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vinculin (rat)

Overview vinculin Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB. Protein type: Cell adhesion; Cytoskeletal protein; Motility/polarity/chemotaxis Cellular Component: costamere; protein complex; focal adhesion; actin filament; fascia adherens; intercellular junction; Z disc; actin cytoskeleton; cell-cell adherens junction; adherens junction; stress fiber; plasma membrane; sarcolemma Molecular Function: Rho GTPase binding; structural molecule activity; actin binding; alpha-catenin binding Biological Process: lamellipodium biogenesis; apical junction assembly; morphogenesis of an epithelium; cell adhesion; regulation of cell migration Reference #:  P85972 (UniProtKB) Alt. Names/Synonyms: Metavinculin; Vcl; VINC; Vinculin Gene Symbols: Vcl Molecular weight: 116,615 Da Basal Isoelectric point: 5.83  Predict pI for various phosphorylation states Protein-Specific Antibodies or siRNAs from Cell Signaling Technology®

Select Structure to View Below vinculin 1RKC - A=1-258 (human) 1RKE - A=1-258, B=973-1134 (human) 1SYQ - A=1-258 (human) 1TR2 - A/B=1-1134 (human) 1YDI - A=1-258 (human) 2GWW - A=1-258 (human) 2HSQ - A=1-258 (human) 2IBF - A=1-258 (human) 3MYI - A=959-1130 (human) 3RF3 - A/B=1-258 (human) 3S90 - A/B=1-252 (human) 3TJ5 - A=1-255 (human) 3TJ6 - A=1-257 (human) 3VF0 - A=856-1134 (human) 4DJ9 - A=1-258 (human) 4EHP - A=1-252 (human) 1QKR - A/B=974-1135 (chicken) 1ST6 - A=1-1135 (chicken) 1T01 - A=1-254 (chicken) 1U6H - A=1-259 (chicken) 1XWJ - A=1-259 (chicken) 1ZVZ - A=1-259 (chicken) 1ZW2 - A=1-259 (chicken) 1ZW3 - A=1-259 (chicken) 2GDC - A=1-266 (chicken) 3ZDL - B=1-32 (chicken) 4E17 - A=1-259 (chicken) 4E18 - A=1-259 (chicken)

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	Modification Sites and Domains	Show Modification Legend
Click here to view phosphorylation modifications only

	Modification Sites in Parent Protein, Orthologs, and Isoforms	Show Modification Legend

