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Protein Page:
ACLY (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
ACLY ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine. Homotetramer. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Carbohydrate Metabolism - citrate (TCA) cycle; Lyase; Transferase; EC 2.3.3.8
Cellular Component: mitochondrion; citrate lyase complex; cytoplasm; plasma membrane; nucleolus; cytosol; nucleus
Molecular Function: protein binding; ATP citrate synthase activity; metal ion binding; succinate-CoA ligase (ADP-forming) activity; cofactor binding; ATP binding
Biological Process: ATP catabolic process; coenzyme A metabolic process; lipid biosynthetic process; energy reserve metabolic process; positive regulation of cellular metabolic process; citrate metabolic process; triacylglycerol biosynthetic process; cellular carbohydrate metabolic process; cellular lipid metabolic process
Reference #:  P53396 (UniProtKB)
Alt. Names/Synonyms: ACL; ACLY; ATP citrate lyase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPCL; Citrate cleavage enzyme; CLATP
Gene Symbols: ACLY
Molecular weight: 120,839 Da
Basal Isoelectric point: 6.95  Predict pI for various phosphorylation states
CST Pathways:  Insulin Receptor Signaling  |  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

ACLY

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 2 K12-u AISEQTGkELLYKFI
0 2 K58-u LSQNLVVkPDQLIkR
0 1 K64-u VkPDQLIkRRGkLGL
0 2 K68-u QLIkRRGkLGLVGVN
0 1 T77 GLVGVNLTLDGVKSW
0 1 K86-a DGVKSWLkPRLGQEA
0 3 K86-u DGVKSWLkPRLGQEA
0 1 K97-u GQEATVGkATGFLKN
0 11 Y131-p YATREGDyVLFHHEG
0 3 K148-u DVGDVDAkAQkLLVG
0 3 K151-u DVDAkAQkLLVGVDE
0 1 K159 LLVGVDEKLNPEDIK
0 2 K230-u ATADYICkVkWGDIE
0 1 K232-u ADYICkVkWGDIEFP
0 2 Y247-p PPFGREAyPEEAYIA
0 2 K259-u YIADLDAksGASLkL
0 1 S260-p IADLDAksGASLkLT
0 1 S263 LDAksGASLkLTLLN
0 2 K265-u AksGASLkLTLLNPk
0 3 K272-u kLTLLNPkGRIWTMV
0 1 K369-u RDYQGPLkEHEVTIF
1 4 Y384-p VRRGGPNyQEGLRVM
0 1 S442-p ANFLLNAsGStstPA
0 2 T445-p LLNAsGStstPAPsR
0 7 S446-p LNAsGStstPAPsRt
1 53 T447-p NAsGStstPAPsRtA
