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Protein Page:
NBS1 (human)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NBS1 a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; Cell cycle regulation
Chromosomal Location of Human Ortholog: 8q21
Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body
Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding
Biological Process: meiosis; DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; positive regulation of kinase activity; isotype switching; DNA repair; positive regulation of protein amino acid autophosphorylation; DNA duplex unwinding; double-strand break repair via homologous recombination; regulation of DNA replication initiation; cell proliferation; mitotic cell cycle G2/M transition DNA damage checkpoint; double-strand break repair; DNA damage checkpoint; blastocyst growth; neuromuscular process controlling balance; cell cycle arrest; telomere maintenance
Reference #:  O60934 (UniProtKB)
Alt. Names/Synonyms: AT-V1; AT-V2; ATV; Cell cycle regulatory protein p95; FLJ10155; MGC87362; NBN; NBS; NBS1; Nibrin; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; P95; p95 protein of the MRE11/RAD50 complex
Gene Symbols: NBN
Molecular weight: 84,959 Da
Basal Isoelectric point: 6.5  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint  |  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NBS1

Protein Structure Not Found.


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Sites Implicated In
apoptosis, induced: S278‑p, S343‑p
cell cycle regulation: S278‑p, S343‑p
cell growth, altered: S343‑p
chromatin organization, altered: S278‑p, S343‑p
acetylation: S343‑p
activity, induced: S343‑p
phosphorylation: S278‑p, S343‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 K3-ac _____MWkLLPAAGP
0 1 Y16-p GPAGGEPyRLLTGVE
0 11 S58-p NFSVTNLsQtDEIPV
0 2 T60-p SVTNLsQtDEIPVLT
0 1 S195-p KQPPQIEsFyPPLDE
0 1 Y197-p PPQIEsFyPPLDEPS
1 0 K208-ac DEPSIGSkNVDLSGR
1 0 K233-ac TFIFLNAkQHKKLSS
9 0 S278-p VDTGITNsQTLIPDC
0 1 S292-p CQKKWIQsIMDMLQR
1 0 K334-ac GHPSTGLktTtPGPs
0 1 T335-p HPSTGLktTtPGPsL
0 7 T337-p STGLktTtPGPsLsQ
0 5 S341-p ktTtPGPsLsQGVsV
41 66 S343-p TtPGPsLsQGVsVDE
0 5 S347-p PsLsQGVsVDEKLMP
0 1 K388-m1 PKEIKVSkMEQKFRM
1 73 S397-p EQKFRMLsQDAPtVK
0 2 A400 FRMLsQDAPtVKESC
0 1 T402-p MLsQDAPtVKESCKT
0 1 S418-p SNNNSMVsNtLAKMR
0 1 T420-p NNSMVsNtLAKMRIP
0 1 I426 NtLAKMRIPNyQLsP
0 1 N428 LAKMRIPNyQLsPtK
0 2 Y429-p AKMRIPNyQLsPtKL
1 19 S432-p RIPNyQLsPtKLPsI
0 3 T434-p PNyQLsPtKLPsINk
0 1 S438-p LsPtKLPsINksKDR
1 0 K441-ac tKLPsINksKDRAsQ
0 3 S442-p KLPsINksKDRAsQQ
0 1 S447-p NksKDRAsQQQQTNS
0 1 S462-p IRNYFQPsTKKRERD
0 1 S488-p ARIETSCsLLEQTQP
0 8 T497-p LEQTQPAtPSLWKNk
1 0 K504-ac tPSLWKNkEQHLsEN
0 5 S509-p KNkEQHLsENEPVDt
0 1 T516-p sENEPVDtNsDNNLF
0 3 S518-p NEPVDtNsDNNLFTD
0 3 N534 DLKSIVKNSASKSHA
1 4 K544-ac SKSHAAEkLRSNKkR
0 1 K550-ac EkLRSNKkREMDDVA
0 1 K568-ac EVLEQLFkDTKPELE
0 2 T602-p MDIETNDtFsDEAVP
0 4 S604-p IETNDtFsDEAVPEs
0 1 A607 NDtFsDEAVPEssKI
0 1 S611-p sDEAVPEssKIsQEN
0 4 S612-p DEAVPEssKIsQENE
1 13 S615-p VPEssKIsQENEIGK
1 0 K665-ac EFRSLVIkNSTSRNP
0 1 K665-ub EFRSLVIkNSTSRNP
0 1 S673-p NSTSRNPsGINDDYG
0 2 K683-ub NDDYGQLkNFKKFKk
1 0 K690-ac kNFKKFKkVTyPGAG
0 1 Y693-p KKFKkVTyPGAGkLP
1 0 K698-ac VTyPGAGkLPHIIGG
0 1 S706-p LPHIIGGsDLIAHHA
1 0 K715-ac LIAHHARkNTELEEW
3001 : Phospho-p95/NBS1 (Ser343) Antibody
  mouse

