑p
enzymatic activity, induced: T792‑p, T1008‑p
molecular association, regulation: T620‑p, S670‑p, S676‑p, T680‑p
protein stabilization: T620‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 LTP 

LTP: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 HTP 

HTP: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 S12 KKEGGALSEAMSLEG
0 6 S39-p LRQGRIMstLQGALA
0 1 T40-p RQGRIMstLQGALAQ
1 1 T54-p QESACNNtLQQQKRA
0 1 S83-p DVWDRYIsWTEQNYP
0 1 K113-ub VEALQGEkRYYSDPR
0 1 K210-ub QTLLALEkEEEEEVF
0 1 S232 STLAELKSKGKKTAR
4 0 K250-ac IRVGGALkAPSQNRG
1 0 K250-sm IRVGGALkAPSQNRG
0 1 K250-ub IRVGGALkAPSQNRG
0 3 T315-p LQAGPWNtGRSLEHR
0 6 S367-p PSINHILstRKPGKE
0 1 T368-p SINHILstRKPGKEE
0 7 S384-p DPLQRVQsHQQASEE
0 15 Y404-p MYCKEKIyAGVGEFS
0 2 T434-p QREAELLtsAEKRAE
2 6 S435-p REAELLtsAEKRAEM
0 1 K451-ac KQIEEMEkKLKEIQT
0 1 T471-p TGDQQEEtMPTkETT
0 1 K475-ub QEEtMPTkETTKLQI
0 1 S509 NCCARETSLAENIWQ
0 1 S521-p IWQEQPHsKGPSVPF
0 1 P524 EQPHsKGPSVPFSIF
0 1 S537-p IFDEFLLsEkKNKsP
0 2 K539-ub DEFLLsEkKNKsPPA
3 17 S543-p LsEkKNKsPPADPPR
2 2 S574-p ITSNEDVsPDVCDEF
0 7 T600-p ITGFRNVtICPNPED
0 7 T608-p ICPNPEDtCDFARAA
1 0 T620-p RAARFVStPFHEIMS
0 1 K629-ub FHEIMSLkDLPsDPE
0 1 S633-p MSLkDLPsDPERLLP
0 3 Y660-p QTACGTIySQTLsIK
0 1 S665-p TIySQTLsIKKLsPI
6 24 S670-p TLsIKKLsPIIEDsR
5 3 S676-p LsPIIEDsREAtHSs
3 0 T680-p IEDsREAtHSsGFSG
0 1 S683-p sREAtHSsGFSGSSA
0 1 A690 sGFSGSSASVASTSs
0 1 S694 GSSASVASTSsIKCL
0 1 S697-p ASVASTSsIKCLQIP
0 1 T710-p IPEKLELtNETSENP
2 4 S720-p TSENPTQsPWCSQYR
0 1 S733-p YRRQLLKsLPELSAS
0 1 Y766-p IELGNEDyCIKREYL
2 0 T792-p PRNSAELtVIKVsSQ
0 1 S797-p ELtVIKVsSQPVPWD
1 2 S884-p EIVHGDLsPRCLILR
0 2 K995-ub KDGELWNkFFVRILN
2 0 T1008-p LNANDEAtVSVLGEL
0 1 K1040-ub KALWKVGkLtsPGAL
1 2 T1042-p LWKVGkLtsPGALLF
3 11 S1043-p WKVGkLtsPGALLFQ
0 2 - gap
  BUB1B iso2  
S12 KKEGGALSEAMSLEG
S39 LRQGRIMSTLQGALA
T40 RQGRIMSTLQGALAQ
T54 QESACNNTLQQQKRA
S83 DVWDRYISWTEQNYP
- under review  
K156 QTLLALEKEEEEEVF
S178 STLAELKSKGKKTAR
K196 IRVGGALKAPSQNRG
K196 IRVGGALKAPSQNRG
K196 IRVGGALKAPSQNRG
T261 LQAGPWNTGRSLEHR
S313 PSINHILSTRKPGKE
T314 SINHILSTRKPGKEE
S330 DPLQRVQSHQQASEE
Y350 MYCKEKIYAGVGEFS
T380 QREAELLTSAEKRAE
S381 REAELLTSAEKRAEM
K397 KQIEEMEKKLKEIQT
T417 TGDQQEETMPTKETT
K421 QEETMPTKETTKLQI
S455 NCCARETSLAENIWQ
S467 IWQEQPHSKVSLSLG
P475 KVSLSLGPSVPFSIF
S488 IFDEFLLSEKKNKSP
K490 DEFLLSEKKNKSPPA
S494 LSEKKNKSPPADPPR
S525 ITSNEDVSPDVCDEF
T551 ITGFRNVTICPNPED
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S559 ICPNPEDSREATHSS
T563 PEDSREATHSSGFSG
S566 SREATHSSGFSGSSA
A573 SGFSGSSASVASTSS
S577 GSSASVASTSSIKCL
S580 ASVASTSSIKCLQIP
T593 IPEKLELTNETSENP
S603 TSENPTQSPWCSQYR
S616 YRRQLLKSLPELSAS
Y649 IELGNEDYCIKREYL
T675 PRNSAELTVIKVSSQ
S680 ELTVIKVSSQPVPWD
S767 EIVHGDLSPRCLILR
K878 KDGELWNKFFVRILN
T891 LNANDEATVSVLGEL
K923 KALWKVGKLTSPGAL
T925 LWKVGKLTSPGALLF
S926 WKVGKLTSPGALLFQ
- gap
  mouse

