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Protein Page:
Ku80 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Ku80 the 80-kilodalton subunit of the Ku complex, also known as ATP-dependant DNA helicase II. A single stranded DNA-dependent ATP-dependent helicase. It functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by p70. Note: This description may include information from UniProtKB.
Protein type: EC 3.6.1.-; Helicase; Nuclear receptor co-regulator; EC 3.6.4.-; DNA binding protein
Cellular Component: nucleoplasm; nuclear telomere cap complex; nuclear chromosome, telomeric region; nucleolus; cytosol; nucleus
Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; ubiquitin protein ligase binding; double-stranded DNA binding; damaged DNA binding; telomeric DNA binding; double-stranded telomeric DNA binding; 5'-deoxyribose-5-phosphate lyase activity; ATP binding
Biological Process: response to drug; positive regulation of neurogenesis; viral reproduction; transcription, DNA-dependent; DNA repair; DNA duplex unwinding; double-strand break repair via nonhomologous end joining; DNA recombination; cell proliferation; double-strand break repair; positive regulation of interferon type I production; innate immune response; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance
Reference #:  P13010 (UniProtKB)
Alt. Names/Synonyms: 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; FLJ39089; G22P2; KARP-1; KARP1; Ku autoantigen, 80kD); Ku autoantigen, 80kDa; Ku80; Ku86; Ku86 autoantigen related protein 1; KUB2; Lupus Ku autoantigen protein p86; NFIV; Nuclear factor IV; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5; X-ray repair, complementing defective, repair in Chinese hamster; XRCC5
Gene Symbols: XRCC5
Molecular weight: 82,705 Da
Basal Isoelectric point: 5.55  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

Ku80

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 K36-ub SPFEQAKkVItMFVQ
0 1 T39-p EQAKkVItMFVQRQV
0 1 S102-p ESKIQPGsQQADFLD
0 3 K125-ub IQHETIGkkFEKRHI
0 1 K126-ub QHETIGkkFEKRHIE
0 1 K144-ac DLSSRFSksQLDIII
0 26 K144-ub DLSSRFSksQLDIII
0 13 S145-p LSSRFSksQLDIIIH
0 2 K155-ac DIIIHSLkkCDISLQ
0 2 K155-ub DIIIHSLkkCDISLQ
0 1 K155-sc DIIIHSLkkCDISLQ
0 12 K156-ub IIIHSLkkCDISLQF
0 4 K171-ub FLPFSLGkEDGSGDR
0 1 K195-ac HGPSFPLkGITEQQk
0 89 K195-ub HGPSFPLkGITEQQk
0 3 K202-ub kGITEQQkEGLEIVK
0 1 Y225-p EDGLDEIySFSESLR
0 5 K233-ub SFSESLRkLCVFkKI
0 1 K238-ub LRkLCVFkKIERHSI
0 3 S255-p PCRLTIGsNLsIRIA
0 2 S258-p LTIGsNLsIRIAAYk
0 4 K265-ac sIRIAAYkSILQERV
0 38 K265-ub sIRIAAYkSILQERV
0 1 K265-sc sIRIAAYkSILQERV
0 5 K274-ub ILQERVKkTWTVVDA
0 7 K282-ub TWTVVDAkTLKKEDI
0 1 K282-sc TWTVVDAkTLKKEDI
0 4 Y295-p DIQKETVyCLNDDDE
0 55 K307-ub DDETEVLkEDIIQGF
0 2 S318-p IQGFRYGsDIVPFsk
0 1 S324-p GsDIVPFskVDEEQM
0 19 K325-ub sDIVPFskVDEEQMk
0 2 K332-ac kVDEEQMkYksEGkC
0 4 K332-ub kVDEEQMkYksEGkC
0 1 K334-ub DEEQMkYksEGkCFs
0 1 S335-p EEQMkYksEGkCFsV
0 19 K338-ac MkYksEGkCFsVLGF
0 11 K338-ub MkYksEGkCFsVLGF
0 1 S341-p ksEGkCFsVLGFCkS
0 18 K347-ub FsVLGFCkSSQVQRR
0 1 K363-ac FMGNQVLkVFAARDD
0 7 K363-ub FMGNQVLkVFAARDD
0 3 K399-ac IVRYAYDkRANPQVG
0 4 Y416-p FPHIKHNyECLVYVQ
0 1 S437-p LRQYMFSsLkNSKkY
0 3 K439-ub QYMFSsLkNSKkYAP
0 1 K443-ac SsLkNSKkYAPTEAQ
0 2 K443-ub SsLkNSKkYAPTEAQ
0 1 D455-ca EAQLNAVdALIDSMS
0 2 K465-ub IDSMSLAkkDEkTDT
0 1 K466-ac DSMSLAkkDEkTDTL
0 1 K466-ub DSMSLAkkDEkTDTL
0 5 K469-ub SLAkkDEkTDTLEDL
0 67 K481-ub EDLFPTTkIPNPRFQ
0 1 T523-p LNPPAEVttkSQIPL
0 1 T524-p NPPAEVttkSQIPLS
0 6 K525-ub PPAEVttkSQIPLSk
0 1 I528 EVttkSQIPLSkIkt
0 1 K532-ac kSQIPLSkIktLFPL
0 59 K532-ub kSQIPLSkIktLFPL
0 5 K534-ub QIPLSkIktLFPLIE
0 1 K534-sc QIPLSkIktLFPLIE
0 4 T535-p IPLSkIktLFPLIEA
0 3 K543-ac LFPLIEAkkkDQVTA
0 8 K543-ub LFPLIEAkkkDQVTA
0 1 K543-sc LFPLIEAkkkDQVTA
0 3 K544-ub FPLIEAkkkDQVTAQ
0 1 K545-ub PLIEAkkkDQVTAQE
0 1 D556-ca TAQEIFQdNHEDGPT
0 28 K565-ac HEDGPTAkkLktEQG
0 1 K565-ub HEDGPTAkkLktEQG
0 13 K566-ac EDGPTAkkLktEQGG
0 2 K566-ub EDGPTAkkLktEQGG
1 0 K568-sm GPTAkkLktEQGGAH
0 1 T569-p PTAkkLktEQGGAHF
2 1 S577-p EQGGAHFsVSsLAEG
2 2 S580-p GAHFsVSsLAEGsVT
0 2 S585-p VSsLAEGsVTSVGSV
0 1 T629-p QFLDTNEtPYFMKSI
0 1 K648-ac AFREEAIkFSEEQRF
0 6 K648-ub AFREEAIkFSEEQRF
0 2 K660-ac QRFNNFLkALQEkVE
0 7 K660-ub QRFNNFLkALQEkVE
0 1 K665-ac FLkALQEkVEIKQLN
0 11 K665-ub FLkALQEkVEIKQLN
0 16 K702-ac SVTAEEAkKFLAPKD
0 1 K702-m1 SVTAEEAkKFLAPKD
0 3 K702-ub SVTAEEAkKFLAPKD
0 1 K702-sc SVTAEEAkKFLAPKD
0 1 S712-p LAPKDKPsGDtAAVF
2 1 T715-p KDKPsGDtAAVFEEG
  mouse

