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Protein Page:
IRF3 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
IRF3 interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Note: This description may include information from UniProtKB.
Protein type: Transcription factor; DNA binding protein
Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; negative regulation of interferon-beta biosynthetic process; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of defense response to virus by host; response to exogenous dsRNA; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon-beta production; positive regulation of interferon type I production; innate immune response; interferon type I biosynthetic process; negative regulation of interferon type I production; defense response to virus; response to DNA damage stimulus; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; positive regulation of cytokine secretion
Reference #:  Q14653 (UniProtKB)
Alt. Names/Synonyms: Interferon regulatory factor 3; IRF-3; IRF3
Gene Symbols: IRF3
Molecular weight: 47,219 Da
Basal Isoelectric point: 5.17  Predict pI for various phosphorylation states
CST Pathways:  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

IRF3

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: S173‑p, S339‑p, S385‑p, S386‑p, S396‑p, S398‑p, S402‑p, T404‑p, S405‑p
activity, induced: S385‑p, S386‑p, S396‑p, S398‑p, S402‑p, T404‑p, S405‑p
intracellular localization: T135‑p, S385‑p, S386‑p, S396‑p, S398‑p, S402‑p, T404‑p, S405‑p
molecular association, regulation: S339‑p, S385‑p, S386‑p, T390‑p, S396‑p, S398‑p, T404‑p, S405‑p
phosphorylation: S173‑p, S386‑p, T390‑p, T404‑p, S405‑p
protein conformation: S385‑p, S386‑p, T404‑p, S405‑p
protein degradation: S339‑p, S396‑p
protein stabilization: T135‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S14-p RILPWLVsQLDLGQL
1 0 K70-s AYVPGRDkPDLPTWK
1 1 K70-u AYVPGRDkPDLPTWK
1 0 K87-s FRSALNRkEGLRLAE
1 0 K87-u FRSALNRkEGLRLAE
2 5 S123-p DFSQPDTsPDTNGGG
0 1 T126 QPDTsPDTNGGGSTS
0 2 S131 PDTNGGGSTSDtQED
2 19 T135-p GGGSTSDtQEDILDE
2 0 P153 NMVLAPLPDPGPPSL
5 2 S173-p PCPQPLRsPsLDNPt
2 4 S175-p PQPLRsPsLDNPtPF
2 0 T180-p sPsLDNPtPFPNLGP
1 0 S188-p PFPNLGPsENPLkRL
0 1 K193-u GPsENPLkRLLVPGE
2 0 S339-p FTEGSGRsPRYALWF
0 6 T370-p VMVKVVPtCLRALVE
0 1 R373 KVVPtCLRALVEMAR
7 1 S385-p MARVGGAssLENtVD
8 2 S386-p ARVGGAssLENtVDL
1 1 T390-p GAssLENtVDLHIsN
24 2 S396-p NtVDLHIsNsHPLsL
6 0 S398-p VDLHIsNsHPLsLts
5 1 S402-p IsNsHPLsLtsDQYK
5 0 T404-p NsHPLsLtsDQYKAY
4 0 S405-p sHPLsLtsDQYKAYL
4947 : Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb
  mouse

 
S14 RILPWLVSQLDLGQL
K70 AYTPGKDKPDVSTWK
K70 AYTPGKDKPDVSTWK
K87 FRSALNRKEVLRLAA
K87-u FRSALNRkEVLRLAA
S123-p DFVHLGAsPDtNGKS
T126-p HLGAsPDtNGKSsLP
S131-p PDtNGKSsLPHsQEN
S135-p GKSsLPHsQENLPKL
K152-s GLILGPLkDEGSSDL
S171-p DPSQQLPsPNVNNFL
N173 SQQLPsPNVNNFLNP
- gap
Q183 NFLNPAPQENPLKQL
K188 APQENPLKQLLAEEQ
S332 FMEGSGHSPRYTLWF
T363 VMVKVVPTCLkELLE
K366-u KVVPTCLkELLEMAR
S378 MAREGGASSLKTVDL
S379 AREGGASSLKTVDLH
T382 GGASSLKTVDLHIsN
S388-p KTVDLHIsNSQPISL
S390 VDLHIsNSQPISLtS
S394 IsNSQPISLtSDQYK
T396-p NSQPISLtSDQYKAY
S397 SQPISLtSDQYKAYL
  rat

 
S14 LILPWLVSQLDLGQL
K70 AYTPGKDKPDLSTWK
K70 AYTPGKDKPDLSTWK
K87 FRSALNRKEVLRLAE
K87 FRSALNRKEVLRLAE
S124 DFVHLDtSPDTNGKS
T127 HLDtSPDTNGKSSLS
S132 PDTNGKSSLSDHQED
H136 GKSSLSDHQEDLLEL
P153 HMALGPLPDEGSSDL
S172 DPSQPPLSPIVNNFP
I174 SQPPLSPIVNNFPNP
- gap
Q184 NFPNPAPQENPLRQL
R189 APQENPLRQLLAEEQ
S334 FMEGSRHSPRYTLWF
T365 VMVKVVPTCLKELLE
K368 KVVPTCLKELLEMAR
S380 MAREGGASSLKTVDL
S381 AREGGASSLKTVDLH
T384 GGASSLKTVDLHISN
S390 KTVDLHISNSQPISL
S392 VDLHISNSQPISLTS
S396 ISNSQPISLTSDQYK
T398 NSQPISLTSDQYKAC
S399 SQPISLTSDQYKACL
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