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Protein Page:
MCM4 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
MCM4 a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. The MCM hexameric protein complex is a key component of the pre-replication complex. The complex consisting of this protein and MCM2, 6 and 7 possesses DNA helicase activity. Phosphorylation by CDC2 reduces the DNA helicase activity and chromatin binding of the complex. Note: This description may include information from UniProtKB.
Protein type: DNA replication; EC 3.6.4.12
Cellular Component: nucleoplasm; membrane; MCM complex; nucleolus; nucleus
Molecular Function: ATP-dependent DNA helicase activity; protein binding; ATP binding; single-stranded DNA binding
Biological Process: DNA unwinding during replication; DNA replication initiation; DNA strand elongation during DNA replication; mitotic cell cycle; DNA replication; G1/S transition of mitotic cell cycle
Reference #:  P33991 (UniProtKB)
Alt. Names/Synonyms: CDC21; CDC21 homolog; CDC54; DNA replication licensing factor MCM4; hCdc21; homolog of S. pombe cell devision cycle 21; MCM4; MCM4 minichromosome maintenance deficient 4; MGC33310; minichromosome maintenance complex component 4; minichromosome maintenance deficient 4; P1-CDC21
Gene Symbols: MCM4
Molecular weight: 96,558 Da
Basal Isoelectric point: 6.28  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MCM4

Protein Structure Not Found.


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Sites Implicated In
activity, inhibited: T7‑p, T19‑p, S32‑p, S54‑p, T110‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 2 S2-p ______MssPAStPs
2 1 S3-p _____MssPAStPsR
2 0 T7-p _MssPAStPsRRGsR
0 1 S9-p ssPAStPsRRGsRRG
0 2 S13-p StPsRRGsRRGRAtP
3 20 T19-p GsRRGRAtPAQtPRs
0 1 A21 RRGRAtPAQtPRsED
0 17 T23-p GRAtPAQtPRsEDAR
0 12 S26-p tPAQtPRsEDARssP
0 14 S31-p PRsEDARssPsQRRR
2 14 S32-p RsEDARssPsQRRRG
0 6 S34-p EDARssPsQRRRGED
1 1 T53-p GELQPMPtsPGVDLQ
4 9 S54-p ELQPMPtsPGVDLQs
0 6 S61-p sPGVDLQsPAAQDVL
0 1 S71-p AQDVLFSsPPQMHSs
0 1 S78-p sPPQMHSsAIPLDFD
0 8 S87-p IPLDFDVssPLtyGt
1 12 S88-p PLDFDVssPLtyGtP
0 2 T91-p FDVssPLtyGtPSSR
0 6 Y92-p DVssPLtyGtPSSRV
0 1 T94-p ssPLtyGtPSSRVEG
0 12 T102-p PSSRVEGtPRsGVRG
0 17 S105-p RVEGtPRsGVRGtPV
2 16 T110-p PRsGVRGtPVRQRPD
0 14 S120-p RQRPDLGsAQkGLQV
0 1 K123-ac PDLGsAQkGLQVDLQ
0 15 S131-p GLQVDLQsdGAAAED
