Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
PIK3R1 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PIK3R1 regulatory subunit of phosphoinositide-3-kinase. Mediates binding to a subset of tyrosine-phosphorylated proteins through its SH2 domain. Acts as an adapter, mediating the association of the p110 catalytic unit of the alpha, beta and delta enzymes to the plasma membrane, where p110 phosphorylates inositol lipids. May play an additional role in the regulation of the actin cytoskeleton. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Its SH2 domains interacts with the YTHM motif of phosphorylated INSR in vitro. Defects in PIK3R1 are a cause of severe insulin resistance. Four alternatively spliced isoforms have been described. Note: This description may include information from UniProtKB.
Protein type: Enzyme, regulatory subunit; Kinase, lipid; Motility/polarity/chemotaxis
Cellular Component: neuron projection; protein complex; membrane; cell; cytoplasm; plasma membrane; intercellular junction; intracellular; nucleus; cytosol; phosphoinositide 3-kinase complex
Molecular Function: protein C-terminus binding; protein domain specific binding; neurotrophin TRKA receptor binding; 1-phosphatidylinositol-3-kinase activity; platelet-derived growth factor receptor binding; phosphoprotein binding; phosphoinositide 3-kinase regulator activity; 1-phosphatidylinositol-3-kinase regulator activity; receptor tyrosine kinase binding; protein phosphatase binding; protein kinase binding; ATPase binding; insulin receptor binding; insulin binding; calmodulin binding; transferase activity; insulin-like growth factor receptor binding; protein binding; ErbB-3 class receptor binding; ubiquitin protein ligase binding; insulin receptor substrate binding; estrogen receptor binding; phosphoinositide 3-kinase binding; kinase activity; receptor binding
Biological Process: negative regulation of osteoclast differentiation; phosphoinositide 3-kinase cascade; response to cAMP; negative regulation of heart rate; negative regulation of smooth muscle cell proliferation; response to glucocorticoid stimulus; signal transduction; protein amino acid phosphorylation; regulation of phosphoinositide 3-kinase activity; NFAT protein import into nucleus; response to insulin stimulus; phosphoinositide phosphorylation; negative regulation of blood pressure; negative regulation of proteolysis; glucose metabolic process; positive regulation of tumor necrosis factor production; negative regulation of cell-matrix adhesion; positive regulation of myoblast differentiation; DNA damage response, signal transduction resulting in induction of apoptosis; insulin-like growth factor receptor signaling pathway; induction of apoptosis via death domain receptors; B cell differentiation; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; phosphorylation; negative regulation of apoptosis; positive regulation of cell migration
Reference #:  P26450 (UniProtKB)
Alt. Names/Synonyms: AA414921; C530050K14; p50alpha; p55alpha; P85A; p85alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K; PI3K regulatory subunit alpha; Pik3r1; PtdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-alpha
Gene Symbols: Pik3r1
Molecular weight: 83,517 Da
Basal Isoelectric point: 5.9  Predict pI for various phosphorylation states
CST Pathways:  Actin Dynamics  |  Adherens Junction Dynamics  |  AMPK Signaling  |  Angiogenesis  |  Apoptosis Regulation  |  B Cell Receptor Signaling  |  ErbB/HER Signaling  |  ESC Pluripotency and Differentiation  |  Growth And Differentiation Control by MAPKs  |  IL6 Signaling  |  Inhibition of Apoptosis  |  Insulin Receptor Signaling  |  Microtubule Dynamics  |  Mitochondrial Control of Apoptosis  |  mTOR Signaling  |  NF-kB Signaling  |  PI3K/Akt Signaling  |  Protein Kinase C Signaling  |  SAPK/JNK Signaling Cascades  |  T Cell Receptor Signaling  |  TGF-├č Signaling  |  Translation: eIF4E and p70S6K  |  Warburg Effect
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PIK3R1

Protein Structure Not Found.


