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Protein Page:
UBE2D3 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
UBE2D3 Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA- linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML- NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction. Interacts with SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex; when Cullin is neddylated, the interaction between the E2 and the SCF complex is strengthened. Interacts with DAPK3. Interacts with BRCA1; the DNA damage checkpoint promotes the association with BRCA1 after ionizing radiation. Interacts non- covalently with ubiquitin. Belongs to the ubiquitin-conjugating enzyme family. 3 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ligase; Ubiquitin ligase
Cellular Component: nucleoplasm; plasma membrane; endosome membrane; cytosol
Molecular Function: protein binding; acid-amino acid ligase activity; ubiquitin-protein ligase activity; ATP binding
Biological Process: protein monoubiquitination; ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; transcription initiation from RNA polymerase II promoter; protein polyubiquitination; transcription, DNA-dependent; apoptosis; MyD88-independent toll-like receptor signaling pathway; protein modification process; protein ubiquitination; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; DNA repair; BMP signaling pathway; transforming growth factor beta receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; gene expression; toll-like receptor 4 signaling pathway; negative regulation of interferon type I production
Reference #:  P61077 (UniProtKB)
Alt. Names/Synonyms: E2(17)KB 3; E2(17)KB3; MGC43926; MGC5416; UB2D3; UBC4/5; UBCH5C; UBE2D3; Ubiquitin carrier protein D3; Ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); Ubiquitin-protein ligase D3
Gene Symbols: UBE2D3
Molecular weight: 16,687 Da
Basal Isoelectric point: 7.67  Predict pI for various phosphorylation states
CST Pathways:  NF-kB Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
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UBE2D3

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 30 K8-ub MALKRINkELSDLAR
0 3 S11 KRINkELSDLARDPP
0 2 S83 PNINSNGSICLDILR
0 2 T98-p SQWSPALtISkVLLS
0 1 K101-ub SPALtISkVLLSICS
0 50 K128-ub PEIARIYkTDRDkYN
0 1 K128-sc PEIARIYkTDRDkYN
0 2 K133-ac IYkTDRDkYNRISRE
0 2 K133-ub IYkTDRDkYNRISRE
0 58 K144-ub ISREWTQkYAM____
  UBE2D3 iso3  
K10 SNRKCLSKELsDLAR
S13-p KCLSKELsDLARDPP
S85 PNINSNGSICLDILR
T100 SQWSPALTISKVLLS
K103 SPALTISKVLLSICS
K130 PEIARIYKTDRDKYN
K130 PEIARIYKTDRDKYN
K135 IYKTDRDKYNRISRE
K135 IYKTDRDKYNRISRE
K146 ISREWTQKYAM____
  mouse

 
K8-ub MALKRINkELsDLAR
S11-p KRINkELsDLARDPP
S83-p PNINSNGsICLDILR
T98 SQWSPALTISKVLLS
K101 SPALTISKVLLSICS
K128-ub PEIARIYkTDRDkYN
K128 PEIARIYKTDRDkYN
K133-ac IYkTDRDkYNRISRE
K133-ub IYkTDRDkYNRISRE
K144-ub ISREWTQkYAM____
  rat

 
K8 MALKRINKELSDLAR
S11 KRINKELSDLARDPP
S83 PNINSNGSICLDILR
T98-p SQWSPALtISKVLLS
K101 SPALtISKVLLSICS
K128-ub PEIARIYkTDRDKYN
K128 PEIARIYKTDRDKYN
K133 IYkTDRDKYNRISRE
K133 IYkTDRDKYNRISRE
K144 ISREWTQKYAM____
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