1 8 S451-p StstPAPsRtAsFsE
0 12 T453-p stPAPsRtAsFsEsR
4 143 S455-p PAPsRtAsFsEsRAD
0 6 S457-p PsRtAsFsEsRADEV
0 4 S459-p RtAsFsEsRADEVAP
0 3 K471-u VAPAKKAkPAMPQDs
0 8 S478-p kPAMPQDsVPsPRSL
0 45 S481-p MPQDsVPsPRSLQGk
0 7 K488-u sPRSLQGkSTTLFSR
0 1 K498-u TLFSRHTkAIVWGMQ
0 1 T506 AIVWGMQTRAVQGML
0 3 Y531-p PSVAAMVyPFTGDHk
0 1 T534 AAMVyPFTGDHkQkF
0 2 K538-u yPFTGDHkQkFYWGH
1 0 K540-a FTGDHkQkFYWGHkE
1 1 K546-a QkFYWGHkEILIPVF
0 3 K546-u QkFYWGHkEILIPVF
1 3 K554-a EILIPVFkNMADAMR
0 4 Y588-p STMETMNyAQIRTIA
0 1 K616 LIKKADQKGVTIIGP
0 1 K630 PATVGGIKPGCFkIG
0 2 K635-a GIKPGCFkIGNtGGM
0 1 T639-p GCFkIGNtGGMLDNI
0 3 K650-u LDNILASkLyRPGSV
0 2 Y652-p NILASkLyRPGSVAY
0 3 S663-p SVAYVSRsGGMSNEL
0 362 Y682-p SRTTDGVyEGVAIGG
3 0 H760-p SSEVQFGhAGACANQ
0 5 K780-u VAKNQALkEAGVFVP
0 2 Y825 PPTVPMDYSWARELG
0 1 K836 RELGLIRKPAsFMTS
0 2 S839-p GLIRKPAsFMTSICD
0 8 K918-u IICARAGkDLVSSLT
0 3 K944-u GALDAAAkMFSkAFD
0 1 K948-a AAAkMFSkAFDSGII
0 2 K948-u AAAkMFSkAFDSGII
0 1 S952 MFSkAFDSGIIPMEF
0 1 K962-a IPMEFVNkMKKEGkL
0 1 K962 IPMEFVNKMKKEGkL
0 2 K968-a NkMKKEGkLIMGIGH
0 2 K978-a MGIGHRVksINNPDM
0 1 K978 MGIGHRVKsINNPDM
0 2 S979-p GIGHRVksINNPDMR
0 4 K991-u DMRVQILkDYVRQHF
0 63 Y1073-p SMGFIGHyLDQkRLk
0 7 K1077-a IGHyLDQkRLkQGLY
0 3 K1077-u IGHyLDQkRLkQGLY
0 3 K1080-u yLDQkRLkQGLYRHP
0 5 S1100-p YVLPEHMsM______
4331 : Phospho-ATP-Citrate Lyase (Ser455) Antibody
  ACLY iso3  
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
S52 ANFLLNASGSTSTPA
T55 LLNASGSTSTPAPSR
S56 LNASGSTSTPAPSRT
T57 NASGSTSTPAPSRTA
S61 STSTPAPSRTASFSE
T63 STPAPSRTASFSESR
S65 PAPSRTASFSESRAD
S67 PSRTASFSESRADEV
S69 RTASFSESRADEVAP
K81-u VAPAKKAkPAMPQGk
- gap
- gap
K88-u kPAMPQGkSTTLFSR
K98 TLFSRHTKAIVWGMQ
T106 AIVWGMQTRAVQGML
Y131 PSVAAMVYPFTGDHK
T134 AAMVYPFTGDHKQKF
K138 YPFTGDHKQKFYWGH
K140 FTGDHKQKFYWGHKE
K146 QKFYWGHKEILIPVF
K146 QKFYWGHKEILIPVF
K154 EILIPVFKNMADAMR
Y188 STMETMNYAQIRTIA
K216 LIKKADQKGVTIIGP
K230 PATVGGIKPGCFKIG
K235 GIKPGCFKIGNTGGM
T239 GCFKIGNTGGMLDNI
K250 LDNILASKLYRPGSV
Y252 NILASKLYRPGSVAY
S263 SVAYVSRSGGMSNEL
Y282 SRTTDGVYEGVAIGG
H360 SSEVQFGHAGACANQ