STRING  |  Reactome  |  BioGPS  |  Scansite  |  Pfam  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Sites Implicated In
signaling pathway regulation: S361‑p, S652‑p
molecular association, regulation: S361‑p, S652‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
0 1 T31 LHLGDILTVNKGSLV
0 1 Y59 DIGWLNGYNETTGER
0 1 Y73 RGDFPGTYVEYIGRK
0 3 Y76 FPGTYVEYIGRKRIS
3 1 S83 YIGRKRISPPTPKPR
1 0 T86 RKRISPPTPKPRPPR
0 1 S147 EKKGLECSTLYRTQs
0 1 T148 KKGLECSTLYRTQsS
0 1 T152 ECSTLYRTQsSSNPA
1 2 S154-p STLYRTQsSSNPAEL
1 0 Y203 PVIPVAVYNEMMSLA
0 1 S265 LLNARVLSEIFSPVL
0 1 S279 LFRFPAASSDNTEHL
1 1 S361-p TFLVRDAsTKMHGDY
2 2 Y368 sTKMHGDYTLTLRKG
0 1 T369 TKMHGDYTLTLRKGG
0 1 K382-ub GGNNKLIkIFHRDGK
0 1 Y416 RNESLAQYNPKLDVK
0 8 Y426-p KLDVKLLyPVSKYQQ
0 443 Y452-p VGKKLHEyNTQFQEK
0 2 T454 KKLHEyNTQFQEKSR
0 16 Y463 FQEKSREYDRLyEEy
8 2178 Y467-p SREYDRLyEEyTRTS
0 39 Y470-p YDRLyEEyTRTSQEI
0 5 Y504 QCQTQERYSKEyIEK
2 32 Y508-p QERYSKEyIEKFKRE
1 8 H528 IQRIMHNHDKLKSRI
0 1 K530 RIMHNHDKLKSRISE
1 29 Y556 LKKQAAEYREIDKRM
0 15 T576-p DLIQLRKtRDQyLMW
1 896 Y580-p LRKtRDQyLMWLTQK
0 2 T603 EWLGNENTEDQysLV
1 240 Y607-p NENTEDQysLVEDDE
3 24 S608-p ENTEDQysLVEDDED
0 1 S628 EKTWNVGSSNRNKAE
0 1 S629 KTWNVGSSNRNKAEN
1 1 S652-p TFLVRESsKQGCyAC
0 1 Y657-p ESsKQGCyACSVVVD
0 14 Y679-p INKTATGyGFAEPYN
3 0 Y688 FAEPYNLYSsLKELV
1 0 S690-p EPYNLYSsLKELVLH
4228 : Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody
  human

 
T31 LHLGDILTVNKGSLV
Y59-p EIGWLNGyNETTGER
Y73-p RGDFPGTyVEyIGRK
Y76-p FPGTyVEyIGRKKIs
S83-p yIGRKKIsPPTPKPR
T86 RKKIsPPTPKPRPPR
S147-p EKKGLECstLYRtQs
T148-p KKGLECstLYRtQsS
T152-p ECstLYRtQsSSNLA
S154-p stLYRtQsSSNLAEL
Y203-p PVIPAAVySEMISLA
S265-p LLNARVLsEIFSPML
S279-p LFRFSAAsSDNTENL
S361 TFLVRDASTKMHGDy
Y368-p STKMHGDytLTLRKG
T369-p TKMHGDytLTLRKGG
K382 GGNNKLIKIFHRDGK
Y416 RNESLAQYNPKLDVK
Y426-p KLDVKLLyPVSKYQQ
Y452-p VGKKLHEyNtQFQEK
T454-p KKLHEyNtQFQEKSR
Y463-p FQEKSREyDRLyEEy
Y467-p SREyDRLyEEyTRTS
Y470-p yDRLyEEyTRTSQEI
Y504-p QCQTQERySKEyIEK
Y508-p QERySKEyIEKFKRE
Y528-p IQRIMHNyDkLKSRI
K530-ac RIMHNyDkLKSRISE
Y556-p LKKQAAEyREIDKRM
T576-p DLIQLRKtRDQyLMW
Y580-p LRKtRDQyLMWLTQK
T603-p EWLGNENtEDQysLV
Y607-p NENtEDQysLVEDDE
S608-p ENtEDQysLVEDDED
S628-p EKTWNVGssNRNKAE
S629-p KTWNVGssNRNKAEN
S652 TFLVRESSKQGCyAC
Y657-p ESSKQGCyACSVVVD
Y679-p INKTATGyGFAEPYN
Y688-p FAEPYNLySSLKELV
S690 EPYNLySSLKELVLH
4228 : Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody
  rat

