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Protein Page:
Ack (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
Ack a tyrosine kinase that binds Cdc42 in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. May be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42 and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Amplification in primary tumors correlates with metastatic potential . Two differentially spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Protein kinase, TK; EC 2.7.10.2; Kinase, protein; Protein kinase, tyrosine (non-receptor); TK group; Ack family
Cellular Component: cytoplasmic vesicle membrane; growth cone; adherens junction; cell soma; clathrin-coated vesicle; axon; dendrite; cytoplasm; plasma membrane; coated pit; nucleus; endosome
Molecular Function: protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; metal ion binding; WW domain binding; protein serine/threonine/tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; GTPase inhibitor activity; ATP binding; epidermal growth factor receptor binding
Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; small GTPase mediated signal transduction; negative regulation of catalytic activity; endocytosis; phosphorylation
Reference #:  Q07912 (UniProtKB)
Alt. Names/Synonyms: ACK; ACK-1; ACK1; Activated CDC42 kinase 1; activated Cdc42-associated kinase 1; activated p21cdc42Hs kinase; FLJ44758; FLJ45547; p21cdc42Hs; TNK2; Tyrosine kinase non-receptor protein 2; tyrosine kinase, non-receptor, 2
Gene Symbols: TNK2
Molecular weight: 114,569 Da
Basal Isoelectric point: 6.84  Predict pI for various phosphorylation states
CST Pathways:  Tyrosine Kinases & Substrates
Select Structure to View Below

Ack

Protein Structure Not Found.


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Sites Implicated In
enzymatic activity, induced: Y284‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 - gap
0 1 - gap
0 1 T33 LRDDLNVTRLsHFEY
0 1 S36-p DLNVTRLsHFEYVKN
0 7 Y40 TRLsHFEYVKNEDLE
0 1 S74-p KALCKRKsWMSKVFS
0 1 T101-p SQSTFRKtSPAPGGP
0 4 P113 GGPAGEGPLQSLTCL
0 18 K131-ub KDLRLLEkLGDGsFG
0 1 S136-p LEkLGDGsFGVVRRG
0 1 S149-p RGEWDAPsGKTVSVA
0 1 S183 REVNAMHSLDHRNLI
0 2 Y193-p HRNLIRLyGVVLTPP
0 1 T205-p TPPMKMVtELAPLGs
0 1 S212-p tELAPLGsLLDRLRK
5 226 Y284-p LPQNDDHyVMQEHRK
0 1 T465-p LQNSFIHtGHGDSDP
0 1 - gap
0 13 T517-p LGGVKKPtyDPVSED
0 747 Y518-p GGVKKPtyDPVSEDQ
0 5 S522 KPtyDPVSEDQDPLS
0 1 K550 PRGLWLAKPSARVPG
0 2 T603-p ACSLLDEtPPQsPTR
0 2 S607-p LDEtPPQsPTRALPR
0 5 Y635-p PLPPPPAyDDVAQDE
0 4 S724-p QLQAPAGsPAPsPsP
0 3 S728-p PAGsPAPsPsPGGDD
0 2 S730-p GsPAPsPsPGGDDKP
0 8 S757 TRPHVQLSPAPPGEE
0 4 S774 SQWPGPASPPRVPPR
0 4 S785-p VPPREPLsPQGSRTP
0 2 S810-p LPPRLSSsPGKTMPT
0 1 T818-p PGKTMPTtQSFASDP
0 1 K826-ac QSFASDPkyATPQVI
1 58 Y827-p SFASDPkyATPQVIQ
0 1 T829 ASDPkyATPQVIQAP
0 1 R839 VIQAPGPRAGPCILP
0 4 S855-p VRDGKKVsstHyyLL
0 22 S856-p RDGKKVsstHyyLLP
0 25 T857-p DGKKVsstHyyLLPE
1 553 Y859-p KKVsstHyyLLPERP
1 209 Y860-p KVsstHyyLLPERPs
0 3 S867-p yLLPERPsyLERyQR
0 4 Y868-p LLPERPsyLERyQRF
0 4 Y872-p RPsyLERyQRFLREA
0 2 S881-p RFLREAQsPEEPTPL
0 1 T925-p DPKANFStNNSNPGA
3138 : Phospho-Ack1 (Tyr284) Antibody
3097 : Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb
3137 : Phospho-Ack1 (Tyr857/858) Antibody
3097 : Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb
3137 : Phospho-Ack1 (Tyr857/858) Antibody
  Ack iso3  
S29-p GLRALLLsLTAAAGI
S39-p AAAGIWGsMGERSAY
T96 LRDDLNVTRLSHFEY
S99 DLNVTRLSHFEYVKN
Y103 TRLSHFEYVKNEDLE
S137 KALCKRKSWMSKVFS
T164 SQSTFRKTSPAPGGP
P176 GGPAGEGPLQSLTCL
K194 KDLRLLEKLGDGSFG
S199 LEKLGDGSFGVVRRG
S212 RGEWDAPSGKTVSVA
S246 REVNAMHSLDHRNLI
Y256 HRNLIRLYGVVLTPP
T268 TPPMKMVTELAPLGS
S275 TELAPLGSLLDRLRK
Y347 LPQNDDHYVMQEHRK
T528 LQNSFIHTGHGDSDP
T591 PPQPAFFTQKPTYDP
T595 AFFTQKPTYDPVSED
Y596 FFTQKPTYDPVSEDQ
S600 KPTYDPVSEDQDPLS
K628 PRGLWLAKPSARVPG
T681 ACSLLDETPPQSPTR
S685 LDETPPQSPTRALPR
Y713 PLPPPPAYDDVAQDE
S802 QLQAPAGSPAPSPSP
S806 PAGSPAPSPSPGGDD
S808 GSPAPSPSPGGDDKP
S835 TRPHVQLSPAPPGEE
S852 SQWPGPASPPRVPPR
S863 VPPREPLSPQGSRTP
S888 LPPRLSSSPGKTMPT
T896 PGKTMPTTQSFASDP
K904 QSFASDPKYATPQVI
Y905 SFASDPKYATPQVIQ
T907 ASDPKYATPQVIQAP
R917 VIQAPGPRAGPCILP
S933 VRDGKKVSSTHYYLL
S934 RDGKKVSSTHYYLLP
T935 DGKKVSSTHYYLLPE
Y937 KKVSSTHYYLLPERP
Y938 KVSSTHYYLLPERPS
S945 YLLPERPSYLERYQR
Y946 LLPERPSYLERYQRF
Y950 RPSYLERYQRFLREA
S959 RFLREAQSPEEPTPL
T1003 DPKANFSTNNSNPGA
  mouse

