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Protein Page:
NFkB-p65 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
NFkB-p65 a subunit of NF-kappa-B transcription complex, which plays a crucial role in inflammatory and immune responses. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. P65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Three splice-variant isoforms have been identified. Note: This description may include information from UniProtKB.
Protein type: DNA binding protein; Transcription factor; Nuclear receptor co-regulator
Cellular Component: transcription factor complex; protein complex; cytoplasm; cytosol; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; NF-kappaB binding; identical protein binding; protein binding; enzyme binding; DNA binding; transcription activator binding; sequence-specific DNA binding; ubiquitin protein ligase binding; protein complex binding; protein N-terminus binding; chromatin binding; phosphate binding; transcription factor binding; protein kinase binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; negative regulation of insulin receptor signaling pathway; defense response; negative regulation of transcription from RNA polymerase II promoter; activation of NF-kappaB transcription factor; response to organic substance; hair follicle development; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; inflammatory response; positive regulation of Schwann cell differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; liver development; response to UV-B; regulation of transcription from RNA polymerase II promoter; organ morphogenesis; positive regulation of chondrocyte differentiation; positive regulation of interleukin-12 biosynthetic process; response to bacterium; response to muramyl dipeptide; regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of protein catabolic process; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis
Reference #:  Q04207 (UniProtKB)
Alt. Names/Synonyms: avian reticuloendotheliosis viral (v-rel) oncogene homolog A; Nfkb3; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; OTTMUSP00000017977; p65; p65 NF kappaB; p65 NF-kappa B; p65 NFkB; Rela; TF65; Transcription factor p65; v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Gene Symbols: Rela
Molecular weight: 60,212 Da
Basal Isoelectric point: 5.5  Predict pI for various phosphorylation states
CST Pathways:  Apoptosis Regulation  |  B Cell Receptor Signaling  |  Death Receptor Signaling  |  Inhibition of Apoptosis  |  NF-kB Signaling  |  Regulation of P38 MAPKs  |  T Cell Receptor Signaling  |  Toll-Like Receptor Signaling
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

NFkB-p65

Protein Structure Not Found.


STRING  |  Reactome  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho3D  |  Phospho.ELM  |  NetworKIN  |  Source  |  UCSD-Nature  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene  |  NURSA


Sites Implicated In
apoptosis, altered: S276‑p
apoptosis, inhibited: S311‑p
carcinogenesis, induced: S534‑p
cell differentiation, altered: S276‑p
transcription, altered: S276‑p, S534‑p
transcription, induced: S276‑p, S311‑p, S534‑p
transcription, inhibited: S467‑p
acetylation: S276‑p
activity, induced: S276‑p, S534‑p
molecular association, regulation: S276‑p, S311‑p, S534‑p
protein degradation: S276‑p, S467‑p, S534‑p
protein processing: S276‑p, S534‑p
ubiquitination: S467‑p

Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
1 0 K37 RGMRFRYKCEGRSAG
1 0 K37 RGMRFRYKCEGRSAG
0 1 S42 RYKCEGRSAGSIPGE
0 2 S45 CEGRSAGSIPGERST
1 0 K56 ERSTDTTKTHPTIKI
1 0 K62 TKTHPTIKINGYTGP
0 1 T71 NGYTGPGTVRISLVT
1 0 K79 VRISLVTKDPPHRPH
0 2 Y99-p GKDCRDGyYEADLCP
2 25 K122-ac NLGIQCVkkRDLEQA
1 0 K122 NLGIQCVKkRDLEQA
2 0 K123 LGIQCVkKRDLEQAI
1 1 K123-ub LGIQCVkkRDLEQAI
0 1 S131 RDLEQAISQRIQTNN
0 2 T176-p AGRPLLLtPVLSHPI
2 0 K195 APNTAELKICRVNRN
4 0 S205 RVNRNSGSCLGGDEI
3 0 K218 EIFLLCDKVQkEDIE
1 0 K218 EIFLLCDKVQkEDIE
6 0 K221-ac LLCDKVQkEDIEVYF
1 0 K221 LLCDKVQKEDIEVYF
2 1 T254 QVAIVFRTPPYADPS
0 1 Y257 IVFRTPPYADPSLQA
64 0 S276-p SMQLRRPsDRELSEP
5 0 S281 RPsDRELSEPMEFQY
0 1 T305 IEEKRKRTYETFksI
1 0 T305 IEEKRKRTYETFksI
0 1 Y306 EEKRKRTYETFksIM
0 1 T308 KRKRTYETFksIMKK
36 1 K310-ac KRTYETFksIMKKSP
1 0 K310 KRTYETFKsIMKKSP
1 0 K310 KRTYETFKsIMKKSP
9 1 S311-p RTYETFksIMKKSPF
3 0 K314 ETFksIMKKSPFNGP
2 0 K314 ETFksIMKKSPFNGP
1 0 K314 ETFksIMKKSPFNGP
3 0 K315 TFksIMKKSPFNGPT
2 0 K315 TFksIMKKSPFNGPT
1 0 K315 TFksIMKKSPFNGPT
0 1 S316 FksIMKKSPFNGPTE
1 0 T322 KSPFNGPTEPRPPTR
1 0 P352 APQPYTFPASLSTIN
2 0 T433 STQAGEGTLSEALLH
13 0 S467-p GVFTDLAsVDNSEFQ
3 0 T504-p EAITRLVtGSQRPPD
12 2 S527 SGLPNGLSGDEDFSs
106 0 S534-p SGDEDFSsIADMDFS
0 1 S545 MDFSALLSQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
12629 : Acetyl-NF-kappa-B p65 (Lys310) (D2S3J) Rabbit mAb
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
  human

