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Protein Page:
PIWIL1 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PIWIL1 Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer- independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability. Isoform 3 may be a negative developmental regulator. Interacts (via Piwi domain) with DICER1, suggesting that it forms ribonucleoprotein RISC complexes. This interaction is regulated by HSP90AB1 activity. Interacts with MAEL, KIF17, PABPC1, PRMT5 and WDR77. Interacts (when methylated on arginine residues) with TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9. Isoform 3 is down-regulated in CD34(+) hematopoietic cells during differentiation. Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells. Belongs to the argonaute family. Piwi subfamily. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Membrane protein, peripheral
Cellular Component: polysome; mRNA cap complex; cytoplasm
Molecular Function: mRNA binding; protein binding; single-stranded RNA binding; nucleic acid binding; RNA binding; protein kinase binding
Biological Process: regulation of translation; meiosis; multicellular organismal development; RNA-mediated gene silencing; spermatogenesis; cell differentiation; spermatid development
Reference #:  Q9JMB7 (UniProtKB)
Alt. Names/Synonyms: MGC150073; Miwi; piwi like homolog 1; piwi-like homolog 1 (Drosophila); Piwi-like protein 1; Piwil1; PIWL1
Gene Symbols: Piwil1
Molecular weight: 98,574 Da
Basal Isoelectric point: 9.46  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PIWIL1

Protein Structure Not Found.


STRING  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
1 0 R14-m1 ARARGRArGQETVQH
1 0 R14-m2 ARARGRArGQETVQH
1 0 R49-m1 TEGDLVGrGRQRGMV
0 1 S108 VKESKTGSSGIIVKL
0 1 T123 STNHFRLTSRPQWAL
0 1 S124 TNHFRLTSRPQWALY
1 0 R371-m1 VSQPKRRrGPGGTLP
0 1 K405-ub RNDFNVMkDLAVHTR
0 1 K431-ub RLIDYIHkDDNVQRE
0 1 K462-ub GRILQSEkIHQGGKT
0 1 K481-ub PQFADWSkETRGAPL
0 1 K574-ub DKYDAIKkYLCTDCP
0 1 K604 TVMAIATKIALQMNC
0 1 S644 DTTAGRRSIAGFVAs
0 3 S651-p SIAGFVAsINEGMTR
0 3 T737-p RGYNPRLtVIVVKKR
0 1 Y861 SLSNRLYYL______
  human

 
R14 ARARGRARGQETAQL
R14 ARARGRARGQETAQL
R49 AEGELFGRGRQRGTA
S107-p VKESKTGsSGIIVRL
T122-p STNHFRLtsRPQWAL
S123-p TNHFRLtsRPQWALY
R370 VSQPKRRRGPGGTLP
K404 RNDFNVMKDLAVHTR
K430 RLIDYIHKNDNVQRE
K461 GRILQTEKIHQGGKT
K480 PQFADWSKETRGAPL
K573 DKYDAIKKYLCTDCP
K603-ub TVMAIATkIALQMNC
S643-p DMTAGRRsIAGFVAs
S650-p sIAGFVAsINEGMTR
T736-p RGYNPRLtVIVVKKR
Y860-p SLSNRLYyL______
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