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Protein Page:
NBS1 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
gl O-GlcNAc
ga O-GalNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
NBS1 a member of the MRE11/RAD50 double-strand break repair complex. Involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. Mutation results in the Nijmegen breakage syndrome (NBS), an autosomal recessive chromosomal instability syndrome. Note: This description may include information from UniProtKB.
Protein type: DNA repair, damage; Cell cycle regulation
Cellular Component: nucleoplasm; PML body; Mre11 complex; nuclear chromosome, telomeric region; nucleolus; replication fork; nucleus; nuclear inclusion body
Molecular Function: ATP-dependent DNA helicase activity; protein binding; damaged DNA binding; protein N-terminus binding; transcription factor binding
Biological Process: meiosis; DNA damage response, signal transduction by p53 class mediator; mitotic cell cycle checkpoint; positive regulation of kinase activity; isotype switching; DNA repair; positive regulation of protein amino acid autophosphorylation; double-strand break repair via homologous recombination; DNA duplex unwinding; regulation of DNA replication initiation; cell proliferation; double-strand break repair; mitotic cell cycle G2/M transition DNA damage checkpoint; DNA damage checkpoint; blastocyst growth; cell cycle arrest; neuromuscular process controlling balance; telomere maintenance
Reference #:  O60934 (UniProtKB)
Alt. Names/Synonyms: AT-V1; AT-V2; ATV; Cell cycle regulatory protein p95; FLJ10155; MGC87362; NBN; NBS; NBS1; Nibrin; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; P95; p95 protein of the MRE11/RAD50 complex
Gene Symbols: NBN
Molecular weight: 84,959 Da
Basal Isoelectric point: 6.5  Predict pI for various phosphorylation states
CST Pathways:  G2/M DNA Damage Checkpoint  |  Protein Acetylation
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NBS1

Protein Structure Not Found.


STRING  |  Scansite  |  Phospho.ELM  |  NetworKIN  |  Pfam  |  DISEASE  |  GeneCards  |  UniProtKB  |  Entrez-Gene  |  GenPept  |  Ensembl Gene


Sites Implicated In
apoptosis, induced: S278‑p, S343‑p
cell cycle regulation: S278‑p, S343‑p
cell growth, altered: S343‑p
chromatin organization, altered: S278‑p, S343‑p
acetylation: S343‑p
activity, induced: S343‑p
phosphorylation: S278‑p, S343‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       human

 
0 1 Y16-p GPAGGEPyRLLTGVE
0 10 S58-p NFSVTNLsQtDEIPV
0 2 T60-p SVTNLsQtDEIPVLT
0 1 S195-p KQPPQIEsFyPPLDE
0 1 Y197-p PPQIEsFyPPLDEPS
1 0 K208-a DEPSIGSkNVDLSGR
1 0 K233-a TFIFLNAkQHKKLSS
9 0 S278-p VDTGITNsQTLIPDC
1 0 K334-a GHPSTGLkTTtPGPs
0 4 T337-p STGLkTTtPGPsLsQ
0 3 S341-p kTTtPGPsLsQGVsV
40 55 S343-p TtPGPsLsQGVsVDE
0 4 S347-p PsLsQGVsVDEKLMP
1 68 S397-p EQKFRMLsQDAPtVK
0 1 A400 FRMLsQDAPtVKESC
0 1 T402-p MLsQDAPtVKESCKT
0 1 Y429-p AKMRIPNyQLsPtKL
1 13 S432-p RIPNyQLsPtKLPsI
0 2 T434-p PNyQLsPtKLPsINk
0 1 S438-p LsPtKLPsINksKDR
1 0 K441-a tKLPsINksKDRASQ
0 2 S442-p KLPsINksKDRASQQ
0 1 S462-p IRNYFQPsTKKRERD
0 7 T497-p LEQTQPAtPSLWKNk
1 0 K504-a tPSLWKNkEQHLsEN
0 3 S509-p KNkEQHLsENEPVDt
0 1 T516-p sENEPVDtNsDNNLF
0 1 S518-p NEPVDtNsDNNLFTD
0 2 N534 DLKSIVKNSASKSHA
1 3 K544-a SKSHAAEkLRSNKKR
0 2 S604-p IETNDTFsDEAVPEs
0 1 A607 NDTFsDEAVPEssKI
0 1 S611-p sDEAVPEssKIsQEN
0 4 S612-p DEAVPEssKIsQENE
1 12 S615-p VPEssKIsQENEIGK
1 0 K665-a EFRSLVIkNSTSRNP
0 1 K665-u EFRSLVIkNSTSRNP
0 1 S673-p NSTSRNPsGINDDYG
0 2 K683-u NDDYGQLkNFKKFKk
1 0 K690-a kNFKKFKkVTYPGAG
1 0 K698-a VTYPGAGkLPHIIGG
1 0 K715-a LIAHHARkNTELEEW
3001 : Phospho-p95/NBS1 (Ser343) Antibody
  mouse