0 1 D132-ca LQVDLQsdGAAAEDI
0 3 S142-p AAEDIVAsEQsLGQK
0 4 S145-p DIVAsEQsLGQKLVI
0 14 K179-ub RFIDPLAkEEENVGI
0 4 K216-ub NVNCEHIkSFDkNLY
0 1 K220-ac EHIkSFDkNLYRQLI
0 8 K220-ub EHIkSFDkNLYRQLI
0 1 T236-p YPQEVIPtFDMAVNE
0 5 S326-p RGRIAEPsVCGRCHT
0 1 Y394-p RVNVTGIyRAVPIRV
0 2 S406-p IRVNPRVsNVkSVYK
0 1 K409-sc NPRVsNVkSVYKTHI
0 1 K439-ac LDEEAEQkLFSEKRV
0 1 K439-ub LDEEAEQkLFSEKRV
0 1 K450-ac EKRVELLkELsRkPD
0 6 K450-ub EKRVELLkELsRkPD
0 1 S453-p VELLkELsRkPDIyE
0 1 K455-ub LLkELsRkPDIyERL
0 2 Y459-p LsRkPDIyERLASAL
0 1 S464 DIyERLASALAPSIY
0 1 S469 LASALAPSIYEHEDI
0 1 Y471 SALAPSIYEHEDIkK
0 4 K477-ub IYEHEDIkKGILLQL
0 1 K477-sc IYEHEDIkKGILLQL
0 1 T488-p LLQLFGGtRKDFSHT
0 1 Y522-p SKSQLLQyVyNLVPR
0 1 Y524-p SQLLQyVyNLVPRGQ
0 4 K536-ub RGQYTSGkGSSAVGL
0 2 K549-ub GLTAYVMkDPETRQL
0 1 K578-ub CCIDEFDkMNESTRS
0 21 K600-ub QQTLSIAkAGIICQL
0 7 K627-ac IESQWNPkkTTIENI
0 2 K627-ub IESQWNPkkTTIENI
0 2 K628-ub ESQWNPkkTTIENIQ
0 1 S707-p PRLSEEAsQALIEAy
0 1 Y714-p sQALIEAyVDMRKIG
0 11 K746-ub RLAEAHAkVRLSNkV
0 5 K752-ub AkVRLSNkVEAIDVE
0 1 K762-ub AIDVEEAkRLHREAL
0 1 K762-sc AIDVEEAkRLHREAL
0 1 K770-ub RLHREALkQSATDPR
0 1 S784-p RTGIVDIsILTtGMS
0 1 T788-p VDIsILTtGMSATSR
0 1 K806-m3 EELAEALkKLILSKG
0 1 K814-ub KLILSKGkTPALkYQ
0 3 K819-ac KGkTPALkYQQLFED
0 21 K819-ub KGkTPALkYQQLFED
0 1 K837-ub QSDIAITkDMFEEAL
0 1 K858-ac DFLTVTGkTVRLL__
0 2 K858-ub DFLTVTGkTVRLL__
  mouse

 
S2-p ______MssPAStPS
S3-p _____MssPAStPSR
T7-p _MssPAStPSRRSSR
S9 ssPAStPSRRSSRRG
S13 StPSRRSSRRGRVtP
T19-p SSRRGRVtPtQsLRs
T21-p RRGRVtPtQsLRsEE
S23-p GRVtPtQsLRsEESR
S26-p tPtQsLRsEESRSsP
S31 LRsEESRSsPNRRRR
S32-p RsEESRSsPNRRRRG
N34 EESRSsPNRRRRGED
T52 GELLPMPTsPGADLQ
S53-p ELLPMPTsPGADLQS
S60 sPGADLQSPPAQNAL
S70 AQNALFSSPPQMHSL
L77 SPPQMHSLAIPLDFD
S86 IPLDFDVSsPLTYGT
S87-p PLDFDVSsPLTYGTP
T90 FDVSsPLTYGTPSSR
Y91 DVSsPLTYGTPSSRV
T93 SsPLTYGTPSSRVEG
T101-p PSSRVEGtPRsGVRG
S104-p RVEGtPRsGVRGtPV
T109-p PRsGVRGtPVRQRPD
S119-p RQRPDLGsARKGLQV
K122 PDLGsARKGLQVDLQ
S130-p GLQVDLQsDGAAAED
D131 LQVDLQsDGAAAEDI
S141 AAEDIVPSEQSLGQK
S144 DIVPSEQSLGQKLVI
K178 CFTDPLAKEEENVGI
K215 NVNCEHIKSFSKNLY
K219 EHIKSFSKNLYRQLI
K219 EHIKSFSKNLYRQLI
T235 YPQEVIPTFDMAVNE
C325 RGRIAEPCSCVHCHT
Y393 RVNVTGIYRAVPIRV
S405 IRVNPRVSNVKSVYK
K408 NPRVSNVKSVYKTHI
K438 LDEEAEQKLFSEKRV
K438 LDEEAEQKLFSEKRV
K449 EKRVKLLKELSRKPD
K449 EKRVKLLKELSRKPD
S452 VKLLKELSRKPDIyE
K454 LLKELSRKPDIyERL