 
K36 SPIEQAKKVMTMFVQ
T39 EQAKKVMTMFVQRQV
S102 GNKIQPSSQQADFLD
K125 IQRETIGKKFGKKHI
K126 QRETIGKKFGKKHIE
Q144 DLSSPFSQDQLDVII
Q144 DLSSPFSQDQLDVII
D145 LSSPFSQDQLDVIIC
K155 DVIICNLKKSGISLQ
K155 DVIICNLKKSGISLQ
K155 DVIICNLKKSGISLQ
K156 VIICNLKKSGISLQF
K171 FLPFPIDKNGEPGER
K195 LKPSFPQKGLTEQQK
K195 LKPSFPQKGLTEQQK
K202 KGLTEQQKEGIRMVT
Y225 EDGLDEIYSFSESLR
Q233 SFSESLRQLCVFKKI
K238 LRQLCVFKKIERRSM
P255 PCQLTIGPNLSIKIV
S258 LTIGPNLSIKIVAYK
K265 SIKIVAYKSIVQEKF
K265 SIKIVAYKSIVQEKF
K265 SIKIVAYKSIVQEKF
K274 IVQEKFKKSWVVVDA
R282 SWVVVDARTLKKEDI
R282 SWVVVDARTLKKEDI
Y295 DIQKETVYCLNDDDE
K307 DDETEVSKEDTIQGY
S318 IQGYRYGSDIIPFSk
S324 GSDIIPFSkVDEEQM
K325-ub SDIIPFSkVDEEQMk
K332-ac kVDEEQMkYKSEGKC
K332 kVDEEQMKYKSEGKC
K334 DEEQMkYKSEGKCFS
S335 EEQMkYKSEGKCFSV
K338 MkYKSEGKCFSVLGF
K338 MkYKSEGKCFSVLGF
S341 KSEGKCFSVLGFCKS
K347 FSVLGFCKSSQVHRR
K363 FMGHQVLKVFAAKDD
K363 FMGHQVLKVFAAKDD
K399 IVRYAYDKRSNPQVG
Y416 FPYIKDAYECLVYVQ
S437 LRQYMFSSLKNNKKC
K439 QYMFSSLKNNKKCTP
K443 SSLKNNKKCTPTEAQ
K443 SSLKNNKKCTPTEAQ
D455 EAQLSAIDDLIDSMS
K465 IDSMSLVKKNEEEDI
K466 DSMSLVKKNEEEDIV
K466 DSMSLVKKNEEEDIV
E469 SLVKKNEEEDIVEDL
K481 EDLFPTSKIPNPEFQ
K523 LDPPTEMKAKCEsPL
A524 DPPTEMKAKCEsPLS
K525 PPTEMKAKCEsPLSK
S528-p EMKAKCEsPLSKVKT
K532 KCEsPLSKVKTLFPL
K532 KCEsPLSKVKTLFPL
K534 EsPLSKVKTLFPLTE
K534 EsPLSKVKTLFPLTE
T535 sPLSKVKTLFPLTEV
K544 FPLTEVIKKKNQVTA
K544-ub FPLTEVIkKKNQVTA
K544 FPLTEVIKKKNQVTA
K545 PLTEVIkKKNQVTAQ
K546 LTEVIkKKNQVTAQD
D557 TAQDVFQDNHEEGPA
K566 HEEGPAAKKYKTEKE
K566 HEEGPAAKKYKTEKE
K567 EEGPAAKKYKTEKEE
K567 EEGPAAKKYKTEKEE
K569 GPAAKKYKTEKEEDH
T570 PAAKKYKTEKEEDHI
S578 EKEEDHISISSLAEG
S581 EDHISISSLAEGNIT
N586 ISSLAEGNITKVGSV
T630 QFLDTNETLYFMKSM
Q649 AFREEAIQFSEEQRF
Q649 AFREEAIQFSEEQRF
E661 QRFNSFLEALREKVE
E661 QRFNSFLEALREKVE
K666 FLEALREKVEIKQLN
K666 FLEALREKVEIKQLN
T703 SITAEEATKFLAPKD
T703 SITAEEATKFLAPKD
T703 SITAEEATKFLAPKD
T703 SITAEEATKFLAPKD
K713 LAPKDKAKEDTTGPE
T716 KDKAKEDTTGPEEAG
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