 
Y16 GAAPGEPYRLLAGVE
S58 NFPVTSLSQTDEIPT
T60 PVTSLSQTDEIPTLT
S195 KQPPDIESFYPPIDE
Y197 PPDIESFYPPIDEPA
K208 DEPAIGSKSVDLSGR
K233 TFVFLNAKQHKKLSS
T278-p VDVGITNtQLIISHS
K334 GQPCTELKTTTPGPS
T337 CTELKTTTPGPSLSQ
S341 KTTTPGPSLSQVLSA
S343 TTPGPSLSQVLSANG
S347 PSLSQVLSANGKIIP
S398-p EQSSRKLsQEtFNIK
T401-p SRKLsQEtFNIKEAP
N403 KLsQEtFNIKEAPKP
Y430 VRGKTPSYQLsPMKF
S433-p KTPSYQLsPMKFPVA
M435 PSYQLsPMKFPVANK
V439 LsPMKFPVANKNKDW
K442 MKFPVANKNKDWTSQ
N443 KFPVANKNKDWTSQQ
C462 IKNYFQPCTRKRERD
G497 LEQTQPAGPSLWKSK
K504 GPSLWKSKEHQSQNA
S508 WKSKEHQSQNATLDR
R515 SQNATLDREADTSSV
A517 NATLDREADTSSVGG
S533-p DIELNRKsPDRKPLP
T543 RKPLPTETLRPRKRK
N602 AERNRPLNGGsEPES
S605-p NRPLNGGsEPESNSA
S609 NGGsEPESNSALQED
N610 GGsEPESNSALQEDE
L613 EPESNSALQEDEREK
H665 RSLVVSNHNSTSRNL
H665 RSLVVSNHNSTSRNL
- gap
K681 VNECGPLKNFKKFKK
K688 KNFKKFKKATFPGAG
K696 ATFPGAGKLPHIIGG
K713 LVGHHARKNTELEEW
  rat

 
C16 SAAPGEPCRLLAGVE
S58 NFPVTSLSQTDEIPT
T60 PVTSLSQTDEIPTLT
S195 TQPPEIESFYPPIDE
Y197 PPEIESFYPPIDEPA
K208 DEPAIGNKSVDLSGR
K233 TFVFLNAKQHKKLGS
T278 VDVGITNTQLIITDS
K334 GQPCTEVKTTTPGPS
T337 CTEVKTTTPGPSLSQ
S341 KTTTPGPSLSQGLSA
S343 TTPGPSLSQGLSANG
S347 PSLSQGLSANGKVIP
L398 DQNSRKLLQGTCNIK
T401 SRKLLQGTCNIKETS
N403 KLLQGTCNIKETSNQ
Y430 VRGKAPNYQLSPMKC
S433 KAPNYQLSPMKCPAA
M435 PNYQLSPMKCPAASK
A439 LSPMKCPAASKNKDW
K442 MKCPAASKNKDWSSQ
N443 KCPAASKNKDWSSQQ
C462 IKNYFQPCSRKRERD
G497 LEQTQPAGPSLWKSK
K504 GPSLWKSKDHESQSE
S508 WKSKDHESQSETLDR
R515 SQSETLDRASNASSV
S517 SETLDRASNASSVGG
S533 DIKPNGKSPDSKSFS
D543 SKSFSTEDLRARKRK
N602 AERNQHLNGGPVPES
P605 NQHLNGGPVPESNSA
S609 NGGPVPESNSALQED
N610 GGPVPESNSALQEDE
L613 VPESNSALQEDETGK
H663 EFRSLVVHNNSSRNL
H663 EFRSLVVHNNSSRNL
- gap
K680 LNGRGELKNFKKFKK
K687 KNFKKFKKATCPGAG
K695 ATCPGAGKLPHIIGG
K712 LIGHHARKNTELEEW
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