SS  SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.	MS  MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.			rat
0	1		K59	SNLVRVGKETVQTTE
0	3		K71	TTEDQILKRDMPPAF
1	56		Y100-p	QMLQSDPySVPARDy
0	7		S101	MLQSDPySVPARDyL
0	141		Y107-p	ySVPARDyLIDGSRG
0	2		S112	RDyLIDGSRGILSGT
0	1		K173	PGMTKMAKMIDERQQ
0	1		K216	MKIFVTTKNSKNQGI
0	26		K219	FVTTKNSKNQGIEEA
0	1		K228	QGIEEALKNRNFTVG
0	1		S260	WDEDAWASKDTEAMK
0	1		K261	DEDAWASKDTEAMKR
0	1		S272	AMKRALASIDSKLNQ
0	1		S275	RALASIDSKLNQAKG
0	1		K276	ALASIDSKLNQAKGW
0	1		K281	DSKLNQAKGWLRDPN
0	23		N288	KGWLRDPNAsPGDAG
0	26		S290-p	WLRDPNAsPGDAGEQ
0	1		K308	QILDEAGKVGELCAG
0	1		K316	VGELCAGKERREILG
0	1		T324	ERREILGTCKMLGQM
0	12		S346-p	RARGQGAsPVAMQKA
0	3		S357	MQKAQQVSQGLDVLT
0	1		K366	GLDVLTAKVENAARK
0	1		K381	LEAMTNSKQSIAKKI
1	1		K386	NSKQSIAKKIDAAQN
0	1		K387	SKQSIAKKIDAAQNW
0	6		S434	ERDDILRSLGEIAAL
0	3		K444	EIAALTSKLGDLRRQ
0	3		K464	PEARALAKQVATALQ
0	15		K476	ALQNLQTKTNRAVAN
0	2		K496	AAVHLEGKIEQAQRW
0	1		T508	QRWIDNPTVDDRGVG
0	1		S566	AARGEGESPQARALA
0	7		S579	LASQLQDSLKDLKTQ
0	2		K581	SQLQDSLKDLKTQMQ
0	1		K584	QDSLKDLKTQMQEAM
0	2		S600	QEVSDVFSDTTTPIK
0	1		T603	SDVFSDTTTPIKLLA
0	5		T604	DVFSDTTTPIKLLAV
0	1		R639	NFENHSGRLGATAEK
0	2		K655	AAVGAANKSTVEGIQ
0	1		S664	TVEGIQASVKTAREL
0	1		K666	EGIQASVKTARELTP
0	770		Y692-p	NPGNQAAyEHFETMK
0	13		T697	AAyEHFETMKNQWID
0	3		K699	yEHFETMKNQWIDNV
0	3		K699	yEHFETMKNQWIDNV
0	1		T719	LVDEAIDTKsLLDAS
0	1		K720	VDEAIDTKsLLDASE
0	18		S721-p	DEAIDTKsLLDASEE
0	1		S726	TKsLLDASEEAIKKD
0	1		K731	DASEEAIKKDLDKCK
0	1		T754	QMLVAGATSIARRAN
0	1		K768	NRILLVAKREVENSE
0	1		S774	AKREVENSEDPKFRE
0	1		K778	VENSEDPKFREAVKA
0	1		K784	PKFREAVKAASDELS
0	1		K784	PKFREAVKAASDELS
0	2		K792	AASDELSKTISPMVM
0	1		S795	DELSKTISPMVMDAK
0	1		K802	SPMVMDAKAVAGNIs
0	2		K802	SPMVMDAKAVAGNIs
0	2		S809-p	KAVAGNIsDPGLQKS
0	8		K815	IsDPGLQKSFLDSGy
0	2		S816	sDPGLQKSFLDSGyR
0	71		S820	LQKSFLDSGyRILGA
1	1614		Y822-p	KSFLDSGyRILGAVA
0	1		K830	RILGAVAKVREAFQP
0	1		S913	HDEARKWSSKGNDII
0	2		K915	EARKWSSKGNDIIAA
0	65		K924	NDIIAAAKRMALLMA
0	1		K924	NDIIAAAKRMALLMA
0	1		R925	DIIAAAKRMALLMAE
0	1		R935	LLMAEMSRLVRGGSG
0	1		K956	QCAKDIAKASDEVTR
0	1		K996	PTISTQLKILSTVKA
0	20		K1002	LKILSTVKATMLGRT
0	2		K1002	LKILSTVKATMLGRT
1	0		S1033	NAQNLMQSVKETVRE
0	1		K1035	QNLMQSVKETVREAE
1	0		S1045	VREAEAASIKIRTDA
0	1		T1055	IRTDAGFTLRWVRKT
5	21		Y1065	WVRKTPWYQ______

	human ► Hide Isoforms
K59	SNLVRVGKETVQTTE
K71-a	TTEDQILkRDMPPAF
Y100-p	QMLQSDPysVPARDy
S101-p	MLQSDPysVPARDyL
Y107-p	ysVPARDyLIDGsRG
S112-p	RDyLIDGsRGILSGT
K173-a	PGMTKMAkMIDERQQ
K216	MKIFVTTKNSkNQGI
K219-u	FVTTKNSkNQGIEEA
K228	QGIEEALKNRNFTVE
S260	WDEDAWASKDTEAMK
K261	DEDAWASKDTEAMKR
S272-p	AMKRALAsIDsKLNQ
S275-p	RALAsIDsKLNQAKG
K276	ALAsIDsKLNQAKGW
K281	DsKLNQAKGWLRDPs
S288-p	KGWLRDPsAsPGDAG
S290-p	WLRDPsAsPGDAGEQ
K308	QILDEAGKVGELCAG
K316-a	VGELCAGkERREILG
T324-p	ERREILGtCKMLGQM
S346-p	RARGQGSsPVAMQKA
S357-p	MQKAQQVsQGLDVLT
K366	GLDVLTAKVENAARK
K381	LEAMTNSKQSIAkKI
K386-u	NSKQSIAkKIDAAQN
K387	SKQSIAkKIDAAQNW
S434-p	ERDDILRsLGEISAL
K444-u	EISALTSkLADLRRQ
K464-u	PEARALAkQVATALQ
K476-u	ALQNLQTkTNRAVAN
K496-a	AAVHLEGkIEQAQRW
T508-p	QRWIDNPtVDDRGVG
S566-p	AARGEGEsPQARALA
S579-p	LASQLQDsLkDLKAR
K581-u	SQLQDsLkDLKARMQ
K584	QDsLkDLKARMQEAM
S600	QEVSDVFSDTTtPIK
T603	SDVFSDTTtPIKLLA
T604-p	DVFSDTTtPIKLLAV
K639-u	NFENHSGkLGATAEK
K655-u	AAVGTANkSTVEGIQ
S664-p	TVEGIQAsVKTAREL
K666	EGIQAsVKTARELTP
Y692-p	NPGNQAAyEHFEtMk
T697-p	AAyEHFEtMkNQWID
K699-a	yEHFEtMkNQWIDNV
K699-u	yEHFEtMkNQWIDNV
T719	LVDEAIDTKsLLDAs
K720	VDEAIDTKsLLDAsE
S721-p	DEAIDTKsLLDAsEE
S726-p	TKsLLDAsEEAIKKD
K731	DAsEEAIKKDLDKCK
T754-p	QMLVAGAtSIARRAN
K768-a	NRILLVAkREVENSE
S774	AkREVENSEDPKFRE
K778	VENSEDPKFREAVKA
K784	PKFREAVKAASDELS
K784	PKFREAVKAASDELS
K792-u	AASDELSkTIsPMVM
S795-p	DELSkTIsPMVMDAk
K802	sPMVMDAKAVAGNIs
K802-u	sPMVMDAkAVAGNIs
S809-p	kAVAGNIsDPGLQks
K815-u	IsDPGLQksFLDsGy
S816-p	sDPGLQksFLDsGyR
S820-p	LQksFLDsGyRILGA
Y822-p	ksFLDsGyRILGAVA
K830	RILGAVAKVREAFQP
S913	HDEARKWSSKPGIPA
K983-u	EATKWSSkGNDIIAA
K992-a	NDIIAAAkrMALLMA
K992	NDIIAAAKrMALLMA
R993-m1	DIIAAAkrMALLMAE
R1003-m1	LLMAEMSrLVRGGSG
K1024	QCAKDIAKASDEVTR
K1064	PTISTQLKILSTVkA
K1070-a	LKILSTVkATMLGRT
K1070-u	LKILSTVkATMLGRT
S1101	NAQNLMQSVKETVRE
K1103	QNLMQSVKETVREAE
S1113	VREAEAASIKIRTDA
T1123-p	IRTDAGFtLRWVRKT
Y1133-p	WVRKTPWyQ______

