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Protein Page:
MSK2 (human)
p Phosphorylation
a Acetylation
m Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
u Ubiquitination
s Sumoylation
n Neddylation
g O-GlcNAc
h Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage

Overview
MSK2 an AGC kinase of the RSK family. Has two kinase domains connected by a regulatory linker region. Phosphorylates H3 and HMG-14 in response to growth factors and cellular stress. Note: This description may include information from UniProtKB.
Protein type: EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; AGC group; RSK family; MSK subfamily
Cellular Component: cytoplasm; nucleolus; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; mitogen-activated protein kinase p38 binding; magnesium ion binding; ribosomal protein S6 kinase activity; ATP binding
Biological Process: axon guidance; positive regulation of histone phosphorylation; regulation of transcription, DNA-dependent; histone phosphorylation; negative regulation of cytokine production; activation of CREB transcription factor; protein kinase cascade; positive regulation of histone acetylation; positive regulation of transcription from RNA polymerase II promoter; inflammatory response; protein amino acid phosphorylation; activation of NF-kappaB transcription factor
Reference #:  O75676 (UniProtKB)
Alt. Names/Synonyms: 90 kDa ribosomal protein S6 kinase 4; KS6A4; mitogen- and stress-activated protein kinase 2; MSK2; Nuclear mitogen- and stress-activated protein kinase 2; Ribosomal protein kinase B; ribosomal protein S6 kinase alpha 4; Ribosomal protein S6 kinase alpha-4; ribosomal protein S6 kinase, 90kDa, polypeptide 4; RPS6KA4; RSK-B; RSKB; S6K-alpha-4
Gene Symbols: RPS6KA4
Molecular weight: 85,606 Da
Basal Isoelectric point: 8.51  Predict pI for various phosphorylation states
CST Pathways:  GPCRs Signaling to MAPK/Erk  |  MAPK/Erk in Growth and Differentiation  |  Signaling Pathways Activating p38 MAPK
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

MSK2
Protein Structure Not Found.


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Sites Implicated In
enzymatic activity, induced: S196‑p, S324‑p, S343‑p, S347‑p, S360‑p, T568‑p, T687‑p
phosphorylation: S324‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


  MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


        human

► Hide Isoforms 		 
0 1 S29-p TGHEEKVsVENFELL
0 2 K37-u VENFELLkVLGtGAy
0 1 T41-p ELLkVLGtGAyGKVF
0 2 Y44-p kVLGtGAyGKVFLVR
0 1 Y141-p KEAEVRVyGGEIVLA
0 2 R160 HKLGIIYRDLKLENV
0 1 K191-u KEFLTEEkERtFsFC
0 1 T194-p LTEEkERtFsFCGTI
4 1 S196-p EEkERtFsFCGTIEY
1 0 S324-p PFRPQIRsELDVGNF
0 1 Y342-p FTRLEPVysPPGsPP
1 16 S343-p TRLEPVysPPGsPPP
1 15 S347-p PVysPPGsPPPGDPR
0 2 Y359-p DPRIFQGysFVAPsI
1 10 S360-p PRIFQGysFVAPsIL
0 5 S365-p GysFVAPsILFDHNN
0 3 T376-p DHNNAVMtDGLEAPG
0 1 K440-u SGQEFAVkILSRRLE
0 1 S502-p IRKKRHFsESEASQI
0 1 S504 KKRHFsESEASQILR
0 1 S507 HFsESEASQILRSLV
0 1 K532-u GVVHRDLkPENILYA
0 1 T542-p NILYADDtPGAPVkI
0 1 K548-u DtPGAPVkIIDFGFA
1 0 T568-p SPGVPMQtPCFTLQY
0 1 S634-p KIREGRFsLDGEAWQ
0 4 S678-p SSWLQDGsARSsPPL
0 1 S681 LQDGsARSsPPLRtP
0 3 S682-p QDGsARSsPPLRtPD
1 14 T687-p RSsPPLRtPDVLESS
0 1 S721-p REGFFLKsVENAPLA
0 1 S737-p RRKQKLRsATASRRG
0 6 S745-p ATASRRGsPAPANPG
0 1 S758 PGRAPVASKGAPRRA
0 1 P770 RRANGPLPPS_____
  MSK2 iso2  
S29 TGHEEKVSVENFELL
K37 VENFELLKVLGTGAY
T41 ELLKVLGTGAYGKVF
Y44 KVLGTGAYGKVFLVR
Y141 KEAEVRVYGGEIVLA
R160 HKLGIIYRDLKLENV
K191 KEFLTEEKERTFSFC
T194 LTEEKERTFSFCGTI
S196 EEKERTFSFCGTIEY
S324 PFRPQIRSELDVGNF
Y342 FTRLEPVYSPPGSPP
S343 TRLEPVYSPPGSPPP
S347 PVYSPPGSPPPGDPR
Y359 DPRIFQGYSFVAPSI
S360 PRIFQGYSFVAPSIL
S365 GYSFVAPSILFDHNN
T376 DHNNAVMTDGLEAPG
K434 SGQEFAVKILSRRLE
S496 IRKKRHFSESEASQI
S498 KKRHFSESEASQILR
S501 HFSESEASQILRSLV
K526 GVVHRDLKPENILYA
T536 NILYADDTPGAPVKI
K542 DTPGAPVKIIDFGFA
T562 SPGVPMQTPCFTLQY
S628 KIREGRFSLDGEAWQ
S672 SSWLQDGSARSSPPL
S675 LQDGSARSSPPLRTP
S676 QDGSARSSPPLRTPD
T681 RSSPPLRTPDVLESS
S715 REGFFLKSVENAPLA
S731 RRKQKLRSATASRRG
S739 ATASRRGSPAPANPG
S752 PGRAPVASKGAPRRA
P764 RRANGPLPPS_____
  mouse