► Hide Isoforms
 
- gap
- gap
T33-p LRDDLNItRLSHFEy
S36 DLNItRLSHFEyVKN
Y40-p tRLSHFEyVKNEDLE
S74 KAMCKRKSWMSKVFS
P101 SQSTFRKPSPTPGSL
T113-p GSLPGEGtLQSLTCL
K131-ub KDLRLLEkLGDGSFG
S136 LEkLGDGSFGVVRRG
A149 RGEWDAPAGKTVSVA
S183 REVNAMHSLDHRNLI
Y193 HRNLIRLYGVVLTLP
T205 TLPMKMVTELAPLGS
S212 TELAPLGSLLDRLRK
Y284-p LPQNDDHyVMQEHRK
T465 LQNSFIHTGHGDSDP
T528-p PPQPAIFtQKTtyDP
T532-p AIFtQKTtyDPVSED
Y533-p IFtQKTtyDPVSEDP
S537 KTtyDPVSEDPDPLS
K565-ub PRGLWLAkPSARVPG
T618-p ACSLLDKtPPQsPTR
S622-p LDKtPPQsPTRALPR
Y650-p PLPPPPAyDDVAQDE
Q739 QLQVPTGQLTPsPTP
S743-p PTGQLTPsPTPGGDD
T745 GQLTPsPTPGGDDKP
S772-p TRPRVELsPAPSGEE
S789-p SRWPGPAsPPRVPPR
S800 VPPREPLSPQGSRTP
S825 LPHRLSSSPGKTMPT
T833 PGKTMPTTQSFASDP
K841 QSFASDPKyAtPQVI
Y842-p SFASDPKyAtPQVIQ
T844-p ASDPKyAtPQVIQAP
R854-m1 VIQAPGPrAGPCILP
S870-p VRDGRKVssTHyyLL
S871-p RDGRKVssTHyyLLP
T872 DGRKVssTHyyLLPE
Y874-p RKVssTHyyLLPERP
Y875-p KVssTHyyLLPERPP
P882 yLLPERPPYLERYQR
Y883 LLPERPPYLERYQRF
Y887 RPPYLERYQRFLREA
S896-p RFLREAQsPEEPAAL
T940 DPKANFSTNNSNPGA
3138 : Phospho-Ack1 (Tyr284) Antibody
3097 : Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb
3137 : Phospho-Ack1 (Tyr857/858) Antibody
3097 : Phospho-Ack1 (Tyr857/858) (C57E10) Rabbit mAb
3137 : Phospho-Ack1 (Tyr857/858) Antibody
  Ack iso2  
- gap
- gap
T33 LRDDLNITRLSHFEY
S36 DLNITRLSHFEYVKN
Y40 TRLSHFEYVKNEDLE
S74 KAMCKRKSWMSKVFS
P101 SQSTFRKPSPTPGSL
T113-p GSLPGEGtLQSLTCL
K131 KDLRLLEKLGDGSFG
S136 LEKLGDGSFGVVRRG
A149 RGEWDAPAGKTVSVA
S183 REVNAMHSLDHRNLI
Y193 HRNLIRLYGVVLTLP
T205 TLPMKMVTELAPLGS
S212 TELAPLGSLLDRLRK
Y284 LPQNDDHYVMQEHRK
T465 LQNSFIHTGHGDSDP
- gap
T517-p LGRVKKPtyDPVsED
