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Protein Page:
KLHL12 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
KLHL12 Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport. The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division: BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3. The BCR(KLHL12) complex also mediates polyubiquitination of DRD4, without leading to degradation of DRD4. Component of the BCR(KLHL12) E3 ubiquitin ligase complex, at least composed of CUL3 and KLHL12 and RBX1. This complex interacts with DVL3 upon activation of the Wnt signaling pathway by WNT3A. Interacts with DRD4, KLHL12 and SEC31A. Ubiquitously expressed. Highly expressed in testis and at lower levels in the submandibular salivary gland. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin conjugating system
Cellular Component: Golgi membrane; ER to Golgi transport vesicle
Molecular Function: protein binding
Biological Process: COPII coating of Golgi vesicle; protein monoubiquitination; ER to Golgi vesicle-mediated transport; Wnt receptor signaling pathway
Reference #:  Q53G59 (UniProtKB)
Alt. Names/Synonyms: C3IP1; CUL3-interacting protein 1; DKIR; FLJ27152; kelch-like 12 (Drosophila); Kelch-like protein 12; kelch-like protein C3IP1; KLH12; KLHL12
Gene Symbols: KLHL12
Molecular weight: 63,277 Da
Basal Isoelectric point: 5.26  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

KLHL12

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T31-p NSLRKSNtLCDVtLR
0 1 T36-p SNtLCDVtLRVEQKD
0 2 S175-p HEEFILLsQGEVEKL
0 1 Y231-p MPLLTPRyITDVIDA
0 1 K256-ub RDLVDEAkKFHLRPE
0 1 K301-ub SPIDVVEkYDPkTQE
0 1 K305-ub VVEkYDPkTQEWSFL
0 1 R320 PSITRKRRYVASVSL
  mouse

 
T31 NSLRKSNTLCDVTLR
T36 SNTLCDVTLRVEQKD
S175 HEEFILLSQGEVEKL
Y231 MPLLTPRYITDVIDA
K256 RDLVDEAKKFHLRPE
K301 SPIDVVEKYDPKTQE
K305 VVEKYDPKTQEWSFL
R320-m2 PSITRKRrYVASVSL
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