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Protein Page:
APAF (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
APAF Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. Monomer. Oligomerizes upon binding of cytochrome c and dATP. Oligomeric Apaf-1 and pro-caspase-9 bind to each other via their respective NH2-terminal CARD domains and consecutively mature caspase-9 is released from the complex. Pro-caspase-3 is recruited into the Apaf-1-pro-caspase-9 complex via interaction with pro-caspase-9. Interacts with APIP. Interacts (via CARD and NACHT domains) with NAIP/BIRC1 (via NACHT domain). By E2F and p53/TP53 in apoptotic neurons. Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver. 6 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Apoptosis
Chromosomal Location of Human Ortholog: 12q23
Cellular Component: cytosol; nucleus; apoptosome
Molecular Function: identical protein binding; protein binding; nucleotide binding; caspase activator activity; ADP binding; ATP binding
Biological Process: regulation of apoptosis; caspase activation; nervous system development; neuron apoptosis; positive regulation of apoptosis; apoptosis; forebrain development; neural tube closure; defense response; DNA fragmentation during apoptosis; caspase activation via cytochrome c
Reference #:  O14727 (UniProtKB)
Alt. Names/Synonyms: APAF; APAF-1; APAF1; apoptotic peptidase activating factor 1; apoptotic protease activating factor 1; Apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413
Gene Symbols: APAF1
Molecular weight: 141,840 Da
Basal Isoelectric point: 5.96  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  Mitochondrial Control of Apoptosis
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

APAF

Protein Structure Not Found.


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Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

► Hide Isoforms
 
0 1 Y24-p EKDIKTSyIMDHMIs
0 1 S31-p yIMDHMIsDGFLTIS
0 1 T48-p EKVRNEPtQQQRAAM
0 1 K224-ub PLNIEEAkDRLRILM
0 4 S238-p MLRKHPRsLLILDDV
0 4 S248-p ILDDVWDsWVLKAFD
1 0 S268-p LLTTRDKsVTDsVMG
0 1 S272-p RDKsVTDsVMGPKyV
0 1 Y278-p DsVMGPKyVVPVESS
0 1 K288-ub PVESSLGkEKGLEIL
0 7 S320-p IIKECKGsPLVVSLI
1 0 S357-p FKRIRKSsSYDYEAL
0 1 S369-p EALDEAMsIsVEMLR
0 1 S371-p LDEAMsIsVEMLRED
0 1 Y382-p LREDIKDyyTDLSIL
0 1 Y383-p REDIKDyyTDLSILQ
0 1 K643-ac DKTLQVFkAETGEKL
0 3 K654-ac GEKLLEIkAHEDEVL
0 1 N789 NVKQFFLNLEDPQED
0 1 K820 IMVAAKNKIFLFDIH
0 1 S901-p GSSFLTSsDDQtIRL
0 1 T905-p LTSsDDQtIRLWETK
0 1 T1103-p DATKFSStSADKtAK
0 1 T1108-p SStSADKtAKIWSFD
  APAF iso4  
Y24 EKDIKTSYIMDHMIS
S31 YIMDHMISDGFLTIS
T48 EKVRNEPTQQQRAAM
K224 PLNIEEAKDRLRILM
S238 MLRKHPRSLLILDDV
S248 ILDDVWDSWVLKAFD
S268 LLTTRDKSVTDSVMG
S272 RDKSVTDSVMGPKYV
Y278 DSVMGPKYVVPVESS
K288 PVESSLGKEKGLEIL
S320 IIKECKGSPLVVSLI
S357 FKRIRKSSSYDYEAL
S369 EALDEAMSISVEMLR
S371 LDEAMSISVEMLRED
Y382 LREDIKDYYTDLSIL
Y383 REDIKDYYTDLSILQ
K643 DKTLQVFKAETGEKL
K654 GEKLLEIKAHEDEVL
N789 NVKQFFLNLEDPQED
K820-ub IMVAAKNkIFLWNTD
S858 GSSFLTSSDDQTIRL
T862 LTSSDDQTIRLWETK
T1060 DATKFSSTSADKTAK
T1065 SSTSADKTAKIWSFD
  APAF iso6  
Y24 EKDIKTSYIMDHMIS
S31 YIMDHMISDGFLTIS
T48 EKVRNEPTQQQRAAM
K224 PLNIEEAKDRLRILM
S238 MLRKHPRSLLILDDV
S248 ILDDVWDSWVLKAFD
S268 LLTTRDKSVTDSVMG
S272 RDKSVTDSVMGPKYV
Y278 DSVMGPKYVVPVESS
K288 PVESSLGKEKGLEIL
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
- gap
  mouse

 
Y24 EKDIKTSYIMDHMIS
S31 YIMDHMISNGVLSVI
T48 EKVKSQATQYQRAAA
K224 PLNIEEAKDRLRVLM
S238 MLRKHPRSLLILDDV
P248 ILDDVWDPWVLKAFD
S268 LLTTRDKSVTDSVMG
S272 RDKSVTDSVMGPKHV
H278 DSVMGPKHVVPVESG
R288 PVESGLGREKGLEIL
S320 IIKECKGSPLVVSLI
S357 FKRIRKSSSYDYEAL
S369 EALDEAMSISVEMLR
S371 LDEAMSISVEMLRED
Y382 LREDIKDYYTDLSIL
Y383 REDIKDYYTDLSILQ
K643 DKTLQVFKAETGEKL
K654 GEKLLDIKAHEDEVL
S789-p NVKRFFLsSEDPPED
K820 IIVAAKNKVLLFDIH
S901 GSSFLTASDDQTIRV
T905 LTASDDQTIRVWETK
T1103 DATKFSSTSADKTAK
T1108 SSTSADKTAKIWSFD
  rat

 
Y24 EKDIKTSYIMDHMIS
S31 YIMDHMISNGVLTVV
T48 EKVKSQATQYQRAAA
K224 PLNIEEAKDRLRVLM
S238 MLRKHPRSLLILDDV
P248 ILDDVWDPWVLKAFD
S268 LLTTRDKSVTDSVMG
S272 RDKSVTDSVMGPKYV
Y278 DSVMGPKYVIPVESG
K288 PVESGLGKEKGLEIL
S320 IIKECKGSPLVVSLV
S357 FKRIRKSSSYDYEAL
S369 EALDEAMSISVEMLR
S371 LDEAMSISVEMLRED
Y382 LREDIKDYYTDLSIL
Y383 REDIKDYYTDLSILQ
K643 DKTLQVFKAETGEKL
K654 GEKLLDIKAHEDEVL
S789 NVKRFFLSSEDPPED
K820 IIVAAKNKVLLLDIH
S901 GSSFLTASDDQTIRV
T905 LTASDDQTIRVWETR
T1103 DATKFSSTSADKTAK
T1108 SSTSADKTAKIWSFE
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