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Protein Page:
JUB (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
JUB Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell- cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1- induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Interacts with GRB2, PIP5K1B and SLC1A2. Interacts with AURKA; the interaction occurs during mitosis and both proteins are phosphorylated as they form a complex. Interacts with CTNNA1 and with F-actin. Interacts with LATS2; the interaction occurs during mitosis and the complex regulates organization of the spindle apparatus through recruitment of TUBG to the centrosome. Forms a complex with SQSTM1, PRKCZ and TRAF6. Component of the GFI1-AJUBA-HDAC1 repressor complex. Interacts directly (via the LIM domains) with GFI1; the interaction results in the HDAC-dependent corepression of a subset of GFI1 target genes, and is independent of the GFI1 SNAG domain. Interacts with HDAC1, HDAC2 and HDAC3. Interacts with SNAI2/SLUG (via SNAG domain) and SCRT1 (via SNAG domain). Interacts with EIF4E, EIF2C1, EIF2C2, DCP2, DDX6, LATS1, LATS2, SAV1, EGLN2/PHD1 and EGLN3/PHD3. Interacts (via LIM domains) with isoform 1 and isoform 3 of VHL. Interacts (via LIM domains) with SNAI1 (via SNAG domain). Belongs to the zyxin/ajuba family. Note: This description may include information from UniProtKB.
Protein type: Transcription, coactivator/corepressor; Cell adhesion; Motility/polarity/chemotaxis
Cellular Component: transcription factor complex; focal adhesion; lamellipodium; plasma membrane; microtubule organizing center; intercellular junction; cytosol; nucleus
Molecular Function: actin filament binding; protein binding; zinc ion binding; chromatin binding; transcription corepressor activity; alpha-catenin binding
Biological Process: focal adhesion formation; lamellipodium biogenesis; negative regulation of kinase activity; regulation of GTPase activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; glycerophospholipid biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cellular biosynthetic process; miRNA-mediated gene silencing; regulation of cell migration; positive regulation of MAP kinase activity; positive regulation of protein complex assembly; calcium-dependent cell-cell adhesion; response to hypoxia; mitotic cell cycle; G2/M transition of mitotic cell cycle; wound healing, spreading of epidermal cells
Reference #:  Q96IF1 (UniProtKB)
Alt. Names/Synonyms: Ajuba; JUB; jub, ajuba homolog (Xenopus laevis); MGC15563; Protein ajuba
Gene Symbols: AJUBA
Molecular weight: 56,934 Da
Basal Isoelectric point: 6.86  Predict pI for various phosphorylation states
CST Pathways:  Adherens Junction Dynamics
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

JUB

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K7-m1 _MERLGEkASRLLEK
0 1 T30-p SRSGSDGtPGPGKGR
0 2 S39-p GPGKGRLsGLGGPRK
0 38 S69-p EPAREQGsLDAERNQ
0 4 S79-p AERNQRGsFEAPRYE
0 15 S119-p EPTAPALsPRSSFAs
0 1 S126-p sPRSSFAssSAsDAs
0 1 S127-p PRSSFAssSAsDAsK
0 3 S130-p SFAssSAsDAsKPss
0 3 S133-p ssSAsDAsKPssPRG
0 2 S136-p AsDAsKPssPRGSLL
0 7 S137-p sDAsKPssPRGSLLL
0 1 S159-p AGGSRPCsNRTSGIS
1 0 T162 SRPCsNRTSGISMGY
0 2 S175-p GYDQRHGsPLPAGPC
0 1 Y215-p HAALDRLyAQRPAGF
0 4 S230-p GCQESRHsyPPALGs
0 2 Y231-p CQESRHsyPPALGsP
0 2 S237-p syPPALGsPGALAGA
0 1 A248 LAGAGVGAAGPLERR
0 11 S263-p GAQPGRHsVtGYGDC
0 1 T265-p QPGRHsVtGYGDCAV
0 1 Y346-p IKCNKGIyGQSNACQ
  mouse

 
K7 _MERLGEKASRLLEK
T40 SRSGSDGTPGAGKGR
S49 GAGKGRLSGLGGPRK
P79 EPAREQGPLDAERNA
S89-p AERNARGsFEAQRFE
S129-p ETTAPALsPRSSFAS
S136 sPRSSFASSSASDAS
S137 PRSSFASSSASDASK
S140 SFASSSASDASKPss
S143 SSSASDASKPssPRG
S146-p ASDASKPssPRGSLL
S147-p SDASKPssPRGSLLL
S169 AGGSRPCSNRtSGIS
T172-p SRPCSNRtSGISMGY
S185-p GYDQRHGsPLPAGPC
C224 HAALDRLCAHRSVGF
S239-p GCQESRHsYPPALGS
Y240 CQESRHsYPPALGSP
S246 sYPPALGSPGALTGA
T257-p LTGAVVGtAGPLERR
S272-p GAQPGRHsVtGYGDC
T274-p QPGRHsVtGYGDCAA
Y355 IKCNKGIYGQSNACQ
  rat

 
K7 _MERLGEKASRLLEK
T40 SRSGSDGTPGAGKGR
S49 GAGKGRLSGLGGPRK
P79 EPAREQGPLDAERNA
S89 AERNARGSFEAQRFE
S129-p ETTAPALsPRSSFAS
S136 sPRSSFASSSASDAS
S137 PRSSFASSSASDASK
S140 SFASSSASDASKPSs
S143 SSSASDASKPSsPRG
S146 ASDASKPSsPRGSLL
S147-p SDASKPSsPRGSLLL
S169 AGGSRPCSNRTSGIS
T172 SRPCSNRTSGISMGY
S185 GYDQRHGSPLPAGPC
C225 HAALDRLCAHRPVGF
S240 GCQESRHSYPPALGS
Y241 CQESRHSYPPALGSP
S247 SYPPALGSPGALTGA
T258 LTGAVVGTAGPLERR
S273 GTQPGRHSVTGYGDC
T275 QPGRHSVTGYGDCAA
Y356 IKCNKGIYGQSNACQ
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