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Protein Page:
PERK (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PERK a transmembrane protein kinase of the PEK family resident in the endoplasmic reticulum (ER) membrane and is linked to insulin processing.. Couples ER stress to translation inhibition. Stress induces autophosphorylation of its kinase domain and increases its activity. Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (eIF2alpha), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. A critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin d1. Forms dimers with BiP in resting cells. Oligomerizes in ER-stressed cells. LOF mutations cause Wolcott-Rallison syndrome (WRS), characterized by insulin-dependent diabetes in early infancy and, later, multiple system abnormalities. Neuronal death in Alzheimer?s and Parkinson?s diseases is thought to be due to ER stress and has been weakly linked to PEK. Note: This description may include information from UniProtKB.
Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Translation; Membrane protein, integral; EC 2.7.11.1; Kinase, protein; Other group; PEK family; PEK subfamily
Cellular Component: endoplasmic reticulum membrane; membrane; cell; endoplasmic reticulum; cytoplasm; integral to membrane; intracellular
Molecular Function: transferase activity; protein serine/threonine kinase activity; identical protein binding; protein binding; enzyme binding; translation initiation factor activity; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; protein phosphatase binding; ATP binding; eukaryotic translation initiation factor 2alpha kinase activity; protein kinase activity
Biological Process: fat cell differentiation; lactation; positive regulation of protein binding; translation; protein amino acid autophosphorylation; protein amino acid phosphorylation; bone mineralization; regulation of translation; negative regulation of translation in response to stress; pancreas development; regulation of fatty acid metabolic process; translational initiation; response to stress; skeletal development; ER overload response; protein homooligomerization; caspase activation; endoplasmic reticulum organization and biogenesis; ossification; positive regulation of signal transduction; negative regulation of myelination; unfolded protein response; calcium-mediated signaling; chondrocyte development; SREBP-mediated signaling pathway; endocrine pancreas development; response to unfolded protein; insulin-like growth factor receptor signaling pathway; negative regulation of translation; phosphorylation; negative regulation of apoptosis
Reference #:  Q9Z2B5 (UniProtKB)
Alt. Names/Synonyms: AI427929; E2AK3; Eif2ak3; eukaryotic translation initiation factor 2 alpha kinase 3; Eukaryotic translation initiation factor 2-alpha kinase 3; Pancreatic eIF2-alpha kinase; Pek; Perk; PRKR-like endoplasmic reticulum kinase
Gene Symbols: Eif2ak3
Molecular weight: 124,682 Da
Basal Isoelectric point: 5.13  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PERK

Protein Structure Not Found.


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Sites Implicated In
apoptosis, induced: T799‑p
translation, altered: Y615‑p
enzymatic activity, induced: Y615‑p
enzymatic activity, inhibited: T799‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
0 1 T173 SMEAVPFTVESLLES
0 2 Y264 VGHFELRYIPDMETR
0 1 T437 LIHSPSRTPVLVGSD
0 1 S443 RTPVLVGSDEFDKCL
0 1 S451 DEFDKCLSNDKYSHE
0 1 Y460 DKYSHEEYSNGALSI
0 1 Y470 GALSILQYPYDNGYY
0 1 Y476 QYPYDNGYYLPYYKR
0 2 Y477 YPYDNGYYLPYYKRE
0 1 Y480 DNGYYLPYYKRERNK
0 2 Y481 NGYYLPYYKRERNKR
0 1 S489-p KRERNKRstQItVRF
0 1 T490-p RERNKRstQItVRFL
0 1 T493-p NKRstQItVRFLDSP
0 14 S551-p PHRQRKEsETQCQTE
0 2 T553 RQRKEsETQCQTESK
0 2 T557 EsETQCQTESKyDSV
1 0 Y561-p QCQTESKyDSVSADV
0 1 S563 QTESKyDSVSADVSD
1 0 Y581 NDMKYSGYVSRYLTD
4 0 Y615-p NKVDDCNyAIKRIRL
0 1 K618 DDCNyAIKRIRLPNR
0 1 E665 PPEKWQEEMDEIWLK
0 1 S684 DWPLSSPSPMDAPSV
0 1 S690 PSPMDAPSVKIRRMD
0 3 T701 RRMDPFSTKEQIEVI
0 8 S711-p QIEVIAPsPERSRSF
0 3 S715 IAPsPERSRSFSVGI
1 1 T799-p YTRSREGtSSSIVFE
0 1 S800 TRSREGtSSSIVFED
0 2 S801 RSREGtSSSIVFEDS
0 1 S841 LTDLKCSSSRSSSEA
0 2 S842 TDLKCSSSRSSSEAT
0 1 S852 SSEATTLSTSPTRPT
0 1 S854 EATTLSTSPTRPTTL
8 0 T980-p MPAYATHtGQVGTKL
0 4 S1090-p KTVLRQRsRsMsSSG
1 12 S1092-p VLRQRsRsMsSSGTK
0 6 S1094-p RQRsRsMsSSGTKHS
0 8 S1105-p TKHSRQPsCSySPLP
0 1 S1107 HSRQPsCSySPLPGN
0 2 Y1108-p SRQPsCSySPLPGN_
0 1 S1109 RQPsCSySPLPGN__
3179 : Phospho-PERK (Thr980) (16F8) Rabbit mAb
  human

