Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage Cell Signaling Technology
PhosphoSitePlus
HomeAbout PhosphoSiteUsing PhosphoSiteCuration ProcessContact
NIH-logos NIGMS Logo NIAAA Logo NCI Logo NIH Logo
Protein Page:
PIWIL2 (mouse)
p Phosphorylation
ac Acetylation
me Methylation
m1 Mono-methylation
m2 Di-methylation
m3 Tri-methylation
ub Ubiquitination
sm Sumoylation
ne Neddylation
gl O-GlcNAc
ga O-GalNAc
pa Palmitoylation
ad Adenylylation
sn S-Nitrosylation
ca Caspase cleavage
sc Succinylation

Overview
PIWIL2 Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Its presence in oocytes suggests that it may participate in similar functions during oogenesis in females. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with primary piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. The piRNA process acts upstream of known mediators of DNA methylation. Participates in a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulate expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. Inhibits tumor cell growth when repressed. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors. Interacts with DDX4, MAEL, EIF3A, EIF4E, EIF4G, PRMT5 and WDR77. Associates with EIF4E- and EIF4G-containing m7G cap-binding complexes. Interacts (when methylated on arginine residues) with TDRD1. Expressed in adult testis and in most tumors. Belongs to the argonaute family. Piwi subfamily. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Protein type: Cancer Testis Antigen (CTA); Apoptosis
Cellular Component: polysome; cytoplasm
Molecular Function: mRNA binding; protein binding; nucleic acid binding; RNA binding
Biological Process: regulation of translation; oogenesis; meiosis; positive regulation of translation; multicellular organismal development; DNA methylation during gametogenesis; spermatogenesis; germ-line stem cell maintenance; RNA-mediated gene silencing; cell differentiation
Reference #:  Q8CDG1 (UniProtKB)
Alt. Names/Synonyms: CT80; Mili; Miwi like; Miwi likw; piwi like homolog 2; piwi-like homolog 2 (Drosophila); Piwi-like protein 2; Piwil1l; Piwil2; PIWL2
Gene Symbols: Piwil2
Molecular weight: 109,488 Da
Basal Isoelectric point: 9.15  Predict pI for various phosphorylation states
Protein-Specific Antibodies or siRNAs from Cell Signaling Technology® Total Proteins
Select Structure to View Below

PIWIL2

Protein Structure Not Found.


STRING  |  BioGPS  |  Scansite  |  Pfam  |  RCSB PDB  |  Phospho.ELM  |  NetworKIN  |  UniProtKB  |  Entrez-Gene  |  Ensembl Gene


Modification Sites and Domains Show Modification Legend
Click here to view phosphorylation modifications only

Modification Sites in Parent Protein, Orthologs, and Isoforms Show Modification Legend
 

Show Multiple Sequence Alignment


 SS 

SS: The number of records in which this modification site was determined using site-specific methods. SS methods include amino acid sequencing, site-directed mutagenesis, modification site-specific antibodies, specific MS strategies, etc.


 MS 

MS: The number of records in which this modification site was assigned using ONLY proteomic discovery-mode mass spectrometry.


       mouse

 
1 1 R74-m1 VGLVSMFrGMGLDTA
1 0 R74-m2 VGLVSMFrGMGLDTA
1 0 R95-m1 REVPPLGrGVLGrGL
1 0 R100-m2 LGrGVLGrGLSANMV
0 1 R144 GWSRMLGRGSSEVSL
0 1 R156 VSLLPLGRAASSIGr
1 0 R163-m2 RAASSIGrGMDKPPs
0 1 S170-p rGMDKPPsAFGLTAR
0 1 D297 KSQRKTDDAEISIKI
0 1 S301 KTDDAEISIKIQLTK
0 1 T307 ISIKIQLTKILEPCS
0 1 S314 TKILEPCSDLCIPFY
1 0 R549-m2 TASNELTrWGLSLHK
0 1 T649 LKDDRIETYIRTIQS
0 1 Y650 KDDRIETYIRTIQSL
0 1 T706-p VRTIGQPtRLRsVAQ
0 1 S710-p GQPtRLRsVAQKILL
0 1 Y770 NLTLTKWYSRVVFQM
  human

 
R76-m1 VGLVSMFrGLGIETV
R76 VGLVSMFRGLGIETV
R97 REMLPSGRGILGRGL
R102 SGRGILGRGLSANLV
R146-m1 GWSRTLGrGSSDASL
R158-m1 ASLLPLGrAAGGISR
R165 rAAGGISREVDKPPC
C172 REVDKPPCTFSTPSR
S299-p KSQRKTDsAEIsIKI
S303-p KTDsAEIsIKIQMtK
T309-p IsIKIQMtKILEPCs
S316-p tKILEPCsDLCIPFY
R551 AATNELMRWGLRLQK
T651-p LKDDRIEtyVRTIQS
Y652-p KDDRIEtyVRTIQST
T708 VRTIGQPTRLRSVAQ
S712 GQPTRLRSVAQKILL
Y772-p NLTLTKWySRVVFQM
Home  |  Curator Login With enhanced literature mining using Linguamatics I2E I2E Logo Produced by 3rd Millennium  |  Design by Digizyme
©2003-2013 Cell Signaling Technology, Inc.