 
T31-p LHLGDILtVNKGSLV
Y59 DIGWLNGYNETTGER
Y73 RGDFPGTYVEYIGRK
Y76 FPGTYVEYIGRKRIS
S83 YIGRKRISPPTPKPR
T86 RKRISPPTPKPRPPR
S147 EKKGLECSTLYRTQS
T148 KKGLECSTLYRTQSS
T152 ECSTLYRTQSSSNPA
S154 STLYRTQSSSNPAEL
Y203 PVIPVAVYNEMMSLA
S265 LLNARALSEIFSHVL
S279 LFRFPAASSDNTEHL
S361 TFLVRDASTKMHGDY
Y368 STKMHGDYTLTLRKG
T369 TKMHGDYTLTLRKGG
K382 GGNNKLIKIFHRDGK
Y416-p RNESLAQyNPKLDVK
Y426-p KLDVKLLyPVSKYQQ
Y452-p VGKKLHEyNTQFQEK
T454 KKLHEyNTQFQEKSR
Y463 FQEKSREYDRLyEEY
Y467-p SREYDRLyEEYTRTS
Y470 YDRLyEEYTRTSQEI
Y504 QCHPQERYSKDyIEK
Y508-p QERYSKDyIEKFKRE
H528 IQRIMHNHDKLKSRI
K530 RIMHNHDKLKSRISE
Y556 LKKQAAEYREIDKRM
T576 DLIQLRKTRDQyLMW
Y580-p LRKTRDQyLMWLTQK
T603 EWLGNENTEDQYsLV
Y607 NENTEDQYsLVDDDE
S608-p ENTEDQYsLVDDDED
S628 EKTWNVGSSNRNKAE
S629 KTWNVGSSNRNKAEN
S652 TFLVRESSKQGCYAC
Y657 ESSKQGCYACSVVVD
Y679 INKTATGYGFAEPYN
Y688 FAEPYNLYSSLKELV
S690 EPYNLYSSLKELVLH
4228 : Phospho-PI3K p85 (Tyr458)/p55 (Tyr199) Antibody
  cow

 
T31 LHLGDILTVNKGSLV
Y59 EIGWLNGYNETTGER
Y73 RGDFPGTYVEYIGRK
Y76 FPGTYVEYIGRKKIs
S83-p YIGRKKIsPPtPKPR
T86-p RKKIsPPtPKPRPPR
S147 EKKGLECSTLYRTQS
T148 KKGLECSTLYRTQSS
T152 ECSTLYRTQSSSNPA
S154 STLYRTQSSSNPAEL
S203 PVIPVAVSSELISLA
S265 LLNARVLSELFSPLL
S279 LFRFPAASSENTEHL
S361 TFLVRDASTKMHGDy
Y368-p STKMHGDyTLTLRKG
T369 TKMHGDyTLTLRKGG
K382 GGNNKLIKIFHRDGK
Y416 RNESLAQYNPKLDVK
Y426 KLDVKLLYPVSKYQQ
Y452 VGKKLHEYNTQFQEK
T454 KKLHEYNTQFQEKSR
Y463 FQEKSREYDRLYEDY
Y467 SREYDRLYEDYTRTS
Y470 YDRLYEDYTRTSQEI
Y504 QCQTQERYSKEYIEK
Y508 QERYSKEYIEKFKRE
Y528 IQRIMHNYEKLKSRI
K530 RIMHNYEKLKSRISE
Y556 LKKQAAEYREIDKRM
T576 DLIQLRKTRDQYLMW
Y580 LRKTRDQYLMWLTQK
T603 EWLGNENTEDQYsLV
Y607 NENTEDQYsLVEDDE
S608-p ENTEDQYsLVEDDED
S628 EKTWNVGSSNRNKAE
S629 KTWNVGSSNRNKAEN
S652 TFLVRESSKQGCYAC
Y657 ESSKQGCYACSVVVD
Y679 INKTATGYGFAEPYN
Y688-p FAEPYNLySSLKELV
S690 EPYNLySSLKELVLH
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.