K380 VAKNQALKEAGVFVP
Y425 PPTVPMDYSWARELG
K436 RELGLIRKPASFMTS
S439 GLIRKPASFMTSICD
K518 IICARAGKDLVSSLT
K544 GALDAAAKMFSKAFD
K548 AAAKMFSKAFDSGII
K548 AAAKMFSKAFDSGII
S552 MFSKAFDSGIIPMEF
K562 IPMEFVNKMKKEGKL
K562 IPMEFVNKMKKEGKL
K568 NKMKKEGKLIMGIGH
K578 MGIGHRVKSINNPDM
K578 MGIGHRVKSINNPDM
S579 GIGHRVKSINNPDMR
K591 DMRVQILKDYVRQHF
Y673 SMGFIGHYLDQKRLK
K677 IGHYLDQKRLKQGLY
K677 IGHYLDQKRLKQGLY
K680 YLDQKRLKQGLYRHP
S700 YVLPEHMSM______
  mouse

► Hide Isoforms
 
K12-u AISEQTGkELLYKYI
K58 LSQSLVVKPDQLIKR
K64 VKPDQLIKRRGkLGL
K68-u QLIKRRGkLGLVGVN
S77-p GLVGVNLsLDGVKSW
K86 DGVKSWLKPRLGHEA
K86 DGVKSWLKPRLGHEA
K97 GHEATVGKAKGFLKN
Y131 YATREGDYVLFHHEG
K148-u DVGDVDAkAQkLLVG
K151-u DVDAkAQkLLVGVDE
K159-u LLVGVDEkLNTEDIK
K230-u ATADYICkVKWGDIE
K232 ADYICkVKWGDIEFP
Y247-p PPFGREAyPEEAYIA
K259-u YIADLDAksGAsLkL
S260-p IADLDAksGAsLkLT
S263-p LDAksGAsLkLTLLN
K265-u AksGAsLkLTLLNPk
K272-u kLTLLNPkGRIWTMV
K369 RDYQGPLKEHEVTIF
Y384-p VRRGGPNyQEGLRVM
S442 ANFLLNASGSTStPA
T445 LLNASGSTStPAPSR
S446 LNASGSTStPAPSRt
T447-p NASGSTStPAPSRtA
S451 STStPAPSRtAsFsE
T453-p StPAPSRtAsFsEsR
S455-p PAPSRtAsFsEsRAD
S457-p PSRtAsFsEsRADEV
S459-p RtAsFsEsRADEVAP
K471-u VAPAKKAkPAMPQGk
- gap
- gap
K478-u kPAMPQGkSATLFSR
K488 TLFSRHTKAIVWGMQ
T496-p AIVWGMQtRAVQGML
Y521-p PSVAAMVyPFtGDHk
T524-p AAMVyPFtGDHkQKF
K528-u yPFtGDHkQKFYWGH
K530 FtGDHkQKFYWGHkE
K536 QKFYWGHKEILIPVF
K536-u QKFYWGHkEILIPVF
K544-a EILIPVFkNMADAMK
Y578 STMETMNYAQIRTIA
K606-u LIKKADQkGVTIIGP
K620-u PATVGGIkPGCFKIG
K625 GIkPGCFKIGNTGGM
T629 GCFKIGNTGGMLDNI
K640-u LDNILASkLYRPGSV
Y642 NILASkLYRPGSVAY
S653-p SVAYVSRsGGMSNEL
Y672-p SRTTDGVyEGVAIGG
H750-p SSEVQFGhAGACANQ
K770-u VAKNQALkEAGVFVP
Y815-p PPTVPMDySWARELG
K826-u RELGLIRkPAsFMTS
S829-p GLIRkPAsFMTSICD
K908-u IICARAGkDLVSSLT
K934-u GALDAAAkMFSkAFD
K938 AAAkMFSKAFDsGII
K938-u AAAkMFSkAFDsGII
S942-p MFSkAFDsGIIPMEF
K952 IPMEFVNKMKKEGkL
K952-u IPMEFVNkMKKEGkL
K958-a NkMKKEGkLIMGIGH
K968-a MGIGHRVksINNPDM
K968-u MGIGHRVksINNPDM
S969-p GIGHRVksINNPDMR
K981-u DMRVQILkDFVKQHF
Y1063 SMGFIGHYLDQkRLk
K1067-a IGHYLDQkRLkQGLY
K1067-u IGHYLDQkRLkQGLY
K1070-u YLDQkRLkQGLYRHP
