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Protein Page:
KPNB1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
KPNB1 Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re- exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports PRKCI into the nucleus. Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones. Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Binds to HIV-1 Rev and Tat. Interacts with HTLV-1 Rex. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota. Interacts with human respiratory syncytial virus (HRSV) protein M. Interacts with KPNA7. Belongs to the importin beta family. Note: This description may include information from UniProtKB.
Protein type: Karyopherin; Nuclear import
Cellular Component: nucleoplasm; nuclear membrane; cytoplasm; nucleolus; nuclear envelope; nuclear pore; cytosol; nucleus
Molecular Function: protein domain specific binding; protein binding; enzyme binding; zinc ion binding; nuclear localization sequence binding; Ran GTPase binding; protein transporter activity
Biological Process: ribosomal protein import into nucleus; viral reproduction; apoptosis; protein import into nucleus; protein import into nucleus, translocation; cytokine and chemokine mediated signaling pathway; NLS-bearing substrate import into nucleus; viral infectious cycle; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis
Reference #:  Q14974 (UniProtKB)
Alt. Names/Synonyms: IMB1; Impnb; importin 1; importin 90; importin beta-1 subunit; Importin subunit beta-1; Importin-90; IPO1; IPOB; karyopherin (importin) beta 1; Karyopherin subunit beta-1; KPNB1; MGC2155; MGC2156; MGC2157; NTF97; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97
Gene Symbols: KPNB1
Molecular weight: 97,170 Da
Basal Isoelectric point: 4.68  Predict pI for various phosphorylation states
CST Pathways:  ErbB/HER Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

KPNB1

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
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Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 T5-p ___MELItILEKtVs
0 1 T10-p LItILEKtVsPDRLE
0 5 S12-p tILEKtVsPDRLELE
0 3 K23-u LELEAAQkFLERAAV
0 6 K62-u VAAGLQIkNSLTSkD
0 2 K68-u IkNSLTSkDPDIKAQ
1 1 Y76-p DPDIKAQyQQRWLAI
1 3 K206-u LNSLEFTkANFDkES
1 1 K211-u FTkANFDkESERHFI
0 1 K211-a FTkANFDkESERHFI
0 4 K376-u PFIKEHIkNPDWRYR
0 1 Y529-p NNLRSSAyESLMEIV
0 50 K537-u ESLMEIVkNSAkDCY
0 25 K541-u EIVkNSAkDCYPAVQ
0 9 K549-u DCYPAVQkTTLVIME
0 4 K651-u LKYMEAFkPFLGIGL
0 12 K710-u ENVHRSVkPQILSVF
0 3 K730-u AIGGEFKkYLEVVLN
0 4 S750-p SQAQVDKsDyDMVDY
0 25 Y752-p AQVDKsDyDMVDYLN
0 12 K777-u TGIVQGLkGDQENVH
0 1 K835-a AFGKDVLkLVEARPM
0 8 K835-u AFGKDVLkLVEARPM
0 1 S853-p LLTEGRRsKtNKAKT
0 1 T855-p TEGRRsKtNKAKTLA
0 9 K867-a TLATWATkELRKLKN
0 1 K867-u TLATWATkELRKLKN
  mouse

 
T5 ___MELITILEKTVS
T10 LITILEKTVSPDRLE
S12 TILEKTVSPDRLELE
K23-u LELEAAQkFLERAAV
K62-u VAAGLQIkNSLTSkD
K68-u IkNSLTSkDPDIKAQ
Y76 DPDIKAQYQQRWLAI
K206 LNSLEFTKANFDKES
K211 FTKANFDKESERHFI
K211 FTKANFDKESERHFI
K376-u PFIKEHIkNPDWRYR
Y529 NNLRSSAYESLMEIV
K537-u ESLMEIVkNSAKDCY
K541 EIVkNSAKDCYPAVQ
K549 DCYPAVQKTTLVIME
K651 LKYMEAFKPFLGIGL
K710-u ENVHRSVkPQILSVF
K730 AIGGEFKKYLEVVLN
S750 SQAQVDKSDFDMVDY
F752 AQVDKSDFDMVDYLN
K777 TGIVQGLKGDQENVH
K835 AFGKDVLKLVEARPM
K835-u AFGKDVLkLVEARPM
S853 LLTEGRRSKTNKAKT
T855 TEGRRSKTNKAKTLA
K867 TLATWATKELRKLKN
K867 TLATWATKELRKLKN
  rat

 
T5 ___MELITILEKTVS
T10 LITILEKTVSPDRLE
S12 TILEKTVSPDRLELE
K23 LELEAAQKFLERAAV
R62 VAAGLQIRLLTSKDP
K67 QIRLLTSKDPDIKAQ
Y75 DPDIKAQYQQRWLAI
K205 LNSLEFTKANFDKES
K210 FTKANFDKESERHFI
K210 FTKANFDKESERHFI
K375 PFIKEHIKNPDWRYR
Y528 NNLRSSAYESLMEIV
K536 ESLMEIVKNSAKDCY
K540 EIVKNSAKDCYPAVQ
K548 DCYPAVQKTTLVIME
K650 LKYMEAFKPFLGIGL
K709 ENVHRSVKPQILSVF
K729 AIGGEFKKYLEVVLN
S749 SQAQVDKSDFDMVDY
F751 AQVDKSDFDMVDYLN
K776 TGIVQGLKGDQENVH
K834 AFGKDVLKLVEARPM
K834 AFGKDVLKLVEARPM
S852 LLTEGRRSKTNKAKT
T854 TEGRRSKTNKAKTLA
K866 TLATWATKELRKLKN
K866 TLATWATKELRKLKN
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