 
S29 TGHEEKVSVENFALL
K37 VENFALLKVLGTGAY
T41 ALLKVLGTGAYGKVF
Y44 KVLGTGAYGKVFLVR
Y141 KEAEVRVYGGEIVLA
R160-m1 HKLGIIYrDLKLENV
K191 KEFLTEEKERTFSFC
T194 LTEEKERTFSFCGTI
S196 EEKERTFSFCGTIEY
S324 PFRPQIRSELDVGNF
Y342 FTRLEPVYsPAGsPP
S343-p TRLEPVYsPAGsPPP
S347-p PVYsPAGsPPPGDPR
Y359 DPRIFQGYSFVAPSI
S360 PRIFQGYSFVAPSIL
S365 GYSFVAPSILFDHNN
A376 DHNNAVMADVLQAPG
K440 SGQEFAVKILSRRLE
S502-p IRKKRLFsEsEAsQI
S504-p KKRLFsEsEAsQILR
S507-p LFsEsEAsQILRSLV
K532 GVVHRDLKPENILYA
T542 NILYADDTPGAPVKI
K548 DTPGAPVKIIDFGFA
T568 SPAEPMQTPCFTLQY
S634 KIREGRFSLDGEAWQ
S678 SSWLQDGSARssPPL
S681-p LQDGSARssPPLRtP
S682-p QDGSARssPPLRtPD
T687-p RssPPLRtPDVLESS
S721 REGFFLKSVENAPLA
S737 RRKQKLRSAAASRRG
S745-p AAASRRGsPVPASSG
S757-p SSGRLPAsAAKGTTR
S771-p RRANGPLsPS_____
  rat

 
S29 TGHEEKVSVENFALL
K37 VENFALLKVLGTGAY
T41 ALLKVLGTGAYGKVF
Y44 KVLGTGAYGKVFLVR
Y141 KEAEVRVYGGEIVLA
R160 HKLGIIYRDLKLENV
K191 KEFLTEEKERTFSFC
T194 LTEEKERTFSFCGTI
S196 EEKERTFSFCGTIEY
S324 PFRPQIRSELDVGNF
Y342 FTRLEPVYSPAGSPP
S343 TRLEPVYSPAGSPPP
S347 PVYSPAGSPPPGDPR
Y359 DPRIFQGYSFVAPSI
S360 PRIFQGYSFVAPSIL
S365 GYSFVAPSILFDHNN
A376 DHNNAVMADVLAAPG
K440 SGQEFAVKILSRRLE
S502 IRKKRLFSESEASQI
S504 KKRLFSESEASQILR
S507 LFSESEASQILRSLV
K532 GVVHRDLKPENILYA
T542 NILYADDTPGAPVKI
K548 DTPGAPVKIIDFGFA
T568 SPAGPMQTPCFTLQY
S634 KIREGRFSLDGEAWQ
S678 SSWLQDGSARSSPPL
S681 LQDGSARSSPPLRTP
S682 QDGSARSSPPLRTPD
T687 RSSPPLRTPDVLESS
S721 REGFFLKSVENAPLA
S737 RRKQKLRSAAASRRG
S745 AAASRRGSPVPASSG
S757 SSGRLPASASKGTTR
S771 RRANGPLSPS_____
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