S1090-p YVLPEHMsM______
4331 : Phospho-ATP-Citrate Lyase (Ser455) Antibody
  ACLY iso2  
K12 AISEQTGKELLYKYI
K58 LSQSLVVKPDQLIKR
K64 VKPDQLIKRRGKLGL
K68 QLIKRRGKLGLVGVN
S77 GLVGVNLSLDGVKSW
K86 DGVKSWLKPRLGHEA
K86 DGVKSWLKPRLGHEA
K97 GHEATVGKAKGFLKN
Y131 YATREGDYVLFHHEG
K148 DVGDVDAKAQKLLVG
K151 DVDAKAQKLLVGVDE
K159 LLVGVDEKLNTEDIK
K230 ATADYICKVKWGDIE
K232 ADYICKVKWGDIEFP
Y247 PPFGREAYPEEAYIA
K259 YIADLDAKSGASLKL
S260 IADLDAKSGASLKLT
S263 LDAKSGASLKLTLLN
K265 AKSGASLKLTLLNPK
K272 KLTLLNPKGRIWTMV
K369 RDYQGPLKEHEVTIF
Y384 VRRGGPNYQEGLRVM
S442 ANFLLNASGSTSTPA
T445 LLNASGSTSTPAPSR
S446 LNASGSTSTPAPSRT
T447 NASGSTSTPAPSRTA
S451 STSTPAPSRTASFSE
T453 STPAPSRTASFSESR
S455 PAPSRTASFSESRAD
S457 PSRTASFSESRADEV
S459 RTASFSESRADEVAP
K471 VAPAKKAKPAMPQDS
S478 KPAMPQDSVPsPRSL
S481-p MPQDSVPsPRSLQGk
K488-u sPRSLQGkSATLFSR
K498 TLFSRHTKAIVWGMQ
T506 AIVWGMQTRAVQGML
Y531 PSVAAMVYPFTGDHK
T534 AAMVYPFTGDHKQKF
K538 YPFTGDHKQKFYWGH
K540 FTGDHKQKFYWGHKE
K546 QKFYWGHKEILIPVF
K546 QKFYWGHKEILIPVF
K554 EILIPVFKNMADAMK
Y588 STMETMNYAQIRTIA
K616 LIKKADQKGVTIIGP
K630 PATVGGIKPGCFKIG
K635 GIKPGCFKIGNTGGM
T639 GCFKIGNTGGMLDNI
K650 LDNILASKLYRPGSV
Y652 NILASKLYRPGSVAY
S663 SVAYVSRSGGMSNEL
Y682 SRTTDGVYEGVAIGG
H760 SSEVQFGHAGACANQ
K780 VAKNQALKEAGVFVP
Y825 PPTVPMDYSWARELG
K836 RELGLIRKPASFMTS
S839 GLIRKPASFMTSICD
K918 IICARAGKDLVSSLT
K944 GALDAAAKMFSKAFD
K948 AAAKMFSKAFDSGII
K948 AAAKMFSKAFDSGII
S952 MFSKAFDSGIIPMEF
K962 IPMEFVNKMKKEGKL
K962 IPMEFVNKMKKEGKL
K968 NKMKKEGKLIMGIGH
K978 MGIGHRVKSINNPDM
K978 MGIGHRVKSINNPDM
S979 GIGHRVKSINNPDMR
K991 DMRVQILKDFVKQHF
Y1073 SMGFIGHYLDQKRLK
K1077 IGHYLDQKRLKQGLY
K1077 IGHYLDQKRLKQGLY
K1080 YLDQKRLKQGLYRHP
S1100 YVLPEHMSM______
  ACLY iso3  
K12 AISEQTGKELLYKYI
K58 LSQSLVVKPDQLIKR
K64 VKPDQLIKRRGKLGL
K68 QLIKRRGKLGLVGVN
S77 GLVGVNLSLDGVKSW
K86 DGVKSWLKPRLGHEA
K86 DGVKSWLKPRLGHEA
K97 GHEATVGKAKGFLKN
Y131 YATREGDYVLFHHEG
K148 DVGDVDAKAQKLLVG
K151 DVDAKAQKLLVGVDE
K159 LLVGVDEKLNTEDIK
K230 ATADYICKVKWGDIE
K232 ADYICKVKWGDIEFP
Y247 PPFGREAYPEEAYIA
K259 YIADLDAKSGASLKL
S260 IADLDAKSGASLKLT
S263 LDAKSGASLKLTLLN
K265 AKSGASLKLTLLNPK