	vinculin iso2
K59	SNLVRVGKETVQTTE
K71-a	TTEDQILkRDMPPAF
Y100	QMLQSDPYSVPARDY
S101	MLQSDPYSVPARDYL
Y107	YSVPARDYLIDGSRG
S112	RDYLIDGSRGILSGT
K173	PGMTKMAKMIDERQQ
K216	MKIFVTTKNSKNQGI
K219	FVTTKNSKNQGIEEA
K228	QGIEEALKNRNFTVE
S260	WDEDAWASKDTEAMK
K261	DEDAWASKDTEAMKR
S272	AMKRALASIDSKLNQ
S275	RALASIDSKLNQAKG
K276	ALASIDSKLNQAKGW
K281	DSKLNQAKGWLRDPS
S288	KGWLRDPSASPGDAG
S290	WLRDPSASPGDAGEQ
K308	QILDEAGKVGELCAG
K316	VGELCAGKERREILG
T324	ERREILGTCKMLGQM
S346	RARGQGSSPVAMQKA
S357	MQKAQQVSQGLDVLT
K366	GLDVLTAKVENAARK
K381	LEAMTNSKQSIAKKI
K386	NSKQSIAKKIDAAQN
K387	SKQSIAKKIDAAQNW
S434	ERDDILRSLGEISAL
K444	EISALTSKLADLRRQ
K464	PEARALAKQVATALQ
K476	ALQNLQTKTNRAVAN
K496	AAVHLEGKIEQAQRW
T508-p	QRWIDNPtVDDRGVG
S566	AARGEGESPQARALA
S579	LASQLQDSLKDLKAR
K581	SQLQDSLKDLKARMQ
K584	QDSLKDLKARMQEAM
S600	QEVSDVFSDTTTPIK
T603	SDVFSDTTTPIKLLA
T604	DVFSDTTTPIKLLAV
K639	NFENHSGKLGATAEK
K655	AAVGTANKSTVEGIQ
S664	TVEGIQASVKTAREL
K666	EGIQASVKTARELTP
Y692-p	NPGNQAAyEHFETMK
T697	AAyEHFETMKNQWID
K699	yEHFETMKNQWIDNV
K699	yEHFETMKNQWIDNV
T719	LVDEAIDTKSLLDAS
K720	VDEAIDTKSLLDASE
S721	DEAIDTKSLLDASEE
S726	TKSLLDASEEAIKKD
K731	DASEEAIKKDLDKCK
T754	QMLVAGATSIARRAN
K768-a	NRILLVAkREVENSE
S774	AkREVENSEDPKFRE
K778	VENSEDPKFREAVKA
K784	PKFREAVKAASDELS
K784	PKFREAVKAASDELS
K792	AASDELSKTISPMVM
S795	DELSKTISPMVMDAK
K802	SPMVMDAKAVAGNIS
K802	SPMVMDAKAVAGNIS
S809	KAVAGNISDPGLQKS
K815	ISDPGLQKSFLDSGy
S816	SDPGLQKSFLDSGyR
S820	LQKSFLDSGyRILGA
Y822-p	KSFLDSGyRILGAVA
K830	RILGAVAKVREAFQP
S913	HDEARKWSSKGNDII
K915	EARKWSSKGNDIIAA
K924-a	NDIIAAAkRMALLMA
K924	NDIIAAAKRMALLMA
R925	DIIAAAkRMALLMAE
R935	LLMAEMSRLVRGGSG
K956	QCAKDIAKASDEVTR
K996	PTISTQLKILSTVkA
K1002-a	LKILSTVkATMLGRT
K1002	LKILSTVKATMLGRT
S1033	NAQNLMQSVKETVRE
K1035	QNLMQSVKETVREAE
S1045	VREAEAASIKIRTDA
T1055	IRTDAGFTLRWVRKT
Y1065-p	WVRKTPWyQ______

