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Protein Page:
NOTCH4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NOTCH4 Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May regulate branching morphogenesis in the developing vascular system. Heterodimer of a C-terminal fragment N(TM) and a N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH4. Highly expressed in the heart, moderately in the lung and placenta and at low levels in the liver, skeletal muscle, kidney, pancreas, spleen, lymph node, thymus, bone marrow and fetal liver. No expression was seen in adult brain or peripheral blood leukocytes. Belongs to the NOTCH family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p21.3
Cellular Component: nucleoplasm; Golgi membrane; endoplasmic reticulum membrane; cell surface; integral to plasma membrane; extracellular region; plasma membrane; cytosol; nucleus
Molecular Function: protein binding; protein heterodimerization activity; calcium ion binding; receptor activity
Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; positive regulation of transcription, DNA-dependent; Notch receptor processing; morphogenesis of a branching structure; cell fate determination; activation of Notch receptor target transcription factor; patterning of blood vessels; negative regulation of cell differentiation; embryonic development; negative regulation of endothelial cell differentiation; mammary gland development; gene expression; hemopoiesis; endothelial cell morphogenesis; cell differentiation
Reference #:  Q99466 (UniProtKB)
Alt. Names/Synonyms: FLJ16302; hNotch4; INT3; MGC74442; Neurogenic locus notch homolog protein 4; NOTC4; Notch 4; Notch 4 extracellular truncation; Notch 4 intracellular domain; Notch homolog 4 (Drosophila); NOTCH3; NOTCH4
Gene Symbols: NOTCH4
Molecular weight: 209,622 Da
Basal Isoelectric point: 5.43  Predict pI for various phosphorylation states
CST Pathways:  ESC Pluripotency and Differentiation  |  Notch Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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NOTCH4

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 S125-p LEDPCPPsFCsKRGR
0 1 S128-p PCPPsFCsKRGRCHI
0 1 S378-p IDRVGSFsCLCPPGR
0 1 S1118-p PPRCACLsGYGGPDC
0 1 S1508-p RPPLGEDsIGLKALK
0 1 K1735 GARDKWGKTALHWAA
0 1 - gap
0 1 R1871 LSAGAGPRGGGACLQ
  mouse

 
S122 LEELCPPSFCSNGGH
S125 LCPPSFCSNGGHCYV
S375 IDRVGSFSCLCPPGR
S1114 PPLCACLSGFGGPDC
N1504 RPPLGEDNIGLKALK
K1730-ac GARDKRGkTALHWAA
T1830-p EARAHARtTPGGGAA
R1850-m2 LSAGARPrGGGACLQ
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