Y458-p LSRKPDIyERLAsAL
S463-p DIyERLAsALAPsIy
S468-p LAsALAPsIyEHEDI
Y470-p sALAPsIyEHEDIKK
K476 IyEHEDIKKGILLQL
K476 IyEHEDIKKGILLQL
T487 LLQLFGGTRKDFSHT
Y521 SKSQLLQYVYNLVPR
Y523 SQLLQYVYNLVPRGQ
K535-ub RGQYTSGkGSSAVGL
K548 GLTAYVMKDPETRQL
K577 CCIDEFDKMNESTRS
K599 QQTLSIAKAGIICQL
K626 IESQWNPKKTTIENI
K626 IESQWNPKKTTIENI
K627 ESQWNPKKTTIENIQ
S706 PRLSEEASQALIEAY
Y713 SQALIEAYVNMRKIG
K745 RLAEAHAKVRFSNKV
K751 AKVRFSNKVEAIDVE
K761 AIDVEEAKRLHREAL
K761 AIDVEEAKRLHREAL
K769 RLHREALKQSATDPR
S783 RTGIVDISILTTGMS
T787 VDISILTTGMSATSR
R805 EELAEALRKLILSKG
K813 KLILSKGKTPALkYQ
K818 KGKTPALKYQQLFED
K818-ub KGKTPALkYQQLFED
K836 QSDTAITKDMFEEAL
K857 DFLTVTGKTVRLL__
K857 DFLTVTGKTVRLL__
  rat

 
S2 ______MSSPASTPS
S3 _____MSSPASTPSR
T7 _MSSPASTPSRRSSR
S9 SSPASTPSRRSSRRG
S13 STPSRRSSRRGRVtP
T19-p SSRRGRVtPTQSLRS
T21 RRGRVtPTQSLRSEE
S23 GRVtPTQSLRSEESR
S26 tPTQSLRSEESRSSP
S31 LRSEESRSSPSGRRR
S32 RSEESRSSPSGRRRG
S34 EESRSSPSGRRRGED
T52 GELLPMPTSPGADLQ
S53 ELLPMPTSPGADLQS
S60 SPGADLQSPAAQNAL
S70 AQNALFSSPPQMHSS
S77 SPPQMHSSAIPLEFD
S86 IPLEFDVSSPLTYGT
S87 PLEFDVSSPLTYGTP
T90 FDVSSPLTYGTPSSR
Y91 DVSSPLTYGTPSSRV
T93 SSPLTYGTPSSRVEG
T101 PSSRVEGTPRSGVRG
S104 RVEGTPRSGVRGTPM
T109 PRSGVRGTPMRQRPD
S119 RQRPDLGSARKGLQV
K122 PDLGSARKGLQVDLQ
S130 GLQVDLQSDGAAAED
D131 LQVDLQSDGAAAEDT
S141 AAEDTVASEQSLGQK
S144 DTVASEQSLGQKLVI
K178 CFTDPLAKEEENVGI
K215 NVNCEHIKSFGKNLY
K219 EHIKSFGKNLYRQLI
K219 EHIKSFGKNLYRQLI
T235 YPQEVIPTFDMAVNE
C325 RGRIAEPCTCVHCHT
Y393 RVNVTGIYRAVPIRV
S405 IRVNPRVSNVKSVYK
K408 NPRVSNVKSVYKTHI
K438 LDEEAEQKLFSEKRV
K438 LDEEAEQKLFSEKRV
K449 EKRVKLLKELSRKPD
K449 EKRVKLLKELSRKPD
S452 VKLLKELSRKPDIYE
K454 LLKELSRKPDIYERL
Y458 LSRKPDIYERLASAL
S463 DIYERLASALAPSIY
S468 LASALAPSIYEHEDI
Y470 SALAPSIYEHEDIKK
K476 IYEHEDIKKGILLQL
K476 IYEHEDIKKGILLQL
T487 LLQLFGGTRKDFSHT
Y521 SKSQLLQYVYNLVPR
Y523 SQLLQYVYNLVPRGQ
K535 RGQYTSGKGSSAVGL
K548 GLTAYVMKDPETRQL
K577 CCIDEFDKMNESTRS
K599 QQTLSIAKAGIICQL
K626 IESQWNPKKTTIENI
K626 IESQWNPKKTTIENI
K627 ESQWNPKKTTIENIQ
S706 PRLSEEASQALIEAY
Y713 SQALIEAYVNMRKIG
K745 RLAEAHAKVRFSDKV
K751 AKVRFSDKVEAIDVE
K761 AIDVEEAKRLHREAL
K761 AIDVEEAKRLHREAL
K769 RLHREALKQSATDPR
S783 RTGIVDISILTTGMS
T787 VDISILTTGMSATSR
R805 EELAEALRKLILSKG
K813 KLILSKGKTPALKYQ
K818 KGKTPALKYQQLFED
K818 KGKTPALKYQQLFED
K836 QSDTAITKDMFEEAL
K857 DFLTVTGKTVRLL__
K857 DFLTVTGKTVRLL__
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