Y518-p GRVKKPtyDPVsEDP
S522-p KPtyDPVsEDPDPLS
K550 PRGLWLAKPSARVPG
T603 ACSLLDKTPPQSPTR
S607 LDKTPPQSPTRALPR
Y635 PLPPPPAYDDVAQDE
Q724 QLQVPTGQLTPsPTP
S728-p PTGQLTPsPTPGGDD
T730 GQLTPsPTPGGDDKP
S757 TRPRVELSPAPSGEE
S774 SRWPGPASPPRVPPR
S785 VPPREPLSPQGSRTP
S810 LPHRLSSSPGKTMPT
T818 PGKTMPTTQSFASDP
K826 QSFASDPKYATPQVI
Y827 SFASDPKYATPQVIQ
T829 ASDPKYATPQVIQAP
R839 VIQAPGPRAGPCILP
S855 VRDGRKVSSTHyYLL
S856 RDGRKVSSTHyYLLP
T857 DGRKVSSTHyYLLPE
Y859-p RKVSSTHyYLLPERP
Y860 KVSSTHyYLLPERPP
P867 YLLPERPPYLERYQR
Y868 LLPERPPYLERYQRF
Y872 RPPYLERYQRFLREA
S881 RFLREAQSPEEPAAL
T925 DPKANFSTNNSNPGA
  rat

 
- gap
- gap
T33 LRDDLNITRLSHFEY
S36 DLNITRLSHFEYVKN
Y40 TRLSHFEYVKNEDLE
S74 KAMCKRKSWMSKVFS
P101 SQSTFRKPSPTPGGL
T113 GGLAGEGTLQSLTCL
K131 KDLRLLEKLGDGSFG
S136 LEKLGDGSFGVVRRG
A149 RGEWDAPAGKTVSVA
S183-p REVNAMHsLDHRNLI
Y193 HRNLIRLYGVVLTPP
T205 TPPMKMVTELAPLGS
S212 TELAPLGSLLDRLRK
Y284-p LPQNDGHyVMQEHRK
T465 LQNSFIHTGHGDSDP
- gap
T517 LGRMKKPTyDPVSED
Y518-p GRMKKPTyDPVSEDP
S522 KPTyDPVSEDPDPLS
K550 PRGLWLAKPSARVPG
T603 ACSLLDKTPPQSPTR
S607 LDKTPPQSPTRALPR
Y635 PLPPPPAYDDVAQDE
Q724 QLQVPTGQLTPSPTP
S728 PTGQLTPSPTPGGDD
T730 GQLTPSPTPGGDDKP
S757-p TRPRVGLsPAPLGEE
S774 SRWPGPSSPPRVPPR
S785 VPPREPLSPQGSRTP
S810 LPHRLSSSPGKTMPT
T818 PGKTMPTTQSFASDP
K826 QSFASDPKYATPQVI
Y827 SFASDPKYATPQVIQ
T829 ASDPKYATPQVIQAP
R839 VIQAPGPRAGPCILP
S855 VRDGRKVSSTHyyLL
S856 RDGRKVSSTHyyLLP
T857 DGRKVSSTHyyLLPE
Y859-p RKVSSTHyyLLPERP
Y860-p KVSSTHyyLLPERPP
P867 yLLPERPPYLERYQR
Y868 LLPERPPYLERYQRF
Y872 RPPYLERYQRFLREA
S881 RFLREAQSPEEPAAL
T925 DPKANFSTNNSNPGA
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