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Protein Page:
IRF3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
IRF3 interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: identical protein binding; protein binding; protein homodimerization activity; DNA binding; transcription cofactor activity; transcription factor activity
Biological Process: negative regulation of interferon-beta biosynthetic process; transcription from RNA polymerase II promoter; positive regulation of I-kappaB kinase/NF-kappaB cascade; viral reproduction; apoptosis; MyD88-independent toll-like receptor signaling pathway; cytokine and chemokine mediated signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of defense response to virus by host; toll-like receptor 3 signaling pathway; response to exogenous dsRNA; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; positive regulation of interferon type I production; positive regulation of interferon-beta production; innate immune response; interferon type I biosynthetic process; response to DNA damage stimulus; negative regulation of interferon type I production; toll-like receptor 4 signaling pathway; defense response to virus; positive regulation of interferon-alpha production; positive regulation of cytokine secretion
Reference #:  Q14653 (UniProtKB)
Alt. Names/Synonyms: Interferon regulatory factor 3; IRF-3; IRF3
Gene Symbols: IRF3
Molecular weight: 47,219 Da
Basal Isoelectric point: 5.17  Predict pI for various phosphorylation states
CST Pathways:  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

IRF3

Protein Structure Not Found.


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Sites Implicated In
transcription, altered: S173‑p, S339‑p, S385‑p, S386‑p, S396‑p, S398‑p, S402‑p, T404‑p, S405‑p
activity, induced: S385‑p, S386‑p, S396‑p, S398‑p, S402‑p, T404‑p, S405‑p
intracellular localization: T135‑p, S385‑p, S386‑p, S396‑p, S398‑p, S402‑p, T404‑p, S405‑p
molecular association, regulation: S339‑p, S385‑p, S386‑p, T390‑p, S396‑p, S398‑p, T404‑p, S405‑p
phosphorylation: S173‑p, S386‑p, T390‑p, T404‑p, S405‑p
protein conformation: S385‑p, S386‑p, T404‑p, S405‑p
protein degradation: S339‑p, S396‑p
protein stabilization: T135‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S14-p RILPWLVsQLDLGQL
0 1 K29-ub EGVAWVNkSRTRFRI
1 1 K70-ub AYVPGRDkPDLPTWK
1 0 K70-sm AYVPGRDkPDLPTWK
1 0 K87-ub FRSALNRkEGLRLAE
1 0 K87-sm FRSALNRkEGLRLAE
0 1 K105 KDPHDPHKIYEFVNS
2 6 S123-p DFSQPDTsPDTNGGG
0 1 T126 QPDTsPDTNGGGSTS
0 2 S131 PDTNGGGSTSDtQED
2 20 T135-p GGGSTSDtQEDILDE
2 0 P153 NMVLAPLPDPGPPSL
5 2 S173-p PCPQPLRsPsLDNPt
2 4 S175-p PQPLRsPsLDNPtPF
2 0 T180-p sPsLDNPtPFPNLGP
1 0 S188-p PFPNLGPsENPLkRL
0 1 K193-ub GPsENPLkRLLVPGE
2 0 S339-p FTEGSGRsPRYALWF
0 6 T370-p VMVKVVPtCLRALVE
0 1 R373 KVVPtCLRALVEMAR
7 1 S385-p MARVGGAssLENtVD
8 2 S386-p ARVGGAssLENtVDL
1 1 T390-p GAssLENtVDLHIsN
25 2 S396-p NtVDLHIsNsHPLsL
7 0 S398-p VDLHIsNsHPLsLts
6 1 S402-p IsNsHPLsLtsDQYK
6 0 T404-p NsHPLsLtsDQYKAY
5 0 S405-p sHPLsLtsDQYKAYL
4947 : Phospho-IRF-3 (Ser396) (4D4G) Rabbit mAb
  mouse

 
S14 RILPWLVSQLDLGQL
E29 EGVAWLDESRTRFRI
K70 AYTPGKDKPDVSTWK
K70 AYTPGKDKPDVSTWK
K87-ub FRSALNRkEVLRLAA
K87 FRSALNRKEVLRLAA
K105-ac KDPYDPHkVYEFVTP
S123-p DFVHLGAsPDtNGKS
T126-p HLGAsPDtNGKSsLP
S131-p PDtNGKSsLPHsQEN
S135-p GKSsLPHsQENLPKL
K152-sm GLILGPLkDEGSSDL
S171-p DPSQQLPsPNVNNFL
N173 SQQLPsPNVNNFLNP
- gap
Q183 NFLNPAPQENPLKQL
K188 APQENPLKQLLAEEQ
S332 FMEGSGHSPRYTLWF
T363 VMVKVVPTCLkELLE
K366-ub KVVPTCLkELLEMAR
S378 MAREGGASSLKTVDL
S379 AREGGASSLKTVDLH
T382 GGASSLKTVDLHIsN
S388-p KTVDLHIsNSQPISL
S390 VDLHIsNSQPISLtS
S394 IsNSQPISLtSDQYK
T396-p NSQPISLtSDQYKAY
S397 SQPISLtSDQYKAYL
  rat

 
S14 LILPWLVSQLDLGQL
E29 KGVAWLDESRTKFRI
K70 AYTPGKDKPDLSTWK
K70 AYTPGKDKPDLSTWK
K87 FRSALNRKEVLRLAE
K87 FRSALNRKEVLRLAE
K105 KDPFDPHKVYEFVTP
S124 DFVHLDtSPDTNGKS
T127 HLDtSPDTNGKSSLS
S132 PDTNGKSSLSDHQED
H136 GKSSLSDHQEDLLEL
P153 HMALGPLPDEGSSDL
S172 DPSQPPLSPIVNNFP
I174 SQPPLSPIVNNFPNP
- gap
Q184 NFPNPAPQENPLRQL
R189 APQENPLRQLLAEEQ
S334 FMEGSRHSPRYTLWF
T365 VMVKVVPTCLKELLE
K368 KVVPTCLKELLEMAR
S380 MAREGGASSLKTVDL
S381 AREGGASSLKTVDLH
T384 GGASSLKTVDLHISN
S390 KTVDLHISNSQPISL
S392 VDLHISNSQPISLTS
S396 ISNSQPISLTSDQYK
T398 NSQPISLTSDQYKAC
S399 SQPISLTSDQYKACL
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