 
T177-p SMETVPFtVESLLES
Y268-p VGHFELRyIPDMETR
T441-p LIHSPSRtPVLVGsD
S447-p RtPVLVGsDEFDKCL
S455-p DEFDKCLsNDKFSHE
Y464-p DKFSHEEySNGALSI
Y474-p GALSILQyPYDNGyy
Y480-p QyPYDNGyyLPyyKR
Y481-p yPYDNGyyLPyyKRE
Y484-p DNGyyLPyyKRERNK
Y485-p NGyyLPyyKRERNKR
S493 KRERNKRSTQITVRF
T494 RERNKRSTQITVRFL
T497 NKRSTQITVRFLDNP
S555-p PHRQRKEsEtQCQtE
T557-p RQRKEsEtQCQtENK
T561-p EsEtQCQtENKYDsV
Y565 QCQtENKYDsVSGEA
S567-p QtENKYDsVSGEAND
Y585-p NDIKNSGyISRYLTD
Y619-p NKVDDCNyAIkRIRL
K622-ub DDCNyAIkRIRLPNR
K669-ub PPEKWQEkMDEIWLK
S688-p DWPLSSPsPMDAPsV
S694-p PsPMDAPsVKIRRMD
T705-p RRMDPFAtKEHIEII
S715-p HIEIIAPsPQRsRSF
S719-p IAPsPQRsRSFSVGI
T802-p YVRSRERtssSIVFE
S803-p VRSRERtssSIVFED
S804-p RSRERtssSIVFEDS
S844-p LTAFKPTssKSSSEA
S845-p TAFKPTssKSSSEAT
S854-p SSSEATLsIsPPRPT
S856-p SEATLsIsPPRPTTL
T982-p MPAYARHtGQVGTKL
S1092 KTVLRQRSRsLsSSG
S1094-p VLRQRSRsLsSSGTK
S1096-p RQRSRsLsSSGTKHS
N1107 TKHSRQSNNsHsPLP
S1109-p HSRQSNNsHsPLPSN
H1110 SRQSNNsHsPLPSN_
S1111-p RQSNNsHsPLPSN__
  rat

 
T172 SMEAVPFTVESLLES
Y263 VGHFELRYIPDMETR
T433 LIHSPSRTPVLVGSD
S439 RTPVLVGSDEFDKCL
S447 DEFDKCLSNDKYSHE
Y456 DKYSHEEYSNGALSI
Y466 GALSILQYPYDNGYY
Y472 QYPYDNGYYLPYYKR
Y473 YPYDNGYYLPYYKRE
Y476 DNGYYLPYYKRERNK
Y477 NGYYLPYYKRERNKR
S485 KRERNKRSTQITVRF
T486 RERNKRSTQITVRFL
T489 NKRSTQITVRFLDSP
S547 PHRQRKESETQCQTE
T549 RQRKESETQCQTESK
T553 ESETQCQTESKYDSV
Y557 QCQTESKYDSVSADN
S559 QTESKYDSVSADNSD
Y577 NDIKHSGYVSRYLTD
Y611 NKVDDCNYAIKRIRL
K614 DDCNYAIKRIRLPNR
E661 PPEKWQEEMDEIWLK
S680 DWPLSSPSPMDAPSV
S686 PSPMDAPSVKIRQMD
T697 RQMDPFSTKEQIEVI
S707 QIEVIAPSPERSRSF
S711 IAPSPERSRSFSVGI
T794 YTRSREGTSSSIVFE
S795 TRSREGTSSSIVFED
S796 RSREGTSSSIVFEDS
S836 LTEFKHSSSRSSSEA
S837 TEFKHSSSRSSSEAT
S846 SSSEATLSTSPTRPT
S848 SEATLSTSPTRPTTL
T974 MPAYATHTGQVGTKL
S1084 KTVLRQRSRSLSSSG
S1086 VLRQRSRSLSSSGTK
S1088 RQRSRSLSSSGTKHS
S1099 TKHSRQPSSTFSPLP
T1101 HSRQPSSTFSPLPGN
F1102 SRQPSSTFSPLPGN_
- under review  
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