 
S5-p ___MAEAsEAMCLEG
S32 LRHGRVMSTLQGALA
T33 RHGRVMSTLQGALAK
A48 QESAGHTALQQQKRA
N77 DVWDRYINWTEQNYP
T107 IEALQGETRYYNDPR
N204 QAFLALGNEEEEALE
S225-p SSLAELKsRGKKMAR
K243-ac SRVGGALkAPGQSRG
K243 SRVGGALKAPGQSRG
K243 SRVGGALKAPGQSRG
T308 LQPGPWSTDRPAGRR
S360 PSINHVLSTRKPGRE
T361 SINHVLSTRKPGREE
S377 DPLQRVQSHQQGCEE
Y397-p MYCKEKIyAGVGEFS
T427 RREAELLTSAKKREE
S428 REAELLTSAKKREEM
R444 KQIEEMERRLKAMQA
K464 AGGQQEEKMPTEDPA
E468 QEEKMPTEDPARLQI
S502-p SSNPREIsPAENILQ
S514 ILQEQPDSKGsSMPF
S517-p EQPDSKGsSMPFSIF
S529 SIFDESLSDKKDKsP
K531 FDESLSDKKDKsPAT
S535-p LSDKKDKsPATGGPQ
S567 GTTNEDVSPDICDEL
T593 ITGFRNVTLCPNPED
T601 LCPNPEDTCDFARAA
T613 RAARLASTPFHEILS
K622 FHEILSSKGIAADPE
A626 LSSKGIAADPEGLLQ
H649 AEAHHTVHHQALIIK
I654 TVHHQALIIKKLsPI
S659-p ALIIKKLsPIIEESR
S665 LsPIIEESREAtHSS
T669-p IEESREAtHSSGFSR
S672 SREAtHSSGFSRSSs
S679-p SGFSRSSsSAPsTSS
S683-p RSSsSAPsTSSIKGF
S686 sSAPsTSSIKGFQLL
T699 LLEKLELTNDGAEDA
S709 GAEDAIQSPWCSQYR
S722 YRLQLLKSLLELSAF
Y755 IELGPEDYVIKQEHL
T781 PRSSAELTMIKASSQ
S786 ELTMIKASSQPIPWD
S873-p EIVHGDLsPRSLILR
K985 KDSELWNKFFVRILN
T998 LNASDKSTVSVLGEL
K1030 RALWKLGKTIsPEAL
I1032 LWKLGKTIsPEALLT
S1033-p WKLGKTIsPEALLTQ
S1050-p KQPGGSQsPA_____
  rat

 
S5 ___MAAASEATCVEG
A32 LRHGRIMATLQGALA
T33 RHGRIMATLQGALAQ
T48 QESACSTTLQQQKWA
N77 DVWDRYINWTEQNYP
K107 IEALQGEKRYYSDPR
N204 QALLALGNEEEEVLG
S225 STLAELKSRGKKMAR
K243 NRVGGALKAPGQSRG
K243 NRVGGALKAPGQSRG
K243 NRVGGALKAPGQSRG
T308 LQPGPWNTDRPLEYR
S360 PSINHVLSTRKPGRE
T361 SINHVLSTRKPGREE
S377 DPLQRVQSHQQGCEE
Y397 MYCKEKIYAGVGEFS
T427 RREAELLTSAKKREE
S428 REAELLTSAKKREEM
R444 KQIEEMERKLKAMQT
R464 AGDQQEERMLSKDSA
K468 QEERMLSKDSARLEI
S502 SSNPREISPAENILQ
T514 ILQEQPETKGPSMPF
P517 EQPETKGPSMPFSIF
S529 SIFDESLSDKKDKSP
K531 FDESLSDKKDKSPAT
S535 LSDKKDKSPATDGPQ
S567 IPANEDISPDVCDEL
I593 ITGFRNGILCPNPED
T601 LCPNPEDTCDFARAA
T613 RAARFASTPFHEILP
K622 FHEILPSKSIASDPK
S626 LPSKSIASDPKEGLQ
H649 TEGHHIVHLQTLSVK
S654 IVHLQTLSVKKLSPI
S659 TLSVKKLSPIIEDSR
S665 LSPIIEDSREATHSS
T669 IEDSREATHSSGFSR
S672 SREATHSSGFSRSSS
S679 SGFSRSSSSVTSTSS
S683 RSSSSVTSTSSIKGF
S686 SSVTSTSSIKGFQIL
T699 ILEKLELTTDAAENA
S709 AAENAIQSPWCSHYR
S722 YRLQLLKSLPELSAF
Y755 IELGHEDYCIKQEHL
T781 PRNSAELTMIKASSQ
S786 ELTMIKASSQPLPWD
S873 EIVHGDLSPRSLILR
K985 KDGELWNKFFVQILN
T998 LNASDKSTVFILREL
K1030 KALWKLGKVISPEAS
I1032 LWKLGKVISPEASLI
S1033 WKLGKVISPEASLIQ
- gap
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