K272 KLTLLNPKGRIWTMV
K369 RDYQGPLKEHEVTIF
Y384 VRRGGPNYQEGLRVM
S442 ANFLLNASGSTSTPA
T445 LLNASGSTSTPAPSR
S446 LNASGSTSTPAPSRT
T447 NASGSTSTPAPSRTA
S451 STSTPAPSRTASFSE
T453 STPAPSRTASFSESR
S455 PAPSRTASFSESRAD
S457 PSRTASFSESRADEV
S459 RTASFSESRADEVAP
K471 VAPAKKAKPAMPQDS
S478 KPAMPQDSVPsPRSL
S481-p MPQDSVPsPRSLQGH
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  rat

 
K12 AISEQTGKELLYKYI
K58 LSQSLVVKPDQLIKR
K64 VKPDQLIKRRGKLGL
K68 QLIKRRGKLGLVGVN
S77 GLVGVNLSLDGVKSW
K86 DGVKSWLKPRLGHEA
K86-u DGVKSWLkPRLGHEA
K97 GHEATVGKAKGFLKN
Y131 YATREGDYVLFHHEG
K148 DVGDVDTKAQKLLVG
K151 DVDTKAQKLLVGVDE
K159 LLVGVDEKLNAEDIK
K230 ATADYICKVKWGDIE
K232 ADYICKVKWGDIEFP
Y247 PPFGREAYPEEAYIA
K259 YIADLDAKSGASLKL
S260 IADLDAKSGASLKLT
S263 LDAKSGASLKLTLLN
K265 AKSGASLKLTLLNPK
K272 KLTLLNPKGRIWTMV
K369 RDYQGSLKEHEVTIF
Y384-p VRRGGPNyQEGLRVM
S441 ANFLLNASGSTStPA
T444 LLNASGSTStPAPsR
S445 LNASGSTStPAPsRt
T446-p NASGSTStPAPsRtA
S450-p STStPAPsRtAsFSE
T452-p StPAPsRtAsFSEsR
S454-p PAPsRtAsFSEsRAD
S456 PsRtAsFSEsRADEV
S458-p RtAsFSEsRADEVAP
K470 VAPAKKAKPAMPQDs
S477-p KPAMPQDsVPsPRSL
S480-p MPQDsVPsPRSLQGK
K487 sPRSLQGKSATLFSR
K497 TLFSRHTKAIVWGMQ
T505 AIVWGMQTRAVQGML
Y530 PSVAAMVYPFTGDHK
T533 AAMVYPFTGDHKQKF
K537 YPFTGDHKQKFYWGH
K539 FTGDHKQKFYWGHKE
K545 QKFYWGHKEILIPVF
K545 QKFYWGHKEILIPVF
K553-a EILIPVFkNMADAMK
Y587 STMETMNYAQIRTIA
K615 LIKKADQKGVTIIGP
K629 PATVGGIKPGCFKIG
K634 GIKPGCFKIGNTGGM
T638 GCFKIGNTGGMLDNI
K649 LDNILASKLYRPGSV
Y651 NILASKLYRPGSVAY
S662 SVAYVSRSGGMSNEL
Y681-p SRTTDGVyEGVAIGG
H759-p SSEVQFGhAGACANQ
K779 VAKNQALKEAGVFVP
Y824 PPTVPMDYSWARELG
K835 RELGLIRKPASFMTS
S838 GLIRKPASFMTSICD
K917 IICARAGKDLVSSLT
K943 GALDAAAKMFSKAFD
K947 AAAKMFSKAFDSGII
K947 AAAKMFSKAFDSGII
S951 MFSKAFDSGIIPMEF
K961 IPMEFVNKMKKEGKL
K961 IPMEFVNKMKKEGKL
K967 NKMKKEGKLIMGIGH
K977 MGIGHRVKSINNPDM
K977 MGIGHRVKSINNPDM
S978 GIGHRVKSINNPDMR
K990 DMRVQILKDFVKQHF
Y1072 SMGFIGHYLDQKRLK
K1076 IGHYLDQKRLKQGLY
K1076 IGHYLDQKRLKQGLY
K1079 YLDQKRLKQGLYRHP
S1099 YVLPEHMSM______
4331 : Phospho-ATP-Citrate Lyase (Ser455) Antibody
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