 
K3 _____MWKLLPAAGA
Y16 GAAPGEPYRLLAGVE
S58 NFPVTSLSQTDEIPT
T60 PVTSLSQTDEIPTLT
S195 KQPPDIESFYPPIDE
Y197 PPDIESFYPPIDEPA
K208 DEPAIGSKSVDLSGR
K233 TFVFLNAKQHKKLSS
T278-p VDVGITNtQLIISHS
L292 SQKKWIHLIMDTLQR
K334 GQPCTELKTTTPGPS
T335 QPCTELKTTTPGPSL
T337 CTELKTTTPGPSLSQ
S341 KTTTPGPSLSQVLSA
S343 TTPGPSLSQVLSANG
S347 PSLSQVLSANGKIIP
G389 PEEVKIPGLEQSSRK
S398-p EQSSRKLsQEtFNIK
T401-p SRKLsQEtFNIKEAP
N403 KLsQEtFNIKEAPKP
S419 SKANNVASDTLVRGK
T421 ANNVASDTLVRGKtP
T427-p DTLVRGKtPsYQLsP
S429-p LVRGKtPsYQLsPMK
Y430 VRGKtPsYQLsPMKF
S433-p KtPsYQLsPMKFPVA
M435 PsYQLsPMKFPVANK
V439 LsPMKFPVANKNKDW
K442 MKFPVANKNKDWTSQ
N443 KFPVANKNKDWTSQQ
S448 NKNKDWTSQQQQNSI
C462 IKNYFQPCTRKRERD
S488 SRMELSCSLLEQTQP
G497 LEQTQPAGPSLWKSK
K504 GPSLWKSKEHQSQNA
S508 WKSKEHQSQNATLDR
R515 SQNATLDREADTSSV
A517 NATLDREADTSSVGG
S533-p DIELNRKsPDRKPLP
T543 RKPLPTETLRPRKRK
R549 ETLRPRKRKDVDLAT
R566 EVLEELLRSTKPELA
P600 MDAERNRPLNGGsEP
N602 AERNRPLNGGsEPES
S605-p NRPLNGGsEPESNSA
S609 NGGsEPESNSALQED
N610 GGsEPESNSALQEDE
L613 EPESNSALQEDEREK
H665 RSLVVSNHNSTSRNL
H665 RSLVVSNHNSTSRNL
C673 NSTSRNLCVNECGPL
K681 VNECGPLKNFKKFKK
K688 KNFKKFKKATFPGAG
F691 KKFKKATFPGAGKLP
K696 ATFPGAGKLPHIIGG
S704 LPHIIGGSDLVGHHA
K713 LVGHHARKNTELEEW
  rat

 
K3 _____MWKLLPAASA
C16 SAAPGEPCRLLAGVE
S58 NFPVTSLSQTDEIPT
T60 PVTSLSQTDEIPTLT
S195 TQPPEIESFYPPIDE
Y197 PPEIESFYPPIDEPA
K208 DEPAIGNKSVDLSGR
K233 TFVFLNAKQHKKLGS
T278 VDVGITNTQLIITDS
L292 SQRKWIHLIMDILQR
K334 GQPCTEVKTTTPGPS
T335 QPCTEVKTTTPGPSL
T337 CTEVKTTTPGPSLSQ
S341 KTTTPGPSLSQGLSA
S343 TTPGPSLSQGLSANG
S347 PSLSQGLSANGKVIP
G389 PEEVKIFGLDQNSRK
L398 DQNSRKLLQGTCNIK
T401 SRKLLQGTCNIKETS
N403 KLLQGTCNIKETSNQ
S419 SNSNNAASNTLVRGK
T421 SNNAASNTLVRGKAP
A427 NTLVRGKAPNYQLsP
N429 LVRGKAPNYQLsPMK
Y430 VRGKAPNYQLsPMKC
S433-p KAPNYQLsPMKCPAA
M435 PNYQLsPMKCPAASK
A439 LsPMKCPAASKNKDW
K442 MKCPAASKNKDWSSQ
N443 KCPAASKNKDWSSQQ
- under review  
C462 IKNYFQPCSRKRERD
S488 SRVELSCSLLEQTQP
G497 LEQTQPAGPSLWKSK
K504 GPSLWKSKDHESQSE
S508 WKSKDHESQSETLDR
R515 SQSETLDRASNASSV
S517 SETLDRASNASSVGG
S533 DIKPNGKSPDSKSFS
D543 SKSFSTEDLRARKRK
R549 EDLRARKRKEVDLST
R566 EVLEELLRSTKPELA
H600 MDAERNQHLNGGPVP
N602 AERNQHLNGGPVPES
P605 NQHLNGGPVPESNSA
S609 NGGPVPESNSALQED
N610 GGPVPESNSALQEDE
L613 VPESNSALQEDETGK
H663 EFRSLVVHNNSSRNL
H663 EFRSLVVHNNSSRNL
C671 NNSSRNLCVLNGRGE
K680 LNGRGELKNFKKFKK
K687 KNFKKFKKATCPGAG
C690 KKFKKATCPGAGKLP
K695 ATCPGAGKLPHIIGG
S703 LPHIIGGSDLIGHHA
K712 LIGHHARKNTELEEW
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