 
K37-me RGMRFRYkCEGRsAG
K37-m1 RGMRFRYkCEGRsAG
S42-p RYkCEGRsAGsIPGE
S45-p CEGRsAGsIPGERST
K56-ub ERSTDTTkTHPTIkI
K62-ub TkTHPTIkINGYTGP
T71-p NGYTGPGtVRISLVT
K79-ub VRISLVTkDPPHRPH
F99 GKDCRDGFYEAELCP
K122-ac NLGIQCVkkRDLEQA
K122-ub NLGIQCVkkRDLEQA
K123-ac LGIQCVkkRDLEQAI
K123-ub LGIQCVkkRDLEQAI
S131-p RDLEQAIsQRIQTNN
P176 SGRPLRLPPVLSHPI
K195-ub APNTAELkICRVNRN
S205-p RVNRNSGsCLGGDEI
K218-ac EIFLLCDkVQkEDIE
K218-me EIFLLCDkVQkEDIE
K221-ac LLCDkVQkEDIEVYF
K221-me LLCDkVQkEDIEVYF
T254-p QVAIVFRtPPYADPS
Y257 IVFRtPPYADPSLQA
S276-p SMQLRRPsDRELsEP
S281-p RPsDRELsEPMEFQY
T305-p IEEKRKRtyEtFksI
T305-gl IEEKRKRtyEtFksI
Y306-p EEKRKRtyEtFksIM
T308-p KRKRtyEtFksIMkk
K310-ac KRtyEtFksIMkksP
K310-m1 KRtyEtFksIMkksP
K310-ub KRtyEtFksIMkksP
S311-p RtyEtFksIMkksPF
K314-ac EtFksIMkksPFSGP
K314-m1 EtFksIMkksPFSGP
K314-ub EtFksIMkksPFSGP
K315-ac tFksIMkksPFSGPt
K315-m1 tFksIMkksPFSGPt
K315-ub tFksIMkksPFSGPt
S316-p FksIMkksPFSGPtD
T322-gl ksPFSGPtDPRPPPR
T352-gl APQPYPFtSSLSTIN
T435-p PTQAGEGtLSEALLQ
S468-p AVFTDLAsVDNSEFQ
T505-p EAITRLVtGAQRPPD
S529-p GLPNGLLsGDEDFSs
S536-p sGDEDFSsIADMDFS
S547-p MDFSALLsQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
12629 : Acetyl-NF-kappa-B p65 (Lys310) (D2S3J) Rabbit mAb
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
  rat

 
K37 RGMRFRYKCEGRSAG
K37 RGMRFRYKCEGRSAG
S42 RYKCEGRSAGSIPGE
S45 CEGRSAGSIPGERST
K56 ERSTDTTKTHPTIKI
K62 TKTHPTIKINGYTGP
T71 NGYTGPGTVRISLVT
K79 VRISLVTKDPPHRPH
F99 GKDCRDGFYEAELCP
K122 NLGIQCVKKRDLEQA
K122 NLGIQCVKKRDLEQA
K123 LGIQCVKKRDLEQAI
K123 LGIQCVKKRDLEQAI
S131 RDLEQAISQRIQTNN
T176 SGRPLRLTPVLSHPI
K195 APNTAELKICRVNRN
S205 RVNRNSGSCLGGDEI
K218 EIFLLCDKVQKEDIE
K218 EIFLLCDKVQKEDIE
K221 LLCDKVQKEDIEVYF
K221 LLCDKVQKEDIEVYF
T254-p QVAIVFRtPPyADPS
Y257-p IVFRtPPyADPSLQA
S276-p SMQLRRPsDRELSEP
S281 RPsDRELSEPMEFQY
T305 IEEKRKRTYETFksI
T305 IEEKRKRTYETFksI
Y306 EEKRKRTYETFksIM
T308 KRKRTYETFksIMKK
K310-ac KRTYETFksIMKKSP
K310 KRTYETFKsIMKKSP
K310 KRTYETFKsIMKKSP
S311-p RTYETFksIMKKSPF
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K314 ETFksIMKKSPFNGP
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
K315 TFksIMKKSPFNGPT
S316 FksIMKKSPFNGPTE
T322 KSPFNGPTEPRPPPR
S352 APQPYAFSTSLSTIN
T434 STQAGEGTLSEALLH
S468-p GVFTDLAsVDNSEFQ
T505 EAITRLVTGSQRPPD
S528-p SGLPNGLsGDEDFSs
S535-p sGDEDFSsIADMDFS
S546 MDFSALLSQISS___
3045 : Acetyl-NF-kappaB p65 (Lys310) Antibody
3039 : Phospho-NF-KappaB p65 (Ser468) Antibody
3031 : Phospho-NF-KappaB p65 (Ser536) Antibody
3033 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb
3036 : Phospho-NF-KappaB p65 (Ser536) (7F1) Mouse mAb
4025 : Phospho-NF-kB p65 (Ser536) (93H1) Rabbit mAb (Biotinylated)
4886 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 488 Conjugate)
4887 : Phospho-NF-KappaB p65 (Ser536) (93H1) Rabbit mAb (Alexa Fluor(R) 647 Conjugate)
5733 : Phospho-NF-¿¿B p65 (Ser536) (93H1) Rabbit mAb (PE Conjugate)
13346 : Phospho-NF-¿B p65 (Ser536) (E1Z1T) Mouse mAb
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