	mouse
K59-u	SNLVRVGkETVQTTE
K71	TTEDQILKRDMPPAF
Y100-p	QMLQSDPySVPARDY
S101	MLQSDPySVPARDYL
Y107	ySVPARDYLIDGSRG
S112	RDYLIDGSRGILSGT
K173	PGMTKMAKMIDERQQ
K216-u	MKIFVTTkNSkNQGI
K219-u	FVTTkNSkNQGIEEA
K228-u	QGIEEALkNRNFTVE
S260-p	WDEDAWAskDTEAMK
K261-u	DEDAWAskDTEAMKR
S272-p	AMKRALAsIDSkLNQ
S275	RALAsIDSkLNQAkG
K276-u	ALAsIDSkLNQAkGW
K281-u	DSkLNQAkGWLRDPN
N288	kGWLRDPNAsPGDAG
S290-p	WLRDPNAsPGDAGEQ
K308-u	QILDEAGkVGELCAG
K316-a	VGELCAGkERREILG
T324-p	ERREILGtCKMLGQM
S346-p	RARGQGAsPVAMQKA
S357	MQKAQQVSQGLDVLT
K366-u	GLDVLTAkVENAARK
K381-u	LEAMTNSkQSIAKkI
K386	NSkQSIAKkIDAAQN
K387-u	SkQSIAKkIDAAQNW
S434-p	ERDDILRsLGEIAAL
K444-u	EIAALTSkLGDLRRQ
K464-u	PEARALAkQVATALQ
K476-u	ALQNLQTkTNRAVAN
K496	AAVHLEGKIEQAQRW
T508	QRWIDNPTVDDRGVG
S566	AARGEGESPQARALA
S579-p	LASQLQDsLkDLkAQ
K581-u	SQLQDsLkDLkAQMQ
K584-u	QDsLkDLkAQMQEAM
S600-p	QEVSDVFsDTttPIK
T603-p	SDVFsDTttPIKLLA
T604-p	DVFsDTttPIKLLAV
R639	NFENHSGRLGATAEK
K655-u	AAVGTANkSTVEGIQ
S664	TVEGIQASVkTAREL
K666-u	EGIQASVkTARELTP
Y692-p	NPGNQAAyEHFEtMk
T697-p	AAyEHFEtMkNQWID
K699	yEHFEtMKNQWIDNV
K699-u	yEHFEtMkNQWIDNV
T719-p	LVDEAIDtksLLDAs
K720-u	VDEAIDtksLLDAsE
S721-p	DEAIDtksLLDAsEE
S726-p	tksLLDAsEEAIkKD
K731-u	DAsEEAIkKDLDKCK
T754	QMLVAGATSIARRAN
K768	NRILLVAKREVENsE
S774-p	AKREVENsEDPkFRE
K778-u	VENsEDPkFREAVkA
K784-a	PkFREAVkAASDELS
K784-u	PkFREAVkAASDELS
K792-u	AASDELSkTIsPMVM
S795-p	DELSkTIsPMVMDAk
K802-a	sPMVMDAkAVAGNIs
K802-u	sPMVMDAkAVAGNIs
S809-p	kAVAGNIsDPGLQks
K815-u	IsDPGLQksFLDsGy
S816-p	sDPGLQksFLDsGyR
S820-p	LQksFLDsGyRILGA
Y822-p	ksFLDsGyRILGAVA
K830-u	RILGAVAkVREAFQP
S913-p	HDEARKWsSkGNDII
K915-u	EARKWsSkGNDIIAA
K924-a	NDIIAAAkRMALLMA
K924-u	NDIIAAAkRMALLMA
R925	DIIAAAkRMALLMAE
R935	LLMAEMSRLVRGGSG
K956-u	QCAKDIAkASDEVTR
K996-u	PTISTQLkILSTVkA
K1002-a	LkILSTVkATMLGRT
K1002-u	LkILSTVkATMLGRT
S1033	NAQNLMQSVkETVRE
K1035-u	QNLMQSVkETVREAE
S1045	VREAEAASIKIRTDA
T1055	IRTDAGFTLRWVRKT
Y1065-p	WVRKTPWyQ______

	chicken
K59	SNLVRVGKETVQTTE
K71	TTEDQILKRDMPPAF
Y100-p	QMLQADPySVPARDY
S101	MLQADPySVPARDYL
Y107	ySVPARDYLIDGSRG
S112	RDYLIDGSRGILSGT
K173	PGMTKMAKMIDERQQ
K216	MKIFVTTKNTKSQGI
K219	FVTTKNTKSQGIEEA
K228	QGIEEALKNRNFTVE
S260	WDEDAWASKDTEAMK
K261	DEDAWASKDTEAMKR
L272	AMKRALALIDSKMNQ
S275	RALALIDSKMNQAKG
K276	ALALIDSKMNQAKGW
K281	DSKMNQAKGWLRDPN
N288	KGWLRDPNAPPGDAG
P290	WLRDPNAPPGDAGEQ
K308	QILDEAGKAGELCAG
K316	AGELCAGKERREILG
T324	ERREILGTCKTLGQM
T346	RARGQGATPMAMQKA
S357	MQKAQQVSQGLDLLT
K366	GLDLLTAKVENAARK
K381	LEAMTNSKQAIAKKI
K386	NSKQAIAKKIDAAQN
K387	SKQAIAKKIDAAQNW
S434	ERDDILRSLGEISAL
K444	EISALTAKLSDLRRH
K464	PEARALAKQIATSLQ
K476	SLQNLQSKTNRAVAN
K496	AAVHLEGKIEQAQRW
T508	QRWIDNPTVDDRGVG
S566	AARGEGESPQARAIA
S579	IAAQLQDSLKDLKAR
K581	AQLQDSLKDLKARMQ
K584	QDSLKDLKARMQEAM
S600	QEVSDVFSDTTTPIK
T603	SDVFSDTTTPIKLLA
T604	DVFSDTTTPIKLLAV
R639	NFENHAARLGATAEK
K655	AAVGTANKTTVEGIQ
T664	TVEGIQATVKSAREL
K666	EGIQATVKSARELTP
Y692	NPGNQAAYEHFETMK
T697	AAYEHFETMKNQWID
K699	YEHFETMKNQWIDNV
K699	YEHFETMKNQWIDNV
T719	LVDEAIDTKSLLDAS
K720	VDEAIDTKSLLDASE
S721	DEAIDTKSLLDASEE
S726	TKSLLDASEEAIKKD
K731	DASEEAIKKDLDKCK
T754	QMLVAGATSIARRAN
K768	NRILLVAKREVENSE
S774	AKREVENSEDPKFRE
K778	VENSEDPKFREAVKA
K784	PKFREAVKAASDELS
K784	PKFREAVKAASDELS
K792	AASDELSKTISPMVM
S795	DELSKTISPMVMDAK
K802	SPMVMDAKAVAGNIS
K802	SPMVMDAKAVAGNIS
S809	KAVAGNISDPGLQKS
K815	ISDPGLQKSFLDSGY
S816	SDPGLQKSFLDSGYR
S820	LQKSFLDSGYRILGA
Y822	KSFLDSGYRILGAVA
K830	RILGAVAKVREAFQP
S913	HDEARKWSSKPVTVI
K984	EATKWSSKGNDIIAA
K993	NDIIAAAKRMALLMA
K993	NDIIAAAKRMALLMA
R994	DIIAAAKRMALLMAE
R1004	LLMAEMSRLVRGGSG
K1025	QCAKDIAKASDEVTR
K1065	PTISTQLKILSTVKA
K1071	LKILSTVKATMLGRT
K1071	LKILSTVKATMLGRT
S1102-p	NAQNLMQsVKETVRE
K1104	QNLMQsVKETVREAE
S1114-p	VREAEAAsIKIRTDA
T1124	IRTDAGFTLRWVRKT
Y1134-p	WVRKTPWyQ______

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